Genoprotective Properties and Metabolites of β-Glucan-Rich Edible Mushrooms Following Their In Vitro Fermentation by Human Faecal Microbiota
Abstract
:1. Introduction
2. Results
2.1. Evaluation of Fungal Strains
2.2. Inhibition of Cell Proliferation and Genoprotection
2.2.1. Pilot Study with P. ostreatus Strain 1123
2.2.2. Validation Study
2.3. NMR-Based Metabolomics Profiling
3. Discussion
4. Materials and Methods
4.1. Fungal Strains, Mushroom Cultivation and Determination of Glucans Content
4.2. Faecal Sample Collection and In Vitro Static Batch Culture Fermentations
4.3. Cell Culture and Treatment
4.4. Cell Proliferation Sssays
4.5. Comet Assay
4.6. NMR-Based Metabolomics
4.6.1. Sample Preparation
4.6.2. NMR Spectroscopy
4.6.3. Interpretation of Spectra—Metabolites Assignment
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Not available. |
Paired Differences | ||||||||
---|---|---|---|---|---|---|---|---|
Samples | N | Mean | SD | Interval of the Lower Upper | t | Df | Sig. (2-Tailed) | |
PE | 8 | 10.80 | 11.36 | 1.30 | 20.30 | 2.69 | 7.00 | 0.03 |
PO | 8 | 6.88 | 13.92 | −4.76 | 18.52 | 1.40 | 7.00 | 0.20 |
POL | 8 | 7.66 | 9.16 | 0.01 | 15.32 | 2.37 | 7.00 | 0.05 |
CC2 | 7 | −2.23 | 15.93 | −16.96 | 12.50 | −0.37 | 6.00 | 0.72 |
CC505 | 7 | 4.00 | 12.25 | −7.33 | 15.33 | 0.86 | 6.00 | 0.42 |
INU | 8 | 7.35 | 12.10 | −2.76 | 17.47 | 1.72 | 7.00 | 0.13 |
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Boulaka, A.; Christodoulou, P.; Vlassopoulou, M.; Koutrotsios, G.; Bekiaris, G.; Zervakis, G.I.; Mitsou, E.K.; Saxami, G.; Kyriacou, A.; Zervou, M.; et al. Genoprotective Properties and Metabolites of β-Glucan-Rich Edible Mushrooms Following Their In Vitro Fermentation by Human Faecal Microbiota. Molecules 2020, 25, 3554. https://doi.org/10.3390/molecules25153554
Boulaka A, Christodoulou P, Vlassopoulou M, Koutrotsios G, Bekiaris G, Zervakis GI, Mitsou EK, Saxami G, Kyriacou A, Zervou M, et al. Genoprotective Properties and Metabolites of β-Glucan-Rich Edible Mushrooms Following Their In Vitro Fermentation by Human Faecal Microbiota. Molecules. 2020; 25(15):3554. https://doi.org/10.3390/molecules25153554
Chicago/Turabian StyleBoulaka, Athina, Paris Christodoulou, Marigoula Vlassopoulou, Georgios Koutrotsios, Georgios Bekiaris, Georgios I. Zervakis, Evdokia K. Mitsou, Georgia Saxami, Adamantini Kyriacou, Maria Zervou, and et al. 2020. "Genoprotective Properties and Metabolites of β-Glucan-Rich Edible Mushrooms Following Their In Vitro Fermentation by Human Faecal Microbiota" Molecules 25, no. 15: 3554. https://doi.org/10.3390/molecules25153554