Plant Tissue Culture for Crop Improvement

A special issue of Methods and Protocols (ISSN 2409-9279). This special issue belongs to the section "Molecular and Cellular Biology".

Deadline for manuscript submissions: 20 August 2024 | Viewed by 435

Special Issue Editor

E-Mail Website
Guest Editor
Aquatic and Crop Resource Development Centre, National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK S7N 0W9, Canada
Interests: plant tissue culture; transformation; gene editing; microscopic and analytical characterization and image-based phenotyping; trait improvement; accelerated breeding

Special Issue Information

Dear Colleagues,

Plant tissue culture and genome editing are expanding traditional plant breeding tools for crop improvement by introducing new traits more quickly and precisely. Our main objective is to advance the knowledge on plant tissue culture and genome editing tools which can be deployed efficiently to multiple crop species. This Special Issue will focus on the recent advancement in the techniques of gene editing using protoplast, cell, tissue and organ culture, and the regeneration of whole plants for improving different traits in cereals, oilseeds, pulse crops and greenhouse vegetables and fruits. Authors are invited to submit contributions in all areas related to the optimization of the promoters to drive and express Cas9 and the utilization of different fluorescent reporters and selection markers; delivery methods including electroporation, biolistic and Agrobacterium-mediated transformation and plant regeneration for achieving high-efficiency genome editing. These may include the optimization of mesophyll protoplasts system for gRNA validation and the delivery of gene editing components into haploid immature pollen. We welcome original research papers and critical reviews of recent developments in accelerated-breeding-related disciplines (prior consultation with the Guest Editor is recommended). Short research reports or case studies describing a novel DNA or protein delivery systems are also welcome.

Dr. Pankaj Bhowmik
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • plant protoplasts
  • microspore transformation
  • guide RNA validation
  • genome editing
  • DNA delivery
  • biolistic delivery
  • grafting and plant regeneration

Published Papers

This special issue is now open for submission.
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