Antioxidant Determining Using Electrochemical Method
, Mohamad Rafi 1,2 and Dita Ariyanti 4
Round 1
Reviewer 1 Report
The authors show in this manuscript a very complete review about contemporary developments in electrochemical biosensor techniques and antioxidant evaluation methodologies. They discuss and review the electrochemical method related to sensors, biosensors, and nanosensors.
I consider this manuscript very interesting and useful for the scientific community.
Author Response
Response to Reviewer 1 Comments
Point 1: The authors show in this manuscript a very complete review about contemporary developments in electrochemical biosensor techniques and antioxidant evaluation methodologies. They discuss and review the electrochemical method related to sensors, biosensors, and nanosensors.
I consider this manuscript very interesting and useful for the scientific community.
Response 1: Thank you for the support
Author Response File: 
Author Response.pdf
Reviewer 2 Report
This contribution is a comprehensive, wideranging review, which will be of significant value to workers in the field of the determination of antioxidants. Conventional electrochemical techniques are described, together with the use of biosensors using electrochemistry as the analytical finish.
Written in an easy to follow, scientiific format
Author Response
Response to Reviewer 2 Comments
Point 1: This contribution is a comprehensive, wideranging review, which will be of significant value to workers in the field of the determination of antioxidants. Conventional electrochemical techniques are described, together with the use of biosensors using electrochemistry as the analytical finish.
Response 1: Thank you for the support.
Point 2: Written in an easy to follow, scientiific format
Response 2: Thank you for the suggestion. We have sent our manuscript to colleagues who are fluent in English to improve the English
Author Response File: Author Response.pdf
Reviewer 3 Report
Recommendation: May eventually be publishable, but requires minor revisions as indicated.
Comments:
In this manuscript, Rani Melati Sukma et al. comprehensively analyzed the latest advances in electrochemical biosensing technologies and antioxidant evaluation methods. Overall, some concerns need to be addressed before further consideration. Some comments are listed as follows:
1. Please include more representative pictures in the article.
2. As far as I know, enzyme-based biosensor includes oxidase-based biosensors, please supplement them in 4.1.
3. Please check the reference format carefully.
Minor editing of English language required
Author Response
Response to Reviewer 3 Comments
Recommendation: May eventually be publishable, but requires minor revisions as indicated.
In this manuscript, Rani Melati Sukma et al. comprehensively analyzed the latest advances in electrochemical biosensing technologies and antioxidant evaluation methods. Overall, some concerns need to be addressed before further consideration. Some comments are listed as follows:
Point 1: Please include more representative pictures in the article.
Response 1: Thank you for the suggestion, but we have improved the resolution of the images in this review article
Point 2: As far as I know, enzyme-based biosensor includes oxidase-based biosensors, please supplement them in 4.1.
Response 2: Thank you for the suggestion, we heve supplement oxidase-based biosensors in 4.1 :
A biosensor utilizing oxidase enzymes is capable of effectively neutralizing hydrogen peroxide (H2O2) or superoxide radicals (O2•-) produced through oxidative catalysis. The assessment of antioxidant capacity was carried out by Becker et al. [94] through an examination of multiple fruits using a xanthine oxidase-based biosensor and amperometric techniques. The results revealed distinct antioxidant capacities for each fruit, with the Murici fruit exhibiting the highest capacity. Additionally, the study measured the concentration of gallic acid, finding a positive correlation between higher gallic acid concentrations and increased antioxidant capacity. Furthermore, the assessment of antioxidant capacity was conducted by employing identical enzymes through the utilization of polymeric PVA-AWE films that were immobilized on the carbon paste electrode surface. The chamomile sample exhibited the maximum antioxidant capacity, which was approximately 70% [95]. The present study focuses on the utilization of an ascorbate oxidase-based sensor-biosensor system (SB) for the purpose of detecting ascorbic acid as well as several types of orange juice, specifically Hamlin, Sanguinello, and Moro. The detection method employed in this study utilizes cyclic voltammetry and amperometric methods. The obtained results pertaining to the antioxidant capacity of this particular method were compared to those of the conventional methods, namely DPPH and ABTS. It was observed that there was no significant difference between the antioxidant capacities obtained from the two methods. Notably, the Hamlin variety exhibited the maximum antioxidant capacity [96].
Point 3: Please check the reference format carefully.
Response 3: Thank you for the suggestion, we have improved the reference format
Author Response File: Author Response.pdf
