High Throughput Proteomic Exploration of Hypothermic Preservation Reveals Active Processes within the Cell Associated with Cold Ischemia Kinetic
Abstract
:1. Introduction
2. Results
2.1. Data Segregation
2.2. Unchanged Proteins
2.3. Proteins Affected by Anoxic Hypothermia
2.3.1. H0–H3
2.3.2. H3–H6
2.3.3. H6–H12
2.3.4. H12–H19
2.3.5. H19–H24
2.4. Protein Alteration at Rewarming/Reoxygenation
2.5. Kinetic of the Protein Expression Alteration during the Procedure
2.6. Remarkable Protein Motifs Affected by Ischemia-Reperfusion
2.6.1. Cytoskeleton Ontology
2.6.2. Transcription/Translation Ontology
- -
- Interleukin-2 enhancer-binding factor 2 (ILF2), is one of the proteins being overexpressed at the start, with the maintenance of this expression level until 12 h, upon which it is further overexpressed whereas the majority of motifs in this ontologic family are depressed. Only after 19 h do we observe the downregulation of ILF2.
- -
- Eukaryotic translation elongation factor 1 gamma (EEF1G) is a component of the translation apparatus, the eEF1 complex. We observe that its expression is constant at first then overexpressed between 6 and 12 h, before being downregulated constantly until the end of the procedure.
- -
- H2A histone family member X (H2AFX, also known as H2AX), an important member of the DNA damage detection and repair apparatus, is one of the only two motifs detected to be overexpressed between 19 and 24 h of preservation.
2.6.3. Energy Metabolism Ontology
- -
- Transketolase (TKT), a key enzyme of the pentose phosphate pathway, was upregulated between 3 and 6 h and downregulated after 12 h.
- -
- TBC1D4, a Rab GTPase-activating protein, was upregulated between 3 and 6 h and downregulated after 12 h.
- -
- Prosaposin (PSAP), a precursor to saponins, was overexpressed between 12 and 19 h.
2.6.4. Ionic Balance Ontology
- -
- Neuroblast differentiation-associated protein AHNAK, also known as desmoyokin, showed a maintained expression until 12 h, after which it is recorded as decreased both between 12 and 19 h as well as between 19 and 24 h.
- -
- Annexin A6 (ANXA6), a member of the annexins family of Ca2+ dependent phospholipid-binding proteins, was upregulated as soon as the cell was subjected to organ preservation conditions, likely related to pH alterations, and remained elevated until 12 h of preservation.
3. Discussion
4. Materials and Methods
4.1. Materials and Sample Preparation
4.2. LC-MS Parameters
4.3. Data Analysis
4.4. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
CIT | cold ischemia time |
DCD | deceased after circulatory death donors |
ECD | extended criteria donors |
HRGEC | human renal glomerular endothelial cells |
IRI | ischemia reperfusion injury |
References
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Protein ID | Protein Name | Protein Function |
---|---|---|
Peptidyl-proline modification | ||
FKBP10 | peptidyl-prolyl cis-trans isomerase FKBP10 | Accelerates the folding of proteins during synthesis. |
P4HA1 | Prolyl 4-hydroxylase subunit alpha-1 | Quaternary maturation: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. |
P4HA2 | prolyl 4-hydroxylase subunit alpha-2 | Quaternary maturation: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. |
P4HB | protein disulfide-isomerase | Catalyzes the formation, breakage, and rearrangement of disulfide bonds. |
PPIA | peptidyl-prolyl cis-trans isomerase A | Accelerates the folding of proteins during synthesis. |
PPIB | Peptidyl-prolyl cis-trans isomerase B | Accelerates the folding of proteins during synthesis. |
Peptidyl-asparagine modification | ||
CALR | Calreticulin | Calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER). |
GANAB | neutral alpha-glucosidase AB | Catalyzes the maturation of oligosaccharide precursors on proteins. |
PDIA3 | protein disulfide-isomerase A3 | Catalyzes the rearrangement of -S-S- bonds in proteins. |
UAP1 | UDP-N-acetylhexosamine pyrophosphorylase | Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. |
UGGT1 | UDP-glucose: glycoprotein glucosyltransferase 1 | Provides quality control for glycoprotein folding in the endoplasmic reticulum. |
Protein folding | ||
CALR | Calreticulin | Calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER). |
CCT4 | T-complex protein 1 subunit delta | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. |
CCT8 | T-complex protein 1 subunit theta | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. |
ERO1A | ERO1-like protein alpha | Oxidoreductase is involved in disulfide bond formation in the endoplasmic reticulum. |
FKBP10 | peptidyl-prolyl cis-trans isomerase FKBP10 | Accelerates the folding of proteins during synthesis. |
GANAB | neutral alpha-glucosidase AB | Catalyzes the maturation of oligosaccharide precursors on proteins. |
HSPA5 | 78 kDa glucose-regulated protein | Involved in the correct folding of proteins and degradation of misfolded proteins |
HSPD1 | 60 kDa heat shock protein, mitochondrial | Chaperonin is implicated in mitochondrial protein import and macromolecular assembly. |
P4HB | protein disulfide-isomerase | Catalyzes the formation, breakage, and rearrangement of disulfide bonds. |
PDIA3 | protein disulfide-isomerase A3 | Catalyzes the rearrangement of -S-S- bonds in proteins. |
PDIA6 | protein disulfide-isomerase A6 | Chaperone that inhibits aggregation of misfolded proteins. |
PPIA | peptidyl-prolyl cis-trans isomerase A | Accelerates the folding of proteins during synthesis. |
PPIB | Peptidyl-prolyl cis-trans isomerase B | Accelerates the folding of proteins during synthesis. |
TXNDC5 | Thioredoxin domain-containing protein 5 | Possesses thioredoxin activity |
UGGT1 | UDP-glucose: glycoprotein glucosyltransferase 1 | Provides quality control for glycoprotein folding in the endoplasmic reticulum. |
TLN1 | Talin-1 | Probably involved in connections of major cytoskeletal structures to the plasma membrane. |
HYOU1 | Hypoxia up-regulated protein 1 | Plays a role as a molecular chaperone and participates in protein folding. |
SERPINH1 | serpin H1 | Involved as a chaperone in the biosynthetic pathway of collagen. |
Cellular response to metal ion | ||
CALR | Calreticulin | Calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER). |
CLIC4 | Chloride intracellular channel protein 4 | Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. |
FASN | Fatty acid synthase | Catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. |
MT1G | Metallothionein-1G | Negative regulation of growth |
Glucose catabolic process | ||
ENO1 | Alpha-enolase | The multifunctional enzyme, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance, and allergic responses. |
G6PD | Glucose-6-phosphate 1-dehydrogenase | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway. |
GPI | Glucose-6-phosphate isomerase | Glycolytic enzyme. |
LDHA | L-lactate dehydrogenase A chain | Involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate |
PGD | 6-phosphogluconate dehydrogenase, decarboxylating | Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH |
PHGDH | D-3-phosphoglycerate dehydrogenase | Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. |
PKM | Pyruvate kinase PKM | The glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. |
TPI1 | Triosephosphate isomerase | Involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis |
PRDX5 | Peroxiredoxin-5, mitochondrial | NADP metabolic process |
Protein stabilization | ||
CALR | Calreticulin | Calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER). |
HSPD1 | 60 kDa heat shock protein, mitochondrial | Chaperonin is implicated in mitochondrial protein import and macromolecular assembly. |
LAMP2 | Lysosome-associated membrane glycoprotein 2 | Plays an important role in chaperone-mediated autophagy |
PPIB | Peptidyl-prolyl cis-trans isomerase B | Accelerates the folding of proteins during synthesis. |
ERO1A | ERO1-like protein alpha | Oxidoreductase is involved in disulfide bond formation in the endoplasmic reticulum. |
Microtubule-based transport | ||
COPG1 | Coatomer subunit gamma-1 | Mediates biosynthetic protein transport from the ER, via the Golgi up to the trans-Golgi network. |
LRPPRC | Leucine-rich PPR motif-containing protein, mitochondrial | May play a role in RNA metabolism in both nuclei and mitochondria. |
RAB1A | Ras-related protein Rab-1A | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. |
UCHL1 | Ubiquitin C-Terminal Hydrolase L1 | This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. |
Protein export from the nucleus | ||
CALR | Calreticulin | Calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER). |
CSE1L | Exportin-2 | Export receptor for importin-alpha. |
EIF5A | Eukaryotic translation initiation factor 5A-1 | mRNA-binding protein involved in translation elongation. |
Regulation of acute inflammatory response | ||
ANXA1 | Annexin A1 | Plays important roles in the innate immune response as an effector of glucocorticoid-mediated responses and regulator of the inflammatory process. |
C1QBP | Complement component 1 Q subcomponent-binding protein, mitochondrial | Involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation, and pre-mRNA splicing |
CD59 | CD59 glycoprotein | Potent inhibitor of the complement membrane attack complex (MAC) action. |
PCBP2 | Poly(rC)-binding protein 2 | Single-stranded nucleic acid-binding protein that binds preferentially to oligo dC. |
A: UPREGULATED | ||
Acronym | Name | Function |
Cytoskeleton | ||
CALD1 | Caldesmon 1 | Calmodulin- and actin-binding protein that plays an essential role in the regulation of actin/myosin interactions. |
Transcription/Translation Regulation | ||
ILF2 | Interleukin enhancer-binding factor 2 | Transcription factor, also involved in RNA transfer from the nucleus to the cytoplasm |
RPS2 | 40S ribosomal protein S2 | Ribosome component, small subunit |
RPS6 | 40S ribosomal protein S6 | Ribosome component, small subunit, involved in the selective translation of particular classes of mRNA |
RPS20 | 40S ribosomal protein S20 | Ribosome component, small subunit |
RPL18A | 60S ribosomal protein L18a | Ribosome component, large subunit |
RPL22 | 60S ribosomal protein L22 | Ribosome component, large subunit |
SEC61A1 | Protein transport protein Sec61 subunit alpha isoform 1 | Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. |
Ionic balance regulation | ||
ANXA6 | Annexin A6 | Regulate the release of Ca2+ from intracellular stores. |
Interaction between the cell and its environment | ||
CD151 | CD151 antigen | Cell surface protein, involved in adhesion, mobility, integrin trafficking |
B: DOWNREGULATED | ||
Acronym | Name | Function |
Energy metabolism | ||
SLC16A3 | Monocarboxylate transporter 4 | Monocarboxylate transporter 4 |
GBE1 | 1,4-alpha-glucan-branching enzyme | 1,4-alpha-glucan-branching enzyme |
Nucleotide biosynthesis | ||
PAICS | Multifunctional protein ADE2 | Multifunctional protein ADE2 |
CTPS1 | CTP synthase 1 | CTP synthase 1 |
Interaction between the cell and its environment | ||
EMP3 | Epithelial membrane protein 3 | Epithelial membrane protein 3 |
RedOx regulation | ||
GSTP1 | Glutathione S-transferase P | Glutathione S-transferase P |
A: UPREGULATED | ||
Acronym | Name | Function |
Cytoskeleton | ||
S100A6 | Protein S100-A6 | Calcium sensor and modulator. Indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. |
Transcription/Translation Regulation | ||
RPS11 | 40S ribosomal protein S11 | Ribosome component, small subunit |
RPL7 | 60S ribosomal protein L7 | Ribosome component, large subunit |
TGM2 | Protein-glutamine gamma-glutamyltransferase 2 | Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. |
Ionic balance regulation | ||
S100A6 | Protein S100-A6 | Calcium sensor and modulator. Indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. |
Energy Metabolism | ||
TKT | Transketolase | NADP metabolic process. |
TBC1D4 | TBC1 domain family member 4 | Can promote glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake |
B: DOWNREGULATED | ||
Acronym | Name | Function |
Cytoskeleton | ||
PLEC | Plectin | Interlinks intermediate filaments with microtubules and microfilaments. Anchors intermediate filaments to desmosomes or hemidesmosomes. |
Transcription/Translation Regulation | ||
RPS4X | 40S ribosomal protein S4, X isoform | Ribosome component, large subunit |
RPL22 | 60S ribosomal protein L22 | Ribosome component, large subunit |
PDIA4 | Protein disulfide-isomerase A4 | Catalyzes the rearrangement of -S-S- bonds in proteins. |
Interaction between the cell and its environment | ||
CD151 | CD151 antigen | Essential for the proper assembly of the glomerular and tubular basement membranes in the kidney. |
A: UPREGULATED | ||
Acronym | Name | Function |
Cytoskeleton | ||
MSN | Moesin | Member of the ERM family, functions as cross-linkers between plasma membranes and actin-based cytoskeletons |
ZYX | Zyxin | Mediates adhesion-stimulated changes in gene expression and modulates the organization of actin bundles. |
Transcription/Translation Regulation | ||
RPS4X | 40S ribosomal protein S4, X isoform | Ribosome component, small subunit |
RPS15 | 40S ribosomal protein S15 | Ribosome component, small subunit |
RTN4 | Reticulon-4 | Regulates membrane morphogenesis in the ER, nuclear pore complex formation, and proper localization of inner nuclear membrane proteins |
EEF1G | Elongation factor 1-gamma | Probably plays a role in anchoring the complex to other cellular components, translational elongation |
Interaction between the cell and its environment | ||
CLTC | Clathrin heavy chain 1 | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. |
ZYX | Zyxin | Mediates adhesion-stimulated changes in gene expression and modulates the organization of actin bundles. |
B: DOWNREGULATED | ||
Acronym | Name | Function |
Intracellular Transport | ||
SLC7A5 | Large neutral amino acids transporter small subunit 1 | High-affinity transport of large neutral amino acids. |
TMED2 | Transmembrane emp24 domain-containing protein 2 | Involved in vesicular protein trafficking. |
Interaction between the cell and its environment | ||
FN1 | Fibronectin | Involved in cell adhesion, cell motility, and maintenance of cell shape. |
A: UPREGULATED | ||
Acronym | Name | Function |
Energy metabolism | ||
PSAP | Prosaposin | Localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. |
PRKCSH | Glucosidase 2 subunit beta | Regulatory subunit of glucosidase II |
Transcription/Translation Regulation | ||
ILF2 | Interleukin enhancer-binding factor 2 | Transcription factor, also involved in RNA transfer from the nucleus to the cytoplasm |
Interaction between the cell and its environment | ||
ITGB1 | Integrin beta-1 | Receptors for collagen. |
Intracellular Transport | ||
SLC7A5 | Large neutral amino acids transporter small subunit 1 | High-affinity transport of large neutral amino acids. |
B: DOWNREGULATED | ||
Acronym | Name | Function |
Energy metabolism | ||
TKT | Transketolase | NADP metabolic process. |
PYGB | Glycogen phosphorylase, brain form | Glycogen phosphorylase that regulates glycogen mobilization |
Interaction between the cell and its environment | ||
ANXA2 | Annexin A2 | Regulates membrane budding, membrane raft assembly |
ZYX | Zyxin | Adhesion plaque protein. |
FERMT2 | Fermitin family homolog 2 | Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. |
Cytoskeleton | ||
TAGLN2 | Transgelin 2 | Actin filament binding, the possible regulatory role |
MSN | Moesin | Member of the ERM family, functions as cross-linkers between plasma membranes and actin-based cytoskeletons |
S100A11 | S100 Calcium Binding Protein A11 | This protein may function in motility, invasion, and tubulin polymerization. |
CAP1 | Adenylyl cyclase-associated protein 1 | Directly regulates filament dynamics, implicated in mRNA localization and the establishment of cell polarity. |
IQGAP1 | Ras GTPase-activating-like protein IQGAP1 | Serves as an assembly scaffold for the organization of the actin cytoskeleton at the plasma membrane. |
FSCN1 | Fascin Actin-Bundling Protein 1 | Fascin proteins organize F-actin into parallel bundles and are required for the formation of actin-based cellular protrusions. |
Ionic balance regulation | ||
AHNAK | AHNAK Nucleoprotein | May be required for neuronal cell differentiation. regulation of RNA splicing |
Transcription/Translation Regulation | ||
EEF1A1P5 | Eukaryotic Translation Elongation Factor 1 Alpha 1 Pseudogene 5 | Translation elongation factor |
EEF1G | Elongation factor 1-gamma | Translation elongation factor |
RPS13 | 40S ribosomal protein S13 | Ribosome component, small subunit |
RPS15 | 40S ribosomal protein S15 | Ribosome component, small subunit |
RPS24 | 40S ribosomal protein S24 | Ribosome component, small subunit |
RPL18 | 60S ribosomal protein L18 | Ribosome component, large subunit |
RPL13 | 60S ribosomal protein L13 | Ribosome component, large subunit |
SARS | Serine--tRNA ligase, cytoplasmic | Catalyzes the attachment of serine to tRNA(Ser). |
PABPC4 | Polyadenylate-binding protein 4 | May be involved in cytoplasmic regulatory processes of mRNA metabolism. |
Intracellular Transport | ||
ARF4 | ADP Ribosylation Factor 4 | Plays a role in vesicular trafficking and as an activator of phospholipase D |
DYNC1H1 | Cytoplasmic dynein 1 heavy chain 1 | Acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
RedOx regulation | ||
PRDX6 | Peroxiredoxin-6 | Involved in redox regulation of the cell |
A: UPREGULATED | ||
Acronym | Name | Function |
Cytoskeleton | ||
S100A6 | Protein S100-A6 | Calcium sensor and modulator. Indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. |
Transcription/Translation Regulation | ||
H2AFX | H2A Histone Family Member X | Variant histone H2A that replaces conventional H2A in a subset of nucleosomes. |
Ionic balance regulation | ||
S100A6 | Protein S100-A6 | Calcium sensor and modulator. Indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. |
B: DOWNREGULATED | ||
Acronym | Name | Function |
Energy metabolism | ||
TBC1D4 | TBC1 domain family member 4 | Can promote glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake |
Cytoskeleton | ||
SPTBN1 | Spectrin beta chain, non-erythrocytic 1 | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton. |
PDLIM7 | PDZ and LIM domain protein 7 | May function as a scaffold on which the coordinated assembly of proteins can occur. |
SNU13 | Spectrin alpha chain, non-erythrocytic 1 | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton. |
Transcription/Translation Regulation | ||
EEF1G | Elongation factor 1-gamma | Translation elongation factor |
RPS2 | 40S ribosomal protein S2 | Ribosome component, small subunit |
RPS11 | 40S ribosomal protein S11 | Ribosome component, small subunit |
RPL7 | 60S ribosomal protein L7 | Ribosome component, large subunit |
ILF2 | Interleukin enhancer-binding factor 2 | Transcription factor, also involved in RNA transfer from the nucleus to the cytoplasm |
Proteasome | ||
PSMD3 | 26S proteasome non-ATPase regulatory subunit 3 | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. |
A: UPREGULATED | ||
None. | ||
B: DOWNREGULATED | ||
Acronym | Name | Function |
Energy metabolism | ||
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide. |
ALDOA | Fructose-bisphosphate aldolase A | Plays a key role in glycolysis and gluconeogenesis. |
Interaction between the cell and its environment | ||
FN1 | Fibronectin | Involved in cell adhesion, cell motility, and maintenance of cell shape. |
CLTC | Clathrin heavy chain 1 | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. |
Cytoskeleton | ||
MYH9 | Myosin-9 | Plays a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation, and lamellipodial retraction; this function is mechanically antagonized by MYH10 |
FLNA | Filamin-A | Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins |
CFL1 | Cofilin-1 | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. |
SPTAN1 | Spectrin beta chain, non-erythrocytic 1 | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton. |
SPTBN1 | Spectrin beta chain, non-erythrocytic 1 | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton. |
Ionic balance regulation | ||
AHNAK | AHNAK Nucleoprotein | May be required for neuronal cell differentiation. regulation of RNA splicing |
Transcription/Translation Regulation | ||
EEF1A1P5 | Eukaryotic Translation Elongation Factor 1 Alpha 1 Pseudogene 5 | Translation elongation factor |
HNRNPA2B1 | Heterogeneous nuclear ribonucleoproteins A2/B1 | Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles |
HNRNPD | Heterogeneous nuclear ribonucleoprotein D0 | Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles |
IPO5 | Importin-5 | Functions in nuclear protein import as nuclear transport receptor. |
PTRF | Caveolae Associated Protein 1 | Enables the dissociation of paused ternary polymerase I transcription complexes from the 3’ end of pre-rRNA transcripts. |
PTBP1 | Polypyrimidine tract-binding protein 1 | Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. |
RPS2 | 40S ribosomal protein S2 | Ribosome component, small subunit |
RPS4X | 40S ribosomal protein S4, X isoform | Ribosome component, small subunit |
RPS5 | 40S ribosomal protein S5 | Ribosome component, small subunit |
RPS8 | 40S ribosomal protein S8 | Ribosome component, small subunit |
RPS13 | 40S ribosomal protein S13 | Ribosome component, small subunit |
RPL3 | 60S ribosomal protein L3 | Ribosome component, large subunit |
RPL4 | 60S ribosomal protein L4 | Ribosome component, large subunit |
RPL6 | 60S ribosomal protein L6 | Ribosome component, large subunit |
RPL7 | 60S ribosomal protein L7 | Ribosome component, large subunit |
RPL13 | 60S ribosomal protein L13 | Ribosome component, large subunit |
RPL18 | 60S ribosomal protein L18 | Ribosome component, large subunit, involved in translational initiation |
RPL18A | 60S ribosomal protein L18a | Ribosome component, large subunit |
RPL23 | 60S ribosomal protein L23 | Ribosome component, large subunit, possesses ubiquitin ligase inhibitor activity |
RPL28 | 60S ribosomal protein L28 | Ribosome component, large subunit, positive regulation of protein targeting to the mitochondrion, regulation of mitophagy |
SSB | Ribosome-associated molecular chaperone SSB1 | Ribosome-bound, Hsp70-type chaperone that assists in the cotranslational folding of newly synthesized proteins in the cytosol. |
HSPB1 | Heat shock protein beta-1 | Functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state |
H2AFX | H2A Histone Family Member X | Variant histone H2A replaces conventional H2A in a subset of nucleosomes. |
ILF2 | Interleukin enhancer-binding factor 2 | may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. |
PABPC4 | Polyadenylate-binding protein 4 | May be involved in cytoplasmic regulatory processes of mRNA metabolism. |
SND1 | Staphylococcal nuclease domain-containing protein 1 | Functions as a bridging factor between STAT6 and the basal transcription factor. |
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Pasini-Chabot, O.; Vincent, J.; Le Pape, S.; Lepoittevin, M.; Kaaki, W.; Woillard, J.-B.; Giraud, S.; Bourmeyster, N.; Hauet, T.; Thuillier, R. High Throughput Proteomic Exploration of Hypothermic Preservation Reveals Active Processes within the Cell Associated with Cold Ischemia Kinetic. Int. J. Mol. Sci. 2021, 22, 2384. https://doi.org/10.3390/ijms22052384
Pasini-Chabot O, Vincent J, Le Pape S, Lepoittevin M, Kaaki W, Woillard J-B, Giraud S, Bourmeyster N, Hauet T, Thuillier R. High Throughput Proteomic Exploration of Hypothermic Preservation Reveals Active Processes within the Cell Associated with Cold Ischemia Kinetic. International Journal of Molecular Sciences. 2021; 22(5):2384. https://doi.org/10.3390/ijms22052384
Chicago/Turabian StylePasini-Chabot, Ophélie, Julia Vincent, Sylvain Le Pape, Maryne Lepoittevin, Wassim Kaaki, Jean-Baptiste Woillard, Sebastien Giraud, Nicolas Bourmeyster, Thierry Hauet, and Raphael Thuillier. 2021. "High Throughput Proteomic Exploration of Hypothermic Preservation Reveals Active Processes within the Cell Associated with Cold Ischemia Kinetic" International Journal of Molecular Sciences 22, no. 5: 2384. https://doi.org/10.3390/ijms22052384