Excretion Dynamics of Arboviruses in Mosquitoes and the Potential Use in Vector Competence Studies and Arbovirus Surveillance
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Mosquito Infection and Examination of Vector Competence
2.3. Collection of Mosquito Excreta
2.4. Nucleic Acid Extractions and Analysis
2.5. Vector Competence Indices and Infection Status
2.6. Statistical Analysis
3. Results
3.1. Vector Competence Trials
3.2. Excretion of Viral RNA via the Infectious Blood Meal
3.3. Dependence of Viral Shedding on Mosquito Infection Status
3.4. Correlation between Amount of Viral RNA in Excreta and Viral Load in Mosquitoes
3.5. Duration of Viral Shedding after Infection
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Virus Strain | GenBank Accession No. | Titer 1 |
---|---|---|
WNV lineage 1 (Italy, 2008) | JF719066 | 5.62 × 107 |
WNV lineage 2 (Germany, 2018) | MH924836 | 1.33 × 109 |
USUV lineage Europe 3 (Germany, 2011) | HE599647 | 5.01 × 107 |
USUV lineage Europe 3 (Germany, 2016) | KY084498 | 5.62 × 107 |
TBEV Western Neudoerfl | U27495 | 1.00 × 108 |
Experiment No. | Virus | Mosquito Species | Incubation Period (Days) | Number of Mosquitoes per Incubation Chamber | Material Used for Excreta Collection |
---|---|---|---|---|---|
#1 | WNV lineage 1 (Italy, 2008) | AeV | 14 | 1 or 5 | FTA cards; Parafilm and 1 × PBS |
#2 | WNV lineage 1 (Italy, 2008) | CxM | 20 | 5 | FTA cards; Parafilm and 1 × PBS |
#3 | WNV lineage 2 (Germany, 2018) | AeV | 14 | 1 | Parafilm and AVL buffer |
#4 | TBEV Western Neudoerfl | CxM | 14 | 1 | Parafilm and AVL buffer |
#5 | USUV lineage Europe 3 (Germany, 2016) | CxM | 14 | 1 | Parafilm and AVL buffer; only if excreta were visible |
#6 | USUV lineage Europe 3 (Germany, 2011) | CxM | 14 | 1 | Parafilm and AVL buffer; only if excreta were visible |
#7 | WNV lineage 2 (Germany, 2018) | CxM | 14 | 1 | Parafilm and AVL buffer; only if excreta were visible |
Experiment No. | Virus | Mosquito Species | Blood Meal Titer 1 | Mosquitoes Examined 2 | IR % (n/n) | DR % (n/n) | TR % (n/n) |
---|---|---|---|---|---|---|---|
#1 | WNV lineage 1 (Italy, 2008) | AeV | 6.125 | 10 | 0 (0/10) | N/A | N/A |
#2 | WNV lineage 1 (Italy, 2008) | CxM | 5.500 | 2 | 0 (0/2) | N/A | N/A |
#3 | WNV lineage 2 (Germany, 2018) | AeV | 7.438 | 8 | 0 (0/8) | N/A | N/A |
#4 | TBEV Western Neudoerfl | CxM | 7.063 | 24 | 0 (0/24) | N/A | N/A |
#5 | USUV lineage Europe 3 (Germany, 2016) | CxM | 6.063 | 18 | 5.56 (1/18) | 0 (0/1) | N/A |
#6 | USUV lineage Europe 3 (Germany, 2011) | CxM | 7.125 | 16 | 100.00 (16/16) | 75.00 (12/16) | 83.33 (10/12) |
#7 | WNV lineage 2 (Germany, 2018) | CxM | 6.844 | 14 | 78.54 (11/14) | 81.82 (9/11) | 77.78 (7/9) |
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Körsten, C.; Vasić, A.; AL-Hosary, A.A.; Tews, B.A.; Răileanu, C.; Silaghi, C.; Schäfer, M. Excretion Dynamics of Arboviruses in Mosquitoes and the Potential Use in Vector Competence Studies and Arbovirus Surveillance. Trop. Med. Infect. Dis. 2023, 8, 410. https://doi.org/10.3390/tropicalmed8080410
Körsten C, Vasić A, AL-Hosary AA, Tews BA, Răileanu C, Silaghi C, Schäfer M. Excretion Dynamics of Arboviruses in Mosquitoes and the Potential Use in Vector Competence Studies and Arbovirus Surveillance. Tropical Medicine and Infectious Disease. 2023; 8(8):410. https://doi.org/10.3390/tropicalmed8080410
Chicago/Turabian StyleKörsten, Christin, Ana Vasić, Amira A. AL-Hosary, Birke A. Tews, Cristian Răileanu, Cornelia Silaghi, and Mandy Schäfer. 2023. "Excretion Dynamics of Arboviruses in Mosquitoes and the Potential Use in Vector Competence Studies and Arbovirus Surveillance" Tropical Medicine and Infectious Disease 8, no. 8: 410. https://doi.org/10.3390/tropicalmed8080410