Response of Senegalese Sorghum Seedlings to Pathotype 5 of Sporisorium reilianum
Abstract
:1. Introduction
2. Methods
2.1. Sorghum Lines
2.2. Seedling Inoculation
2.3. RNA Extraction and Real-Time qRT-PCR Analysis
2.4. Statistical Analysis
3. Results
3.1. Phenotypic Variation for Seedling Inoculation
3.2. Seedling Inoculation Activates Chitinase and PR10
3.3. Seedling Inoculation Is Not as Effective as Syringe Inoculation for Full Disease Development
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Accession | No. of Inoculated Seedlings | No. of Trials | Disease Incidence | Average Time (Days) of Detection | |
---|---|---|---|---|---|
Susceptible (%) | Resistant (%) | ||||
PI514279 * | 13 | 3 | 100 | 0 | 5.8 ± 0.1 a |
PI514284 | 16 | 4 | 100 | 0 | 4.3 ± 0.1 efghijk |
PI514287 | 14 | 3 | 100 | 0 | 4.4 ± 0.3 efghij |
PI514289 | 20 | 4 | 100 | 0 | 3.6 ± 0.1 lm |
PI514295 | 14 | 3 | 100 | 0 | 4.5 ± 0.2 efghij |
PI514306 | 12 | 3 | 100 | 0 | 4.3 ± 0.3 efghijk |
PI514308 | 11 | 3 | 100 | 0 | 5.4 ± 0.2 abc |
PI514309 | 11 | 3 | 100 | 0 | 3.9 ± 0.2 hijklm |
PI514311 | 20 | 4 | 100 | 0 | 3.4 ± 0.1 m |
PI514294 | 23 | 5 | 95.7 | 4.3 | 4.4 ± 0.2 efghij |
PI514316 * | 20 | 4 | 95 | 5 | 4.3 ± 0.2 efghijk |
PI514283 | 17 | 4 | 94.1 | 5.9 | 4.4 ± 0.3 efghijk |
PI514301 | 14 | 3 | 92.9 | 7.1 | 4.5 ± 0.2 efghij |
PI514299 | 23 | 5 | 91.3 | 8.7 | 4.0 ± 0.2 ijkl |
PI514313 | 22 | 5 | 90.9 | 9.1 | 4.0 ± 0.2 hijkl |
PI514297 | 10 | 3 | 90 | 10 | 4.9 ± 0.3 bcde |
PI514298 | 29 | 6 | 89.7 | 10.3 | 5.2 ± 0.2 bcd |
PI514314 | 16 | 3 | 87.5 | 12.5 | 4.6 ± 0.2 defg |
PI514302 | 15 | 3 | 86.7 | 13.3 | 4.7 ± 0.1 cdefg |
PI514312 | 23 | 5 | 82.6 | 17.4 | 3.6 ± 0.2 lm |
PI514282 | 11 | 3 | 81.8 | 18.7 | 4.2 ± 0.3 efghijkl |
PI514285 * | 11 | 3 | 81.8 | 18.7 | 4.6 ± 0.2 defghij |
PI514286 | 11 | 3 | 81.8 | 18.7 | 3.7 ± 0.4 klm |
PI514288 | 11 | 3 | 81.8 | 18.7 | 4.9 ± 0.2 bcde |
PI514290 | 11 | 3 | 81.8 | 18.7 | 3.8 ± 0.3 jklm |
PI514300 * | 10 | 3 | 80 | 20 | 4.6 ± 0.4 cdefghi |
PI514303 | 10 | 3 | 80 | 20 | 4.8 ± 0.3 bcdefg |
PI514310 | 14 | 3 | 78.6 | 21.4 | 4.1 ± 0.3 ghijkl |
PI514291 | 32 | 6 | 71.9 | 28.1 | 4.2 ± 0.2 ghijk |
PI514304 | 24 | 5 | 70.8 | 29.2 | 4.8 ± 0.3 bcdef |
PI514292 | 12 | 3 | 58.3 | 41.7 | 4.0 ± 0.5 fghijklm |
PI514307 | 12 | 3 | 58.3 | 41.7 | 4.0 ± 0.2 fghijklm |
PI514305 | 18 | 4 | 55.6 | 44.4 | 3.7 ± 0.5 klm |
PI514280 | 11 | 3 | 54.5 | 45.5 | 4.3 ± 0.3 efghijkl |
PI514293 | 14 | 3 | 50 | 50 | 4.7 ± 0.4 bcdefgh |
PI514296 | 28 | 5 | 28.6 | 71.4 | 5.5 ± 0.2 ab |
SH1136 (JG) | 10 | 3 | 0 | 100 | - |
SH1152 (JG) | 10 | 3 | 0 | 100 | - |
PI514279 Control | 10 | 3 | 0 | 100 | - |
PI514282 Control | 10 | 3 | 0 | 100 | - |
PI514284 Control | 10 | 3 | 0 | 100 | - |
PI514303 Control | 10 | 3 | 0 | 100 | - |
BTx635 | 10 | 3 | 0 | 100 | - |
BTx643 | 10 | 3 | 10 | 90 | 4 bcdefghijklm |
Accession | Susceptibility (%) | Time (Days) | Fold Change for Chitinase | p-Value Between Two Cultivars | Fold Change for PR10 | p-Value between Two Cultivars |
---|---|---|---|---|---|---|
PI514296 | 28.6 | 5.5 ± 0.2 | 0.8 ± 0.4 | 0.15 | 1.7 ± 0.4 | 0.09 |
PI514311 | 100 | 3.4 ± 0.1 | 2.1 ± 0.7 | 3.9 ± 1.0 |
Accession | Seedling Inoculation (Susceptible/Resistant) | Hypodermic (Syringe) Inoculation (Susceptible/Resistant) | ||
---|---|---|---|---|
PI514279 | R | 0% | R | 0% |
PI514280 | R | 0% | S | 57.1% |
PI514282 | R | 0% | S | 14.3% |
PI514283 | R | 0% | S | 20% |
PI514284 | R | 0% | S | 11.1% |
PI514285 | R | 0% | R | 0% |
PI514286 | R | 0% | S | 22.2% |
PI514287 | R | 0% | S | 8.3% |
PI514288 | R | 0% | S | 75% |
PI514289 | R | 0% | S | 10% |
PI514290 | R | 0% | S | 40% |
PI514291 | R | 0% | S | 33.3% |
PI514292 | R | 0% | S | 33.3% |
PI514293 | R | 0% | S | 11.1% |
PI514294 | R | 0% | S | 45.5% |
PI514295 | R | 0% | S | 27.3% |
PI514296 | R | 0% | S | 22.2% |
PI514297 | R | 0% | S | 50% |
PI514298 | R | 0% | S | 28.6% |
PI514299 | R | 0% | S | 25% |
PI514300 | R | 0% | S | 25% |
PI514301 | R | 0% | S | 70% |
PI514302 | R | 0% | S | 16.5% |
PI514303 | R | 0% | S | 58% |
PI514304 | R | 0% | S | 20% |
PI514305 | R | 0% | S | 30% |
PI514306 | R | 0% | S | 20% |
PI514307 | R | 0% | S | 14.3% |
PI514308 | R | 0% | R | 0% |
PI514309 | R | 0% | S | 33.3% |
PI514310 | R | 0% | S | 16.7% |
PI514311 | R | 0% | S | 55.6% |
PI514312 | R | 0% | S | 80% |
PI514313 | R | 0% | S | 66.7% |
PI514314 | R | 0% | S | 11.1% |
PI514316 | R | 0% | R | 0% |
SH1136 (JG) | R | 0% | - * | - * |
SH1152 (JG) | R | 0% | - * | - * |
PI514279 Control | R | 0% | - | - |
PI514282 Control | R | 0% | - | - |
PI514284 Control | R | 0% | - | - |
PI514303 Control | R | 0% | - | - |
BTx635 (−) | R | 0% | R | 0% |
BTx643 (+) | R | 0% | S | 66.7% |
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Ahn, E.; Prom, L.K.; Fall, C.; Magill, C. Response of Senegalese Sorghum Seedlings to Pathotype 5 of Sporisorium reilianum. Crops 2022, 2, 142-153. https://doi.org/10.3390/crops2020011
Ahn E, Prom LK, Fall C, Magill C. Response of Senegalese Sorghum Seedlings to Pathotype 5 of Sporisorium reilianum. Crops. 2022; 2(2):142-153. https://doi.org/10.3390/crops2020011
Chicago/Turabian StyleAhn, Ezekiel, Louis K. Prom, Coumba Fall, and Clint Magill. 2022. "Response of Senegalese Sorghum Seedlings to Pathotype 5 of Sporisorium reilianum" Crops 2, no. 2: 142-153. https://doi.org/10.3390/crops2020011