Interactions of Arbuscular Mycorrhizal Fungi with Hyphosphere Microbial Communities in a Saline Soil: Impacts on Phosphorus Availability and Alkaline Phosphatase Gene Abundance
Abstract
:1. Introduction
2. Materials and methods
2.1. Soil
2.2. AMF Inoculum and Plant Host
2.3. Experimental Design and Growth Conditions
2.4. Root Staining and AMF Colonization
2.5. Soil Extractable P and Plant Shoot P Concentration
2.6. Phosphatase Gene Quantitation in Hyphosphere Soil
2.7. Alkaline Phosphatase Enzyme Assay
2.8. Statistical Analysis
3. Results
3.1. Root Colonization Effects of Experimental Treatments
3.2. Plant Available P in Hyphosphere Soil and Its Uptake by Plants
3.3. Alkaline Phosphatase Gene (phoD) and Microbial Community Abundance in the Hyphosphere
3.4. Alkaline Phosphatase Enzyme Assay
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | Value |
---|---|
pH | 8.5 |
EC (saturated extract, dS/m) | 6.32 |
P (Mehlich-3, mg/kg) Nitrate-N (mg/kg) Potassium (mg/kg) | 45 26 614 |
Organic matter content (%) | 0.34 |
Clay (%) | 46.7 |
Silt (%) | 20.7 |
Sand (%) | 32.6 |
AMF Inoculum | Treatment | % Root Colonized by AMF |
---|---|---|
None | IS-OS | 0 ± 0 e |
IS-OU | 2.91 ± 2.6 ecd | |
IU-OS | 0.05 ± 0.08 e | |
IU-OU | 1.95 ± 1.5 ed | |
Fm | IS-OS | 25.9 ± 15.1 ba |
IS-OU | 15.30 ± 6.2 bc | |
IU-OS | 14.47 ± 7.6 bcd | |
IU-OU | 30.82 ± 10.7 a |
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Masrahi, A.; Somenahally, A.; Gentry, T. Interactions of Arbuscular Mycorrhizal Fungi with Hyphosphere Microbial Communities in a Saline Soil: Impacts on Phosphorus Availability and Alkaline Phosphatase Gene Abundance. Soil Syst. 2020, 4, 63. https://doi.org/10.3390/soilsystems4040063
Masrahi A, Somenahally A, Gentry T. Interactions of Arbuscular Mycorrhizal Fungi with Hyphosphere Microbial Communities in a Saline Soil: Impacts on Phosphorus Availability and Alkaline Phosphatase Gene Abundance. Soil Systems. 2020; 4(4):63. https://doi.org/10.3390/soilsystems4040063
Chicago/Turabian StyleMasrahi, Abdurrahman, Anil Somenahally, and Terry Gentry. 2020. "Interactions of Arbuscular Mycorrhizal Fungi with Hyphosphere Microbial Communities in a Saline Soil: Impacts on Phosphorus Availability and Alkaline Phosphatase Gene Abundance" Soil Systems 4, no. 4: 63. https://doi.org/10.3390/soilsystems4040063