Increasing Transfection Efficiency of Lipoplexes by Modulating Complexation Solution for Transient Gene Expression
Abstract
:1. Introduction
2. Results and Discussion
2.1. Lipoplex Preparation with Cationic Lipids and pDNA
2.2. Effects of Complexation Solution on Transfection
2.3. Characteristics of Liposomes, Lipoplexes, and pDNA
2.4. Cellular Uptake and Decomplexation of the Lipoplex
3. Materials and Methods
3.1. Cell Culture
3.2. Transfection Reagents and Expression Vectors
3.3. Transfection
3.4. Analysis of Protein Titer and GFP-Positive Cells
3.5. Dynamic Light Scattering
3.6. Gel Electrophoresis
3.7. Fluorescence Imaging
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, J.; Kim, J.Y.; Kim, H.; Kim, E.; Park, S.; Ryu, K.-H.; Lee, E.G. Increasing Transfection Efficiency of Lipoplexes by Modulating Complexation Solution for Transient Gene Expression. Int. J. Mol. Sci. 2021, 22, 12344. https://doi.org/10.3390/ijms222212344
Kim J, Kim JY, Kim H, Kim E, Park S, Ryu K-H, Lee EG. Increasing Transfection Efficiency of Lipoplexes by Modulating Complexation Solution for Transient Gene Expression. International Journal of Molecular Sciences. 2021; 22(22):12344. https://doi.org/10.3390/ijms222212344
Chicago/Turabian StyleKim, Jaemun, Ji Yul Kim, Hyeonkyeong Kim, Eunsil Kim, Soonyong Park, Kyoung-Hwa Ryu, and Eun Gyo Lee. 2021. "Increasing Transfection Efficiency of Lipoplexes by Modulating Complexation Solution for Transient Gene Expression" International Journal of Molecular Sciences 22, no. 22: 12344. https://doi.org/10.3390/ijms222212344