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Review

Transcriptional Control of Apical-Basal Polarity Regulators

1
Department of Molecular Cell Physiology, Institute of Physiology and Pathophysiology, Philipps-University, 35037 Marburg, Germany
2
Department of Molecular Cell Biology, Institute I for Anatomy, Faculty of Medicine and University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany
3
Cluster of Excellence—Cellular Stress Response in Aging-Associated Diseases (CECAD), University of Cologne, Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany
4
Center for Molecular Medicine Cologne, Faculty of Medicine and University Hospital Cologne, University of Cologne, Robert-Koch-Str. 21, 50931 Cologne, Germany
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(22), 12340; https://doi.org/10.3390/ijms222212340
Submission received: 15 October 2021 / Revised: 6 November 2021 / Accepted: 10 November 2021 / Published: 15 November 2021

Abstract

:
Cell polarity is essential for many functions of cells and tissues including the initial establishment and subsequent maintenance of epithelial tissues, asymmetric cell division, and morphogenetic movements. Cell polarity along the apical-basal axis is controlled by three protein complexes that interact with and co-regulate each other: The Par-, Crumbs-, and Scrib-complexes. The localization and activity of the components of these complexes is predominantly controlled by protein-protein interactions and protein phosphorylation status. Increasing evidence accumulates that, besides the regulation at the protein level, the precise expression control of polarity determinants contributes substantially to cell polarity regulation. Here we review how gene expression regulation influences processes that depend on the induction, maintenance, or abolishment of cell polarity with a special focus on epithelial to mesenchymal transition and asymmetric stem cell division. We conclude that gene expression control is an important and often neglected mechanism in the control of cell polarity.

1. Introduction

Cell polarity refers to the subcellular asymmetry of the plasma membrane, cytoskeleton, or cellular organelles and is vital for the function of a broad range of cell types [1,2,3,4,5,6,7,8,9]. Cell types that rely on cell polarity range from epithelial cells to asymmetrically dividing cells and include neurons, migrating cells, and zygotes. The regulation of cell polarity is of utmost importance for cellular function and cells employ a variety of mechanisms to ensure appropriate abundance and activity of polarity determinants. Localization and activity of polarity proteins is heavily regulated through kinases and phosphatases [10,11,12]. In addition, increasing evidence suggests that protein stability via proteasomal degradation contributes to polarity control [13]. While the regulation of cell polarity at the protein level is fairly well understood, it is less well understood how the expression of genes encoding polarity proteins is regulated. Research has identified a number of processes in which polarity genes are transcriptionally controlled [14,15]. Here we review the recent literature on how polarity gene expression coordinates cell polarity with particular focus on epithelial cells and asymmetrically dividing stem cells across species and identify common themes.
Epithelial cells exhibit apical-basal polarization with an outwards-facing apical membrane domain and a basal side that faces the extracellular matrix of the basal lamina. The lateral domains of epithelial cells are characterized by intercellular junctions that mediate cell-cell adhesion. These features are essential for normal tissue morphogenesis and function [16]. While the formation of epithelial tissues requires the establishment of cell polarity, the production of mesodermal tissues, through epithelial to mesenchymal transition (EMT), relies on the loss of cell polarity [17]. Furthermore, cell polarity is tightly connected to proliferation and cellular growth. Correspondingly, many cell polarity determinants have tumor suppressive or pro-oncogenic properties and have been reported to be mis-regulated in a variety of different tumors, particularly in those of epithelial origin [18,19].
Similar to how the mis-regulation of cell polarity in epithelial cells can promote tumorigenesis, the loss of cell polarity in asymmetrically dividing cells can have adverse consequences. During asymmetric cell division, cell polarity assumes a dual function in the orientation of the spindle apparatus as well as the localization of cell fate determinants along the division axis, which results in the subsequent asymmetric inheritance of cell fate determinants by the resulting daughter cells. Asymmetric cell division is a common motif in stem cell division and faulty cell polarity can result in stem cell loss or over-proliferation and confers a susceptibility to tumorigenesis [20,21].
The establishment and maintenance of cell polarity is particularly well characterized in epithelial tissues and the protein determinants involved are highly conserved among species (Table 1).
Epithelial polarity is controlled by three protein complexes that interact and cross-regulate each other (Figure 1A): the apically localized Partitioning defective (Par) and Crumbs (Crb) complexes, and the Scribble (Scrib) complex, which localizes basolaterally [24,25]. The Par complex consists of the atypical protein kinase C (aPKC), whose activity is controlled by its interactor Par-6 and the small GTPase cell division control protein 42 (Cdc42). Together with the scaffold proteins Par-3/Bazooka (Baz), Cdc42 also contributes to the localization of the aPKC-Par-6 complex [26]. The Par complex exerts a major function in regulating the phosphorylation status of polarity determinants, including components of the Par complex itself and of additional targets whose localization and/or activity is influenced by phosphorylation. The Scrib complex consists of the scaffold proteins Scribble (Scrib), Lethal giant larvae (Lgl), and Discs large (Dlg), all of which are composed of multiple protein-protein interaction domains. Lgl is excluded from the apical cortex through phosphorylation by aPKC. In turn, Lgl binds to the Par complex to inhibit aPKC, thereby rendering the kinase inactive in the basolateral cortex [27,28,29]. Like the Scrib complex, the Crb complex exerts its function via the regulation and facilitation of protein-protein interactions. The Crb complex contains the transmembrane protein Crb, which interacts with the scaffold protein Stardust (Sdt)/PALS1 via its intracellular domain, which in turn binds to PATJ and Lin-7. Members of the Crb complex can also form transient interactions with Par complex components [30]. For example, after aPKC-Par-6 are recruited via Baz/Par-3, the complex is handed over to Crb, which contributes to correct localization of aPKC-Par-6 [31].
Asymmetric stem cell division is often investigated using the Drosophila neuroblast model (Figure 1B). In contrast to their mutually exclusive localization in epithelia, the Par and Scrib complexes co-localize apically in neuroblasts, and phosphorylation of Dlg1 by aPKC disrupts Dlg1 autoinhibition, which then allows it to interact with the spindle orientation factor GukHolder [32]. In neuroblasts, the phosphatase PP2A dephosphorylates both Par-6 and Baz, which leads to reduced aPKC kinase activity [33,34]. However, the interaction of PP2A with the Par complex is not restricted to Drosophila neuroblasts. In epithelial cells PP2A also regulates aPKC, thereby functioning in a similar fashion [11,35].
Another important player in asymmetric cell division is the adaptor protein Inscuteable (Insc), which is required for apical localization of the Par complex and orients the spindle apparatus by interaction with the microtubule binding protein Mushroom body defective (Mud) and the adaptor protein Partner of Inscuteable (Pins) [26].
It is important to note that many polarity determinants also possess functions outside of polarity protein complexes. For example, outside of the Par complex, Baz interacts with the adherens junction (AJ) core component E-cadherin. In humans, two distinct Par-3 homologs function at AJs (PARD3B) and in the Par complex (PARD3) [36]. In addition, besides regulation of apical-basal polarity, aPKC is part of several additional signaling pathways and Scrib is an important regulator of planar cell polarity [37,38].

2. Polarity Gene Expression during Epithelial to Mesenchymal Transition

When epithelial cells become mesenchymal during EMT, the loss of cell polarity is a crucial prerequisite for this process [39]. Hence, the expression of polarity proteins has to be repressed permanently. Importantly, because EMT is one of the hallmarks of cancer progression, understanding the steps that result in EMT is of high clinical relevance [40].
The regulation of the cell adhesion molecule E-Cadherin is the most well characterized example of transcriptional regulation during EMT and has been reviewed in great detail in the past [23,41,42,43]. In short, the expression of the E-Cadherin encoding gene is directly regulated by a plethora of transcription factors (see Table 1), out of which the E-box binding factors SNAIL, SLUG, ZEB1, ZEB2, and Twist1/2 repress transcription and RUNX1, FOXA, p300, Rb, c-Myc, and AP-2 contribute to activation of transcription. Further, the chromatin regulators PRC2, G9a, and LSD1 as well as the Jak-Stat signaling pathway contribute to E-cadherin gene expression. During EMT, E-Cadherin downregulation is often accompanied by an upregulation of N-Cadherin, which is transcriptionally regulated by NFκB [44,45]. E-Cadherin and N-Cadherin expression is tightly linked. First, E-Cadherin represses NFκB via p38 MAPK, second NFκB induces the expression of the genes encoding the EMT-inducing transcription factors SNAIL, SLUG, ZEB2 and TWIST1 [45,46].
Several transcription factors regulating E-Cadherin transcription also target polarity protein expression and a few transcription factors that have not been described to regulate E-Cadherin transcription further influence EMT via the regulation of cell polarity (Table 1). Among these, the zinc finger transcription factor Snail has the biggest known repertoire of targets among polarity genes in different tissues and species [47]. Like its mammalian homolog, Drosophila Snail is competent to induce EMT-driven tumors, suggesting that studies conducted in model organisms are highly relevant [48]. Among the polarity regulators regulated by Snail, not all targets are directly regulated. For example, during Drosophila gastrulation, Snail represses Baz on the post-transcriptional level [49]. This Snail-mediated downregulation targets the junctional function of Baz, as it results in a decrease of E-cadherin at AJs without affecting other E-cadherin pools. Hence, this regulation of Baz contributes to the regulation of E-cadherin rather than the regulation of cell polarity complexes. The Snail-mediated repression of Baz is highly dynamic. During mesoderm internalization, when AJs shift apically in order to allow tissue folding, Baz repression is transiently blocked. While the authors did not test the exact mode of how the Snail represses Baz, several facts suggest that it may not take place at the level of transcription. First, baz mRNA is mainly maternally supplied during this stage of Drosophila embryogenesis. Second, when baz is expressed under the control of heterologous promoters, the protein is still removed from junctional sites and lastly, the regulation is highly dynamic in nature, further arguing for a regulation at the post-transcriptional level [49].
In contrast, the gene encoding human DLG1 is known to be directly bound by SNAIL. The regulatory region of the gene encoding this member of the Scrib complex contains several SNAIL consensus-binding E-box sites and is directly repressed by SNAIL during cancer progression of a variety of tumor types [50]. Thus, it is likely that SNAIL regulates DLG1 during developmental EMT as well. Similarly, the human gene encoding the Lgl homolog LLGL2/Hugl-2 contains E-box sequences and is directly bound and repressed by SNAIL in breast cancer cells [51]. This repression of LLGL2 is instrumental for SNAIL -mediated EMT, as the removal of the LLGL2 E-box sites reverses the SNAIL -induced phenotype.
SNAIL does not only target the Scrib complex, but has a repressive effect on the Crb complex as well [52]. In Madin–Darby Canine Kidney (MDCK) cells, SNAIL represses transcription of the CRB3 gene and also results in reduced transcription of the Crb complex components PATJ and PALS1. In contrast, Par and Scrib complex component levels were largely unaffected. The CRB3 gene contains several E-box sequences, which are directly bound by SNAIL. Interestingly, SNAIL levels did not always correlate with the level of CRB3 transcriptional downregulation, hence additional mechanisms must contribute to CRB3 transcriptional regulation [52]. DamID experiments in Drosophila also revealed binding of Snail to the crb locus, consistent with crb downregulation during neuroblast selection, which is an EMT-like process [53].
E-box sequences can be bound by other transcription factors, including ZEB-1, which has been shown to repress LLGL2, PATJ, and CRB3 in a breast cancer cell line [54]. Whiteman et al. [52] speculate that while SNAIL mediates the initial reduction of CRB3 expression during EMT, other E-box binding transcription factors such as ZEB-1, ZEB-2, SLUG, and E47 are responsible for sustained CRB3 repression. Another study conducted in breast cancer cells further supports the presence of SNAIL-independent regulatory mechanisms of CRB3 expression. In this study, while SNAIL binding to the CRB3 promoter was equally observed, this did not result in a relevant downregulation of CRB3 transcript levels [55]. Instead, the transcription factor ZEB-1 associates with MUC1-C to directly repress CRB3. Furthermore, CRB3 is regulated by the transcription factor estrogen receptor α (ERα) in breast cancer cells. However, this regulation takes place post-transcriptionally and most likely occurs at the level of protein stability [56].
Human CRB2, but not CRB3, is repressed by the transcription factor hGATA6, named after its ability to bind to “GATA” DNA sequences. This regulatory mechanism was first discovered during Drosophila EMT, where the hGATA6 homolog Srp represses crb directly [57]. Srp also targets genes encoding other polarized proteins including the Crb complex member Sdt, the apically localized Stranded-at-second (Sas) and the basolateral Claudins Sinuous (Sinu), Megatrachea (Mega), and Kune-kune (Kune). However, the Par complex proteins Baz, aPKC and Par-6 are not affected by Drosophila Srp and the CRB interactors LIN-7 and PATJ are not targeted by human hGATA6 [57].

3. Polarity Gene Expression and the Regulation of Asymmetric Stem Cell Division

Asymmetric cell division allows stem cells to reproduce a stem cell, which inherits the stem cell specific factors, while differentiation factors are loaded into the second, differentiating the daughter cell. This mechanism promotes the rapid differentiation of the non-stem cell daughter and contributes to stem cell maintenance. In addition to the asymmetric inheritance of cell fate determinants, the continued expression of stem cell fate determinants has to be ensured in the mother cell. At the same time, it is sensible to employ mechanisms to repress the transcription of stem cell factors in differentiating daughter cells.
One such mechanism in which a stem cell factor is transcriptionally regulated during asymmetric stem cell division has been described in Drosophila neuroblasts. The Par complex kinase aPKC promotes neuroblast self-renewal and is apically localized during division and thus inherited by the neuroblast daughter. In the neuroblast, aPKC participates in a feedback mechanism resulting in its transcriptional regulation [58]. aPKC phosphorylates the transcription factor Zinc-finger protein (Zif), which prevents Zif nuclear entry. Upon neuroblast division, the basally formed ganglion mother cell accumulates unphosphorylated Zif, which enters the nucleus to directly repress aPKC transcription. This promotes differentiation and blocks reversion to a stem cell-like state. Interestingly, neuroblasts display nuclear Zif localization despite the aPKC-mediated nuclear exclusion of Zif, suggesting that not the entire Zif protein pool is phosphorylated in neuroblasts. In agreement, zif mutation does not only lead to a failure in daughter cell differentiation but also results in phenotypes in the neuroblast itself: zif mutant neuroblasts display mislocalization of polarity determinants, which is mostly rescued in an aPKC heterozygous mutant background. Hence, while Zif functions to repress aPKC in ganglion mother cells to allow differentiation, it fine tunes aPKC levels in neuroblasts to regulate cell polarity [58].
In the larval neuroblast, the continued expression of aPKC is ensured by the transcription factor Myc, which is well known for its roles in cell cycle progression and positive regulation of cell growth [59]. Myc binds to the aPKC gene and recruits the Tip60 chromatin remodeler complex, which increases the permissive euchromatin marks H4K8Ac and H2Av and induces aPKC expression. Knockdown of components of the Myc-Tip60 complex or aPKC led to loss of apical-basal polarity. Neuroblasts were smaller and divided symmetrically and were ultimately lost by premature differentiation via nuclear entry of the aPKC target and transcription factor Prospero. Interestingly, restoration of aPKC levels restored apical-basal polarity in Myc-Tip60 complex knockdown but failed to rescue asymmetric division and Prospero nuclear entry. The persistence of symmetric divisions in Myc-Tip60 knockdown rescued with aPKC overexpression can be traced to several Myc-Tip60 targets regulating the spindle and centrosomes while the premature nuclear entry of Prospero is likely connected to the impact of Myc-Tip60 on cell size. Together, Myc and the Tip60 complex regulate not only apical-basal polarity in neuroblasts but are vital for neuroblast growth and asymmetric division [59].
The interaction between Myc and the Tip60 complex is conserved in human embryonic stem cells (ESCs) [60,61] and polarity genes are expressed in these cells, although their role is poorly understood [62,63]. Interestingly, MYC controls the balance between symmetric and asymmetric cell division in human neuroblastoma, further supporting a conserved function in cell polarity control [64].
When differentiation towards the mesendoderm is induced in mouse ESCs, these stem cells switch between symmetric and asymmetric division to balance self-renewal and differentiation. Asymmetric division is regulated by INSC, which interacts with PAR-3 and LGN/PINS. INSC orients the spindle apparatus, similar to the function of Insc in Drosophila neuroblasts. INSC levels determine whether ESCs divide symmetrically or asymmetrically, whereby high INSC levels induce asymmetric division. Hereby, the NF-κB-familiy transcription factor reticuloendotheliosis oncogene (c-Rel) binds to the INSC promoter and induces INSC transcription. This leads to increased rates of asymmetric division, which ultimately promotes mesodermal cell fates [65].
In Drosophila embryonic neuroblasts, the Snail-family transcription factors Escargot (Esg), Snail and Worniu (Wor) indirectly regulate insc expression [66,67]. These studies depict a prime example illustrating how vital it is to consider potential indirect mechanisms when studying transcription factors: Careful dissection into the mechanism revealed that both transcription as well as translation of insc are indirectly regulated by the transcription factor triad. First, Snail binding to its co-repressor C-terminal binding protein (CtBP) is crucial for Snail-mediated neuroblast specification. Yet, insc transcript levels are positively regulated by Snail, Esg, and Wor and hence insc transcription is likely indirectly induced [67]. While Esg, Snail, and Wor regulate insc transcription during early stages of neurogenesis, insc transcription is further regulated by an unknown additional mechanism during the later stages, as insc mRNA can be detected in an esg, snail, wor triple mutant in a delayed manner [66]. However, this transcriptional induction is insufficient to restore Insc protein levels in the esg, snail, wor mutant background. Thus, in addition to the transcriptional regulation, Esg, Snail, and Wor regulate insc translation, which requires the 5′ and/or 3′-UTRs of the insc mRNA. The nuclear localized Esg, Snail, and Wor proteins are unlikely to directly cause this translational regulation and instead most likely regulate insc translation via other genes. Thus, both the transcriptional as well as translational regulation of insc by Esg, Snail, and Wor are indirectly mediated. Moreover, while esg, snail, wor triple mutant neuroblasts display completely randomized spindle orientation, insc deficient neuroblasts display normal spindle positioning during telophase [66]. This so-called “telophase rescue” depends on Dlg1 and Kinesin heavy chain 73 (Khc73) [66,68,69]. The absence of this telophase rescue in dlg and khc73 mutants suggests that Dlg1 and/or Khc73 could be targets of these Snail family transcription factors in neuroblasts.

4. Polarity Gene Expression in Other Processes

Besides EMT and asymmetric stem cell division, several other processes require a fine regulation of cell polarity. These include establishment of cell-cell contacts, cell cycle progression, and differentiation [70,71]. In humans, DLG1 is one of the genes encoding polarity proteins targeted for expression control during a number of these processes. Depending on the process, DLG1 expression is regulated at the transcriptional level alone or in combination with translational efficiency via the expression of splice variants. Alternative splicing of DLG1 mRNA results in either a large or a short isoform, which encode for the same protein but differ in the 5′UTR [71]. The longer isoform is translated with lower efficiency than the shorter isoform, which is likely due to more stable secondary RNA structures of the longer isoform. Depending on the required levels of DLG1 protein, the long and short isoforms are expressed in specific ratios that allow for the fine tuning of DLG1 protein levels [70]. The factors regulating transcription and alternative splicing of DLG1 mRNA are unknown, with the exception of repression through the transcription factor Snail during EMT, as mentioned above [50].
Studies conducted in several model organisms provide further insight into the mechanisms of transcriptional control of Dlg. During C. elegans epithelium formation, dlg-1 transcription is induced by PHA-4 [72]. PHA-4 acts as a pioneer transcription factor and also induces the expression of other epithelial genes including par-3. In contrast, par-6 mRNA is maternally deposited and the zygotic transcriptional control of par-6 does not play a role during epithelial formation in C. elegans [72,73,74,75]. Upon transcription of dlg-1, the kinesin ZEN-4 interacts with its binding partner CYK-4 to regulate DLG-1 protein accumulation, as well as the accumulation of other polarity proteins including PAR-6, PKC-3/aPKC, and PAR-3 [72,76]. Whether ZEN-4 and CYK-4 regulate translation or protein stability of these cell polarity determinants is currently unknown.
During the development of the Drosophila wing disc epithelium, dlg1 is transcriptionally regulated. Wing disc development is regulated by the Dpp signaling pathway, which in turn induces the transcription of the Zinc finger transcription factors Spalt major (Salm) and Spalt-related (Salr). Salm and Salr appear to activate the expression of Dlg1 as well as its interactor Scrib [77]. In Drosophila neurons, it is clear that translational regulation contributes to Dlg1 protein expression levels. Here, the mRNA-binding protein Syncrip regulates Dlg1 protein levels and localized mRNA translation in order to regulate synaptic growth [78]. Besides, Par-3, sdt, and crb mRNAs have been shown to display distinct subcellular localizations in neurons (Par-3) or epithelial cells (sdt, crb), suggesting that local translation contributes to the regulation of cell polarity [79,80,81].
The expression of several Par complex members was shown to be regulated in a variety of tissues. In the developing Drosophila wing, aPKC protein levels are increased via Hedgehog signaling in a positive feedback loop [82]. Together with Par-6, aPKC contributes to the activation of Hedgehog signaling via the phosphorylation of Smoothened. In turn, aPKC protein levels increase in a cubitus interruptus (ci) dependent manner. Whether or not aPKC expression is regulated at the transcriptional or post-transcriptional level and whether this is a direct regulation or a secondary effect is unclear, but since ci encodes a transcription factor, a direct transcriptional regulation is conceivable. Importantly, besides Par-6, no other polarity determinants contribute to aPKC-mediated Hedgehog activation, suggesting that the regulation of Hedgehog signaling is not directly linked to cell polarity. Significantly, the role of aPKC in Hedgehog signaling activation is conserved in mammals [83] and studies in avian species show that Hedgehog signaling regulates cell polarity during neural tube formation [84]. A post-transcriptional mode of ci-mediated regulation of aPKC might represent a possibility for a targeted regulation of aPKC functions in Hedgehog signaling. Yet, transcriptional regulation of aPKC may contribute to the regulation of both the cell polarity- as well as the Hedgehog-mediating functions of aPKC.
In the Drosophila eye, aPKC inhibits the planar cell polarity (PCP) pathway via the phosphorylation of Frizzled1 (Fz1) upon its recruitment by Patj [85]. In turn, PCP signaling results in the downregulation of aPKC and Patj protein levels, while Baz protein levels are upregulated. Baz has been shown to repress aPKC kinase activity and, consistently, represses aPKC-mediated phosphorylation of Fz1. Presently, it is unclear whether the differential regulation of these polarity determinants occurs at the transcriptional or post-transcriptional level.
During heart development, a mechanism that is conserved from Drosophila to mammals, aids in the regulation of cdc42. The transcription factor Tinman in Drosophila or its mouse homolog Nkx2-5 positively regulate cdc42 levels. In mammals, Nkx2-5 represses the micro-RNA miR-1, which in turn negatively regulates CDC42 [86]. While the function of the Par complex in mammalian cardiomyocytes has not been determined yet and CDC42 has several functions outside of polarity regulation, CDC42 is important for cell-cell adhesion during heart development [87]. This suggests that the Tinman/Nkx2-5 mediated regulation of cdc42 could contribute to the regulation of Par complex function.
Drosophila spiracle development requires extensive cell shape changes that are associated with the differential regulation of cell polarity regulators as well as cytoskeletal changes [88,89]. Spiracle development is induced by the Hox transcription factor Abd-B, which induces the expression the genes encoding the transcription factor Cut and the Jak-Stat ligand Upd. In turn, the Jak-Stat effector Stat92E directly induces crb expression. However, restoration of Crb levels in a Jak-Stat deficient background cannot rescue the Jak-Stat induced phenotype. This might be due to other crucial targets of Stat92E in spiracle development such as shg, encoding Drosophila E-Cadherin, which is further controlled through Cut. Here, it should be noted that it was not tested whether the shg gene is indeed bound by Cut or Stat92E or if either could regulate shg expression indirectly [88].
While the phosphatase PP2A is not a member of the Par complex, it is important for regulating its activity [33,34,35,90]. PP2A consists of the scaffold subunit A, which interacts with Baz in Drosophila [33], a regulatory B subunit, and the catalytic C subunit, which confers the serine/threonine phosphatase activity. In mammals, several versions of each subunit exist that are encoded by distinct genes (Table 1). Four families of B subunit versions dictate target specificity. The A and C subunits each are encoded by an A and a B gene. The genes PPP2CA and PPP2CB encoding the scaffold subunit PP2A-A are differentially regulated, with a manifold higher expression of the PPP2CA gene on both the transcript as well as the protein levels. Mis-regulation of PP2A-A has been implicated in several diseases [91]. Correspondingly, the expression of both PPP2CA and PPP2CB genes have been reported to be tightly regulated by a variety of transcription factors. The PPP2CA gene is positively regulated by the transcription factors CREB, ETS-1 and AP-2α and negatively regulated by SP-1, all of which have been reported to directly bind to the PPP2CA promoter [92]. The PPP2CB gene is positively regulated by SP1/SP3 and RXRα/β and negatively regulated by ETS-1 through direct interaction [93]. The functional implications of these extensive regulatory mechanisms have not been described and PP2A targets many proteins outside of the Par complex. For example, it regulates a number of junctional proteins and plays an important role in cell cycle regulation [11,94]. Yet, these transcription factors should be considered candidates for potential regulators of cell polarity determinants.

5. Discussion

Among the various processes requiring differential regulation of cell polarity determinants, the regulation of EMT is particularly well understood. Strikingly, the transcription factors Snail and ZEB-1 share a common target motif—the E-box sequence “CANNTG”. Further, the “GATA” sequence is a motif regulated during EMT across species. While both these target sequences display low complexity, and thus sequence analysis alone is insufficient to predict potential new targets, these motifs should be considered in more detail by studies investigating the transcriptional control of cell polarity determinants.
The E-box binding transcription factor SNAIL is known to target a variety of cell polarity determinants during EMT. The genes encoding DLG1, LLGL2, and CRB3 contain confirmed E-boxes and are directly bound and repressed by SNAIL [50,51,52]. Snail further regulates Baz, Patj and Pals1 [49,52]. While it is unclear whether Patj and Pals1 are directly regulated, Baz is unlikely to be targeted directly. A different study found that the Snail-family transcription factors Snail, Esg, and Wor did not target Baz at the transcriptional level in neuroblasts [66]. Thus, Baz is unlikely to be a direct target of Snail in Drosophila epithelia and neuroblasts.
Together with Esg and Wor, Snail has an important function in the regulation of cell polarity during asymmetric neuroblast division. This triad of transcription factors indirectly activates insc transcription and translation [66,67]. Further, Snail, Esg, and Wor appear to target key factors of the telophase rescue program [66]. Dlg1, one of the components inducing telophase rescue in flies [68,69], is directly repressed by Snail during EMT in a number of different tumors in mammals [50]. In MDCK cells, DLG is mildly downregulated by SNAIL, although it is unclear whether this is an effect of direct SNAIL binding [52]. However, the requirement for Snail, Esg, and Wor for telophase rescue in Drosophila neuroblasts rather points to a positive effect on cell fate determinants such as Dlg1. Hence, the transcriptional repression of dlg1 by Snail is not conserved in neuroblasts and may not be of major relevance in mammalian epithelia either.
In contrast, the crb promoter binding by Snail is conserved between mammals and flies [48,52]. Interestingly, while this binding leads to repression of transcription in MDCK cells, it does not constitutively result in CRB3 repression in breast cancer cells [52,55].
Together, Snail indisputably targets cell polarity determinants in order to regulate EMT. However, which cell polarity determinant is targeted depends on the cell type and organism. While some Snail targets may simply not be conserved, the regulation of crb depicts an example that suggests that Snail targets are context-dependent. It will be interesting to learn about which cofactors regulate the exact choice of Snail target genes.
It is unclear to what degree other mechanisms regulating the expression of polarity determinants are conserved. For example, the FoxA family transcription factor PHA-4 induces polarity determinant expression in the worm [72]. While C. elegans PHA-4 is a driver of organogenesis, the Drosophila PHA-4 homolog Forkhead rather regulates cellular function than cell fate specification [95]. The role of the Forkhead family transcription factors in the induction of polarity determinant expression may therefore not be conserved.
The regulation of the genes encoding the PP2A subunit A is influenced by several transcription factors but the functional implications of these regulatory mechanisms are unclear [92,93]. However, PP2A has been assigned a variety of functions independent of its function in cell polarity [91,94]. Whether one of the transcription factors regulating PP2A-A expression influences cell polarity is unclear. Yet, among the PP2A-A regulating transcription factors, ETS-1 may represent a potentially interesting candidate as it was shown to regulate endothelial cell-matrix adhesion [96].
An intriguing commonality between the examples described above is the fact that in most cases a single or only a few cell polarity determinants are transcriptionally regulated. This is the case during EMT where DLG1 and CRB3 are frequent targets and during asymmetric division where aPKC and insc are common targets. It remains to be investigated why some transcriptional control contributes to the regulation of some polarity determinants more than others. A potential explanation could be that misexpression of transcriptionally regulated polarity determinants is highly unfavorable. For example, dlg1 mutation facilitates tumor development and aPKC and Insc are crucial self-renewal factors during asymmetric stem cell division [20,97]. Otherwise, it might be more energy and/or time efficient for cells to regulate one or only a few key regulators of polarity. Through interactions at the protein level, these key regulators could then organize changes in the polarity system. In addition, in many cases we may simply not yet know of the transcriptional regulation of polarity regulators.

6. Conclusions and Remarks

Many processes require the fine tuning of cell polarity. How polarity regulators are regulated on the protein level is extensively studied and has revealed that many mechanisms regulate polarity regulator activity, stability, localization, and translation. Beyond this, through our literature review we further conclude that transcriptional regulation is decisive for many processes where cell polarity needs to be established, maintained, or abolished. While some transcriptional regulation mechanisms have been described, much remains to be learned about transcriptional regulation of polarity determinants. A particularly interesting task constitutes the investigation of why some polarity determinants are transcriptionally regulated while others are not and how target choices of transcription factors are made based on the cellular context.

Author Contributions

K.R. wrote the original draft of the manuscript and designed the figures. A.W. edited the text and the references. All authors have read and agreed to the published version of the manuscript.

Funding

K.R. is supported by the Promoting Scientific Independence (PSI) Program of the Philipps-University Marburg, Germany. Work in the laboratory of A.W. is supported by the Deutsche Forschungsgemeinschaft (DFG; SPP 1782), by the CECAD cluster of Excellence at the University of Cologne and by the Center for Molecular Medicine (CMMC) at the University of Cologne.

Acknowledgments

We thank Vanessa Holtwick for critical reading and the Rust and Wodarz lab members for discussion.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Campanale, J.P.; Sun, T.Y.; Montell, D.J. Development and dynamics of cell polarity at a glance. J. Cell Sci. 2017, 130, 1201–1207. [Google Scholar] [CrossRef] [Green Version]
  2. Wen, Q.; Mruk, D.; Tang, E.I.; Wong, C.K.C.; Lui, W.-Y.; Lee, W.M.; Xiao, X.; Silvestrini, B.; Cheng, C.Y. Cell polarity and cytoskeletons—Lesson from the testis. Semin. Cell Dev. Biol. 2018, 81, 21–32. [Google Scholar] [CrossRef]
  3. Daynac, M.; Petritsch, C.K. Regulation of Asymmetric Cell Division in Mammalian Neural Stem and Cancer Precursor Cells. Results Probl. Cell Differ. 2017, 61, 375–399. [Google Scholar]
  4. Jossin, Y. Molecular mechanisms of cell polarity in a range of model systems and in migrating neurons. Mol. Cell. Neurosci. 2020, 106, 103503. [Google Scholar] [CrossRef]
  5. Pacquelet, A. Asymmetric Cell Division in the One-Cell C. elegans Embryo: Multiple Steps to Generate Cell Size Asymmetry. Results Probl. Cell Differ. 2017, 61, 115–140. [Google Scholar]
  6. Raman, R.; Pinto, C.S.; Sonawane, M. Polarized Organization of the Cytoskeleton: Regulation by Cell Polarity Proteins. J. Mol. Biol. 2018, 430, 3565–3584. [Google Scholar] [CrossRef] [PubMed]
  7. Krahn, M.P. Phospholipids of the Plasma Membrane—Regulators or Consequence of Cell Polarity? Front. Cell Dev. Biol. 2020, 8, 277. [Google Scholar] [CrossRef]
  8. Burakov, A.V.; Nadezhdina, E.S. Centering and Shifting of Centrosomes in Cells. Cells 2020, 9, 1351. [Google Scholar] [CrossRef] [PubMed]
  9. van IJzendoorn, S.C.D.; Agnetti, J.; Gassama-Diagne, A. Mechanisms behind the polarized distribution of lipids in epithelial cells. Biochim. Biophys. Acta Biomembr. 2020, 1862, 183145. [Google Scholar] [CrossRef] [PubMed]
  10. Hong, Y. aPKC: The Kinase that Phosphorylates Cell Polarity. F1000Research 2018, 7, 903. [Google Scholar] [CrossRef]
  11. Schuhmacher, D.; Sontag, J.-M.; Sontag, E. Protein Phosphatase 2A: More than a Passenger in the Regulation of Epithelial Cell–Cell Junctions. Front. Cell Dev. Biol. 2019, 7, 30. [Google Scholar] [CrossRef] [Green Version]
  12. Wu, Y.; Griffin, E.E. Regulation of Cell Polarity by PAR-1/MARK Kinase. Curr. Top. Dev. Biol. 2017, 123, 365–397. [Google Scholar] [PubMed] [Green Version]
  13. Bórquez, D.A.; González-Billault, C. Regulation of cell polarity by controlled proteolytic systems. Biol. Res. 2011, 44, 35–41. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Moreno-Bueno, G.; Portillo, F.; Cano, A. Transcriptional regulation of cell polarity in EMT and cancer. Oncogene 2008, 27, 6958–6969. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Ahmed, S.M.; Macara, I.G. Mechanisms of polarity protein expression control. Curr. Opin. Cell Biol. 2016, 42, 38–45. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Osswald, M.; Morais-de-Sá, E. Dealing with apical–basal polarity and intercellular junctions: A multidimensional challenge for epithelial cell division. Curr. Opin. Cell Biol. 2019, 60, 75–83. [Google Scholar] [CrossRef]
  17. Persa, O.-D.; Niessen, C.M. Epithelial polarity limits EMT. Nat. Cell Biol. 2019, 21, 299–300. [Google Scholar] [CrossRef]
  18. Fomicheva, M.; Tross, E.M.; Macara, I.G. Polarity proteins in oncogenesis. Curr. Opin. Cell Biol. 2020, 62, 26–30. [Google Scholar] [CrossRef]
  19. Wodarz, A.; Näthke, I. Cell polarity in development and cancer. Nat. Cell Biol. 2007, 9, 1016–1024. [Google Scholar] [CrossRef]
  20. Homem, C.C.F.; Knoblich, J.A. Drosophila neuroblasts: A model for stem cell biology. Development 2012, 139, 4297–4310. [Google Scholar] [CrossRef] [Green Version]
  21. Berika, M.; Elgayyar, M.E.; El-Hashash, A.H.K. Asymmetric cell division of stem cells in the lung and other systems. Front. Cell Dev. Biol. 2014, 2, 33. [Google Scholar] [CrossRef] [Green Version]
  22. Moreno, M.R.; Stempor, P.A.; Bulgakova, N.A. Interactions and Feedbacks in E-Cadherin Transcriptional Regulation. Front. Cell Dev. Biol. 2021, 9, 701175. [Google Scholar] [CrossRef] [PubMed]
  23. Choi, J.; Troyanovsky, R.B.; Indra, I.; Mitchell, B.J.; Troyanovsky, S.M. Scribble, Erbin, and Lano redundantly regulate epithelial polarity and apical adhesion complex. J. Cell Biol. 2019, 218, 2277–2293. [Google Scholar] [CrossRef] [Green Version]
  24. Tepass, U. The apical polarity protein network in Drosophila epithelial cells: Regulation of polarity, junctions, morphogenesis, cell growth, and survival. Annu. Rev. Cell Dev. Biol. 2012, 28, 655–685. [Google Scholar] [CrossRef] [PubMed]
  25. Riga, A.; Castiglioni, V.G.; Boxem, M. New insights into apical-basal polarization in epithelia. Curr. Opin. Cell Biol. 2020, 62, 1–8. [Google Scholar] [CrossRef]
  26. Wen, W.; Zhang, M. Protein Complex Assemblies in Epithelial Cell Polarity and Asymmetric Cell Division. J. Mol. Biol. 2018, 430, 3504–3520. [Google Scholar] [CrossRef]
  27. Plant, P.J.; Fawcett, J.P.; Lin, D.C.C.; Holdorf, A.D.; Binns, K.; Kulkarni, S.; Pawson, T. A polarity complex of mPar-6 and atypical PKC binds, phosphorylates and regulates mammalian Lgl. Nat. Cell Biol. 2003, 5, 301–308. [Google Scholar] [CrossRef]
  28. Betschinger, J.; Mechtler, K.; Knoblich, J.A. The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl. Nature 2003, 422, 326–330. [Google Scholar] [CrossRef] [PubMed]
  29. Wirtz-Peitz, F.; Nishimura, T.; Knoblich, J.A. Linking Cell Cycle to Asymmetric Division: Aurora-A Phosphorylates the Par Complex to Regulate Numb Localization. Cell 2008, 135, 161–173. [Google Scholar] [CrossRef] [Green Version]
  30. Bulgakova, N.A.; Knust, E. The Crumbs complex: From epithelial-cell polarity to retinal degeneration. J. Cell Sci. 2009, 122, 2587–2596. [Google Scholar] [CrossRef] [Green Version]
  31. de Almeida, F.N.; Walther, R.F.; Pressé, M.T.; Vlassaks, E.; Pichaud, F. Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs. Development 2019, 146, dev175497. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  32. Golub, O.; Wee, B.; Newman, R.A.; Paterson, N.M.; Prehoda, K.E. Activation of Discs large by aPKC aligns the mitotic spindle to the polarity axis during asymmetric cell division. eLife 2017, 6, e32137. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Krahn, M.P.; Egger-Adam, D.; Wodarz, A. PP2A antagonizes phosphorylation of Bazooka by PAR-1 to control apical-basal polarity in dividing embryonic neuroblasts. Dev. Cell 2009, 16, 901–908. [Google Scholar] [CrossRef] [Green Version]
  34. Ogawa, H.; Ohta, N.; Moon, W.; Matsuzaki, F. Protein phosphatase 2A negatively regulates aPKC signaling by modulating phosphorylation of Par-6 in Drosophila neuroblast asymmetric divisions. J. Cell Sci. 2009, 122, 3242–3249. [Google Scholar] [CrossRef] [Green Version]
  35. Nunbhakdi-Craig, V.; Machleidt, T.; Ogris, E.; Bellotto, D.; White, C.L., 3rd; Sontag, E. Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex. J. Cell Biol. 2002, 15, 967–978. [Google Scholar] [CrossRef] [Green Version]
  36. Thompson, B.J. Par-3 family proteins in cell polarity & adhesion. FEBS J. 2021. [Google Scholar] [CrossRef]
  37. Drummond, M.L.; Prehoda, K.E. Molecular Control of Atypical Protein Kinase C: Tipping the Balance between Self-Renewal and Differentiation. J. Mol. Biol. 2016, 428, 1455–1464. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Bonello, T.T.; Peifer, M. Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation. J. Cell Biol. 2019, 218, 742–756. [Google Scholar] [CrossRef] [PubMed]
  39. Huang, R.Y.-J.; Guilford, P.; Thiery, J.P. Early events in cell adhesion and polarity during epithelial-mesenchymal transition. J. Cell Sci. 2012, 125, 4417–4422. [Google Scholar] [CrossRef] [Green Version]
  40. Diepenbruck, M.; Christofori, G. Epithelial-mesenchymal transition (EMT) and metastasis: Yes, no, maybe? Curr. Opin. Cell Biol. 2016, 43, 7–13. [Google Scholar] [CrossRef] [Green Version]
  41. Russell, H.; Pranjol, M.Z.I. Transcription factors controlling E-cadherin down-regulation in ovarian cancer. Biosci. Horiz. 2018, 11, hzy010. [Google Scholar] [CrossRef] [Green Version]
  42. Wong, T.-S.; Gao, W.; Chan, J.Y.-W. Transcription regulation of E-cadherin by zinc finger E-box binding homeobox proteins in solid tumors. BioMed Res. Int. 2014, 2014, 921564. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Peinado, H.; Portillo, F.; Cano, A. Transcriptional regulation of cadherins during development and carcinogenesis. Int. J. Dev. Biol. 2004, 48, 365–375. [Google Scholar] [CrossRef] [Green Version]
  44. Kuphal, S.; Bosserhoff, A.K. Influence of the cytoplasmic domain of E-cadherin on endogenous N-cadherin expression in malignant melanoma. Oncogene 2006, 25, 248–259. [Google Scholar] [CrossRef] [PubMed]
  45. Pires, B.R.B.; Mencalha, A.L.; Ferreira, G.M.; de Souza, W.F.; Morgado-Díaz, J.A.; Maia, A.M.; Corrêa, S.; Abdelhay, E.S.F.W. NF-kappaB Is Involved in the Regulation of EMT Genes in Breast Cancer Cells. PLoS ONE 2017, 12, e0169622. [Google Scholar] [CrossRef] [Green Version]
  46. Kuphal, S.; Poser, I.; Jobin, C.; Hellerbrand, C.; Bosserhoff, A.K. Loss of E-cadherin leads to upregulation of NFκB activity in malignant melanoma. Oncogene 2004, 23, 8509–8519. [Google Scholar] [CrossRef] [Green Version]
  47. Wu, Y.; Zhou, B.P. Snail: More than EMT. Cell Adh. Migr. 2010, 4, 199–203. [Google Scholar] [CrossRef]
  48. Campbell, K.; Lebreton, G.; Franch-Marro, X.; Casanova, J. Differential roles of the Drosophila EMT-inducing transcription factors Snail and Serpent in driving primary tumour growth. PLoS Genet. 2018, 14, e1007167. [Google Scholar] [CrossRef] [Green Version]
  49. Weng, M.; Wieschaus, E. Polarity protein Par3/Bazooka follows myosin-dependent junction repositioning. Dev. Biol. 2017, 422, 125–134. [Google Scholar] [CrossRef] [PubMed]
  50. Cavatorta, A.L.; Giri, A.A.; Banks, L.; Gardiol, D. Cloning and functional analysis of the promoter region of the human Disc large gene. Gene 2008, 424, 87–95. [Google Scholar] [CrossRef]
  51. Kashyap, A.; Zimmerman, T.; Ergül, N.; Bosserhoff, A.; Hartman, U.; Alla, V.; Bataille, F.; Galle, P.R.; Strand, S.; Strand, D. The human Lgl polarity gene, Hugl-2, induces MET and suppresses Snail tumorigenesis. Oncogene 2013, 32, 1396–1407. [Google Scholar] [CrossRef] [Green Version]
  52. Whiteman, E.L.; Liu, C.-J.; Fearon, E.R.; Margolis, B. The transcription factor snail represses Crumbs3 expression and disrupts apico-basal polarity complexes. Oncogene 2008, 27, 3875–3879. [Google Scholar] [CrossRef] [Green Version]
  53. Arefin, B.; Parvin, F.; Bahrampour, S.; Stadler, C.B.; Thor, S. Drosophila Neuroblast Selection Is Gated by Notch, Snail, SoxB, and EMT Gene Interplay. Cell Rep. 2019, 29, 3636–3651.e3. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Aigner, K.; Dampier, B.; Descovich, L.; Mikula, M.; Sultan, A.; Schreiber, M.; Mikulits, W.; Brabletz, T.; Strand, D.; Obrist, P.; et al. The transcription factor ZEB1 (deltaEF1) promotes tumour cell dedifferentiation by repressing master regulators of epithelial polarity. Oncogene 2007, 26, 6979–6988. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Alam, M.; Bouillez, A.; Tagde, A.; Ahmad, R.; Rajabi, H.; Maeda, T.; Hiraki, M.; Suzuki, Y.; Kufe, D. MUC1-C Represses the Crumbs Complex Polarity Factor CRB3 and Downregulates the Hippo Pathway. Mol. Cancer Res. 2016, 14, 1266–1276. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  56. Li, P.; Zhou, C.; Yan, Y.; Li, J.; Liu, J.; Zhang, Y.; Liu, P. Crumbs protein homolog 3 (CRB3) expression is associated with oestrogen and progesterone receptor positivity in breast cancer. Clin. Exp. Pharmacol. Physiol. 2019, 46, 837–844. [Google Scholar] [CrossRef]
  57. Campbell, K.; Whissell, G.; Franch-Marro, X.; Batlle, E.; Casanova, J. Specific GATA Factors Act as Conserved Inducers of an Endodermal-EMT. Dev. Cell 2011, 21, 1051–1061. [Google Scholar] [CrossRef] [PubMed]
  58. Chang, K.C.; Garcia-Alvarez, G.; Somers, G.; Sousa-Nunes, R.; Rossi, F.; Lee, Y.Y.; Soon, S.B.; Gonzalez, C.; Chia, W.; Wang, H. Interplay between the transcription factor Zif and aPKC regulates neuroblast polarity and self-renewal. Dev. Cell 2010, 19, 778–785. [Google Scholar] [CrossRef] [PubMed]
  59. Rust, K.; Tiwari, M.D.; Mishra, V.K.; Grawe, F.; Wodarz, A. Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila. EMBO J. 2018, 37, e98659. [Google Scholar] [CrossRef] [PubMed]
  60. Fazzio, T.G.; Huff, J.T.; Panning, B. An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity. Cell 2008, 134, 162–174. [Google Scholar] [CrossRef] [Green Version]
  61. Chen, P.B.; Hung, J.-H.; Hickman, T.L.; Coles, A.H.; Carey, J.F.; Weng, Z.; Chu, F.; Fazzio, T.G. Hdac6 regulates Tip60-p400 function in stem cells. eLife 2013, 2, e01557. [Google Scholar] [CrossRef]
  62. Mah, I.K.; Soloff, R.; Hedrick, S.M.; Mariani, F.V. Atypical PKC-iota Controls Stem Cell Expansion via Regulation of the Notch Pathway. Stem Cell Rep. 2015, 5, 866–880. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  63. Boroviak, T.; Rashbass, P. The Apical Polarity Determinant Crumbs 2 Is a Novel Regulator of ESC-Derived Neural Progenitors. Stem Cells 2011, 29, 193–205. [Google Scholar] [CrossRef]
  64. Izumi, H.; Kaneko, Y.; Nakagawara, A. The Role of MYCN in Symmetric vs. Asymmetric Cell Division of Human Neuroblastoma Cells. Front. Oncol. 2020, 10, 570815. [Google Scholar] [CrossRef] [PubMed]
  65. Ishibashi, R.; Kozuki, S.; Kamakura, S.; Sumimoto, H.; Toyoshima, F. c-Rel Regulates Inscuteable Gene Expression during Mouse Embryonic Stem Cell Differentiation. J. Biol. Chem. 2016, 291, 3333–3345. [Google Scholar] [CrossRef] [Green Version]
  66. Cai, Y.; Chia, W.; Yang, X. A family of snail-related zinc finger proteins regulates two distinct and parallel mechanisms that mediate Drosophila neuroblast asymmetric divisions. EMBO J. 2001, 20, 1704–1714. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  67. Ashraf, S.I.; Ip, Y.T. The Snail protein family regulates neuroblast expression of inscuteable and string, genes involved in asymmetry and cell division in Drosophila. Development 2001, 128, 4757–4767. [Google Scholar] [CrossRef]
  68. Peng, C.Y.; Manning, L.; Albertson, R.; Doe, C.Q. The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts. Nature 2000, 408, 596–600. [Google Scholar] [CrossRef] [PubMed]
  69. Siegrist, S.E.; Doe, C.Q. Microtubule-induced pins/Galphai cortical polarity in Drosophila neuroblasts. Cell 2005, 123, 1323–1335. [Google Scholar] [CrossRef] [Green Version]
  70. Marziali, F.; Cavatorta, A.L.; Valdano, M.B.; Facciuto, F.; Gardiol, D. Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes. Biol. Chem. 2015, 396, 893–902. [Google Scholar] [CrossRef]
  71. Cavatorta, A.L.; Facciuto, F.; Valdano, M.B.; Marziali, F.; Giri, A.A.; Banks, L.; Gardiol, D. Regulation of translational efficiency by different splice variants of the Disc large 1 oncosuppressor 5′-UTR. FEBS J. 2011, 278, 2596–2608. [Google Scholar] [CrossRef]
  72. Von Stetina, S.E.; Liang, J.; Marnellos, G.; Mango, S.E. Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6. Mol. Biol. Cell 2017, 28, 2042–2065. [Google Scholar] [CrossRef]
  73. Watts, J.L.; Etemad-Moghadam, B.; Guo, S.; Boyd, L.; Draper, B.W.; Mello, C.C.; Priess, J.R.; Kemphues, K.J. par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3. Development 1996, 122, 3133–3140. [Google Scholar] [CrossRef]
  74. Nance, J.; Munro, E.M.; Priess, J.R. C. elegans PAR-3 and PAR-6 are required for apicobasal asymmetries associated with cell adhesion and gastrulation. Development 2003, 130, 5339–5350. [Google Scholar] [CrossRef] [Green Version]
  75. Totong, R.; Achilleos, A.; Nance, J. PAR-6 is required for junction formation but not apicobasal polarization in C. elegans embryonic epithelial cells. Development 2007, 134, 1259–1268. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  76. Portereiko, M.F.; Saam, J.; Mango, S.E. ZEN-4/MKLP1 is required to polarize the foregut epithelium. Curr. Biol. 2004, 14, 932–941. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  77. Organista, M.F.; Martín, M.; de Celis, J.M.; Barrio, R.; López-Varea, A.; Esteban, N.; Casado, M.; de Celis, J.F. The Spalt Transcription Factors Generate the Transcriptional Landscape of the Drosophila melanogaster Wing Pouch Central Region. PLoS Genet. 2015, 11, e1005370. [Google Scholar] [CrossRef] [Green Version]
  78. McDermott, S.M.; Yang, L.; Halstead, J.M.; Hamilton, R.S.; Meignin, C.; Davis, I. Drosophila Syncrip modulates the expression of mRNAs encoding key synaptic proteins required for morphology at the neuromuscular junction. RNA 2014, 20, 1593–1606. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  79. Hengst, U.; Deglincerti, A.; Kim, H.J.; Jeon, N.L.; Jaffrey, S.R. Axonal elongation triggered by stimulus-induced local translation of a polarity complex protein. Nat. Cell Biol. 2009, 11, 1024–1030. [Google Scholar] [CrossRef] [PubMed]
  80. Horne-Badovinac, S.; Bilder, D. Dynein regulates epithelial polarity and the apical localization of stardust A mRNA. PLoS Genet. 2008, 4, e8. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  81. Li, Z.; Wang, L.; Hays, T.S.; Cai, Y. Dynein-mediated apical localization of crumbs transcripts is required for Crumbs activity in epithelial polarity. J. Cell Biol. 2008, 180, 31–38. [Google Scholar] [CrossRef] [Green Version]
  82. Jiang, K.; Liu, Y.; Fan, J.; Epperly, G.; Gao, T.; Jiang, J.; Jia, J. Hedgehog-regulated atypical PKC promotes phosphorylation and activation of Smoothened and Cubitus interruptus in Drosophila. Proc. Natl. Acad. Sci. USA 2014, 111, E4842–E4850. [Google Scholar] [CrossRef] [Green Version]
  83. Atwood, S.X.; Li, M.; Lee, A.; Tang, J.Y.; Oro, A.E. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013, 494, 484–488. [Google Scholar] [CrossRef] [Green Version]
  84. Fournier-Thibault, C.; Blavet, C.; Jarov, A.; Bajanca, F.; Thorsteinsdóttir, S.; Duband, J.-L. Sonic hedgehog regulates integrin activity, cadherin contacts, and cell polarity to orchestrate neural tube morphogenesis. J. Neurosci. 2009, 29, 12506–12520. [Google Scholar] [CrossRef] [Green Version]
  85. Djiane, A.; Yogev, S.; Mlodzik, M. The apical determinants aPKC and dPatj regulate Frizzled-dependent planar cell polarity in the Drosophila eye. Cell 2005, 121, 621–631. [Google Scholar] [CrossRef] [Green Version]
  86. Qian, L.; Wythe, J.D.; Liu, J.; Cartry, J.; Vogler, G.; Mohapatra, B.; Otway, R.T.; Huang, Y.; King, I.N.; Maillet, M.; et al. Tinman/Nkx2-5 acts via miR-1 and upstream of Cdc42 to regulate heart function across species. J. Cell Biol. 2011, 193, 1181–1196. [Google Scholar] [CrossRef] [Green Version]
  87. Li, J.; Liu, Y.; Jin, Y.; Wang, R.; Wang, J.; Lu, S.; VanBuren, V.; Dostal, D.E.; Zhang, S.L.; Peng, X. Essential role of Cdc42 in cardiomyocyte proliferation and cell-cell adhesion during heart development. Dev. Biol. 2017, 421, 271–283. [Google Scholar] [CrossRef]
  88. Lovegrove, B.; Simões, S.; Rivas, M.L.; Sotillos, S.; Johnson, K.; Knust, E.; Jacinto, A.; Hombría, J.C.-G. Coordinated control of cell adhesion, polarity, and cytoskeleton underlies Hox-induced organogenesis in Drosophila. Curr. Biol. 2006, 16, 2206–2216. [Google Scholar] [CrossRef]
  89. Hombría, J.C.G.; Rivas, M.L.; Sotillos, S. Genetic control of morphogenesis—Hox induced organogenesis of the posterior spiracles. Int. J. Dev. Biol. 2009, 53, 1349–1358. [Google Scholar] [CrossRef] [Green Version]
  90. Traweger, A.; Wiggin, G.; Taylor, L.; Tate, S.A.; Metalnikov, P.; Pawson, T. Protein phosphatase 1 regulates the phosphorylation state of the polarity scaffold Par-3. Proc. Natl. Acad. Sci. USA 2008, 105, 10402–10407. [Google Scholar] [CrossRef] [Green Version]
  91. Thompson, J.J.; Williams, C.S. Protein Phosphatase 2A in the Regulation of Wnt Signaling, Stem Cells, and Cancer. Genes 2018, 9, 121. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  92. Chen, H.-G.; Han, W.-J.; Deng, M.; Qin, J.; Yuan, D.; Liu, J.-P.; Xiao, L.; Gong, L.; Liang, S.; Zhang, J.; et al. Transcriptional regulation of PP2A-A alpha is mediated by multiple factors including AP-2alpha, CREB, ETS-1, and SP-1. PLoS ONE 2009, 4, e7019. [Google Scholar] [CrossRef] [PubMed]
  93. Liu, J.; Ji, W.; Sun, S.; Zhang, L.; Chen, H.-G.; Mao, Y.; Liu, L.; Zhang, X.; Gong, L.; Deng, M.; et al. The PP2A-Aβ gene is regulated by multiple transcriptional factors including Ets-1, SP1/SP3, and RXRα/β. Curr. Mol. Med. 2012, 12, 982–994. [Google Scholar] [CrossRef] [PubMed]
  94. Wlodarchak, N.; Xing, Y. PP2A as a master regulator of the cell cycle. Crit. Rev. Biochem. Mol. Biol. 2016, 51, 162–184. [Google Scholar] [CrossRef]
  95. Maruyama, R.; Grevengoed, E.; Stempniewicz, P.; Andrew, D.J. Genome-wide analysis reveals a major role in cell fate maintenance and an unexpected role in endoreduplication for the Drosophila FoxA gene Fork head. PLoS ONE 2011, 6, e20901. [Google Scholar] [CrossRef] [Green Version]
  96. Mattot, V.; Vercamer, C.; Soncin, F.; Calmels, T.; Huguet, C.; Fafeur, V.; Vandenbunder, B. Constitutive expression of the DNA-binding domain of Ets1 increases endothelial cell adhesion and stimulates their organization into capillary-like structures. Oncogene 2000, 19, 762–772. [Google Scholar] [CrossRef] [Green Version]
  97. Humbert, P.O.; Grzeschik, N.A.; Brumby, A.M.; Galea, R.; Elsum, I.; Richardson, H.E. Control of tumourigenesis by the Scribble/Dlg/Lgl polarity module. Oncogene 2008, 27, 6888–6907. [Google Scholar] [CrossRef] [Green Version]
Figure 1. Overview of cell polarity determinants in Drosophila epithelia and neuroblasts. (A) Epithelial cells are polarized along the apicobasal axis. The Crb and Par complexes localize apically, and components of these complexes can interact with each other. In Drosophila, this region is referred to as the subapical region (SAR). In addition, Baz/Par-3 interacts with adherens junctions (AJ). The Par complex kinase aPKC phosphorylates Lgl, restricting the Scrib complex to the basolateral side of the cell, where it localizes cortically with the other members of the Scrib complex: Scrib and Dlg. At the basolateral side of the cell Lgl inhibits aPKC. (B) The Drosophila neuroblast (NB) is widely used as a model to study asymmetric stem cell division. Mitotic neuroblasts display apicobasal polarity. Components of the Par and Scrib complexes are apically localized. Further, Insc and its interactor Pins localize apically and orient the spindle apparatus. Neuroblast division results in two distinct daughter cells: the apical cell inherits apical determinants and maintains neuroblast fate. The basally formed cell, called ganglion mother cell (GMC), inherits basally localized differentiation factors and divides to produce neurons.
Figure 1. Overview of cell polarity determinants in Drosophila epithelia and neuroblasts. (A) Epithelial cells are polarized along the apicobasal axis. The Crb and Par complexes localize apically, and components of these complexes can interact with each other. In Drosophila, this region is referred to as the subapical region (SAR). In addition, Baz/Par-3 interacts with adherens junctions (AJ). The Par complex kinase aPKC phosphorylates Lgl, restricting the Scrib complex to the basolateral side of the cell, where it localizes cortically with the other members of the Scrib complex: Scrib and Dlg. At the basolateral side of the cell Lgl inhibits aPKC. (B) The Drosophila neuroblast (NB) is widely used as a model to study asymmetric stem cell division. Mitotic neuroblasts display apicobasal polarity. Components of the Par and Scrib complexes are apically localized. Further, Insc and its interactor Pins localize apically and orient the spindle apparatus. Neuroblast division results in two distinct daughter cells: the apical cell inherits apical determinants and maintains neuroblast fate. The basally formed cell, called ganglion mother cell (GMC), inherits basally localized differentiation factors and divides to produce neurons.
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Table 1. Apicobasal polarity proteins are conserved across species.
Table 1. Apicobasal polarity proteins are conserved across species.
HumanD. melanogasterC. elegansUpstream Transcription Factors
Par complex
PARD3bazpar-3Snail (Drosophila, gastrulation)
PHA-4 (C. elegans, epithelium)
PARD6Apar-6par-6
PKCλ, PKCζaPKCpkc-3Zif (Drosophila, neuroblast)
Myc-Tip60 (Drosophila, neuroblast)
ci (Drosophila, wing)
CDC42cdc42cdc-42Tinman/Nkx2-5 (Drosophila/mouse, heart development)
Scrib complex
SCRIB *scriblet-413Salm and Salr (Drosophila, wing)
DLG1, DLG2, DLG3, DLG4dlg1dlg-1Snail (human, tumorigenesis)
PHA-4 (C. elegans, epithelium)
Salm and Salr (Drosophila, wing)
LLGL1, LLGL2l(2)gllgl-1Snail (human, breast cancer)
ZEB-1 (human, breast cancer)
Crumbs complex
CRB1, CRB2, CRB3crbcrb-1, crb-3Snail (MDCK)
ZEB-1 (human, breast cancer)
MUC1-C (human, breast cancer)
ERα (human, breast cancer)
hGATA6/Srp (human/Drosophila, EMT)
Stat92E (Drosophila, spiracle)
PALS1sdtmagu-2Snail (MDCK)
Srp (Drosophila, EMT)
PATJpatjmpz-1Snail (MDCK)
ZEB-1 (human, breast cancer)
LIN7A, LIN7B, LIN7Cvelilin-7
Selected interactors of polarity complexes
INSCinscinsc-1c-Rel (mouse, ESC)
Escargot and Snail and Worniu
(Drosophila, neuroblast)
CDH1shghmr-1SNAIL, SLUG, ZEB-1, ZEB-2, Twist1/2, RUNX1, FOXA, p300, Rb, c-Myc, AP-2 (recently reviewed in [22])
ct, Stat92E (Drosophila, spiracle)
CDH2CadNhmr-1NFκB
PPP2R2/3/5/6
PPP2CA/B
PPP2R1A/B
Pp2A-29B, mts, wrdlet-92, paa-1, sur-6, pptr-1/2, rsa-1, cash-1CREB, ETS-1, AP-2α,
SP-1, SP-3, RXRα/β
(mammal, epithelium)
The table lists genes encoding cell polarity determinants and their orthologs in human, Drosophila melanogaster, and Caenorhabditis elegans. Upstream regulators are bold when they are known to directly affect transcription of their targets, underlined when they affect their target indirectly and in normal letters when the exact mechanism is unclear. Magenta text indicates repression, green text indicates activation of gene expression. * Two related proteins, ERBIN (enoded by ERBIN) and LANO (encoded by LRRC1), act redundantly with SCRIB in mammals [23]. MDCK: Madin-Darby Canine Kidney cells.
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Rust, K.; Wodarz, A. Transcriptional Control of Apical-Basal Polarity Regulators. Int. J. Mol. Sci. 2021, 22, 12340. https://doi.org/10.3390/ijms222212340

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Rust K, Wodarz A. Transcriptional Control of Apical-Basal Polarity Regulators. International Journal of Molecular Sciences. 2021; 22(22):12340. https://doi.org/10.3390/ijms222212340

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Rust, Katja, and Andreas Wodarz. 2021. "Transcriptional Control of Apical-Basal Polarity Regulators" International Journal of Molecular Sciences 22, no. 22: 12340. https://doi.org/10.3390/ijms222212340

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