Transcriptome Analysis Revealing the Interaction of Abscisic Acid and Cell Wall Modifications during the Flower Opening and Closing Process of Nymphaea lotus
Abstract
:1. Introduction
2. Results
2.1. Changes in Endogenous Hormone Content in N. lotus during Opening and Closing of the Flower
2.2. Transcriptome Profiling of the Waterlily Flower at Open and Closed Periods
2.2.1. Gene Regulation Involved in Cell Signal Transduction of Waterlily Petals
- Ca2+ Signaling
- ROS Signaling
- Light Signaling
- Hormone Coordination
2.2.2. Gene Regulation Involved in the Cell Metabolism of Waterlily Petals
- Water Transportation
- Cell Wall Modification
2.3. qRT-PCR Analysis
3. Discussion
3.1. Similarities and Differences in Flower Opening and Closing Mechanism between N. ‘Blue Bird’ and N. lotus
3.2. The Interaction between ABA Hormone and Cell Wall Modification Affected the Opening of N. lotus
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Determination of Endogenous Hormone Content during the Flower Opening and Closing Process of N. lotus
4.3. RNA-Seq, Expression Annotation, GO and KEGG Pathway Enrichment and Gene Expression Patterns
4.4. qRT-PCR Assay
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Heatmap Number | Gene ID | Best Homolog | FPKM (Close) | FPKM (Open) |
---|---|---|---|---|
1 | TRINITY_DN33954_c0_g1_i1 | CPK20 | 24.67 | 222.67 |
2 | TRINITY_DN43164_c0_g1_i7 | CBL21 | 103.08 | 5.62 |
cluster_contig14104 | CBL3 | 76.85 | 5.33 | |
TRINITY_DN43164_c0_g1_i6 | CBL23 | 176.16 | 16.00 | |
cluster_contig11726 | CBL23 | 193.56 | 21.18 | |
TRINITY_DN42937_c3_g3_i8 | CBL12 | 1537.17 | 235.54 | |
TRINITY_DN34147_c0_g1_i1 | CBL5 | 30.33 | 191.67 | |
cluster_contig33272 | CBL12 | 76.41 | 510.81 | |
cluster_contig33312 | CBL12 | 28.88 | 238.84 | |
3 | TRINITY_DN37281_c0_g1_i1 | CML49 | 25.82 | 96.47 |
TRINITY_DN43916_c0_g1_i2 | CML38 | 104.05 | 405.90 | |
cluster_contig32852 | CML50 | 29.45 | 127.22 | |
cluster_contig17156 | CML11 | 13.06 | 80.58 | |
TRINITY_DN43916_c0_g1_i1 | CML38 | 84.95 | 503.44 | |
TRINITY_DN30645_c0_g1_i1 | CML3 | 9.33 | 70.00 | |
4 | TRINITY_DN33584_c4_g1_i6 | CAM | 6.04 | 59.63 |
5 | TRINITY_DN35826_c0_g2_i1 | GPX | 246.73 | 31.54 |
6 | TRINITY_DN38584_c2_g1_i3 | GrxC | 179.65 | 31.69 |
Heatmap Number | Gene ID | Best Homolog | FPKM (Close) | FPKM (Open) |
---|---|---|---|---|
7 | cluster_contig33844 | PHOT1 | 136.62 | 576.57 |
TRINITY_DN46984_c2_g2_i1 | Phot1 | 10.07 | 60.37 | |
TRINITY_DN46984_c2_g2_i3 | PHOT1 | 98.18 | 421.90 | |
cluster_contig4683 | Phot1 | 0.00 | 35.67 | |
TRINITY_DN39051_c0_g1_i1 | PHOT1 | 35.71 | 146.36 | |
8 | cluster_contig24741 | BLUS1 | 130.88 | 566.19 |
9 | TRINITY_DN39859_c1_g2_i5 | PHYB | 2037.26 | 369.42 |
10 | cluster_contig16390 | LHY | 9279.65 | 1215.09 |
TRINITY_DN43711_c0_g2_i1 | LHY | 2875.53 | 464.13 | |
TRINITY_DN43711_c0_g2_i5 | LHY | 15,407.33 | 2664.26 | |
11 | TRINITY_DN45082_c1_g1_i9 | PIF3 | 97.68 | 485.99 |
12 | TRINITY_DN40718_c0_g1_i2 | RHL41 | 2465.50 | 232.00 |
13 | cluster_contig33117 | CRY1 | 371.33 | 55.86 |
TRINITY_DN46110_c0_g1_i11 | CRY1 | 90.27 | 17.33 | |
cluster_contig19944 | CRY1 | 241.05 | 52.90 | |
14 | TRINITY_DN46446_c3_g1_i8 | BIC1 | 7182.97 | 286.44 |
cluster_contig37221 | BIC1 | 2300.67 | 97.00 | |
TRINITY_DN46446_c3_g1_i6 | BIC1 | 144.56 | 10.94 | |
TRINITY_DN46446_c3_g1_i1 | BIC1 | 842.99 | 188.47 | |
cluster_contig4395 | BIC1 | 37.35 | 0.00 | |
15 | TRINITY_DN36278_c0_g1_i4 | HY5 | 534.41 | 107.30 |
TRINITY_DN36278_c0_g1_i1 | HY5 | 179.11 | 29.05 | |
16 | cluster_contig33432 | DET1 | 102.44 | 20.60 |
17 | cluster_contig20006 | UVR8 | 166.67 | 12.87 |
TRINITY_DN41117_c2_g1_i3 | UVR8 | 127.55 | 11.00 | |
TRINITY_DN41521_c1_g1_i5 | UVR8 | 1277.37 | 284.49 | |
TRINITY_DN48407_c3_g1_i15 | UVR8 | 2526.64 | 229.06 | |
cluster_contig7928 | UVR8 | 3819.14 | 796.44 | |
18 | cluster_contig31250 | LZF1 | 158.74 | 721.23 |
TRINITY_DN37789_c5_g1_i1 | LZF1 | 74.08 | 521.48 | |
TRINITY_DN47837_c0_g1_i8 | LZF1 | 7.85 | 288.19 | |
cluster_contig27900 | LZF1 | 5.16 | 202.30 | |
TRINITY_DN47837_c0_g1_i6 | LZF1 | 7.93 | 423.05 | |
cluster_contig23776 | LZF1 | 10.60 | 566.78 | |
cluster_contig20252 | LZF1 | 2.79 | 173.56 | |
TRINITY_DN47837_c0_g1_i7 | LZF1 | 0.00 | 28.78 | |
19 | cluster_contig21245 | D14 | 3.75 | 44.71 |
TRINITY_DN44313_c1_g1_i3 | D14 | 1.73 | 83.11 | |
20 | TRINITY_DN32565_c1_g2_i1 | ELIP1 | 14,497.87 | 101.38 |
TRINITY_DN32565_c1_g2_i3 | ELIP1 | 33,519.13 | 564.96 | |
21 | TRINITY_DN45074_c2_g1_i5 | LHCB5 | 827.38 | 156.19 |
22 | TRINITY_DN44026_c1_g1_i23 | PHOT2 | 112.08 | 11.49 |
TRINITY_DN44026_c1_g1_i7 | PHOT2 | 202.36 | 5.67 | |
TRINITY_DN44026_c1_g1_i1 | PHOT2 | 94.08 | 0.00 |
Heatmap Number | Gene ID | Best Homolog | FPKM (Close) | FPKM (Open) |
---|---|---|---|---|
23 | cluster_contig16162 | CslC8 | 190.47 | 26.60 |
cluster_contig34906 | CslC4 | 136.02 | 19.46 | |
24 | TRINITY_DN43633_c1_g2_i1 | CslG3 | 1335.50 | 8318.64 |
TRINITY_DN44782_c0_g1_i6 | CslG2 | 5551.84 | 684.41 | |
cluster_contig12214 | CslG2 | 538.98 | 32.21 | |
25 | TRINITY_DN40931_c0_g2_i2 | XTH-15 | 83.94 | 5911.52 |
TRINITY_DN41938_c0_g1_i4 | XTH-23 | 64.76 | 496.04 | |
TRINITY_DN43609_c2_g1_i1 | XTH-3 | 33.90 | 558.46 | |
TRINITY_DN43609_c2_g1_i8 | XTH-3 | 56.87 | 566.53 | |
TRINITY_DN47961_c4_g1_i6 | XTH-2 | 378.57 | 1794.97 | |
cluster_contig5240 | XTH-2 | 179.43 | 2105.00 | |
cluster_contig32082 | XTH-13 | 9.67 | 188.96 | |
TRINITY_DN33636_c9_g2_i3 | XTH-9 | 5.86 | 124.60 | |
26 | TRINITY_DN32754_c1_g1_i2 | EXPA10 | 280.11 | 39.51 |
TRINITY_DN32754_c2_g2_i1 | EXPA1 | 19.67 | 123.33 | |
TRINITY_DN32754_c3_g2_i2 | EXPA1 | 450.23 | 90.96 | |
27 | TRINITY_DN36767_c0_g2_i3 | CAD | 519.16 | 3871.91 |
Comparison | MgPUTs ID | RT-PCR2−ΔΔCT | p-Value | Consistency with RNAseq | Annotation (Blastx) |
---|---|---|---|---|---|
OP vs. CP | cluster_contig33312 | 16.64 ± 2.64 | 2.04 × 10−13 | Y | CBL-interacting serine/threonine-protein kinase 12 |
TRINITY_DN43916_c0_g1_i1 | 59.44 ± 5.15 | 3.90 × 10−5 | Y | Calcium-binding protein CML38 | |
TRINITY_DN38584_c2_g1_i3 | 3.00 ± 1.46 | 2.52 × 10−5 | N | Glutaredoxin-C4 | |
TRINITY_DN46984_c2_g2_i3 | 21.33 ± 2.56 | 1.85 × 10−12 | Y | Phototropin-1 | |
cluster_contig37221 | 0.41 ± 0.06 | 4.77 × 10−23 | N | Protein BIC1 | |
TRINITY_DN45074_c2_g1_i5 | 46.21 ± 0.57 | 8.58 × 10−16 | N | Light-harvesting complex II protein 5 | |
cluster_contig32082 | 1.43 ± 0.17 | 1.54 × 10−11 | Y | Putative xyloglucan endotransglucosylase/hydrolase protein 13 | |
cluster_contig15073 | 0.84 ± 0.13 | 5.22 × 10−10 | Y | Probable zinc metalloprotease EGY2 | |
TRINITY_DN44883_c0_g1_i1 | 17.00 ± 3.51 | 1.16 × 10−16 | Y | Jasmonate ZIM domain-containing protein 1 | |
cluster_contig22354 | 19.61 ± 4.01 | 3.68 × 10−11 | Y | Auxin-responsive GH3-like protein 8 | |
cluster_contig15994 | 66.56 ± 25.29 | 9.86 × 10−11 | Y | Abscisic acid 8’-hydroxylase 1 |
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Zhou, W.; Li, Z.; Wu, W.; Zhao, X.; Wang, E.; Wang, J.; Song, X.; Zhao, Y. Transcriptome Analysis Revealing the Interaction of Abscisic Acid and Cell Wall Modifications during the Flower Opening and Closing Process of Nymphaea lotus. Int. J. Mol. Sci. 2022, 23, 14524. https://doi.org/10.3390/ijms232314524
Zhou W, Li Z, Wu W, Zhao X, Wang E, Wang J, Song X, Zhao Y. Transcriptome Analysis Revealing the Interaction of Abscisic Acid and Cell Wall Modifications during the Flower Opening and Closing Process of Nymphaea lotus. International Journal of Molecular Sciences. 2022; 23(23):14524. https://doi.org/10.3390/ijms232314524
Chicago/Turabian StyleZhou, Weijuan, Zhaoji Li, Wentao Wu, Xia Zhao, Enbo Wang, Jian Wang, Xiqiang Song, and Ying Zhao. 2022. "Transcriptome Analysis Revealing the Interaction of Abscisic Acid and Cell Wall Modifications during the Flower Opening and Closing Process of Nymphaea lotus" International Journal of Molecular Sciences 23, no. 23: 14524. https://doi.org/10.3390/ijms232314524