Impact of Pyrolysis Oil Addition to Ethanol on Combustion in the Internal Combustion Spark Ignition Engine
Round 1
Reviewer 1 Report
Dear authors,
Very briefly, you can find below, some of my specific comments on your manuscript:
10– 13 In the abstract of the paper you said that pyrolysis oil is extremely harmful substance, and it must be neutralized. So, if it is harmful, it should not be used as fuel, and its neutralization is not mentioned in the text. So, I would like a deeper argumentation of the advantages of using this fuel / additive.
45-64 You have talked about the physico-chemical properties of pyrolysis oil and made comparison with diesel and gasoline. When talking about viscosity and density, in order to evaluate these properties, it is necessary to specify the temperature at which they were measured.
69-99 you have talked about the use of pyrolysis oil in diesel engines, then, (100-122) you have talked about its use in spark ignition engines, then, again about diesel ... I think the bibliographic study should be structured, on the two types of engines and presented separate arguments and studies.
125-126 I think it should be specified exactly what engine performance you intend to study in this paper.
137 Table 2 containing the properties of pyrolysis oil should be redone. I think a comparison of the properties for the three substances should be made: ethanol, pyrolysis oil and mixture.
I think it is important to specify how you measured the physical properties and chemical composition of the pyrolysis oil, what devices and what methods you used.
139 Table 3 - I think needs to be rewritten. It is hard to read. The tests should be described in more detail.
181, 217 there are two tables 5.
214, 228 fig 2, 3 - I don't understand why the trend of parameter variation has to be traced. Explain, please!
232…. paragraph 3.2. I think the mathematical model should be presented. The authors made only a story; the equations that were used should be presented.
309-313 Finally, why was the 3: 1 oil-ethanol mixture used? Why is this report recommended? I believe that a comparative analysis of several mixing percentages should be made.
Taking into consideration the above, I propose to accept the manuscript with the major changes.
Author Response
Very briefly, you can find below, some of my specific comments on your manuscript:
10– 13 In the abstract of the paper you said that pyrolysis oil is extremely harmful substance, and it must be neutralized. So, if it is harmful, it should not be used as fuel, and its neutralization is not mentioned in the text. So, I would like a deeper argumentation of the advantages of using this fuel / additive.
Fuels for IC engines usually are hydrocarbons (CxHy) and oxygenated hydrocarbons CxHyOz. All of them are considered as harmful for living creatures. Pyrolysis oil is just a mixture of various hydrocarbons. At combustion it is oxidized to water and CO2. There might be some trace concentrations of sulphur that is oxidized to SOx. Hence, combustion products, except SOx, cannot be managed as harmful for both natural environment and humans and combustion can be treated as the best neutralization technology in this case. With respect to your remark, you are right. We added to the statement that pyrolysis oil is particularly harmful for living creatures.
45-64 You have talked about the physico-chemical properties of pyrolysis oil and made comparison with diesel and gasoline. When talking about viscosity and density, in order to evaluate these properties, it is necessary to specify the temperature at which they were measured.
Viscosity was measured at 20°C with the aid of Brookfield DV-II+ viscometer. We have added this information to the text.
69-99 you have talked about the use of pyrolysis oil in diesel engines, then, (100-122) you have talked about its use in spark ignition engines, then, again about diesel ... I think the bibliographic study should be structured, on the two types of engines and presented separate arguments and studies.
We have modified the structure of this section exposing SI and CI engines separately. However, the literature on pyrolysis oil combustion in both these engines is really poor. My group is one of leading teams in this field.
125-126 I think it should be specified exactly what engine performance you intend to study in this paper.
The indicated mean effective pressure (IMEP) is the parameter that allows comparison between engines characterizing with various power, displacement, torque, speed, fuel consumption etc. etc. Therefore, the engine performance was expressed by IMEP in this manuscript.
137 Table 2 containing the properties of pyrolysis oil should be redone. I think a comparison of the properties for the three substances should be made: ethanol, pyrolysis oil and mixture.
According to your wish we modified table 2, inserting there properties as you suggested.
I think it is important to specify how you measured the physical properties and chemical composition of the pyrolysis oil, what devices and what methods you used.
Elemental analysis CHN was made with the aid of Leco CHN628. A sample was buried in pure oxygen at 950°C. Higher heating value was determined with the aid of LECO AC500. GC+MS Agilent, in Split mode was used to determine several specific compounds. We have added this information to the text.
139 Table 3 - I think needs to be rewritten. It is hard to read. The tests should be described in more detail.
Table 3 shows main parameters for engine tests as follows: lambda, load, crank shaft speed, spark timing and fuels applied for tests and technical specs of the engine. This is a complete list of parameters affecting combustion process in the engine and there is no need to present additional information that cannot be related to the main topic.
181, 217 there are two tables 5.
Sorry, it was corrected.
214, 228 fig 2, 3 - I don't understand why the trend of parameter variation has to be traced. Explain, please!
Parameters depicted in Fig.2 and 3 are parameters reflecting combustion issues in the IC engine and they are usually considered as the most important at all experimental works focused on thermodynamic analyses of combustion process there. Pmax vs. spark timing was to show sensivity of ST on Pmax, that affects bearings loads. IMEP vs. ST was to find out optimal ST, that was used for further analysis regarding combustion phases. COV of IMEP is determined to express repeatability of consecutive combustion events. As seen, the trend lines show that combustion of ethanol+PO appears as less stable in comparison with pure ethanol.
232…. paragraph 3.2. I think the mathematical model should be presented. The authors made only a story; the equations that were used should be presented.
The model for heat releasing during combustion in the engine is well-known among all engine researchers and engineers. This analytical equations (the mathematical model) is obligatory lectured in all students-level teaching courses related to IC engine thermodynamic theory. However, you are right, and we have added the final equations for HRR and MFB.
309-313 Finally, why was the 3: 1 oil-ethanol mixture used? Why is this report recommended? I believe that a comparative analysis of several mixing percentages should be made.
Yes. The analysis of various mixing percentage is a brilliant idea. It was already done and is a content for another manuscript submitted, so, there is no need to repeat it here, as it can be considered as auto-plagiarism then. Percentage of 75:25 (3:1) was selected regarding the fraction for pyrolysis oil, that was accepted for non-knocking combustion. So, this is main reason for the ratio 3:1. Pyrolysis oil is prone to generate combustion knock, therefore researchers usually apply it for the CI engine rather than the SI one. We wanted to show that PO can be combusted without knocking symptoms in the SI engine, but it should be blended with other fuel, in this case, with ethanol.
Taking into consideration the above, I propose to accept the manuscript with the major changes.
Reviewer 2 Report
This paper deals with the use of pyrolysis oil (PO) added to ethanol in an internal combustion spark ignition engine. The paper is very clear and with a logic thread, however, in my opinion, some results on engine out emissions should have been reported in order to give a complete analysis of the phenomena.
Remarks:
The concept of PO cetane number and the topic of its use in internal combustion engines is presented after the reference to [9] and [10] where PO has been considered as promising alternative fuel for internal combustion engines. The Introduction should be rearranged taking into according to this point.
The fuel used for the tests is a blend of ethanol and pyrolysis oil at ratio of 3:1, the authors should explain the way they selected this composition.
The fuel injection system description is not completely clear, please try to rephrase the sentence in lines 146-147
In Table 5. Next to each component specification indintity measured according with the text at lines 176-178
Minor comments
All the acronyms should be defined the first time they are used. Many acronyms are repeated multiple times and then also reported in the list, i.e.:
spark-ignition (SI) is reported at line 70 and 109
IMEP at line 134 and 186
Some other acronyms are defined too late, for example Crank Angle is used several times and the abbreviation CA is reported only at line 189
Table 4 is introduced twice: lines 143 and 172
Author Response
Comments and Suggestions for Authors
This paper deals with the use of pyrolysis oil (PO) added to ethanol in an internal combustion spark ignition engine. The paper is very clear and with a logic thread, however, in my opinion, some results on engine out emissions should have been reported in order to give a complete analysis of the phenomena.
Remarks:
The concept of PO cetane number and the topic of its use in internal combustion engines is presented after the reference to [9] and [10] where PO has been considered as promising alternative fuel for internal combustion engines. The Introduction should be rearranged taking into according to this point.
We have rearranged the introduction section with respect to your remark. Although, PO can be considered as a promising fuel for the compression ignition (CI) engine as regards its cetane number, but PO features with problems with solubility in diesel fuel, but can be soluble in alcohols. Hence, as the fuel for CI engines it can be considered as a single fuel, which can cause several problems with coking around injector’s nozzles. We observed it in our own research work. Therefore we decided to do investigation in a spark ignition engine.
The fuel used for the tests is a blend of ethanol and pyrolysis oil at ratio of 3:1, the authors should explain the way they selected this composition.
Let us just simply repeat the explanation from our response to another reviewer. The analysis of various mixing percentage was already done and is a content for another manuscript submitted, so, there is no need to repeat it here, as it can be considered as auto-plagiarism then. Percentage of 75:25 (3:1) was selected regarding the fraction for pyrolysis oil, that was accepted for non-knocking combustion. So, this is main reason for the ratio 3:1. Pyrolysis oil is prone to generate combustion knock, therefore researchers usually apply it for the CI engine rather than the SI one. We wanted to show that PO can be combusted without knocking symptoms in the SI engine, but it should be blended with other fuel, in this case, with ethanol.
The fuel injection system description is not completely clear, please try to rephrase the sentence in lines 146-147
We modified it as: A PFI (Port Fuel Injection) system with an injector mounted in the intake manifold was used. The fuel pump was replaced with a fuel buffer tank with constant internal pressure from the compressor's pneumatic system. This solution provides more stable fuel doses.
In Table 5. Next to each component specification indintity measured according with the text at lines 176-178
We are sorry that cannot answer to this remark. We do not know what did you mean writing “indintity”.
Minor comments
All the acronyms should be defined the first time they are used. Many acronyms are repeated multiple times and then also reported in the list, i.e.:
spark-ignition (SI) is reported at line 70 and 109
IMEP at line 134 and 186
Some other acronyms are defined too late, for example Crank Angle is used several times and the abbreviation CA is reported only at line 189
Table 4 is introduced twice: lines 143 and 172
We have corrected all these shortcomings.
Reviewer 3 Report
The paper needs substantial modification to be accepted for publications
1-For "48-55" references are essential
2-In Results and Discussions
provide the findings/ contributions and analysis
don't use sentence like
The results from the investigation are divided into 3 thematic issues as follows:
3-The sentence 264-265
"When one correlates the plot IMEP 264vs. spark timing (Figure 2b) with the plot CA50 vs. spark timing (Figure 5b) then he obtains the plot depicted in Figure 6, presenting correlation between CA50 and IMEP
is misleading
4-Existing knowledge from literature need not be added in the conclusion.
Kindly remove
"As known from literature survey, pyrolysis oil as the only fuel can be applied to a compression ignition engine due to good tendency for its self-ignition. However, pyrolysis oil can be also combusted in the spark-ignition engine."
5-Make a comprehensive proof reading, sentences are not framed appropriately in many occasion.
Also add some recent references
Author Response
Comments and Suggestions for Authors
The paper needs substantial modification to be accepted for publications
1-For "48-55" references are essential
The reference for these statements has been added.
2-In Results and Discussions
provide the findings/ contributions and analysis
don't use sentence like
The results from the investigation are divided into 3 thematic issues as follows:
The contributions/findings and analysis were provided. The statement was removed.
3-The sentence 264-265
"When one correlates the plot IMEP 264vs. spark timing (Figure 2b) with the plot CA50 vs. spark timing (Figure 5b) then he obtains the plot depicted in Figure 6, presenting correlation between CA50 and IMEP is misleading
It was rearranged to the form: “Based on data presented in Figures 2b and 5b, the graph CA50 and IMEP is depicted in Figure 6.”
4-Existing knowledge from literature need not be added in the conclusion.
Kindly remove
"As known from literature survey, pyrolysis oil as the only fuel can be applied to a compression ignition engine due to good tendency for its self-ignition. However, pyrolysis oil can be also combusted in the spark-ignition engine."
It was reworded.
5-Make a comprehensive proof reading, sentences are not framed appropriately in many occasion.
Also add some recent references
We did our best regarding the prof reading. Furthermore, we added a few references as inserted below, but it was to prove that majority of papers deals with combustion of pyrolysis oil in the diesel engine. Our research work on the spark ignition engine can be considered as a new one among the papers published.
References added:
Marinangelli, R.; Boldingh, E.; Cabanban, S.; Fei, S.; Ellis, G.; Bain, R.; Hsu, G.; Elliott, D. C. Pyrolysis Oil to Gasoline-Final Report, PNNL-19053. 2009, No. December, undefined-undefined.
Hossain, A. K.; Davies, P. A. Pyrolysis Liquids and Gases as Alternative Fuels in Internal Combustion Engines - A Review. Renewable and Sustainable Energy Reviews. Pergamon May 1, 2013, pp 165–189. https://doi.org/10.1016/j.rser.2012.12.031.
Kim, T. Y.; Lee, S. H. Combustion and Emission Characteristics of Wood Pyrolysis Oil-Butanol Blended Fuels in a DI Diesel Engine. Int. J. Automot. Technol. 2015, 16 (6), 903–912. https://doi.org/10.1007/s12239-015-0092-4.
Tomar, M.; Jain, A.; Pujari, P. C.; Dewal, H.; Kumar, N. Potentials of Waste Plastic Pyrolysis Oil as an Extender Fuel for Diesel Engine. Arab. J. Geosci. 2020, 13 (13), 1–10. https://doi.org/10.1007/s12517-020-05574-6.
Mikulski, M.; Ambrosewicz-Walacik, M.; Hunicz, J.; Nitkiewicz, S. Combustion Engine Applications of Waste Tyre Pyrolytic Oil. Progress in Energy and Combustion Science. Elsevier Ltd July 1, 2021, p 100915. https://doi.org/10.1016/j.pecs.2021.100915.
Zahir Hussain, A.; Santhoshkumar, A.; Ramanathan, A. Assessment of Pyrolysis Waste Engine Oil as an Alternative Fuel Source for Diesel Engine. J. Therm. Anal. Calorim. 2020, 141 (6), 2277–2293. https://doi.org/10.1007/s10973-020-09516-y.
Karagoz, M.; Uysal, C.; Agbulut, U.; Saridemir, S. Energy, Exergy, Economic and Sustainability Assessments of a Compression Ignition Diesel Engine Fueled with Tire Pyrolytic Oil−diesel Blends. J. Clean. Prod. 2020, 264, 121724. https://doi.org/10.1016/j.jclepro.2020.121724.
Round 2
Reviewer 1 Report
Dear Authors,
Very briefly, you can find below, some of my specific comments on both the form and the substance of your manuscript:
Very briefly, you can find below, some of my specific comments on your manuscript:
10– 13 In the abstract of the paper you said that pyrolysis oil is extremely harmful substance, and it must be neutralized. So, if it is harmful, it should not be used as fuel, and its neutralization is not mentioned in the text. So, I would like a deeper argumentation of the advantages of using this fuel / additive.
Fuels for IC engines usually are hydrocarbons (CxHy) and oxygenated hydrocarbons CxHyOz. All of them are considered as harmful for living creatures. Pyrolysis oil is just a mixture of various hydrocarbons. At combustion it is oxidized to water and CO2. There might be some trace concentrations of sulphur that is oxidized to SOx. Hence, combustion products, except SOx, cannot be managed as harmful for both natural environment and humans and combustion can be treated as the best neutralization technology in this case. With respect to your remark, you are right. We added to the statement that pyrolysis oil is particularly harmful for living creatures.
Acceptable answer
45-64 You have talked about the physico-chemical properties of pyrolysis oil and made comparison with diesel and gasoline. When talking about viscosity and density, in order to evaluate these properties, it is necessary to specify the temperature at which they were measured.
Viscosity was measured at 20°C with the aid of Brookfield DV-II+ viscometer. We have added this information to the text.
I referred to the values presented in paragraph 1 Introduction, taken from references. In standards is presented Viscosity @ 40 °C. If you compare the viscosity of the oil measured at 20 with that of diesel at 40… obviously, it is not ok. You probably didn't get the full information from that reference.
69-99 you have talked about the use of pyrolysis oil in diesel engines, then, (100-122) you have talked about its use in spark ignition engines, then, again about diesel ... I think the bibliographic study should be structured, on the two types of engines and presented separate arguments and studies.
We have modified the structure of this section exposing SI and CI engines separately. However, the literature on pyrolysis oil combustion in both these engines is really poor. My group is one of leading teams in this field.
Sorry, I didn't see the modification.
125-126 I think it should be specified exactly what engine performance you intend to study in this paper.
The indicated mean effective pressure (IMEP) is the parameter that allows comparison between engines characterizing with various power, displacement, torque, speed, fuel consumption etc. etc. Therefore, the engine performance was expressed by IMEP in this manuscript.
Acceptable answer
137 Table 2 containing the properties of pyrolysis oil should be redone. I think a comparison of the properties for the three substances should be made: ethanol, pyrolysis oil and mixture.
According to your wish we modified table 2, inserting there properties as you suggested.
Acceptable answer
I think it is important to specify how you measured the physical properties and chemical composition of the pyrolysis oil, what devices and what methods you used.
Elemental analysis CHN was made with the aid of Leco CHN628. A sample was buried in pure oxygen at 950°C. Higher heating value was determined with the aid of LECO AC500. GC+MS Agilent, in Split mode was used to determine several specific compounds. We have added this information to the text.
Acceptable answer even if the specification is very limited.
139 Table 3 - I think needs to be rewritten. It is hard to read. The tests should be described in more detail.
Table 3 shows main parameters for engine tests as follows: lambda, load, crank shaft speed, spark timing and fuels applied for tests and technical specs of the engine. This is a complete list of parameters affecting combustion process in the engine and there is no need to present additional information that cannot be related to the main topic.
I understand, it remains a matter of form: why don't you put the values on two columns, as in the line above (in the preliminary test)?
181, 217 there are two tables 5.
Sorry, it was corrected.
OK
214, 228 fig 2, 3 - I don't understand why the trend of parameter variation has to be traced. Explain, please!
Parameters depicted in Fig.2 and 3 are parameters reflecting combustion issues in the IC engine and they are usually considered as the most important at all experimental works focused on thermodynamic analyses of combustion process there. Pmax vs. spark timing was to show sensivity of ST on Pmax, that affects bearings loads. IMEP vs. ST was to find out optimal ST, that was used for further analysis regarding combustion phases. COV of IMEP is determined to express repeatability of consecutive combustion events. As seen, the trend lines show that combustion of ethanol+PO appears as less stable in comparison with pure ethanol.
Acceptable answer
232…. paragraph 3.2. I think the mathematical model should be presented. The authors made only a story; the equations that were used should be presented.
The model for heat releasing during combustion in the engine is well-known among all engine researchers and engineers. This analytical equations (the mathematical model) is obligatory lectured in all students-level teaching courses related to IC engine thermodynamic theory. However, you are right, and we have added the final equations for HRR and MFB.
OK
309-313 Finally, why was the 3: 1 oil-ethanol mixture used? Why is this report recommended? I believe that a comparative analysis of several mixing percentages should be made.
Yes. The analysis of various mixing percentage is a brilliant idea. It was already done and is a content for another manuscript submitted, so, there is no need to repeat it here, as it can be considered as auto-plagiarism then. Percentage of 75:25 (3:1) was selected regarding the fraction for pyrolysis oil, that was accepted for non-knocking combustion. So, this is main reason for the ratio 3:1. Pyrolysis oil is prone to generate combustion knock, therefore researchers usually apply it for the CI engine rather than the SI one. We wanted to show that PO can be combusted without knocking symptoms in the SI engine, but it should be blended with other fuel, in this case, with ethanol.
For a better understanding of the subject, I think you should make a brief explanation in the text, without being accused of self-plagiarism.
Author Response
Reviewer #1
45-64 You have talked about the physico-chemical properties of pyrolysis oil and made comparison with diesel and gasoline. When talking about viscosity and density, in order to evaluate these properties, it is necessary to specify the temperature at which they were measured.
Viscosity was measured at 20°C with the aid of Brookfield DV-II+ viscometer. We have added this information to the text.
I referred to the values presented in paragraph 1 Introduction, taken from references. In standards is presented Viscosity @ 40 °C. If you compare the viscosity of the oil measured at 20 with that of diesel at 40… obviously, it is not ok. You probably didn't get the full information from that reference.
Ans. The reference did not provide the temperature. We have measured viscosity of similar like pyros oil with our own viscometer as mentioned. Unfortunately, we did it at room temperature of 20°C, but later we repeated the test at 40°C, and it does not change significantly. @ 20°C – 2.65 cSt, @40°C – 2.53 cSt. So, the difference is less than 5%. But to be honest we deleted the numbers from the text.
69-99 you have talked about the use of pyrolysis oil in diesel engines, then, (100-122) you have talked about its use in spark ignition engines, then, again about diesel ... I think the bibliographic study should be structured, on the two types of engines and presented separate arguments and studies.
We have modified the structure of this section exposing SI and CI engines separately. However, the literature on pyrolysis oil combustion in both these engines is really poor. My group is one of leading teams in this field.
Sorry, I didn't see the modification.
Ans. We have structured the text to separate sections for SI and CI engines.
309-313 Finally, why was the 3: 1 oil-ethanol mixture used? Why is this report recommended? I believe that a comparative analysis of several mixing percentages should be made.
Yes. The analysis of various mixing percentage is a brilliant idea. It was already done and is a content for another manuscript submitted, so, there is no need to repeat it here, as it can be considered as auto-plagiarism then. Percentage of 75:25 (3:1) was selected regarding the fraction for pyrolysis oil, that was accepted for non-knocking combustion. So, this is main reason for the ratio 3:1. Pyrolysis oil is prone to generate combustion knock, therefore researchers usually apply it for the CI engine rather than the SI one. We wanted to show that PO can be combusted without knocking symptoms in the SI engine, but it should be blended with other fuel, in this case, with ethanol.
For a better understanding of the subject, I think you should make a brief explanation in the text, without being accused of self-plagiarism.
Ans. We have added short sentence in this matter. Thank you for your work on reviewing the manuscript.
Reviewer 2 Report
The authors made some changes in the paper according to the reviewers’ comments.
About my question:
The fuel used for the tests is a blend of ethanol and pyrolysis oil at ratio of 3:1, the authors should explain the way they selected this composition.
The reply was:
Let us just simply repeat the explanation from our response to another reviewer. The analysis of various mixing percentage was already done and is a content for another manuscript submitted, so, there is no need to repeat it here, as it can be considered as auto-plagiarism then. Percentage of 75:25 (3:1) was selected regarding the fraction for pyrolysis oil, that was accepted for non-knocking combustion. So, this is main reason for the ratio 3:1. Pyrolysis oil is prone to generate combustion knock, therefore researchers usually apply it for the CI engine rather than the SI one. We wanted to show that PO can be combusted without knocking symptoms in the SI engine, but it should be blended with other fuel, in this case, with ethanol.
I understand the authors do not want to report exactly the same work already done, but a short resume of the outcomes of the previous work, together with the reference, should be reported to explain the reason why the proportion 3:1 was chosen.
Author Response
I understand the authors do not want to report exactly the same work already done, but a short resume of the outcomes of the previous work, together with the reference, should be reported to explain the reason why the proportion 3:1 was chosen.
Ans. Percentage of 75:25 (3:1) was selected regarding the fraction for pyrolysis oil, that was accepted for non-knocking combustion. So, this is main reason for the ratio 3:1. Pyrolysis oil is prone to generate combustion knock, therefore researchers usually apply it for the CI engine rather than the SI one. We wanted to show that PO can be combusted without knocking symptoms in the SI engine, but it should be blended with other fuel, in this case, with ethanol.
We have added short sentence in this matter. Thank you for your work on reviewing the manuscript.
Reviewer 3 Report
The paper still needs few changes to be accepted for publication;
1-Reference can be provided for the statements
"Pyrolysis oil (PO) is a black liquid substance obtained from thermal processing of organic substance at the temperature of 350-600°C without oxygen"
2-The authors can provide sp. energy of PO also ethanol/methanol
can refer the Table-1 of following paper
https://www.mdpi.com/1996-1073/13/22/5879/htm
3-Kindly make comprehensive proofreading to improve the English style and standard.
Author Response
1-Reference can be provided for the statements
"Pyrolysis oil (PO) is a black liquid substance obtained from thermal processing of organic substance at the temperature of 350-600°C without oxygen"
Ans. The raw pyrolysis oil just look as described. It is obvious for all researchers experienced a little bit in pyrolysis process.
2-The authors can provide sp. energy of PO also ethanol/methanol can refer the Table-1 of following paper https://www.mdpi.com/1996-1073/13/22/5879/htm
Ans. This article corresponds to neither pyrolysis nor IC engines. So, we cannot see any reason for adding it to the references. Thanks.
3-Kindly make comprehensive proofreading to improve the English style and standard.
Ans. As we are not native English speaking researchers, we did our best to make the proofreading and improve English style. Thank you for your work on reviewing the manuscript.
