Enterococcus faecium Regulates Honey Bee Developmental Genes
Abstract
:1. Introduction
2. Results
2.1. Draft Genome Sequence of E. faecium Isolated from Honey Bee Gut
2.2. E. faecium Increased Gut Weight of Honey Bee and Activated Host Developmental Gene Expression
2.3. MicroRNAs Participated in Host Developmental Pathway Regulation during E. faecium Colonization
3. Discussion
4. Materials and Methods
4.1. Bacterial Strain Isolation and Culture
4.2. Bacterial DNA Extraction and Genome Sequencing
4.3. Bacterial Genome Assembly
4.4. Structural and Functional Annotations for Bacterial Genomes
4.5. Phylogenetic Analysis
4.6. Generation of Microbiota-Free (MF) and E. faecium-Colonized (En) Bees
4.7. Extraction of DNA and RNA from Honey Bee Hindgut
4.8. Quantitative PCR (qPCR) for Determination of Gut Bacterial Loads
4.9. 16S Amplicon Sequence Analysis
4.10. RNA-Seq Analysis
4.11. Small RNA-Seq Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Du, Y.; Luo, S.; Zhou, X. Enterococcus faecium Regulates Honey Bee Developmental Genes. Int. J. Mol. Sci. 2021, 22, 12105. https://doi.org/10.3390/ijms222212105
Du Y, Luo S, Zhou X. Enterococcus faecium Regulates Honey Bee Developmental Genes. International Journal of Molecular Sciences. 2021; 22(22):12105. https://doi.org/10.3390/ijms222212105
Chicago/Turabian StyleDu, Yating, Shiqi Luo, and Xin Zhou. 2021. "Enterococcus faecium Regulates Honey Bee Developmental Genes" International Journal of Molecular Sciences 22, no. 22: 12105. https://doi.org/10.3390/ijms222212105