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Volume 74
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10.5603/ARM.28079
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Advances in Respiratory Medicine is published by MDPI from Volume 90 Issue 4 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Via Medica.
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Article

Identification of Mycobacteriaceae Species Based on the Hsp-65 Gene Polymorphism Analysis by PCR–RFLP

by
Adam Frangrat
*,
Renata Walkiewicz
,
Aleksandra Safianowska
,
Hanna Grubek-Jaworska
and
Ryszarda Chazan
Katedra i Klinika Chorób Wewnętrznych, Pneumonologii i Alergologii, AM w Warszawie, Warsaw, Poland
*
Author to whom correspondence should be addressed.
Adv. Respir. Med. 2006, 74(1), 95-100; https://doi.org/10.5603/ARM.28079
Submission received: 1 March 2006 / Revised: 1 March 2006 / Accepted: 1 March 2006 / Published: 1 March 2006

Abstract

The polymorphism of the short fragment of the heat shock protein 65 encoding gene was evaluated by the PCR–RFLP technique described by Telenti and further developed by Devallois for identification of mycobac-terial species in routine laboratory work. We analysed 58 strains representing 25 different mycobacterial species (24 reference strains and 34 clinical isolates). The results obtained by PCR-RFLP and HPLC identification techniques were highly concordant The results were compatible for 87.5% (21/24) reference strains and for 97.1% (33/34) clinical isolates. The PCR–RFLP method allowed for accurate identification mycobacterial species, especially pathogenic strains. Restriction patterns obtained for 25 species of Mycobacteriaceae genus could help in constructing the data base and algorithms used in routine laboratory practice.
Keywords: tuberculous mycobacteria; atypical mycobacteria; PCR–RFLP; HPLC tuberculous mycobacteria; atypical mycobacteria; PCR–RFLP; HPLC

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MDPI and ACS Style

Frangrat, A.; Walkiewicz, R.; Safianowska, A.; Grubek-Jaworska, H.; Chazan, R. Identification of Mycobacteriaceae Species Based on the Hsp-65 Gene Polymorphism Analysis by PCR–RFLP. Adv. Respir. Med. 2006, 74, 95-100. https://doi.org/10.5603/ARM.28079

AMA Style

Frangrat A, Walkiewicz R, Safianowska A, Grubek-Jaworska H, Chazan R. Identification of Mycobacteriaceae Species Based on the Hsp-65 Gene Polymorphism Analysis by PCR–RFLP. Advances in Respiratory Medicine. 2006; 74(1):95-100. https://doi.org/10.5603/ARM.28079

Chicago/Turabian Style

Frangrat, Adam, Renata Walkiewicz, Aleksandra Safianowska, Hanna Grubek-Jaworska, and Ryszarda Chazan. 2006. "Identification of Mycobacteriaceae Species Based on the Hsp-65 Gene Polymorphism Analysis by PCR–RFLP" Advances in Respiratory Medicine 74, no. 1: 95-100. https://doi.org/10.5603/ARM.28079

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