A Practical Peptide Synthesis Workflow Using Amino-Li-Resin
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials and Reagents
2.2. Instrumentation
2.3. Synthesis Protocol
2.3.1. Loading Determination
2.3.2. Resin Swelling
2.3.3. Mixing and Filtration Strategy
2.3.4. Addition of Fmoc Rink-Amide Linker
2.3.5. Fmoc-AA-OH Coupling
Deprotection
Coupling
Cleavage
Precipitation
3. Result
Yield and Purity
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Akintayo, D.C.; Manne, S.R.; de la Torre, B.G.; Li, Y.; Albericio, F. A Practical Peptide Synthesis Workflow Using Amino-Li-Resin. Methods Protoc. 2022, 5, 72. https://doi.org/10.3390/mps5050072
Akintayo DC, Manne SR, de la Torre BG, Li Y, Albericio F. A Practical Peptide Synthesis Workflow Using Amino-Li-Resin. Methods and Protocols. 2022; 5(5):72. https://doi.org/10.3390/mps5050072
Chicago/Turabian StyleAkintayo, Damilola Caleb, Srinivasa Rao Manne, Beatriz G. de la Torre, Yongfu Li, and Fernando Albericio. 2022. "A Practical Peptide Synthesis Workflow Using Amino-Li-Resin" Methods and Protocols 5, no. 5: 72. https://doi.org/10.3390/mps5050072