Next Article in Journal
Hexavalent Chromium Removal from Water and Wastewaters by Electrochemical Processes: Review
Previous Article in Journal
Deep Eutectic Liquids as a Topical Vehicle for Tadalafil: Characterisation and Potential Wound Healing and Antimicrobial Activity
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 28
Issue 5
10.3390/molecules28052409
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Nematode Pheromones: Structures and Functions

by
Biyuan Yang
†,
Jie Wang
†,
Xi Zheng
and
Xin Wang
*
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2023, 28(5), 2409; https://doi.org/10.3390/molecules28052409
Submission received: 29 January 2023 / Revised: 1 March 2023 / Accepted: 4 March 2023 / Published: 6 March 2023
Browse Figures
Review Reports Versions Notes

Abstract

:
Pheromones are chemical signals secreted by one individual that can affect the behaviors of other individuals within the same species. Ascaroside is an evolutionarily conserved family of nematode pheromones that play an integral role in the development, lifespan, propagation, and stress response of nematodes. Their general structure comprises the dideoxysugar ascarylose and fatty-acid-like side chains. Ascarosides can vary structurally and functionally according to the lengths of their side chains and how they are derivatized with different moieties. In this review, we mainly describe the chemical structures of ascarosides and their different effects on the development, mating, and aggregation of nematodes, as well as how they are synthesized and regulated. In addition, we discuss their influences on other species in various aspects. This review provides a reference for the functions and structures of ascarosides and enables their better application.

Graphical Abstract

1. Introduction

Nematodes are the most abundant animals on Earth, and they can be found virtually everywhere on land and in water [1]. Nematodes have a variety of lifestyles; some nematodes live freely, such as Caenorhabditis elegans, whereas some nematodes show parasitic lifestyles, such as root-knot nematodes (RKNs) and cyst nematodes (CNs). The impacts of nematodes extend to various domains of life. Plant parasitic nematodes cause a destructive loss in crop productivity, and, on the contrary, entomopathogenic nematodes can be used to kill insect pests in agriculture [2,3]. There are numerous chemicals that are involved in all aspects of nematode communication and life, mainly pheromones.
Pheromones are chemicals or mixtures of chemicals that can function as communication agents. The use of pheromones is very widespread in nature, for example, in protozoan ciliates, pheromones have functions in self/nonself recognition, vegetative reproduction, and mating interactions [4,5]. The fall armyworm Spodoptera frugiperda can accurately monitor its field population dynamics using sex pheromones [6]. In the locusts Locusta migratoria, only the existence of gregarious male adults can stimulate the synchronization of the sexual maturity of female adults. Among a large number of volatiles released by gregarious male adults, the aggregation pheromone 4-vinyl anisole is considered to play a key role in inducing the synchronization of female sexual maturity [7]. Using this function allows for better control of insect population densities, which also helps to protect crop yields. Pheromones also play a very important role in vertebrates. For example, adult sea lampreys Petromyzon marinus release large quantities of bile acid pheromones that attract mature females [8]. The pheromones (Z)-7-dodecenyl acetate and frontalin have been found in Asian elephants and have specific effects on elephant sexual behavior [9].
Moreover, pheromones also influence nematodes in various ways [10,11]. Unlike other animal species, ascarosides are the main type of pheromone produced by nematodes. These ascarosides comprise fatty-acid-derived side chains attached to the 3,6-dideoxysugar L-ascarylose, and their C-terminus or four loci can be modified [12]. Interestingly, these glycolipids are found almost exclusively in nematodes, including free-living nematodes and nematode species that parasitize insects, vertebrates, and plants [13]. In addition, a few other small molecules function as pheromones in nematodes, but there are relatively few reports on these molecules. Nematode pheromones are capable of regulating many aspects, including their development, mating, aggregation, and many others.
To date, several different nomenclatures have been used for ascarosides. First, one nomenclature system combines the functions of the compounds. For instance, the dauer-inducing ascr#1, ascr#2, and ascr#3 have been referred to as “daumone-1”, “daumone-2”, and “daumone-3”. Second, there is a nomenclature based on chemical structure. For example, according to the length of the carbon side chains on ascarylose sugar, the seven-carbon ascr#1 was named “C7” and the three-carbon ascr#5 was named “C3” [14]. However, as an increasing number of ascaroside structures have been identified, a third nomenclature (small molecule identifiers, SMIDs) has been adopted to name nematode metabolites. The overall structural class of a compound is denoted by four lowercase, non-italic letters in SMIDs, and a pound sign and a number are included, e.g., ascr#1 and icas#9. All assigned SMIDs can be found in the SMID database (www.smid-db.org) [15]. This third nomenclature no longer refers to the function or molecular structure of ascarosides, but to the order of their discovery, for example, ascr#1, 2, 3 … n, and then distinct abbreviations are used for ascarylose-containing molecules that contain additional moieties, such as when the sugar is decorated with an indol (icas#), octopamine succinate (osas#), hydroxybenzoyl (hbas#), or methyl-butenoyl (mbas#) [16].
The pheromones secreted by nematodes play an integral role in their communication and social behaviors. The research on nematode pheromones not only facilitates the use of pheromones for biological control but also serves as a useful reference for understanding the structures and functions of pheromones in the future. The current research has focused on model organisms such as C. elegans, and comparatively little research has been conducted on other species of nematodes. In this paper, we review the structures and functions of different nematode pheromones.

2. Synthesis and Regulation of Ascarosides

Many primary metabolic pathways participate in the synthesis of ascarosides, including the tricarboxylic acid cycle, amino acid catabolism, the peroxisomal β-oxidation of long-chain fatty acids, etc. There are four genes (dhs-28, acox-1, daf-22, and maoc-1) and complex signaling pathways (steroid hormones, serotonin, cGMP, TGF-β, insulin/IGF signaling, etc.) that are involved in ascaroside synthesis in C. elegans [11,14,17,18]. It has been shown that acyl coenzyme A oxidase ACOX-1, enoyl coenzyme A hydratase MAOC-1, β-hydroxyacyl-CoA dehydrogenase DHS-28, and β-ketoacyl -CoA thiolase DAF-22 primarily act in each step of the β-oxidation cycle [19]. The ACOX-1 encoded by the acox-1 gene is the main enzyme for the synthesis of ascarosides in C. elegans [20]. It has fatty acid oxidation activity and interacts with the peroxide PEX-5 in peroxidase bodies [21]. The MAOC-1 is necessary for the biosynthesis of ascarosides’ fatty-acid-derived side chains via peroxisomal β-oxidation, and maoc-1 is the gene related to the regulation of this process [22]. Meanwhile, thiolase DAF-22, a down-regulating factor for the beta-oxidation of the C. elegans peroxidase body, represents a single gene in C. elegans and two genes (Ppa-daf-22.1 and Ppa-daf-22.2) with different domains in the free-living nematode Pristionchus pacificus [23]. Under conditions of adequate nutrition, the biosynthesis of ascarosides is carried out only by Ppa-daf-22.1. In contrast, Ppa-daf-22.2 is induced in the absence of food, leading to the production of specific ascarosides [23]. Dhs-28 encodes a homolog of human D-bifunctional protein that functions upstream of SCPx and is also necessary for pheromone production [24]. Finally, the related reactions regulated by these four genes greatly affect the synthetic process of ascaroside pheromones in C. elegans.
Ascaroside pheromones can perform their biological functions by modulating signaling pathways participating in neuronal transmission. The main pathways for metabolizing ascarosides are as follows: the GPCR-Gqα [25], DAF-7/TGF-β [26], MAPK [27], DAF-2/Insulin pathways, etc. [28]. These pathways also participate in the adjustment of many neurons, mainly including the neurons AWA, ASH, ASI, and ADL and male-specific CEM neurons [11,14,17,18,29,30]. These neurons mainly regulate physiological activities together with related receptor genes. For example, C. elegans hermaphroditism, which acts mainly through self-fertilization, increases the mating rate in males after pathogen exposure, and this increase requires str-44 in AWA neurons [31]. During the induction of ascr#10, C. elegans triggers the ADL sensory neuron process for signal transduction; this process triggers the main mod-1 receptor to respond [32]. When ascarosides act on adults, they attenuate the expression of the insulin peptide INS-6 in ASI chemosensory neurons, resulting in a decrease in neuroendocrine insulin signals, which in turn prolongs reproductive duration [33]. The crh-1 gene of C. elegans autonomously functions in ascr#5-sensing ASI neurons and inhibiting the formation of L2d [26]. In C. elegans, the tyra-2 receptor (a neurotransmitter-sensitive G-protein-coupled receptor) in ASH cells of nociceptive neurons is involved in the induction of osas#9 avoidance expression [34]. Ascarosides reversibly inhibit the expression of the str-3 chemoreceptor gene in ASI neurons. At the same time, the suppression of str-3 requires the involvement of the pheromone receptors SRBC-64/66 and SRG-36/37 [35]. However, more work is needed to clarify the specific mechanisms of these processes in nematodes.

3. Pheromones Secreted by Nematodes

Ascarosides (ASCRs) represent the majority of the pheromones secreted by nematodes. The molecular formula for an ascaroside, C33H68O4, was first proposed by Schulz and Becker in 1933. In 1957, Fairbairn et al. determined the structural formula for ascarosides. Through more in-depth studies on nematode pheromones, it was found that ascaroside derivatives, such as indole ascarosides (ICASs) and the ω-1 oxidation isomers of ASCRs, named OSCRs, can also act as pheromone components. Different phenotypes of nematode species are produced by different ascarosides or combinations of ascarosides; even slight changes in the chemical structure tend to produce drastically different patterns of activity. As a rule, the patterns of the biosynthesis of ascarosides are linked to the phylogeny, lifestyle, and ecological niche of the organism [14,36,37]. In addition, different concentrations of the same ascarosides can have different effects on nematodes. Other chemicals such as vanillic acid function as pheromones in some nematodes, but there have been comparatively few studies and discoveries in this area [38].

3.1. Development-Related Pheromones Secreted by Nematodes

The ability of nematodes to be so widely distributed in nature is closely related to their special developmental patterns. C. elegans lives freely in soil; it has a small, transparent body and serves as a model nematode species [39]. When the environment is suitable, an individual C. elegans starts to develop from a fertilized egg and progresses to adulthood through four stages of development. However, it stops feeding and developing if it encounters extreme conditions, such as a lack of food, elevated temperatures, or an increase in population density, and then the larva may enter a highly stress-resistant state called dauer diapause. This stage can last for several months. The nematodes eventually resume development and molt into the reproductive cycle under suitable conditions [40,41,42,43,44]. Much research shows that chemical pheromones can control dauer entry and exit [45,46].
The first dauer-inducing pheromone (daumone) was identified by means of the ethyl acetate extraction of a C. elegans liquid medium. The molecular structure of daumone was thereby determined to be (2)-(6R)-(3,5-dihydroxy-6-methyltetrahydropyran-2-yloxy) heptanoic acid, abbreviated to ascr#1 (also called daumone-1/ascaroside C7/asc-C7) (Table 1) [47]. Subsequently, ascr#2 (also called daumone-2/ascaroside C6) (Table 1) and ascr#3 (also called daumone-3/asc-∆C9/ascaroside C9) (Table 1) were also isolated and identified. Ascr#2 and ascr#3 induce dauer formation about 100 times more potently than ascr#1 does [48]. The pheromones that induce dauer formation may be single ascarosides or mixtures of different ascarosides, and these pheromones often act synergistically when mixed together. The dauer pheromone of C. elegans is mainly composed of ascr#2, ascr#3, and several other components, while in Caenorhabditis briggsae, the main component of the dauer pheromone is ascr#2 [49]. A derivative of ascr#2 has a β-glucosyl substituent linked to C2 of the ascarylose in ascr#2 and is named ascr#4 (also called daumone-4) (Table 1). The activity of ascr#4 is low [50]. Ascr#5 (also called daumone-5/ascaroside C3/asc-ωC3) (Table 1) is a potent dauer pheromone. The main function of ascr#5 is the activation of the axon regeneration pathway via SRG-36/SRG-37 GPCRs and EGL-30, indicating ascaroside signaling promotes axon regeneration by activating the GPCR-Gqα pathway [25]. In addition, the crh-1 gene plays an autonomous role in ascr#5, sensing ASI neurons in order to inhibit the dauer formation of C. elegans L2d [26]. Ascr#5 also produces synergistic effects with ascr#2 and ascr#3 [51]. In ASI neurons, ascaroside pheromones (compounds composed of ascr#2, ascr#3, and ascr#5) reversibly inhibit the expression of the str-3 chemical receptor gene, and when ascarosides are removed, its expression resumes. This process mainly occurs through the GPCR receptors SRBC-64/66 and SRG-36/37, which are required for str-3 repression [35]. Ascr#8 (Table 1) uniquely possesses a p-aminobenzoate group in its terminus; this group is a folate precursor that is derived from bacteria and is not synthesized by C. elegans [52].
Furthermore, Butcher et al. [53] used activity-guided fractionation and NMR to discover a structurally novel indole-3-carboxylic acid-modified ascaroside in C. elegans named icas#9 (also called indolecarboxyl ascaroside C5/ascaroside C5/IC-asc-C5) (Table 1). It can induce dauer development at low (nanomolar) concentrations yet is inhibited at higher concentrations. Nacq#1 (Table 1) also acts antagonistically with respect to dauer-inducing ascarosides. The N-acylated glutamine derivative nacq#1 is mainly found in the excretions of males and contains an uncommon triply unsaturated ten-carbon fatty acid. Nacq#1 signals that enough resources are available to finish the dauer stage and resume reproductive growth. Although it reduces lifespan, nacq#1 can antagonize diapause and accelerate development, hastening sexual maturation [54]. It also has a trans-isomer, nacq#2 (Table 1) [54], but its function is unknown.
P. pacificus is a model species that has been extensively studied in biology [55]. This nematode can enter the dauer stage or other stages if food is enough for growth [56,57]. Typically, the mouth of an adult that preys on other nematodes is more complex than that of a bacterivorous nematode. Pheromones can regulate the mouth dimorphism of P. pacificus [58]. Neelanjan et al. [59] analyzed fractions of the P. pacificus exo-metabolome and found that it has rich signaling molecules controlling adult phenotypic plasticity, including ascarosides ascr#1, 9, and 12. Pasc#9 (Table 1) was the most abundant derivative after pasc#1 and pasc#12. Pasc#9 comprises an N-succinyl 1-phenylethanolamide connected to ascarylose with a 4-hydroxypentanoic acid chain. Dasc#1 (Table 1) consists of two ascr#1 units; one ascr#1 unit is connected to carbon 4 of the other ascr#1 unit. A 3-ureido isobutyrate moiety is also present on carbon 4 of ubas#1 (Table 1), and ubas#1 also contains ascr#9 with the (ω)-oxygenated ascaroside oscr#9 connected at position 2 [59]. Recent studies revealed that the formation of ubas#1 and related metabolites specifically requires the putative carboxylesterase Ppa-uar-1 [60]. Additionally, dimeric ascarosides and ureido isobutyrate-substituted metabolites were first reported in P. pacificus. L-paratose forms the basis of part#9 (Table 1). Part#9 only differs from ascr#9 in terms of the stereochemistry of one hydroxyl group. Part#9 is also one of the components of npar#1. Npar#1 (Table 1) contains a derivative of the nucleoside adenosine. Although part#9, npar#1, ubas#1, and pasc#9 can induce dauer formation, npar#1 has a more intense effect than the others. Pasc#9, ascr#1, dasc#1, and npar#1 can induce eurystomatous mouth formation, a predatory morphology in the final larval and juvenile stages, in which P. pacificus-specific dasc#1 plays an important role [61,62].
Heterorhabditis bacteriophora is parasitic toward insects and has a developmental process similar to that of C. elegans. In the soil, the infective juveniles (IJs) survive as the only state of entomopathogenic nematodes. After IJs infect the host insects, they recover and lay eggs in their adults, which develop through four larval stages (J1–J4) to form the next generation [63,64,65]. In this process, H. bacteriophora secretes the ascaroside C11 ethanolamine (asc C11 EA) (Table 1), which prevents IJs from recovering to the J4 stage. Asc C11 EA comprises an ascarylose sugar, an ethanolamine fragment, and a carbon side chain containing ω-1 alcohol; the fatty-acid-derived portion of the side chain is 11 carbons long. Asc C11 EA and the dauer pheromone of C. elegans show structural similarity [66].
Figure 1 illustrates the schematic structure of the pheromones secreted by nematodes during their development. The figure presents a schematic diagram showing ascarylose sugars, variable-length fatty acids, and other moieties modifying them, which form different species of development-related ascaroside species.

3.2. Sex Pheromones Secreted by Nematodes

Mate selection is universal in sexually reproducing organisms, and pheromones provide individuals with advantageous mating information that helps them to select high-quality mates. In the twentieth century, the first sex pheromone was named bombykol, which is released from female silk moths (Bombyx mori) [67]. Sex pheromones have since been researched in more depth; they are defined as chemical substances produced by individuals that cause innate and rigid sexual behavior [68]. These pheromones have both sex- and species-specific effects. Nematode mating behavior is also regulated by pheromones [69]. Generally, the nematode mating response can be induced when the pheromone concentration is much lower than the concentration required for dauer formation.
C. elegans mainly reproduces as a hermaphrodite. However, most Caenorhabditis worm species achieve this by means of cross-fertilization. These hermaphrodites are essentially females with the ability to self-fertilize, and they can also mate with males, but their numbers are typically relatively low. Hermaphrodites do not appear to be attracted to male C. elegans, but males are attracted to them [70]. The ascarosides ascr#2, 3, 4, and 8 (Table 1) not only play roles in regulating nematode development but also function as sex pheromones that are known to attract males [71,72]. They show synergistic effects, whereby a mixture of ascr#2, 3, and 4 is an effective male attractant at low concentrations. Ascr#3 attracts C. elegans males but repels hermaphrodites and can increase the lifespan of C. elegans. Ascr#8 is a strong male-specific attractant and shows synergy with ascr#2 and ascr#3. A mixture composed of ascr#3 and ascr#8 strongly attracts males at ultra-low concentrations, but at higher concentrations, it is repulsive to hermaphrodites [36,50,71]. The other two ascarosides with sex pheromone functions, ascr#6.1 (Table 1) and ascr#6.2 (Table 1), were identified by Paul as diastereomeric side-chain-hydroxylated ascarosides [71]. Ascr#10 (also called asc-C9) (Table 1) makes up the majority of the sex-specific milieu of ascarosides produced by male C. elegans. Ascr#3 has an α, β-unsaturated fatty acid moiety, whereas ascr#10 has the corresponding dihydro-derivative; such minor structural modifications deeply influence their signaling properties. The male pheromone ascr#10 strongly attracts hermaphrodite nematodes and shortens their lifespan [73,74,75]. It also can increase germline proliferation and physiological cell death [76] and change the reproductive physiology of hermaphroditism, such as by improving sperm orientation and increasing the number of reproductive precursor cells in adults [77,78,79]. Furthermore, Dong et al. conducted a comparative analysis of indole ascaroside signaling for 14 Caenorhabditis species. Icas#2 and icas#6.2 (Table 1) were isolated from hermaphrodites of C. briggsae and were found to synergistically attract conspecific males [80].
Panagrellus redivivus has an ecological niche similar to that of C. elegans; it has a free-living lifestyle but belongs to a different clade. In contrast with C. elegans, the virgin females of P. redivivus attract and are attracted by the males, but they do not attract the same sex [81]. The ascaroside biosynthesis in P. redivivus is highly sex-specific. The females of P. redivivus can excrete ascr#1, ascr#10, and bhas#10 (Table 1) [74]. The males of P. redivivus can excrete dhas#18 (Table 1) [74]. Ascr#1 can strongly attract males, but high concentrations of ascr#1 repel the females of P. redivivus. At high concentrations, bhas#10 and ascr#10 attract males rather than females. Dhas#18, which is a known dihydroxy derivative of ascr#18 secreted by males as well as an ascaroside with extensive functionality as a characteristic of its lipid-derived side chain, can strongly attract the females of P. redivivus. Bhas#18 (Table 1) is a precursor for dhas#18 synthesis, but its exact function is unclear [74].
Rhabditis sp. SB347 is a unique free-living dioecious species that is often used in the laboratory [82]. The females of SB347 produce ascr#1 and ascr#9 (Table 1), which function as sex pheromones. At femtomolar levels, ascr#1 and ascr#9 are strongly attracted to males, but not to hermaphrodites and female nematodes [83].
In addition to the ascaroside pheromones, a different type of pheromone is secreted by Heterodera glycines, a plant nematode that is parasitic toward soybeans. The females secrete vanillic acid (Table 1), which also functions as a sex pheromone [38,84]. However, there are very few reports on non-ascarosides acting as pheromones in nematodes.
The female beet cyst nematode Heterodera schachtii can excrete a sex pheromone. The pheromone consists of at least two components, and the pheromone component is soluble in aqueous solutions with diethyl ether. These components may show superposition rather than synergy. However, their exact structure is unclear [85].
Bursaphelenchus xylophilus is a pine wood nematode (PWN). It has been shown that both sexes of B. xylophilus produce sex pheromones: unmated females attract conspecific males, and males attract both mated and unmated females through volatile chemical compounds. Additionally, Bursaphelenchus okinawaensis, which is associated with insect vectors and host plants, produces a pheromone that attracts males [86,87]. However, the exact composition of the compound is unknown.
The dimorphism of the adults is an important feature of the life history of Globodera rostochiensis. Hermaphrodites attract males for mating by producing pheromones. Four fractions of the homospecific sex pheromone produced by virgin females, which were isolated using chromatography technology, were tested for their ability to attract male G. rostochiensis; only two of the fractions showed sex pheromone activity. Several weakly basic polar compounds constitute the sex pheromone of G. rostochiensis. The exact structure of its components is unclear [88].
The chemical structure diagram for sex pheromones secreted by nematodes is shown in Figure 2. This diagram shows ascaroside building blocks associated with the mating of different species of nematodes, including ascarylose sugars, variable-length fatty acids, and other modification groups.

3.3. Aggregation of Pheromones Secreted by Nematodes

C. elegans uses specifically modified forms of the ascarosides that contain indole units as highly effective aggregation pheromones. The indole ascarosides (ICASs) incorporate an L-tryptophan-derived indole-3-carboxylic acid group, which is linked to the four-position of the ascarylose moiety. An indole carboxy unit forms one indole derivative, and it is connected to an ascarylose bearing a nine-carbon unsaturated side chain identical to that found in the known ascr#3; this indole carboxy ascaroside is called “icas#3” (Table 1). The icas#3 occurs primarily by means of an expression protein in C. elegans, CEST-3, adding an IC group to the corresponding unmodified ascr#3 [15,89]. Icas#3 and icas#9 are relatively good attractants [15]. The 4-hydroxybenzoyl derivative of ascr#3 is called hbas#3 (Table 1). Hbas#3 was the first ascaroside with a 4-hydroxybenzoyl structure to be discovered. Hbas#3 strongly attracts C. elegans at low concentrations (10 fM), more effectively so than icas#3 and icas#9 [19]. Ascr#5 in combination with ascr#2 or ascr#3 may influence the aggregation of C. elegans adults; however, more in-depth research is needed on this topic [90].
See Figure 3 for a schematic overview of nematode pheromones related to their aggregation. It illustrates ascarylose sugars, fatty acids with variable lengths, and other modifications that form aggregation-related ascaroside species.

3.4. Pheromones with Other Functions Secreted by Nematodes

The L1 larvae of C. elegans can specifically produce certain octopamine ascarosides, in which the ascarylose four-position is linked to a side chain derived from the succinylation of the neurotransmitter octopamine. The octopamine ascarosides osas#2 (Table 1), osas#9 (Table 1), and osas#10 (Table 1) play roles in dispersal [72]. Osas#9 is a pheromone that acts as a dispersal signal, especially in the case of a lack of food. Avoidance reactions to osas#9 require the G-protein-coupled receptor TYRA-2 [34]. With a continuous decrease in food, ascr#10 and osas#10 are converted to ascr#9, osas#9, and icas#9 [72].
Ascr#3 was found to regulate metabolism and avoidance behavior, it was defined as a population density pheromone. When food is scarce, ascr#3 causes hermaphrodites to have an avoidance effect [91,92,93]. When the ADF of a single sensory neuron is removed, both sexes are weakly rejected by the ascaroside ascr#3. Although ADF has functions in both sexes, ascr#3 is only detected in males, which is the result of the main sex regulator tra-1 [94]. A derivative of ascr#3 called mbas#3 (Table 1) was the first ascaroside discovered to have an (E)-2-methyl-2-butenoyl structure [19], and it acts as a dispersal signal in C. elegans, as well as having an antagonistic effect on the attractant characteristics of indole ascarides such as icas#3 and icas#9 [95].
C. elegans exist in two states (roaming and dwelling) when searching for bacterial food [96]. At physiological levels, some ascarosides regulate foraging by inhibiting roaming behavior. Ascr#2, 3, 5, and 8 and icas#9 have some effects on the foraging behavior of C. elegans. Different strains of C. elegans have different sensitivities to icas#9 due to differences in the expression of the srx-43 gene, which encodes the icas#9 receptor [97]. C. elegans can also develop certain memory behaviors in response to pheromones such as ascr#3, ascr#5, and icas#9 [98,99,100].
Research has shown that abundant ascr#18 (Table 1) could be isolated from Meloidogyne incognita, Meloidogyne javanica, and Meloidogyne hapla, as well as from cyst (H. glycines) and lesion (Pratylenchus brachyurus) nematodes [101]. This compound is also present in C. elegans [19] and entomopathogenic nematodes [36,66]. Ascr#18 can be sensed by a wide range of plant species, which in turn mount defense responses against nematodes.
Entomopathogenic nematode IJs can sense ascaroside mixtures including ascr#2, 3, and 8 and icas#9 from C. elegans, which causes the dispersal of nematodes. The pheromone mixture of ascr#9 and ascr#11 (Table 1) from consumed insect host corpses contributes to IJ dispersal [102,103]. Ascr#9 and ascr#11 are structural analogs, and they can be interchanged in the mixture of dispersal pheromones. Ascr#9 was detected in some species of Steinernema spp. (S. feltiae, S. carpocapsae, S. riobrave, and S. diaprepesi) and Heterorhabditis spp. (H. zealandica, H. floridensis, and H. bacteriophora), which indicated that ascr#9 may be widely present in dispersal mixtures from entomopathogenic nematodes. However, it was found that ascr#11 is present in some species of Steinernema, but not in Heterorhabditis, indicating that ascr#11 may be specific to Steinernema [103,104]. Ascr#12 (Table 1) can induce the IJs recovery of H. bacteriophora H06 [105], while ascr#11 can enhance the IJ yields of Steinernema carpocapsae All and H. bacteriophora H06 in the liquid medium [106].
The pinewood nematode, B. xylophilus, the causal agent for pine wilt disease and a global quarantine pest, usually displaces Bursaphelenchus mucronatus, a native sympatric sibling species. Similar to what occurs in C. elegans, in B. xylophilus, the pheromones comprise the hydrophilic ascarosides family, which are derivatives of 3,6-dideoxy-L-saccharose linked to fatty-acid-derived side chains; they regulate the transmission of B. xylophilus and its vector beetle, and they regulate the lifecycle of B. xylophilus [107]. Ascr#9 is the major component of the ascarosides of the two nematodes; it not only increases the number of invasive strains but also reduces the number of native strains [108]. Moreover, ascr#9 plays a leading role in pheromone-regulated reproductive plasticity. At the molecular level, two genes, Bxydaf-38 and Bxysrd-10, participate in the perception of ascr#9 [108]. When mixed with ascr#12, it acts synergistically and also increases body length in the females of B. xylophilus, though it reduces body length in B. mucronatus [109].
C. elegans has shown a tendency to be attracted to a series of odorous substances, and with the passage of time, this tendency changes from attraction to dispersal [98,99]. This varied pheromone-mediated behavior is called olfactory plasticity, which depends on the population density [110]. However, the pheromone component that plays a major role in this process has not been identified. In addition, the pheromones released by injured conspecific nematodes are repellent to nematodes, and they may contain alarm pheromones. These alarm pheromones may not belong to the ascaroside class of pheromones [111]. However, their exact structure has not been identified.
The following Figure 4 displays the chemical structure diagram of function pheromones secreted by nematodes. This diagram shows the ascaroside building blocks associated with the functions of different species of nematodes, including ascarylose sugars, variable-length fatty acids, and other modification groups.
Table
Table 1. Pheromones are secreted by nematodes.
Table 1. Pheromones are secreted by nematodes.
NameChemical ConstitutionFunctionOrganismReference
Asc C11 EAMolecules 28 02409 i001Development C. elegans[66]
Ascr#1Molecules 28 02409 i002Development; matingC. elegans, P. pacificus, P. redivivus, and Rhabditis sp. SB347[47,48,59,61,62,74,83]
Ascr#2Molecules 28 02409 i003Development, mating, foraging, and dispersalC. elegans; C. briggsae[48,49,71,72,97]
Ascr#3Molecules 28 02409 i004Development, mating, foraging, and dispersalC. elegans[36,48,49,50,71,72,90,91,92,93,95,97,98,99,100]
Ascr#4Molecules 28 02409 i005Development; matingC. elegans[50,71,72]
Ascr#5Molecules 28 02409 i006Development; foragingC. elegans[25,26,51,97]
Ascr#6.1Molecules 28 02409 i007MatingC. elegans[71]
Ascr#6.2Molecules 28 02409 i008MatingC. elegans[71]
Ascr#8Molecules 28 02409 i009Development, mating, foraging, and dispersalC. elegans[36,50,52,71,72,97]
Ascr#9Molecules 28 02409 i010Mating; dispersalC. elegans, P. pacificus, Rhabditis sp. SB347, B. xylophilus, B. mucronatus
H. bacteriophora, H.zealandica, H. floridensis, S. carpocapsae, S. riobrave, S. diaprepesi, and S. feltiae		[36,59,83,102,103,104,108,109]
Ascr#10Molecules 28 02409 i011MatingC. elegans; P. redivivus[73,74,75,76,77,78,79,101]
Ascr#11Molecules 28 02409 i012DispersalC. elegans, S. carpocapsae, S. riobrave, S. diaprepesi, and S. feltiae[102,103,104,106]
Ascr#12Molecules 28 02409 i013DevelopmentC. elegans; P. pacificus[36,59,105,109]
Ascr#18Molecules 28 02409 i014AvoidanceM. incognita, M. javanica, M. hapla, H. glycines, and P. brachyurus[19,101]
Bhas#10Molecules 28 02409 i015MatingC. elegans; P. redivivus[74]
Bhas#18Molecules 28 02409 i016UnknownP. redivivus[74]
Dasc#1Molecules 28 02409 i017DevelopmentP. pacificus[59,61,62]
Dhas#18Molecules 28 02409 i018MatingP. redivivus[74]
Hbas#3Molecules 28 02409 i019AggregationC. elegans[19]
Icas#2Molecules 28 02409 i020MatingC. briggsae[80]
Icas#3Molecules 28 02409 i021AggregationC. elegans[15,89]
Icas#6.2 Molecules 28 02409 i022MatingC. briggsae[80]
Icas#9Molecules 28 02409 i023Development, aggregation, foraging, and dispersal C. elegans[15,19,53,72,97]
Mbas#3Molecules 28 02409 i024DispersalC. elegans[19,95]
Nacq#1Molecules 28 02409 i025DevelopmentC. elegans[54]
Nacq#2Molecules 28 02409 i026UnknownC. elegans[54]
Npar#1Molecules 28 02409 i027DevelopmentP. pacificus[59,61,62]
Osas#2Molecules 28 02409 i028R=(C=O)CH3DispersalC. elegans[72]
Osas#10R=(CH2)4COOHDispersalC. elegans[72]
Osas#9Molecules 28 02409 i029DispersalC. elegans[34,72]
Part#9Molecules 28 02409 i030DevelopmentP. pacificus[59,61,62]
Pasc#9Molecules 28 02409 i031DevelopmentP. pacificus[59,61,62]
Ubas#1Molecules 28 02409 i032DevelopmentP. pacificus[59,61,62]
Vanillic acidMolecules 28 02409 i033MatingH. glycines[38,84]

4. Nematode Pheromone Communication with Other Species

Nematode pheromones mainly function in an intraspecies manner, but further research has shown that they also function between different species [16,112], such as fungi, plants, and insects.
Manosalva et al. [101] found that nematode pheromones trigger defense responses in different organs of plants. Moreover, plants can metabolize nematode pheromones via peroxisomal β-oxidation and thus alter their chemical information, and they can produce a blend of ascarosides to control plant nematodes and reduce harm to themselves [13]. Interestingly, increased callose buildup was seen in Arabidopsis leaves after treatment with ascr#1 and ascr#18. AOS, PR1, PDF1.2, LOX2, and other defense-related genes also increased their expression as a result of ascr#18, which may have contributed to the improved plant defensive responses [113].
The typical pine wilt disease encompasses complex associations between PWN, symbiotic fungi, and vector beetles. In this system, nematode pheromones not only increase the number of mycelia, and improve the spread of fungi and nematode efficiency [114], but they also promote the pupation of beetles by inducing them to produce the molting hormone and upregulating the expression of genes related to the molting hormone [107]. Interestingly, PWN vector beetles can also produce ascarosides that promote the aggregation of their symbiotic plant-parasitic nematode species [107]. This indicates that nematode pheromones can regulate interspecific interactions.
Nematode-trapping fungi are predators that can consume nematodes and are widespread in soils of distinct ecological provenances [115]. Nematode-trapping fungi can detect and respond to nematode pheromones for the generation of trapping devices to catch and consume nematodes. For instance, the model Arthrobotrys oligospora can form traps of adhesive nets via the stimulation of ascaroside pheromones [27,116,117].
Recent studies have revealed that the main components of nematode pheromones, ascarosides, can be widely metabolized by animals, plants, and microorganisms [112], which may interact with certain nematodes by manipulating ascaroside signaling. The responses of other species to nematode pheromones may accelerate the rapid evolution of pheromones and may provide evidence for the synergistic evolution of species.

5. Conclusions

Pheromones play wide-ranging roles in nematodes, such as in their development, mating, aggregation, olfactory plasticity, and dispersal. These pheromones are closely related to a variety of factors such as lifestyle, sex, and developmental stage, and the nematodes living in various habitats can produce rich and diverse pheromones (Figure 5) [118,119]. C. elegans can secrete many kinds of ascarosides to improve development and induce dauer formation. Entomopathogenic nematodes can secrete different ascarosides to assist them in finding hosts and thriving. The pheromones secreted by plant parasitic nematodes are even closely related to interspecific competition. The latest research has shown that secreted pheromones can be sensed and even metabolized by organisms in the environment (such as animals, plants, and microorganisms).
Ascarosides are major components of nematode pheromones; they are highly conserved and species-specific, and the same ascarosides may play different roles among nematodes. In chemical terms, they comprise dideoxysugar ascaryloses linked to different fatty-acid side chains along with derivatives of amino acids, folate, and other primary metabolites. Structural and functional diversity exists due to differences in the lengths of the side chains and the derivatives. The effects of ascarosides on nematodes are not only highly dependent on their chemical structure but are also linked to their concentrations and the synergistic effects that take place between ascarosides [120,121].
Approximately 200 ascarosides have been discovered and identified from over 20 different nematodes [17,42,113]. The functions of most of them are unknown, whereas a few have been found to function as pheromones [19,122]. There are large interspecific differences in the structures and compositions of ascarosides [36]. However, these ascarosides and the C. elegans ascarosides share some structural similarities. For example, the ascarosides produced by Ascaris suum have long chain structures, similar to those of the ascarosides produced by C. elegans [71]. Recent research has shown that the nematode C. briggsae biosynthesizes ascarosides in a manner similar to C. elegans and also has a related developmental pathway that induces the stress-resistant dauer life stage. Thus, ascarosides may play similar roles in other nematode species compared with C. elegans. Studying the functions of ascarosides could provide a new method for controlling the parasitic nematodes, but they need to be further explored. Moreover, nematode pheromones have effects on other species, but the current study has revealed only the tip of the iceberg of the complex multidirectional communication network mediated by ascarosides. Therefore, it is important to further investigate the responses of other species to ascarosides, particularly regarding pathways and receptors, in order to explain this process.
The discovery of nematode pheromones provides new experimental channels for studying pheromone communication and its evolution, which will have significant value in the biological control of harmful parasitic nematodes and chemical ecology. A systematic description of the structures and functions of the pheromones of C. elegans and other nematodes will be helpful in improving our understanding of various biological processes. Although nematode pheromones are only a small class of compounds to be studied in depth, they will certainly have a major impact on the study of interspecific and intraspecific interactions in nematodes.

Author Contributions

Conceptualization, X.Z. and X.W.; writing—original draft preparation, B.Y. and J.W.; writing—review and editing, X.W.; J.W. and B.Y.; supervision, X.W. and X.Z. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (32260029), the Applied Basic Research Foundation of Yunnan Province (202101AT070266), and the Postgraduate Research and Innovation Foundation of Yunnan University (KC-22221839).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. van den Hoogen, J.; Geisen, S.; Wall, D.H.; Wardle, D.A.; Traunspurger, W.; de Goede, R.G.; Adams, B.J.; Ahmad, W.; Ferris, H.; Bardgett, R.D.; et al. A Global Database of Soil Nematode Abundance and Functional Group Composition. Sci. Data 2020, 7, 103. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Abad, P.; Gouzy, J.; Aury, J.M.; Castagnone-Sereno, P.; Danchin, E.G.; Deleury, E.; Perfus-Barbeoch, L.; Anthouard, V.; Artiguenave, F.; Blok, V.C.; et al. Genome Sequence of the Metazoan Plant-Parasitic Nematode Meloidogyne incognita. Nat. Biotechnol. 2008, 26, 909–915. [Google Scholar] [CrossRef] [Green Version]
  3. Kenney, E.; Eleftherianos, I. Entomopathogenic and Plant Pathogenic Nematodes as Opposing Forces in Agriculture. Int. J. Parasitol. 2016, 46, 13–19. [Google Scholar] [CrossRef] [Green Version]
  4. Luporini, P.; Pedrini, B.; Alimenti, C.; Vallesi, A. Revisiting Fifty Years of Research on Pheromone Signaling in Ciliates. Eur. J. Protistol. 2016, 55, 26–38. [Google Scholar] [CrossRef] [PubMed]
  5. Alimenti, C.; Buonanno, F.; Di Giuseppe, G.; Guella, G.; Luporini, P.; Ortenzi, C.; Vallesi, A. Bioactive Molecules from Ciliates: Structure, Activity, and Applicative Potential. J. Eukaryot. Microbiol. 2022, 69, e12887. [Google Scholar] [CrossRef] [PubMed]
  6. Jiang, N.J.; Mo, B.T.; Guo, H.; Yang, J.; Tang, R.; Wang, C.Z. Revisiting the Sex Pheromone of the Fall Armyworm Spodoptera frugiperda, a New Invasive Pest in South China. Insect Sci. 2022, 29, 865–878. [Google Scholar] [CrossRef]
  7. Chen, D.; Hou, L.; Wei, J.; Guo, S.; Cui, W.; Yang, P.; Kang, L.; Wang, X. Aggregation Pheromone 4-Vinylanisole Promotes the Synchrony of Sexual Maturation in Female Locusts. Elife 2022, 11, e74581. [Google Scholar] [CrossRef]
  8. Tamrakar, S.; Huerta, B.; Chung-Davidson, Y.-W.; Li, W. Plasma Metabolomic Profiles Reveal Sex- and Maturation-Dependent Metabolic Strategies in Sea Lamprey (Petromyzon marinus). Metabolomics 2022, 18, 90. [Google Scholar] [CrossRef]
  9. Zaremska, V.; Renzone, G.; Arena, S.; Ciaravolo, V.; Buberl, A.; Balfanz, F.; Scaloni, A.; Knoll, W.; Pelosi, P. An Odorant-Binding Protein in the Elephant’s Trunk is Finely Tuned to Sex Pheromone (Z)-7-Dodecenyl Acetate. Sci. Rep. 2022, 12, 19982. [Google Scholar] [CrossRef]
  10. Mayer, M.G.; Rödelsperger, C.; Witte, H.; Riebesell, M.; Sommer, R.J. The Orphan Gene Dauerless Regulates Dauer Development and Intraspecific Competition in Nematodes by Copy Number Variation. PLoS Genet. 2015, 11, e1005146. [Google Scholar] [CrossRef] [Green Version]
  11. Edison, A.S. Caenorhabditis elegans Pheromones Regulate Multiple Complex Behaviors. Curr. Opin. Neurobiol. 2009, 19, 378–388. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. von Reuss, S.H.; Schroeder, F.C. Combinatorial Chemistry in Nematodes: Modular Assembly of Primary Metabolism-Derived Building Blocks. Nat. Prod. Rep. 2015, 32, 994–1006. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Manohar, M.; Tenjo-Castano, F.; Chen, S.; Zhang, Y.K.; Kumari, A.; Williamson, V.M.; Wang, X.; Klessig, D.F.; Schroeder, F.C. Plant Metabolism of Nematode Pheromones Mediates Plant-Nematode Interactions. Nat. Commun. 2020, 11, 208. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Ludewig, A.H.; Schroeder, F.C. Ascaroside Signaling in C. elegans. WormBook 2013, 18, 1–22. [Google Scholar] [CrossRef] [Green Version]
  15. Srinivasan, J.; von Reuss, S.H.; Bose, N.; Zaslaver, A.; Mahanti, P.; Ho, M.C.; O’Doherty, O.G.; Edison, A.S.; Sternberg, P.W.; Schroeder, F.C. A Modular Library of Small Molecule Signals Regulates Social Behaviors in Caenorhabditis elegans. PLoS Biol. 2012, 10, e1001237. [Google Scholar] [CrossRef] [Green Version]
  16. McGrath, P.T.; Ruvinsky, I. A Primer on Pheromone Signaling in Caenorhabditis elegans for Systems Biologists. Curr. Opin. Syst. Biol. 2019, 13, 23–30. [Google Scholar] [CrossRef]
  17. Park, J.Y.; Joo, H.J.; Park, S.; Paik, Y.K. Ascaroside Pheromones: Chemical Biology and Pleiotropic Neuronal Functions. Int. J. Mol. Sci. 2019, 20, 3898. [Google Scholar] [CrossRef] [Green Version]
  18. Wang, J.; Kim, S.K. Global Analysis of Dauer Gene Expression in Caenorhabditis elegans. Development 2003, 130, 1621–1634. [Google Scholar] [CrossRef] [Green Version]
  19. von Reuss, S.H.; Bose, N.; Srinivasan, J.; Yim, J.J.; Judkins, J.C.; Sternberg, P.W.; Schroeder, F.C. Comparative Metabolomics Reveals Biogenesis of Ascarosides, a Modular Library of Small-Molecule Signals in C. elegans. J. Am. Chem. Soc. 2012, 134, 1817–1824. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  20. Shi, H.; Huang, X.; Chen, X.; Yang, Y.; Wang, Z.; Yang, Y.; Wu, F.; Zhou, J.; Yao, C.; Ma, G.; et al. Acyl-CoA Oxidase ACOX-1 Interacts with a Peroxin PEX-5 to Play Roles in Larval Development of Haemonchus contortus. PLoS Pathog. 2021, 17, e1009767. [Google Scholar] [CrossRef]
  21. Gao, C.; Li, Q.; Yu, J.; Li, S.; Cui, Q.; Hu, X.; Chen, L.; Zhang, S.O. Endocrine Pheromones Couple Fat Rationing to Dauer Diapause Through HNF4α Nuclear Receptors. Sci. China Life Sci. 2021, 64, 2153–2174. [Google Scholar] [CrossRef] [PubMed]
  22. Ding, H.; Shi, H.; Shi, Y.; Guo, X.; Zheng, X.; Chen, X.; Zhou, Q.; Yang, Y.; Du, A. Characterization and Function Analysis of a Novel Gene, Hc-maoc-1, in the Parasitic Nematode Haemonochus contortus. Parasit. Vectors 2017, 10, 67. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Markov, G.V.; Meyer, J.M.; Panda, O.; Artyukhin, A.B.; Claassen, M.; Witte, H.; Schroeder, F.C.; Sommer, R.J. Functional Conservation and Divergence of daf-22 Paralogs in Pristionchus pacificus Dauer Development. Mol. Biol. Evol. 2016, 33, 2506–2514. [Google Scholar] [CrossRef] [Green Version]
  24. Rebecca, A.; Butchera, J.R.R.; Weiqing, L.; Ruvkunc, J.C.G.; Mak, H.Y. Biosynthesis of the Caenorhabditis elegans Dauer Pheromone. Proc. Natl. Acad. Sci. USA 2009, 106, 1875–1879. [Google Scholar]
  25. Shimizu, T.; Sugiura, K.; Sakai, Y.; Dar, A.R.; Butcher, R.A.; Matsumoto, K.; Hisamoto, N. Chemical Signaling Regulates Axon Regeneration via the GPCR-Gqα Pathway in Caenorhabditis elegans. J. Neurosci. 2022, 42, 720–730. [Google Scholar] [CrossRef]
  26. Park, J.; Oh, H.; Kim, D.Y.; Cheon, Y.; Park, Y.J.; Hwang, H.; Neal, S.J.; Dar, A.R.; Butcher, R.A.; Sengupta, P.; et al. CREB Mediates the C. elegans Dauer Polyphenism Through Direct and Cell-Autonomous Regulation of TGF-β Expression. PLoS Genet. 2021, 17, e1009678. [Google Scholar] [CrossRef]
  27. Chen, S.A.; Lin, H.C.; Schroeder, F.C.; Hsueh, Y.P. Prey Sensing and Response in a Nematode-Trapping Fungus is Governed by the MAPK Pheromone Response Pathway. Genetics 2021, 217, iyaa008. [Google Scholar] [CrossRef] [PubMed]
  28. Ilbay, O.; Ambros, V. Pheromones and Nutritional Signals Regulate the Developmental Reliance on let-7 Family MicroRNAs in C. elegans. Curr. Biol. 2019, 29, 1735–1745.e4. [Google Scholar] [CrossRef]
  29. Chute, C.D.; Srinivasan, J. Chemical Mating Cues in C. elegans. Semin. Cell Dev. Biol. 2014, 33, 18–24. [Google Scholar] [CrossRef]
  30. Cheon, Y.; Hwang, H.; Kim, K. Plasticity of Pheromone-Mediated Avoidance Behavior in C. elegans. J. Neurogenet. 2020, 34, 420–426. [Google Scholar] [CrossRef]
  31. Wu, T.; Ge, M.; Wu, M.; Duan, F.; Liang, J.; Chen, M.; Gracida, X.; Liu, H.; Yang, W.; Dar, A.R.; et al. Pathogenic Bacteria Modulate Pheromone Response to Promote Mating. Nature 2023, 613, 324–331. [Google Scholar] [CrossRef] [PubMed]
  32. Aprison EZ, R.I. The Roles of Several Sensory Neurons and the Feedback From Egg Laying in Regulating the Germline Response to a Sex Pheromone in C. elegans Hermaphrodites. MicroPubl. Biol. 2022, 2022, 523. [Google Scholar] [CrossRef]
  33. Wong, S.S.; Yu, J.; Schroeder, F.C.; Kim, D.H. Population Density Modulates the Duration of Reproduction of C. elegans. Curr. Biol. 2020, 30, 2602–2607.e2. [Google Scholar] [CrossRef] [PubMed]
  34. Chute, C.D.; DiLoreto, E.M.; Zhang, Y.K.; Reilly, D.K.; Rayes, D.; Coyle, V.L.; Choi, H.J.; Alkema, M.J.; Schroeder, F.C.; Srinivasan, J. Co-option of Neurotransmitter Signaling for Inter-Organismal Communication in C. elegans. Nat. Commun. 2019, 10, 3186. [Google Scholar] [CrossRef] [Green Version]
  35. Park, J.; Choi, W.; Dar, A.R.; Butcher, R.A.; Kim, K. Neuropeptide Signaling Regulates Pheromone-Mediated Gene Expression of a Chemoreceptor Gene in C. elegans. Mol. Cells 2019, 42, 28–35. [Google Scholar] [CrossRef] [PubMed]
  36. Choe, A.; von Reuss, S.H.; Kogan, D.; Gasser, R.B.; Platzer, E.G.; Schroeder, F.C.; Sternberg, P.W. Ascaroside Signaling is Widely Conserved Among Nematodes. Curr. Biol. 2012, 22, 772–780. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Lee, D.; Fox, B.W.; Palomino, D.C.F.; Panda, O.; Tenjo, F.J.; Koury, E.J.; Evans, K.S.; Stevens, L.; Rodrigues, P.R.; Kolodziej, A.R.; et al. Natural Genetic Variation in the Pheromone Production of C. elegans. bioRxiv 2022. [Google Scholar] [CrossRef]
  38. Jaffe, H.; Huettel, R.N.; Demilo, A.B.; Hayes, D.K.; Rebois, R.V. Isolation and Identification of a Compound from Soybean Cyst Nematode, Heterodera glycines, with Sex Pheromone Activity. J. Chem. Ecol. 1989, 15, 2031–2043. [Google Scholar] [CrossRef]
  39. Kaletta, T.; Hengartner, M.O. Finding Function in Novel Targets: C. elegans as a Model Organism. Nat. Rev. Drug Discov. 2006, 5, 387–398. [Google Scholar] [CrossRef]
  40. Cassada, R.C.; Russell, R.L. The Dauerlarva, A Post-Embryonic Developmental Variant of the Nematode Caenorhabditis elegans. Dev. Biol. 1975, 46, 326–342. [Google Scholar] [CrossRef]
  41. Klass, M.; Hirsh, D. Non-Ageing Developmental Variant of Caenorhabditis elegans. Nature 1976, 260, 523–525. [Google Scholar] [CrossRef] [PubMed]
  42. Schroeder, F.C. Modular Assembly of Primary Metabolic Building Blocks: A Chemical Language in C. elegans. Chem. Biol. 2015, 22, 7–16. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Riddle, D.L.; Albert, P.S. Genetic and Environmental Regulation of Dauer Larva Development. In C. elegans II; Riddle, D.L., Blumenthal, T., Meyer, B.J., Priess, J.R., Eds.; Cold Spring Harbor Laboratory Press Copyright © 2023; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, USA, 1997. [Google Scholar]
  44. Kim, S.; Paik, Y.K. Developmental and Reproductive Consequences of Prolonged Non-aging Dauer in Caenorhabditis elegans. Biochem. Biophys. Res. Commun. 2008, 368, 588–592. [Google Scholar] [CrossRef] [PubMed]
  45. Golden, J.W.; Riddle, D.L. A Pheromone Influences Larval Development in the Nematode Caenorhabditis elegans. Science 1982, 218, 578–580. [Google Scholar] [CrossRef]
  46. Butcher, R.A. Small-Molecule Pheromones and Hormones Controlling Nematode Development. Nat. Chem. Biol. 2017, 13, 577–586. [Google Scholar] [CrossRef]
  47. Jeong, P.Y.; Jung, M.; Yim, Y.H.; Kim, H.; Park, M.; Hong, E.; Lee, W.; Kim, Y.H.; Kim, K.; Paik, Y.K. Chemical Structure and Biological Activity of the Caenorhabditis elegans Dauer-Inducing Pheromone. Nature 2005, 433, 541–545. [Google Scholar] [CrossRef]
  48. Butcher, R.A.; Fujita, M.; Schroeder, F.C.; Clardy, J. Small-Molecule Pheromones that Control Dauer Development in Caenorhabditis elegans. Nat. Chem. Biol. 2007, 3, 420–422. [Google Scholar] [CrossRef]
  49. Cohen, S.M.; Wrobel, C.J.J.; Prakash, S.J.; Schroeder, F.C.; Sternberg, P.W. Formation and Function of Dauer Ascarosides in the Nematodes Caenorhabditis briggsae and Caenorhabditis elegans. G3 Bethesda 2022, 12, jkac014. [Google Scholar] [CrossRef]
  50. Srinivasan, J.; Kaplan, F.; Ajredini, R.; Zachariah, C.; Alborn, H.T.; Teal, P.E.; Malik, R.U.; Edison, A.S.; Sternberg, P.W.; Schroeder, F.C. A Blend of Small Molecules Regulates Both Mating and Development in Caenorhabditis elegans. Nature 2008, 454, 1115–1118. [Google Scholar] [CrossRef] [Green Version]
  51. Butcher, R.A.; Ragains, J.R.; Kim, E.; Clardy, J. A Potent Dauer Pheromone Component in Caenorhabditis elegans that Acts Synergistically with Other Components. Proc. Natl. Acad. Sci. USA 2008, 105, 14288–14292. [Google Scholar] [CrossRef] [Green Version]
  52. Reilly, D.K.; McGlame, E.J.; Vandewyer, E.; Robidoux, A.N.; Muirhead, C.S.; Northcott, H.T.; Joyce, W.; Alkema, M.J.; Gegear, R.J.; Beets, I.; et al. Distinct Neuropeptide-Receptor Modules Regulate a Sex-Specific Behavioral Response to a Pheromone. Commun. Biol. 2021, 4, 1018. [Google Scholar] [CrossRef] [PubMed]
  53. Butcher, R.A.; Ragains, J.R.; Clardy, J. An Indole-Containing Dauer Pheromone Component with Unusual Dauer Inhibitory Activity at Higher Concentrations. Org. Lett. 2009, 11, 3100–3103. [Google Scholar] [CrossRef] [PubMed]
  54. Ludewig, A.H.; Artyukhin, A.B.; Aprison, E.Z.; Rodrigues, P.R.; Pulido, D.C.; Burkhardt, R.N.; Panda, O.; Zhang, Y.K.; Gudibanda, P.; Ruvinsky, I.; et al. An Excreted Small Molecule Promotes C. elegans Reproductive Development and Aging. Nat. Chem. Biol. 2019, 15, 838–845. [Google Scholar] [CrossRef] [PubMed]
  55. Hong, R.L.; Sommer, R.J. Pristionchus pacificus: A Well-Rounded Nematode. BioEssays 2006, 28, 651–659. [Google Scholar] [CrossRef]
  56. Meyer, J.M.; Baskaran, P.; Quast, C.; Susoy, V.; Rödelsperger, C.; Glöckner, F.O.; Sommer, R.J. Succession and Dynamics of Pristionchus Nematodes and Their Microbiome During Decomposition of Oryctes Borbonicus on La Réunion Island. Environ. Microbiol. 2017, 19, 1476–1489. [Google Scholar] [CrossRef] [PubMed]
  57. Sommer, R.J.; McGaughran, A. The Nematode Pristionchus pacificus as a Model System for Integrative Studies in Evolutionary Biology. Mol. Ecol. 2013, 22, 2380–2393. [Google Scholar] [CrossRef]
  58. Bento, G.; Ogawa, A.; Sommer, R.J. Co-Option of the Hormone-Signalling Module Dafachronic Acid-DAF-12 in Nematode Evolution. Nature 2010, 466, 494–497. [Google Scholar] [CrossRef]
  59. Bose, N.; Ogawa, A.; von Reuss, S.H.; Yim, J.J.; Ragsdale, E.J.; Sommer, R.J.; Schroeder, F.C. Complex Small-Molecule Architectures Regulate Phenotypic Plasticity in a Nematode. Angew. Chem. Int. Ed. Engl. 2012, 51, 12438–12443. [Google Scholar] [CrossRef] [Green Version]
  60. Falcke, J.M.; Bose, N.; Artyukhin, A.B.; Rödelsperger, C.; Markov, G.V.; Yim, J.J.; Grimm, D.; Claassen, M.H.; Panda, O.; Baccile, J.A.; et al. Linking Genomic and Metabolomic Natural Variation Uncovers Nematode Pheromone Biosynthesis. Cell Chem. Biol. 2018, 25, 787–796.e12. [Google Scholar] [CrossRef] [Green Version]
  61. Bose, N.; Meyer, J.M.; Yim, J.J.; Mayer, M.G.; Markov, G.V.; Ogawa, A.; Schroeder, F.C.; Sommer, R.J. Natural Variation in Dauer Pheromone Production and Sensing Supports Intraspecific Competition in Nematodes. Curr. Biol. 2014, 24, 1536–1541. [Google Scholar] [CrossRef] [Green Version]
  62. Werner, M.S.; Claaßen, M.H.; Renahan, T.; Dardiry, M.; Sommer, R.J. Adult Influence on Juvenile Phenotypes by Stage-Specific Pheromone Production. iScience 2018, 10, 123–134. [Google Scholar] [CrossRef] [PubMed]
  63. Ciche, T.A.; Kim, K.S.; Kaufmann-Daszczuk, B.; Nguyen, K.C.; Hall, D.H. Cell Invasion and Matricide during Photorhabdus luminescens Transmission by Heterorhabditis bacteriophora Nematodes. Appl. Environ. Microbiol. 2008, 74, 2275–2287. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Ciche, T.A.; Ensign, J.C. For the Insect Pathogen Photorhabdus luminescens, Which End of a Nematode is Out? Appl. Environ. Microbiol. 2003, 69, 1890–1897. [Google Scholar] [CrossRef] [Green Version]
  65. Ciche, T. The Biology and Genome of Heterorhabditis bacteriophora. WormBook 2007, 20, 1–9. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  66. Noguez, J.H.; Conner, E.S.; Zhou, Y.; Ciche, T.A.; Ragains, J.R.; Butcher, R.A. A Novel Ascaroside Controls the Parasitic Life Cycle of the Entomopathogenic Nematode Heterorhabditis bacteriophora. ACS Chem. Biol. 2012, 7, 961–966. [Google Scholar] [CrossRef] [Green Version]
  67. Gomez-Diaz, C.; Benton, R. The Joy of Sex Pheromones. EMBO Rep. 2013, 14, 874–883. [Google Scholar] [CrossRef] [Green Version]
  68. Wyatt, T.D. Pheromones and Signature Mixtures: Defining Species-Wide Signals and Variable Cues for Identity in Both Invertebrates and Vertebrates. J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 2010, 196, 685–700. [Google Scholar] [CrossRef] [PubMed]
  69. Leighton, D.H.; Sternberg, P.W. Mating Pheromones of Nematoda: Olfactory Signaling with Physiological Consequences. Curr. Opin. Neurobiol. 2016, 38, 119–124. [Google Scholar] [CrossRef]
  70. Simon, J.M.; Sternberg, P.W. Evidence of a Mate-Finding Cue in the Hermaphrodite Nematode Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2002, 99, 1598–1603. [Google Scholar] [CrossRef] [Green Version]
  71. Pungaliya, C.; Srinivasan, J.; Fox, B.W.; Malik, R.U.; Ludewig, A.H.; Sternberg, P.W.; Schroeder, F.C. A Shortcut to Identifying Small Molecule Signals that Regulate Behavior and Development in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2009, 106, 7708–7713. [Google Scholar] [CrossRef] [Green Version]
  72. Artyukhin, A.B.; Yim, J.J.; Srinivasan, J.; Izrayelit, Y.; Bose, N.; von Reuss, S.H.; Jo, Y.; Jordan, J.M.; Baugh, L.R.; Cheong, M.; et al. Succinylated Octopamine Ascarosides and a New Pathway of Biogenic Amine Metabolism in Caenorhabditis elegans. J. Biol. Chem. 2013, 288, 18778–18783. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. Izrayelit, Y.; Srinivasan, J.; Campbell, S.L.; Jo, Y.; von Reuss, S.H.; Genoff, M.C.; Sternberg, P.W.; Schroeder, F.C. Targeted Metabolomics Reveals a Male Pheromone and Sex-Specific Ascaroside Biosynthesis in Caenorhabditis elegans. ACS Chem. Biol. 2012, 7, 1321–1325. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  74. Choe, A.; Chuman, T.; von Reuss, S.H.; Dossey, A.T.; Yim, J.J.; Ajredini, R.; Kolawa, A.A.; Kaplan, F.; Alborn, H.T.; Teal, P.E.; et al. Sex-Specific Mating Pheromones in the Nematode Panagrellus redivivus. Proc. Natl. Acad. Sci. USA 2012, 109, 20949–20954. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  75. Shi, C.; Runnels, A.M.; Murphy, C.T. Mating and Male Pheromone Kill Caenorhabditis Males Through Distinct Mechanisms. Elife 2017, 6, 23493. [Google Scholar] [CrossRef]
  76. Aprison, E.Z.; Dzitoyeva, S.; Angeles-Albores, D.; Ruvinsky, I. A Male Pheromone that Improves the Quality of the Oogenic Germline. Proc. Natl. Acad. Sci. USA 2022, 119, e2015576119. [Google Scholar] [CrossRef]
  77. Aprison, E.Z.; Ruvinsky, I. Sex Pheromones of C. elegans Males Prime the Female Reproductive System and Ameliorate the Effects of Heat Stress. PLoS Genet. 2015, 11, e1005729. [Google Scholar] [CrossRef] [Green Version]
  78. Aprison, E.Z.; Ruvinsky, I. Sexually Antagonistic Male Signals Manipulate Germline and Soma of C. elegans Hermaphrodites. Curr. Biol. 2016, 26, 2827–2833. [Google Scholar] [CrossRef] [Green Version]
  79. Aprison, E.Z.; Ruvinsky, I. Counteracting Ascarosides Act through Distinct Neurons to Determine the Sexual Identity of C. elegans Pheromones. Curr. Biol. 2017, 27, 2589–2599.e3. [Google Scholar] [CrossRef] [Green Version]
  80. Dong, C.; Dolke, F.; von Reuss, S.H. Selective MS Screening Reveals a Sex Pheromone in Caenorhabditis briggsae and Species-Specificity in Indole Ascaroside Signalling. Org. Biomol. Chem. 2016, 14, 7217–7225. [Google Scholar] [CrossRef] [Green Version]
  81. Balakanich, S.; Samoiloff, M.R. Development of Nematode Behavior: Sex Attraction Among Different Strains of the Free-Living Panagrellus redivivus. Can. J. Zool. 1974, 52, 835–845. [Google Scholar] [CrossRef]
  82. Chaudhuri, J.; Kache, V.; Pires-daSilva, A. Regulation of Sexual Plasticity in a Nematode that Produces Males, Females, and Hermaphrodites. Curr. Biol. 2011, 21, 1548–1551. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  83. Chaudhuri, J.; Bose, N.; Tandonnet, S.; Adams, S.; Zuco, G.; Kache, V.; Parihar, M.; von Reuss, S.H.; Schroeder, F.C.; Pires-daSilva, A. Mating Dynamics in a Nematode with Three Sexes and Its Evolutionary Implications. Sci. Rep. 2015, 5, 17676. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  84. Meyer, S.L.; Johnson, G.; Dimock, M.; Fahey, J.W.; Huettel, R.N. Field Efficacy of Verticillium lecanii, Sex Pheromone, and Pheromone Analogs as Potential Management Agents for Soybean Cyst Nematode. J. Nematol. 1997, 29, 282–288. [Google Scholar] [PubMed]
  85. Aumann, J.; Dietsche, E.; Rutencrantz, S.; Ladehoff, H. Physico-Chemical Properties of the Female Sex Pheromone of Heterodera schachtii (Nematoda: Heteroderidae). Int. J. Parasitol. 1998, 28, 1691–1694. [Google Scholar] [CrossRef]
  86. Shinya, R.; Chen, A.; Sternberg, P.W. Sex Attraction and Mating in Bursaphelenchus okinawaensis and B. xylophilus. J. Nematol. 2015, 47, 176–183. [Google Scholar] [PubMed]
  87. Cepulyte, R.; Bu da, V. Toward Chemical Ecology of Plant-Parasitic Nematodes: Kairomones, Pheromones, and Other Behaviorally Active Chemical Compounds. J. Agric. Food Chem. 2022, 70, 1367–1390. [Google Scholar] [CrossRef] [PubMed]
  88. Riga, E.; Holdsworth, D.R.; Perry, R.N.; Barrett, J.; Johnston, M.R. Electrophysiological Analysis of the Response of Males of the Potato Cyst Nematode, Globodera rostochiensis, to Fractions of Their Homospecific Sex Pheromone. Parasitology 1997, 115 Pt 3, 311–316. [Google Scholar] [CrossRef]
  89. Faghih, N.; Bhar, S.; Zhou, Y.; Dar, A.R.; Mai, K.; Bailey, L.S.; Basso, K.B.; Butcher, R.A. A Large Family of Enzymes Responsible for the Modular Architecture of Nematode Pheromones. J. Am. Chem. Soc. 2020, 142, 13645–13650. [Google Scholar] [CrossRef]
  90. Macosko, E.Z.; Pokala, N.; Feinberg, E.H.; Chalasani, S.H.; Butcher, R.A.; Clardy, J.; Bargmann, C.I. A Hub-and-Spoke Circuit Drives Pheromone Attraction and Social Behaviour in C. elegans. Nature 2009, 458, 1171–1175. [Google Scholar] [CrossRef] [Green Version]
  91. Hussey, R.; Stieglitz, J.; Mesgarzadeh, J.; Locke, T.T.; Zhang, Y.K.; Schroeder, F.C.; Srinivasan, S. Pheromone-Sensing Neurons Regulate Peripheral Lipid Metabolism in Caenorhabditis elegans. PLoS Genet. 2017, 13, e1006806. [Google Scholar] [CrossRef] [Green Version]
  92. Luo, J.; Portman, D.S. Sex-Specific, Pdfr-1-Dependent Modulation of Pheromone Avoidance by Food Abundance Enables Flexibility in C. elegans Foraging Behavior. Curr. Biol. 2021, 31, 4449–4461.e4. [Google Scholar] [CrossRef]
  93. Jang, H.; Kim, K.; Neal, S.J.; Macosko, E.; Kim, D.; Butcher, R.A.; Zeiger, D.M.; Bargmann, C.I.; Sengupta, P. Neuromodulatory State and Sex Specify Alternative Behaviors Through Antagonistic Synaptic Pathways in C. elegans. Neuron 2012, 75, 585–592. [Google Scholar] [CrossRef] [Green Version]
  94. Fagan, K.A.; Luo, J.; Lagoy, R.C.; Schroeder, F.C.; Albrecht, D.R.; Portman, D.S. A Single-Neuron Chemosensory Switch Determines the Valence of a Sexually Dimorphic Sensory Behavior. Curr. Biol. 2018, 28, 902–914.e5. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  95. Zhang, Y.K.; Sanchez-Ayala, M.A.; Sternberg, P.W.; Srinivasan, J.; Schroeder, F.C. Improved Synthesis for Modular Ascarosides Uncovers Biological Activity. Org. Lett. 2017, 19, 2837–2840. [Google Scholar] [CrossRef] [Green Version]
  96. Ben Arous, J.; Laffont, S.; Chatenay, D. Molecular and Sensory Basis of a Food Related Two-State Behavior in C. elegans. PLoS ONE 2009, 4, e7584. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  97. Greene, J.S.; Brown, M.; Dobosiewicz, M.; Ishida, I.G.; Macosko, E.Z.; Zhang, X.; Butcher, R.A.; Cline, D.J.; McGrath, P.T.; Bargmann, C.I. Balancing Selection Shapes Density-Dependent Foraging Behaviour. Nature 2016, 539, 254–258. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  98. Dal Bello, M.; Perez-Escudero, A.; Schroeder, F.C.; Gore, J. Inversion of Pheromone Preference Optimizes Foraging in C. elegans. Elife 2021, 10, 58144. [Google Scholar] [CrossRef]
  99. Nuttley, W.M.; Atkinson-Leadbeater, K.P.; Van Der Kooy, D. Serotonin Mediates Food-Odor Associative Learning in the Nematode Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2002, 99, 12449–12454. [Google Scholar] [CrossRef] [Green Version]
  100. Hong, M.; Ryu, L.; Ow, M.C.; Kim, J.; Je, A.R.; Chinta, S.; Huh, Y.H.; Lee, K.J.; Butcher, R.A.; Choi, H.; et al. Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans. Curr. Biol. 2017, 27, 3168–3177.e3. [Google Scholar] [CrossRef] [Green Version]
  101. Manosalva, P.; Manohar, M.; von Reuss, S.H.; Chen, S.; Koch, A.; Kaplan, F.; Choe, A.; Micikas, R.J.; Wang, X.; Kogel, K.H.; et al. Conserved Nematode Signalling Molecules Elicit Plant Defenses and Pathogen Resistance. Nat. Commun. 2015, 6, 7795. [Google Scholar] [CrossRef] [Green Version]
  102. Kaplan, F.; Perret-Gentil, A.; Giurintano, J.; Stevens, G.; Erdogan, H.; Schiller, K.C.; Mirti, A.; Sampson, E.; Torres, C.; Sun, J.; et al. Conspecific and Heterospecific Pheromones Stimulate Dispersal of Entomopathogenic Nematodes During Quiescence. Sci. Rep. 2020, 10, 5738. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  103. Kaplan, F.; Alborn, H.T.; von Reuss, S.H.; Ajredini, R.; Ali, J.G.; Akyazi, F.; Stelinski, L.L.; Edison, A.S.; Schroeder, F.C.; Teal, P.E. Interspecific Nematode Signals Regulate Dispersal Behavior. PLoS ONE 2012, 7, e38735. [Google Scholar] [CrossRef] [Green Version]
  104. Hartley, C.J.; Lillis, P.E.; Owens, R.A.; Griffin, C.T. Infective Juveniles of Entomopathogenic Nematodes (Steinernema and Heterorhabditis) Secrete Ascarosides and Respond to Interspecific Dispersal Signals. J. Invertebr. Pathol. 2019, 168, 107257. [Google Scholar] [CrossRef] [PubMed]
  105. Wang, J.; Cao, L.; Huang, Z.; Gu, X.; Cui, Y.; Li, J.; Li, Y.; Xu, C.; Han, R. Influence of the Ascarosides on the Recovery, Yield and Dispersal of Entomopathogenic Nematodes. J. Invertebr. Pathol. 2022, 188, 107717. [Google Scholar] [CrossRef]
  106. Kong, X.; Huang, Z.; Gu, X.; Cui, Y.; Li, J.; Han, R.; Jin, Y.; Cao, L. Dimethyl Sulfoxide and Ascarosides Improve the Growth and Yields of Entomopathogenic Nematodes in Liquid Cultures. J. Invertebr. Pathol. 2022, 193, 107800. [Google Scholar] [CrossRef] [PubMed]
  107. Zhao, L.; Zhang, X.; Wei, Y.; Zhou, J.; Zhang, W.; Qin, P.; Chinta, S.; Kong, X.; Liu, Y.; Yu, H.; et al. Ascarosides Coordinate the Dispersal of a Plant-Parasitic Nematode with the Metamorphosis of Its Vector Beetle. Nat. Commun. 2016, 7, 12341. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  108. Zhao, M.; Wickham, J.D.; Zhao, L.; Sun, J. Major Ascaroside Pheromone Component asc-C5 Influences Reproductive Plasticity Among Isolates of the Invasive Species Pinewood Nematode. Integr. Zool. 2021, 16, 893–907. [Google Scholar] [CrossRef] [PubMed]
  109. Meng, J.; Wickham, J.D.; Ren, W.; Zhao, L.; Sun, J. Species Displacement Facilitated by Ascarosides Between Two Sympatric Sibling Species: A Native and Invasive Nematode. J. Pest Sci. 2020, 93, 1059–1071. [Google Scholar] [CrossRef]
  110. Yamada, K.; Hirotsu, T.; Matsuki, M.; Butcher, R.A.; Tomioka, M.; Ishihara, T.; Clardy, J.; Kunitomo, H.; Iino, Y. Olfactory Plasticity is Regulated by Pheromonal Signaling in Caenorhabditis elegans. Science 2010, 329, 1647–1650. [Google Scholar] [CrossRef] [Green Version]
  111. Zhou, Y.; Loeza-Cabrera, M.; Liu, Z.; Aleman-Meza, B.; Nguyen, J.K.; Jung, S.K.; Choi, Y.; Shou, Q.; Butcher, R.A.; Zhong, W. Potential Nematode Alarm Pheromone Induces Acute Avoidance in Caenorhabditis elegans. Genetics 2017, 206, 1469–1478. [Google Scholar] [CrossRef] [Green Version]
  112. Yu, Y.; Zhang, Y.K.; Manohar, M.; Artyukhin, A.B.; Kumari, A.; Tenjo-Castano, F.J.; Nguyen, H.; Routray, P.; Choe, A.; Klessig, D.F.; et al. Nematode Signaling Molecules are Extensively Metabolized by Animals, Plants, and Microorganisms. ACS Chem. Biol. 2021, 16, 1050–1058. [Google Scholar] [CrossRef]
  113. Ning, S.; Zhang, L.; Ma, J.; Chen, L.; Zeng, G.; Yang, C.; Zhou, Y.; Guo, X.; Deng, X. Modular and Scalable Synthesis of Nematode Pheromone Ascarosides: Implications in Eliciting Plant Defense Response. Org. Biomol. Chem. 2020, 18, 4956–4961. [Google Scholar] [CrossRef]
  114. Zhao, L.; Ahmad, F.; Lu, M.; Zhang, W.; Wickham, J.D.; Sun, J. Ascarosides Promote the Prevalence of Ophiostomatoid Fungi and an Invasive Pathogenic Nematode, Bursaphelenchus xylophilus. J. Chem. Ecol. 2018, 44, 701–710. [Google Scholar] [CrossRef]
  115. Yang, C.T.; Vidal-Diez de Ulzurrun, G.; Goncalves, A.P.; Lin, H.C.; Chang, C.W.; Huang, T.Y.; Chen, S.A.; Lai, C.K.; Tsai, I.J.; Schroeder, F.C.; et al. Natural Diversity in the Predatory Behavior Facilitates the Establishment of a Robust Model Strain for Nematode-Trapping Fungi. Proc. Natl. Acad. Sci. USA 2020, 117, 6762–6770. [Google Scholar] [CrossRef]
  116. Hsueh, Y.P.; Mahanti, P.; Schroeder, F.C.; Sternberg, P.W. Nematode-Trapping Fungi Eavesdrop on Nematode Pheromones. Curr. Biol. 2013, 23, 83–86. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  117. Cui, P.; Tian, M.; Huang, J.; Zheng, X.; Guo, Y.; Li, G.; Wang, X. Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora. Pathogens 2022, 11, 997. [Google Scholar] [CrossRef]
  118. Kaplan, F.; Srinivasan, J.; Mahanti, P.; Ajredini, R.; Durak, O.; Nimalendran, R.; Sternberg, P.W.; Teal, P.E.; Schroeder, F.C.; Edison, A.S.; et al. Ascaroside Expression in Caenorhabditis elegans is Strongly Dependent on Diet and Developmental Stage. PLoS ONE 2011, 6, e17804. [Google Scholar] [CrossRef]
  119. Joo, H.J.; Kim, K.Y.; Yim, Y.H.; Jin, Y.X.; Kim, H.; Kim, M.Y.; Paik, Y.K. Contribution of the Peroxisomal Acox Gene to the Dynamic Balance of Daumone Production in Caenorhabditis elegans. J. Biol. Chem. 2010, 285, 29319–29325. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  120. Butcher, R.A. Decoding Chemical Communication in Nematodes. Nat. Prod. Rep. 2017, 34, 472–477. [Google Scholar] [CrossRef] [Green Version]
  121. Hollister, K.A.; Conner, E.S.; Zhang, X.; Spell, M.; Bernard, G.M.; Patel, P.; de Carvalho, A.C.; Butcher, R.A.; Ragains, J.R. Ascaroside. Activity in Caenorhabditis elegans is Highly Dependent on Chemical Structure. Bioorg. Med. Chem. 2013, 21, 5754–5769. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  122. Artyukhin, A.B.; Zhang, Y.K.; Akagi, A.E.; Panda, O.; Sternberg, P.W.; Schroeder, F.C. Metabolomic “Dark Matter” Dependent on Peroxisomal β-Oxidation in Caenorhabditis elegans. J. Am. Chem. Soc. 2018, 140, 2841–2852. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Molecules 28 02409 g001 550
Figure 1. Overview of the chemical structure of development-related pheromones secreted by nematodes. Nacq#1 does not have an ascaroside structure, so it is not shown here, and its detailed structure is shown in Table 1.
Figure 1. Overview of the chemical structure of development-related pheromones secreted by nematodes. Nacq#1 does not have an ascaroside structure, so it is not shown here, and its detailed structure is shown in Table 1.
Molecules 28 02409 g001
Molecules 28 02409 g002 550
Figure 2. Overview of the chemical structures of sex pheromones secreted by nematodes. Vanillic acid does not have an ascaroside structure, so it is not shown here, and its detailed structure is presented in Table 1.
Figure 2. Overview of the chemical structures of sex pheromones secreted by nematodes. Vanillic acid does not have an ascaroside structure, so it is not shown here, and its detailed structure is presented in Table 1.
Molecules 28 02409 g002
Molecules 28 02409 g003 550
Figure 3. Overview of the chemical structure of the aggregation of pheromones secreted by nematodes.
Figure 3. Overview of the chemical structure of the aggregation of pheromones secreted by nematodes.
Molecules 28 02409 g003
Molecules 28 02409 g004 550
Figure 4. Overview of the chemical structures of pheromones with other functions secreted by nematodes.
Figure 4. Overview of the chemical structures of pheromones with other functions secreted by nematodes.
Molecules 28 02409 g004
Molecules 28 02409 g005 550
Figure 5. Pheromones are produced by a wide range of nematode species. The phylogenetic tree was drawn using Mega 4 software based on the comparison of 28S ribosomal RNA (28S) gene sequences obtained from GenBank. The nematodes can be classified into three categories including plant-parasitic nematodes (light green), insect-parasitic nematodes (light grey), and free-living nematodes (light blue). Diverse pheromones can be found widely among nematode groups (red).
Figure 5. Pheromones are produced by a wide range of nematode species. The phylogenetic tree was drawn using Mega 4 software based on the comparison of 28S ribosomal RNA (28S) gene sequences obtained from GenBank. The nematodes can be classified into three categories including plant-parasitic nematodes (light green), insect-parasitic nematodes (light grey), and free-living nematodes (light blue). Diverse pheromones can be found widely among nematode groups (red).
Molecules 28 02409 g005
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Yang, B.; Wang, J.; Zheng, X.; Wang, X. Nematode Pheromones: Structures and Functions. Molecules 2023, 28, 2409. https://doi.org/10.3390/molecules28052409

AMA Style

Yang B, Wang J, Zheng X, Wang X. Nematode Pheromones: Structures and Functions. Molecules. 2023; 28(5):2409. https://doi.org/10.3390/molecules28052409

Chicago/Turabian Style

Yang, Biyuan, Jie Wang, Xi Zheng, and Xin Wang. 2023. "Nematode Pheromones: Structures and Functions" Molecules 28, no. 5: 2409. https://doi.org/10.3390/molecules28052409

Article Metrics

Back to TopTop