Antifouling Studies of Unsymmetrical Oligo(ethylene glycol) Spiroalkanedithiol Self-Assembled Monolayers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Substrates
2.3. Preparation of SAMs
2.4. Protein Preparation/Exposure
2.5. SPR Procedures
2.6. QCM Measurements
2.7. Ellipsometry Measurements
3. Results and Discussion
3.1. In Situ Analysis of Protein Adsorption Using SPR Spectroscopy
3.2. Ex Situ Analysis of Protein Adsorption Using QCM
3.3. Ellipsometric Thickness Measurements
3.4. Wettability of the SAMs
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Protein | Protamine | Lysozyme | BSA | Fibrinogen |
---|---|---|---|---|
Molecular weight | 4 KDa | 14 KDa | 55 KDa | 340 KDa |
Size | 5 Å c | 18 Å c | 140 × 40 × 40 Å | 450 × 90 Å |
Shape | Spherical | Stubby prolate ellipsoid | Prolate ellipsoid a | Cylindrical b |
pI | 12.1 | 11.1 | 4.8 | 5.7 |
Application | Insulin | Cell | Blood | Muscle/Tissue |
SAM | ∆ RU | |||
---|---|---|---|---|
Protamine | Lysozyme | BSA | Fibrinogen | |
C18SH | 599 ± 3 | 1029 ± 42 | 899 ± 63 | 2285 ± 222 |
EG3C7SH | 173 ± 11 | 108 ± 20 | 195 ± 53 | 201 ± 46 |
EG3C7-C7 | 368 ± 87 | 1163 ± 77 | 1121 ± 145 | 2194 ± 122 |
EG3C7-C18 | 936 ± 48 | 1524 ± 80 | 1051 ± 96 | 2295 ± 24 |
SAM | Protein Loading (ng/cm2) | |||
---|---|---|---|---|
Protamine | Lysozyme | BSA | Fibrinogen | |
C18SH | 329 ± 34 | 409 ± 67 | 493 ± 76 | 854 ± 80 |
EG3C7SH | 218 ± 94 | 264 ± 12 | 223 ± 75 | 119 ± 30 |
EG3C7-C7 | 308 ± 40 | 314 ± 62 | 621 ± 72 | 471 ± 52 |
EG3C7-C18 | 398 ± 45 | 442 ± 76 | 644 ± 99 | 943 ± 83 |
SAM | ∆ Thickness (Å) | |||
---|---|---|---|---|
Protamine | Lysozyme | BSA | Fibrinogen | |
C18SH | 11 ± 5 | 20 ± 2 | 13 ± 8 | 43 ± 2 |
EG3C7SH | 2 ± 2 | 2 ± 1 | 2 ± 3 | 3 ± 1 |
EG3C7-C7 | 3 ± 3 | 19 ± 2 | 8 ± 2 | 27 ± 1 |
EG3C7-C18 | 9 ± 4 | 20 ± 7 | 16 ± 4 | 39 ± 2 |
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St. Hill, L.R.; Tran, H.-V.; Chinwangso, P.; Lee, H.J.; Marquez, M.D.; Craft, J.W., Jr.; Lee, T.R. Antifouling Studies of Unsymmetrical Oligo(ethylene glycol) Spiroalkanedithiol Self-Assembled Monolayers. Micro 2021, 1, 151-163. https://doi.org/10.3390/micro1010012
St. Hill LR, Tran H-V, Chinwangso P, Lee HJ, Marquez MD, Craft JW Jr., Lee TR. Antifouling Studies of Unsymmetrical Oligo(ethylene glycol) Spiroalkanedithiol Self-Assembled Monolayers. Micro. 2021; 1(1):151-163. https://doi.org/10.3390/micro1010012
Chicago/Turabian StyleSt. Hill, Lydia R., Hung-Vu Tran, Pawilai Chinwangso, Han Ju Lee, Maria D. Marquez, John W. Craft, Jr., and T. Randall Lee. 2021. "Antifouling Studies of Unsymmetrical Oligo(ethylene glycol) Spiroalkanedithiol Self-Assembled Monolayers" Micro 1, no. 1: 151-163. https://doi.org/10.3390/micro1010012