Down the Drain: A Systematic Review of Molecular Biology Evidence Linking Sinks with Bacterial Healthcare-Associated Infections in Intensive Care Units
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Identification and Selection of Studies
3.2. Main Characteristics of the Selected Studies
Reference | Setting | Type of Study | Duration (Outbreak and/ or Study) | Molecular Biology Technique(s) | Species/Group of Species Investigated |
---|---|---|---|---|---|
McGeer et al. [11] | NICU 1 | Prospective | 8 months | REA 1 | Serratia marcescens |
Orr et al. [22] | ICU 1 | Retrospective | 19 days | Pyrolysis Mass Spectrometry | Stenotrophomonas maltophilia |
Döring et al. [13] | ICU 1 | Prospective | 3 months | Exotoxin A DNA probe | Pseudomonas aeruginosa |
Kerr at al. [23] | ICU 1 | Retrospective | 3 months | RAPD 1 | Pseudomonas aeruginosa |
Verweij et al. [24] | NICU 1 | Retrospective | 3 months | RAPD 1 | Stenotrophomonas maltophilia |
Su et al. [25] | PICU 1 | Retrospective | Not provided | Infrequent restriction site PCR 1 PFGE 1 | Klebsiella pneumoniae |
Hoque et al. [26] | NICU 1 | Retrospective | 21 months | PFGE 1 | Elizabethkingia meningoseptica |
van Dijk et al. [27] | NICU 1 | Retrospective | 11 months | AFLP 1 Rep-PCR 1 | Enterobacter cloacae |
Wagenlehner et al. [18] | ICU 1 | Retrospective | 19 months | PFGE 1 | Enterobacter spp. |
Milisavljevic et al. [17] | NICUs 1 | Retrospective | 2 weeks (NICU 1 A) 1 month (NICU 1 B) | PFGE 1 | Serratia marcescens |
Kac et al. [28] | ICU 1 | Retrospective | 16 months | RAPD 1 | ESBL 1-producing enterobacteria |
Maragakis et al. [29] | NICU 1 | Retrospective | 5 months | PFGE 1 | Serratia marcescens |
Hota et al. [30] | ICU 1 | Retrospective | 28 months | PFGE 1 | Pseudomonas aeruginosa |
LaForgia et al. [31] | ICU 1 | Retrospective | 10 months | REA 1 | Acinetobacter baumannii |
Longtin et al. [21] | PICU 1 | Retrospective | 30 months | PFGE 1 | Pseudomonas aeruginosa |
Lucero et al. [32] | NICU 1 | Retrospective | 12 months | Rep-PCR 1 | Burkholderia cepacia complex |
Guleri et al. [33] | ICU 1 | Retrospective | 5 months | Variable Number Tandem Repeat | Pseudomonas aeruginosa |
Diederen et al. [34] | ICU 1 | Retrospective | 61 months | Unspecified molecular biology typing | Pseudomonas aeruginosa |
Maltezou et al. [35] | NICU 1 | Retrospective | 34 months | PFGE 1 | Serratia marcescens |
Lowe et al. [36] | ICU 1 | Retrospective | 54 months | PFGE 1 | Klebsiella oxytoca |
Starlander et al. [37] | ICU 1 | Retrospective | 7 months | PFGE 1 | Klebsiella pneumoniae |
Kotsanas et al. [38] | ICU 1 | Retrospective | 33 months | PFGE 1 | CPE 1 |
Landelle et al. [39] | ICU 1 | Restrospective | 17 months | PFGE 1 | Acinetobacter baumannii |
Vergara-Lopez et al. [40] | ICU 1 | Retrospective | 33 months | PFGE 1 | Klebsiella oxytoca |
Wendel et al. [19] | ICU 1 | Retro- & Prospective | 11 years | MLST 1 | Pseudomonas aeruginosa |
Davis et al. [41] | PICU 1 | Retrospective | 4 months | Rep-PCR 1 WGS 1 | Pseudomonas aeruginosa |
Umezawa et al. [42] | ICU 1 | Retrospective | 1 month | Rep-PCR 1 MLST 1 | Acinetobacter baumannii |
Zhou et al. [14] | ICU 1 | Prospective | 8 months | PFGE 1 | Pseudomonas aeruginosa |
Salm et al. [43] | ICU 1 | Retrospective | 28 months | Rep-PCR | Pseudomonas aeruginosa |
De Geyter et al. [44] | ICU 1 | Retrospective | 8 months | PFGE 1 | CPE 1 |
Herruzo et al. [45] | NICU 1 | Retrospective | 5 months | Rep-PCR 1 | Klebsiella oxytoca |
Cantero et al. [46] | ICU 1 | Retrospective | 6 weeks | PFGE 1 | Chryseobacterium indologenes |
Regev-Yochay et al. [47] | ICU 1 | Retrospective | 17 months | PFGE 1 | Serratia marcescens |
Martischang et al. [48] | ICU 1 | Retrospective | 2 consecutive summers | WGS 1 | Serratia marcescens |
Eveillard et al. [9] | ICU 1 | Retro- & Prospective | 5 years | PFGE 1 | Klebsiella pneumoniae |
Avaness et al. [49] | ICU 1 | Retrospective | 3 months | Plasmid analysis | CPE 1 |
Jung et al. [50] | ICU 1 | Retrospective | 6 months | PFGE 1 | CPE 1 |
Yeo et al. [51] | NICU 1 | Retrospective | 4 months | Rep-PCR 1 WGS 1 | Serratia marcescens |
de Man et al. [52] | ICU 1 | Retrospective | 10 months | WGS 1 | CPE 1 |
Qiao et al. [15] | ICU 1 & NICU 1 | Prospective | 6 months | WGS 1 | Klebsiella oxytoca & Klebsiella pneumoniae |
Lemarié et al. [16] | ICU 1 | Prospective | 12 months | PFGE 1 | CPE 1 |
Gideskog et al. [53] | ICU 1 | Retrospective | 1 month | WGS 1 | Stenotrophomonas maltophilia |
Blanc et al. [20] | NICU 1 | Retro- & Prospective | 4 months | WGS 1 | Pseudomonas aeruginosa |
Catho et al. [54] | ICU 1 | Retrospective | 31 months | WGS 1 | Pseudomonas aeruginosa |
Bourdin et al. [55] | NICU 1 | Retrospective | Not provided | HiSST 1 | Serratia marcescens |
3.3. Risk of Bias and Quality Assessment of the Selected Studies
3.4. Bacterial Species Involved
3.5. Molecular Typing Methods Used for Attribution of Bacterial Isolates to Clones
3.5.1. Random Amplified Polymorphic DNA (RAPD)
3.5.2. Restriction Endonuclease Analysis (REA) and Pulse Field Gel Electrophoresis (PFGE)
3.5.3. Repetitive Element Palindromic-PCR (Rep-PCR)
3.5.4. MultiLocus Sequence Typing (MLST)
3.5.5. Whole Genome Sequencing (WGS) and In Silico MLST
3.5.6. Miscellaneous Techniques
3.6. Link between Sink and Patients Isolates
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Reference | Study Design 1 | Precise Clinical and Environmental Positivity Timeline 2 | Molecular Biology Technique Cut-Off Threshold Provided 2 | Molecular Biology Analysis of All Isolates Reported 2 | External Control Strain(s) 2 | Overall Score 3 |
---|---|---|---|---|---|---|
McGeer et al. [11] | Prospective | 1 | 0 | 0 | 0 | 4 |
Orr et al. [22] | Retrospective cohort | 0 | 1 | 0 | 1 | 3 |
Döring et al. [13] | Prospective | 1 | 0 | 0 | 0 | 4 |
Kerr at al. [23] | Retrospective cohort | 0 | 1 | 1 | 1 | 4 |
Verweij et al. [24] | Retrospective cohort | 1 | 1 | 1 | 1 | 5 |
Su et al. [25] | Retrospective cohort | 0 | 1 | 1 | 1 | 4 |
Hoque et al. [26] | Retrospective cohort | 1 | 0 | 0 | 0 | 2 |
van Dijk et al. [27] | Retrospective cohort | 0 | 0 | 0 | 1 | 2 |
Wagenlehner et al. [18] | Retrospective cohort | 0 | 1 | 0 | 1 | 3 |
Milisavljevic et al. [17] | Retrospective cohort | 0 | 1 | 1 | 0 | 3 |
Kac et al. [28] | Retrospective cohort | 0 | 1 | 1 | 0 | 3 |
Maragakis et al. [29] | Retrospective cohort | 0 | 1 | 0 | 0 | 2 |
Hota et al. [30] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
LaForgia et al. [31] | Retrospective cohort | 1 | 1 | 0 | 0 | 3 |
Longtin et al. [21] | Retrospective cohort | 0 | 1 | 0 | 0 | 2 |
Lucero et al. [32] | Case-control | 0 | 1 | 0 | 0 | 3 |
Guleri et al. [33] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
Diederen et al. [34] | Case-control | 0 | 0 | 0 | 0 | 2 |
Maltezou et al. [35] | Case-control | 0 | 1 | 1 | 0 | 4 |
Lowe et al. [36] | Retrospective cohort | 0 | 0 | 1 | 0 | 2 |
Starlander et al. [37] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
Kotsanas et al. [38] | Retrospective cohort | 0 | 1 | 0 | 0 | 2 |
Landelle et al. [39] | Retrospective cohort | 1 | 0 | 0 | 0 | 2 |
Vergara-Lopez et al. [40] | Retrospective cohort | 1 | 0 | 0 | 0 | 2 |
Wendel et al. [19] | Retrospective cohort 4 | 1 | 1 | 0 | 0 | 3 |
Davis et al. [41] | Retrospective cohort | 1 | 1 | 0 | 0 | 3 |
Umezawa et al. [42] | Retrospective cohort | 0 | 1 | 1 | 0 | 3 |
Zhou et al. [14] | Prospective | 1 | 1 | 1 | 0 | 6 |
Salm et al. [43] | Case-control | 0 | 1 | 1 | 0 | 4 |
De Geyter et al. [44] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
Herruzo et al. [45] | Retrospective cohort | 1 | 0 | 1 | 0 | 3 |
Cantero et al. [46] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
Regev-Yochay et al. [47] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
Martischang et al. [48] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
Eveillard et al. [9] | Retrospective cohort 4 | 0 | 1 | 0 | 0 | 2 |
Avaness et al. [49] | Retrospective cohort | 0 | 0 | 0 | 0 | 1 |
Jung et al. [50] | Case-Control | 1 | 0 | 0 | 0 | 3 |
Yeo et al. [51] | Retrospective cohort | 1 | 1 | 1 | 1 | 5 |
de Man et al. [52] | Retrospective cohort | 0 | 0 | 0 | 1 | 2 |
Qiao et al. [15] | Prospective | 1 | 1 | 1 | 0 | 6 |
Lemarié et al. [16] | Prospective | 0 | 1 | 1 | 0 | 5 |
Gideskog et al. [53] | Retrospective cohort | 0 | 1 | 1 | 0 | 3 |
Blanc et al. [20] | Retrospective cohort 4 | 0 | 1 | 1 | 0 | 3 |
Catho et al. [54] | Retrospective cohort | 1 | 1 | 0 | 1 | 4 |
Bourdin et al. [55] | Retrospective cohort | 0 | 1 | 0 | 1 | 3 |
Overall reporting frequency | 33.3% (15/45) | 57.8% (26/45) | 35.6% (16/45) | 22.2% (10/45) |
Group/Species | Antibiotic Resistance Profile | Possible Link 1,2 | No Proven Link 2 |
---|---|---|---|
Non-fermentative Gram-negative bacteria | |||
Acinetobacter baumannii | MDR 3 | REA 3 [31] | - |
PFGE 3 [39] | - | ||
AM, CIP 3 | MLST 3 & Rep-PCR 3 [42] | - | |
Burkholderia cepacia complex | Not reported | Rep-PCR [32] | - |
Chryseobacterium indologenes | Not reported | PFGE [46] | - |
Elizabethkingia meningoseptica | MDR | PFGE [26] | - |
Pseudomonas aeruginosa | Not reported Intrinsic resistance only | Exotoxine A DNA probe [13] PFGE [14] RAPD 3 [23] PFGE [21] Rep-PCR & WGS 3 [41] | - WGS [20] |
GIM 3-1 carbapenemase | MLST [19] | - | |
MDR | PFGE [30] Rep-PCR [43] | Variable number tandem-repeat [33] | |
VIM 3 carbapenemase | WGS [54] | Unspecified molecular typing method [34] | |
Stenotrophomonas maltophilia | Susceptible to TMP-SFX 3 | WGS [53] | WGS [53] |
Not reported | RAPD [24] | Pyrolysis mass spectrometry [22] | |
Enterobacteria | OXA 3-48 carbapenemase | - | PFGE [16] |
VIM carbapenemase | - | WGS [52] | |
Carbapenemase-producing | PFGE [50] | PFGE [38,44] Plasmid analysis [49] | |
ESBL 3 | RAPD [28] | - | |
Citrobacter freundii | OXA-48 carbapenemase | PFGE [44] | - |
Enterobacter spp. | Variable | PFGE [18] | - |
Enterobacter cloacae | ESBL | - | Rep-PCR [27] |
Klebsiella oxytoca | VIM carbapenemase | - | Rep-PCR [45] |
IMP 3-8 carbapenemase | PFGE [40] | - | |
Carbapenem-resistant | WGS [15] | - | |
ESBL | PFGE [36] | - | |
Klebsiella pneumoniae | OXA-48 carbapenemase | PFGE [9] | - |
Carbepenem-resistant | WGS [15] | - | |
ESBL | PFGE [37] | - | |
Variable | PFGE [25] | ||
Serratia marcescens | Not provided | HiSST [55] | Rep-PCR and WGS [51] |
Intrinsic resistance only | PFGE [35] | PFGE [29] | |
MDR | - | PFGE [17] | |
Variable | REA [11] | WGS [48] | |
OXA-48 carbapenemase | PFGE [47] | - |
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Choquet, M.; Mullié, C. Down the Drain: A Systematic Review of Molecular Biology Evidence Linking Sinks with Bacterial Healthcare-Associated Infections in Intensive Care Units. Hygiene 2022, 2, 94-108. https://doi.org/10.3390/hygiene2020008
Choquet M, Mullié C. Down the Drain: A Systematic Review of Molecular Biology Evidence Linking Sinks with Bacterial Healthcare-Associated Infections in Intensive Care Units. Hygiene. 2022; 2(2):94-108. https://doi.org/10.3390/hygiene2020008
Chicago/Turabian StyleChoquet, Morgane, and Catherine Mullié. 2022. "Down the Drain: A Systematic Review of Molecular Biology Evidence Linking Sinks with Bacterial Healthcare-Associated Infections in Intensive Care Units" Hygiene 2, no. 2: 94-108. https://doi.org/10.3390/hygiene2020008