Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTipTM Probes: Application to the Pru p 3-Phytosphingosine System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Thiol Modification of DeepTipTM Probes and MicroDeckTM Substrates
2.3. Decoration of the DeepTipTM Probes with Phytosphingosine
2.4. Modification of Pru p 3 with Sulfo-LC-SPDP and Decoration of the MicroDeckTM Substrates
2.5. Determination of the Pru p 3-phytosphingosine Force–Distance (F-d) Curves
3. Results and Discussion
3.1. Classification of the Force–Distance (F-d) Curves
3.2. Interpretation of the Force–Distance (F-d) Curves
3.3. New Perspectives Opened by High-Yield A-AFM
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Curve | X2 | χ20.05 (f = 9) |
---|---|---|
Single elastomeric | 12.2 | 15.51 |
First peak —two-peak elastomeric | 13.2 | |
Second peak —two-peak elastomeric | 2.7 |
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Daza, R.; Garrido-Arandia, M.; Corregidor-Ortiz, D.; Pérez, C.I.; Colchero, L.; Tabraue-Rubio, R.; Elices, M.; Guinea, G.V.; Diaz-Perales, A.; Pérez-Rigueiro, J. Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTipTM Probes: Application to the Pru p 3-Phytosphingosine System. Biomimetics 2023, 8, 595. https://doi.org/10.3390/biomimetics8080595
Daza R, Garrido-Arandia M, Corregidor-Ortiz D, Pérez CI, Colchero L, Tabraue-Rubio R, Elices M, Guinea GV, Diaz-Perales A, Pérez-Rigueiro J. Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTipTM Probes: Application to the Pru p 3-Phytosphingosine System. Biomimetics. 2023; 8(8):595. https://doi.org/10.3390/biomimetics8080595
Chicago/Turabian StyleDaza, Rafael, María Garrido-Arandia, Daniel Corregidor-Ortiz, Carla Isabel Pérez, Luis Colchero, Raquel Tabraue-Rubio, Manuel Elices, Gustavo V. Guinea, Araceli Diaz-Perales, and José Pérez-Rigueiro. 2023. "Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTipTM Probes: Application to the Pru p 3-Phytosphingosine System" Biomimetics 8, no. 8: 595. https://doi.org/10.3390/biomimetics8080595