Cyt-C Mediated Mitochondrial Pathway Plays an Important Role in Oocyte Apoptosis in Ricefield Eel (Monopterus albus)
Abstract
:1. Introduction
2. Results
2.1. Apoptosis Rates in Gonads at Different Developmental Stages
2.2. Relative Content of Apoptotic Proteins
2.3. Expression Pattern of Apoptotic Genes in the Gonads at Different Developmental Stages
2.4. Immunolocalization of Cyt-C in the Gonads
2.5. Effect of H2O2 on Gonadal Apoptosis
3. Discussion
4. Materials and Methods
4.1. Ethical Statement
4.2. Experimental Animals
4.3. Developmental Stage Identification
4.4. Apoptosis Analysis
4.5. Determination of Relative Content of Apoptotic Proteins
4.6. RNA Isolation and Quantitative RT-PCR (qRT-PCR)
4.7. Immunohistochemistry
4.8. H2O2 Incubation In Vitro
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Caspase-12 | Cyt-C | Caspase-8 | ||
---|---|---|---|---|
Apoptosis rate | Pearson correlation | −0.377 | 0.705 ** | −0.386 |
Sig. (2-tailed) | 0.063 | 0.001 | 0.056 | |
N | 25 | 25 | 25 |
Primer | Sequence (5′-3′) |
---|---|
bax F | CTTTGCCTGTCGGCTTGTCA |
bax R | ATACCCTCCCAGCCACCTTG |
apaf-1 F | TAAGAACCCCTCTGATGGCTCC |
apaf-1 R | ATTCCAAACACAGTGACCCAGC |
caspase-3 F | GCGGACTTCCTCTATGC |
caspase-3 R | CAAGGTGGCAGCAGAGT |
tnfr1 F | TCCACCTGGGGACTACGCTAC |
tnfr1 R | ACTGTCCAAGAGGGCAAGGC |
calpain F | TGAAGGGCGGAAACACCACC |
calpain R | CTCAAAGCGAGCGGGAACCA |
fadd F | GCCGACACAACGGAGTATCT |
fadd R | TTACCTCTGTGGCGATGTTC |
ef1α F | CGCTGCTGTTTCCTTCGTCC |
ef1α R | TTGCGTTCAATCTTCCATCCC |
rpl 17 F | GTTGTAGCGACGGAAAGGGAC |
rpl 17 R | GACTAAATCATGCAAGTCGAGGG |
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He, Z.; Chen, Q.; He, L.; Xiong, J.; Gao, K.; Lai, B.; Zheng, L.; Pu, Y.; Jiao, Y.; Ma, Z.; et al. Cyt-C Mediated Mitochondrial Pathway Plays an Important Role in Oocyte Apoptosis in Ricefield Eel (Monopterus albus). Int. J. Mol. Sci. 2022, 23, 10555. https://doi.org/10.3390/ijms231810555
He Z, Chen Q, He L, Xiong J, Gao K, Lai B, Zheng L, Pu Y, Jiao Y, Ma Z, et al. Cyt-C Mediated Mitochondrial Pathway Plays an Important Role in Oocyte Apoptosis in Ricefield Eel (Monopterus albus). International Journal of Molecular Sciences. 2022; 23(18):10555. https://doi.org/10.3390/ijms231810555
Chicago/Turabian StyleHe, Zhi, Qiqi Chen, Liang He, Jinxin Xiong, Kuo Gao, Bolin Lai, Li Zheng, Yong Pu, Yuanyuan Jiao, Zhijun Ma, and et al. 2022. "Cyt-C Mediated Mitochondrial Pathway Plays an Important Role in Oocyte Apoptosis in Ricefield Eel (Monopterus albus)" International Journal of Molecular Sciences 23, no. 18: 10555. https://doi.org/10.3390/ijms231810555