Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality
by
, , , , and
Milica Aćimović
1
,
Biljana Lončar
2,*
,
Jovana Stanković Jeremić
3, 
Mirjana Cvetković
3,
Lato Pezo
4,
Milada Pezo
5,
Marina Todosijević
6 and
Vele Tešević
6 1
Institute of Field and Vegetable Crops Novi Sad—National Institute of the Republic of Serbia, Maksima Gorkog 30, 21000 Novi Sad, Serbia
2
Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
3
Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, 11000 Belgrade, Serbia
4
Institute of General and Physical Chemistry, Studentski trg 10–12, 11000 Belgrade, Serbia
5
Department of Thermal Engineering and Energy, “Vinča” Institute of Nuclear Sciences—National Institute of the Republic of Serbia, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia
6
Department of Organic Chemistry, Faculty of Chemistry, Studentski trg 16, 11000 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Horticulturae 2022, 8(4), 281; https://doi.org/10.3390/horticulturae8040281
Submission received: 2 March 2022
/
Revised: 24 March 2022
/
Accepted: 25 March 2022
/
Published: 27 March 2022
(This article belongs to the Special Issue Medicinal, Aromatic, Spice Plants: Biodiversity, Phytochemistry, Bioactivity and Their Processing Innovation)
Abstract
:Lavandula sp. essential oil and hydrolate are commercially valuable in various industry branches with the potential for wide-ranging applications. This study aimed to evaluate the quality of these products obtained from L. x intermedia cv. ‘Budrovka’ for the first time cultivated on Fruška Gora Mt. (Serbia) during three successive seasons (2019, 2020, and 2021). Essential oil extraction was obtained by steam distillation, and the composition and influence of weather conditions were also assessed, using flowering tops. The obtained essential oils and hydrolates were analysed by gas chromatography with a flame ionization detector (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). A linear regression model was developed to predict L. x intermedia cv. ‘Budrovka’ essential oil volatile compound content and hydrolate composition during three years, according to temperature and precipitation data, and the appropriate regression coefficients were calculated, while the correlation analysis was employed to analyse the correlations in hydrolate and essential oil compounds. To completely describe the structure of the research data that would present a better insight into the similarities and differences among the diverse L. x intermedia cv. ‘Budrovka’ samples, the PCA was used. The most dominant in L. intermedia cv. ‘Budrovka’ essential oil and hydrolate were oxygenated monoterpenes: linalool, 1,8-cineole, borneol, linalyl acetate, and terpinene-4-ol. It is established that the temperature was positively correlated with all essential oil and hydrolate compounds. The precipitations were positively correlated with the main compounds (linalool, 1,8-cineole, and borneol), while the other compounds’ content negatively correlated to precipitation. The results indicated that Fruška Gora Mt. has suitable agro-ecological requirements for cultivating Lavandula sp. and providing satisfactory essential oil and hydrolate.
1. Introduction
The genus Lavandula (order Lamiales, family Lamiaceae) originates from the Mediterranean. It is cultivated worldwide as an ornamental and essential oil-bearing plant [1,2]. There are 39 species, but only three are commercially important: true or English lavender (L. angustifolia Mill.), spike or Spanish lavender (L. latifolia Medic.), and their hybrid known as lavandin (L. x intermedia Emeric ex Loisel). With more than 400 registered cultivars and hybrids, Lavandula sp. cultivation, essential oil production, and consumption rapidly has increased due to certain profits [3,4,5,6]. Major producing regions of Lavandula sp. are in Europe, and the dominant countries are Bulgaria (with 3700 ha) and France (with 3500 ha) [1,3], but it is crucial in Mediterranean countries, especially Greece, Spain, and Turkey, as well [7]. Croatia is traditionally associated with Lavandula sp. cultivation and essential oil production on the Adriatic coast, but continental parts have become a commercial cultivation region [8,9]. In Hungary, a country with a continental climate, the cultivation of Lavandula sp. has expanded considerably in recent years [10], and in Romania [5]. Serbia follows the same path, with more than 100 ha under this crop in 2021, with an increasing tendency for the future (Aćimović, personal communication).
The Lavandula sp. essential oil is used in cosmetic (soaps, bath, colognes, perfumes, skin lotions, and after-shaves), pharmaceutical (mild sedative and analgesic, rubefacient and wound healing, and antioxidant and antimicrobial agents), and food industries (flavourings in baked goods, beverages, puddings, ice creams, candies, and chewing gums), in household products (detergents and hygiene products) and aromatherapy (for decreasing stress and anxiety, as well as pain intensity and against migraine), but it is also used as a biopesticide [3,4,11,12,13,14,15]. However, the application of lavender depends on its chemical composition. The common criteria for determining Lavandula sp. essential oil quality are camphor, linalool, and linalyl acetate percentage [4]. Essential oil of L. angustifolia is highly valued due to the low content of camphor (up to 1.2% according to European Pharmacopoeia) and is much more expensive. Therefore, it is often mixed with cheaper oils of L. latifolia and L. x intermedia to achieve better quality that satisfies ISO 8902 standard [15,16]. However, L. latifolia and L. x intermedia achieve higher essential oil yields [17,18]. Essential oil content and composition are primarily determined by plant genotype. However, it may be influenced by environmental factors (climate, but also weather conditions during growth year, soil conditions, and nutrient supply), harvest time, post-harvest treatments, and extraction methods [10,19,20,21].
Hydrolates are accrued as by-products during the steam distillation of essential oils. Essential oils contain volatile, lipid-soluble, and partially water-soluble compounds, generally of lower density than water [22,23]. However, volatile water-soluble compounds remain in condensate water in a Florentine flask (oil–water separator) and give a specific fragrance. This water is called hydrolate, hydrosol, aromatic or floral water [24,25,26,27]. Since hydrolates contain only a small amount of dissolved essential oil components in water, the amount of volatile organic compounds affects their biological properties [28,29]. Principally, they are by-products of essential oil distillation that could be useful as raw material in many industries, such as the food, and beverage industry (for flavouring and preservation, as well as in soft drinks), cosmetics (replacement for water phase in cosmetics, lotions, creams, soaps, and tonics) and aromatherapy (skincare or as massage products, as facial and body sprays to feel relaxed and refreshed, as air fresheners) [24]. Although the quality standards for hydrolates are not defined, their global economic impact is increasing [30]. Therefore, it is necessary to develop norms and standards for plant-derived components as there is growing interest in using them. Furthermore, due to volatile organic compounds, Lavandula sp. hydrolate has a pleasant lavender aroma and biological activities. Therefore, it has the potential for wide-ranging applications [28,29,31].
Fruška Gora Mt. (in Latin Alma Mons) is situated in northern Serbia, at the confluence of the Danube and Sava Rivers. This is the oldest national park in Serbia (with 25,525 ha of protected area), with almost 90% of the linden, oak, and beech forest. The rims of the Fruška Gora Mt. are used for grape cultivation, and this tradition dates from the Roman period. However, medicinal and aromatic plants were not cultivated in this region until recently. As a result, many wild species of medicinal plants, are mainly collected by gatherers [32]. This region is suitable for organic cultivation, and previous research indicated promising results in organically grown Lavandula sp. through Europe [33,34,35]. Lavandula sp. is a perennial, heliophyte, and drought-resistant shrub [10]. It requires well-drained soil, but it can adapt to poor soils with low fertility [36]. Croatian cultivar of L. x intermedia ‘Budrovka’ is widely grown in the former Yugoslavia region. This cultivar is well adapted to the continental climate and low temperatures during winter, up to –20 °C [37,38].
Considering the great commercial importance of Lavandula sp. flowers and essential oil in different industry branches and the increasing trend of cultivation lavender and lavandin through Europe, this paper aimed to evaluate the quality of introduced L. x intermedia cv. ‘Budrovka’ on Fruška Gora Mt. (Serbia). The main goal of this investigation was the influence of microclimatic conditions in the foreground temperature and precipitations in a selected location on L. x intermedia essential oil and hydrolate quality. The second goal of this investigation is to evaluate the essential oil quality of cv. ‘Budrovka’ compared to other samples L. x intermedia reported in the literature and ISO standards. Further, results of the chemical composition of L. x intermedia ‘Budrovka’ hydrolates from this study and other from literature were used for setting ranges of the main compounds of Lavandula sp. hydrolate.
2. Materials and Methods
2.1. Plant Material
The plantation of Lavandula x intermedia Emeric ex Loisel cv. ‘Budrovka’ was established in autumn 2014, at a certified organic farm in the village of Bukovac (area Beljevo; 45.111744 N, 19.530732 E) on Fruška Gora region (Figure 1). One-year-old seedlings were planted at a 1.5 m interrow distance and 0.5 m between plants.
2.2. Soil Characteristics
Basic chemical properties of the soil where L. x intermedia was cultivated were determined before establishing the experiment (October 2014) in samples of the top layer (0 to 40 cm) and are shown in Table 1. The soil samples were analysed at the Agriculture Extension Service Novi Sad, following standardized methods adopted in Serbia [39].
2.3. Weather Conditions
The climate of Fruška Gora, as an isolated mountain, significantly differs from its surroundings and modifies the local meteorological conditions. Although the mountain is small, the wooded slopes and the E–W direction of the ridge influence the passing air masses greatly [40]. The weather conditions during the three successive growing years are presented in Figure 2.
2.4. Essential Oil and Hydrolate Extraction
The small-scale distillation unit at the Institute of Field and Vegetable Crops Novi Sad, consists of a cylindrical distillation vessel with a conical bottom of stainless-steel pipe, which connects the vessel with a water-cooled condenser Florentine flask. The steam was produced externally, by a steam generator, and supplied the vessel with plant material via a pipe on the bottom. Our previous paper provides detailed information about capacity and conditions [41].
Briefly, 100 kg of fresh flowers of L. x intermedia cv. ‘Budrovka’ were placed in a distillation vessel, routed upwards through a plumbing system, and supplied with steam. After 20 min, the condensed vapours started to collect in the Florentine flask. After 2 h the distillation process was over. Accumulated essential oil floated on the water phase (hydrolate). The hydrolate was collected in sterile plastic bottles via filter paper, while essential oil was dried applying sodium sulphate and stored in amber glass bottles. The same extraction process was repeated three times.
2.5. Analysis of Volatile Compounds
The hydrolates before analysis were subjected to simultaneous distillation and extraction with dichloromethane via a Likens–Nickerson apparatus. Obtained essential oils and hydrolates samples were analysed by gas chromatography with flame ionization detector (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). GC-FID/MS analyses were performed according to Acimovic et al. [42] with some modifications of the split ratio. Injection volume was 1 μL, split ratio 10:1 for essential oils and 50:1 for hydrolates.
2.6. Statistical Analysis
A linear regression model was developed to predict the L. x intermedia cv. ‘Budrovka’ essential oil active compound content and hydrolate composition during three years according to temperature and precipitation data, and the appropriate regression coefficients were calculated using the linear formula: x = a + bT · T + bP · P, where x was EO active compound content or hydrolate content, a was the intercept, bT and bP were temperature and precipitation coefficients, while T and P were temperature and precipitation amounts, respectively.
The collected data were processed statistically using the software package STATISTICA 10.0.
3. Results
3.1. Fresh Herb Yield and Essential Oil Content
This variety (Vouch No 2-0987, Herbarium BUNS, Serbia), obtained from local nursery garden, is a shrub ranging up to 150 cm, with linear-lanceolate to spatulate leaves, often tomentose. Its inflorescence stalk is branched, and flowers show a corolla with bilateral symmetry and vary in colour from lilac-purple to white, blooming from late June to July. Flowering tops were collected at the full bloom stage. Harvest dates and yields are given in Table 2.
3.2. Chemical Composition of Essential Oil
The essential oil from L. x intermedia cultivated on Fruška Gora Mt. contains 56 compounds (Table 3). The most abundant compounds were: linalool (33.3–42.0%), 1,8-cineole (12.9–19.0%), borneol (8.1–16.1%), linalyl acetate (5.3–8.8%), and terpinene-4-ol (3.5–6.6%). All these compounds belong to oxygenated monoterpenes, which are the dominant compound class in essential oils.
Comparing rainfall among years (Figure 2), the growing season 2018/19 had significantly higher rainfall (694.2 mm) in comparison with the other two growing seasons (458.8 mm for 2019/20, and 416.9 mm for 2020/21). On the other hand, comparing average year temperatures for all three investigated seasons, the third growing season (2020/21) was slightly cooler (13.2 °C) in comparison to the other two (13.5 °C on average). Therefore, it could be said that temperature was in positive correlation with all essential oil compounds (temp. coeff. varied between 0.70 and 0.98), while precipitations were in negative correlation with a large number of essential oil compounds (prec. coeff. varied between −0.10 and −0.25), except linalool (0.59), 1,8-cineole (0.11), and borneol (0.01).
3.3. Chemical Composition of Hydrolate
There were 40E compounds identified in L. x intermedia cv. ‘Budrovka’ hydrolate (Table 4). The most abundant was linalool (21.9–32.1%), 1,8-cineole (12.7–26.2%), borneol (10.6–24.4%), terpinen-4-ol (6.4–12.2%), and cis- and trans-linalool oxides (1.4–11.5% and 1.3–10.9%, respectively).
During all three years oxygenated monoterpenes were the dominant class, with 92.6–96.0%. Similar to essential oils, differences in climate conditions during tree investigated years have not impacted hydrolate composition variations. In addition, the temperature was in positive correlation with all hydrolate compounds (temp. coeff. varied between 0.70 and 0.84), while precipitations were in negative correlation with a large number of essential oil compounds (prec. coeff. varied between −0.10 and −0.25), except linalool (0.33), 1,8-cineole (0.15), and borneol (0.10).
3.4. Correlation between Chemical Compounds of Essential Oil and Hydrolate
The correlation analysis was employed to examine the relations in hydrolate and essential oil compounds of L. x intermedia cv. ‘Budrovka’ samples from three growing years (2019, 2020, and 2021), and the results were displayed in Figure 3. It can be noticed from the figure that the darker blue colour of the squares, which indicates the two active compounds ‘content similarity, presents a more significant correlation linking observed active compounds. In contrast, the lighter tone indicates a particular dissimilarity active compound. Therefore, if the colour tone is lighter, consequently the correlation is lower. On the other hand, the red colour symbolizes a negative correlation between active compounds.
To thoroughly explain the structure of the research data that would provide a better perception of similarities and differences among diverse the L. x intermedia cv. ‘Budrovka’ samples from 2019, 2020, and 2021, PCA was used, and the results are presented in Figure 4. The first PC explained 43.46%, the second 19.10%, and the third 16.53% of the total variance within the experimental data. The separation between samples could be recognized from the PCA figures, where the samples from L. x intermedia cv. ‘Budrovka’ hydrolate composition during 2019, 2020, and 2021 are grouped on the right side of the graphic, while the samples from the L. x intermedia cv. ‘Budrovka’ essential oil composition during three growing years are grouped on the graphic’s left side. Table 5 represents the correlation matrix among active compounds content in hydrolate and essential oil of L. x intermedia cv. ‘Budrovka’ samples from 2019, 2020, and 2021.
4. Discussion
4.1. Essential Oil
Investigations conducted with L. latifolia showed that the environment significantly affected the essential oil quality, especially altitude [43]. Further, in the case of L. angustifolia, agronomical practices such as irrigation [44], variety [19], and substrate [45] significantly impact essential oil composition. In addition, investigations show that during the blooming period, linalool content was influenced by temperature; although, rainfalls remarkably decreased its production [46]. As it can be seen from Figure 4, our long-term experiment (three successive years) shows that on accumulation linalool and linalyl acetate precipitation, and the temperature are in positive relations, while in the case of linalyl acetate, the temperature is in positive (0.75), and precipitation in negative (−0.10) correlations.
The isolated essential oil of L. x intermedia cv. ‘Budrovka’ cultivated in Fruška gora Mt. did not comply with international standards requirements for the lavandin (ISO 8902), similar is with the same cultivar grown in Croatia, which contained linalool (57.1%), linalyl acetate (9.8%), and 1,8-cineole (8.5%) as dominant compounds [9]. One of the main criteria for Lavandula sp. essential oil quality is the ratio between linalool and its ester form, linalyl acetate. A ratio lower than one indicates the high quality of essential oil [47]. In this study, the linalool: linalyl acetate ratio ranged from 3.78 (in 2020) and 6.43 (in 2019) to 7.92 (in 2021). On average, this value was 6.04, while in Croatia, this ratio was 5.81 for the same cultivar [9].
Table 6 shows 28 samples of L. x intermedia essential oil from literature and average values from this study (total 29 accessions) with ten compounds from ISO standard specification. As can be seen, no sample satisfies all values required for essential oil quality. However, it is known that L. x intermedia is a cheaper hybrid, 6–7 times than L. angustifolia, and usually is mixed with them to obtain a higher quality of essential oil [16]. In addition, the accumulation of main compounds in Lavandula sp. plants are genetically influenced, i.e., by a key gene involved in controlling the production of linalyl acetate, camphor, 1,8-cineole, and borneol [48].
The primary source of variability in chemical composition and oil yield among the various populations of L. x intermedia are differences in environmental conditions [14].
According to 29 accessions of L. x intermedia from the literature, unrooted cluster tree (Figure 5) shows the presence of four chemotypes: (1) linalool + linalyl acetate, (2) linalool + 1,8-cineole, (3) linalool + camphor, and (4) 1,8-cineole + camphor. The first one, with dominant linalool and linalyl acetate, could be divided into three subgroups according to linalool:linalyl acetate ratio: (1) ratio ranged between 0.7 and 1.1—all three accessions from Turkey [4,49,50]; (2) ratio 1.8—one accession from Turkey [51]; and (3) ratio ranged from 1.8 to 5.5—five accessions from Italy [3,52,54], and one from Turkey [50]. In the second chemotype, the dominant compounds were linalool, and 1,8-cineole, with two subgroups: (1) 39.0–46.8% of linalool and 6.7–16.1% of 1,8-cineole [3,10,11,50], and this study; and (2) 55.2–65.8% of linalool and 3.0–10.0% of 1,8-cineole [9,10,54]. Linalool+camphor chemotype is present in Canada (five accessions, [19]) as well as with one sample from Turkey and Serbia [4,53]. Chemotype with dominant 1,8-cineole, and camphor is noted in Turkey (three accessions, [18]) and China [21].
4.2. Hydrolate
Lavandula sp. hydrolates haves a characteristic delicate lavender scent [28]. However, the chemical composition of hydrolates and essential oil, depends on plant part (herb or flower), postharvest processing (fresh or dry plant material), and isolation technique [55,56]. The chemical composition of different Lavandula sp. hydrolates found in literature and from the study is shown in Table 7. As can be seen, all Lavandula sp. hydrolates contain linalool (ranged between 7.7% and 55.6%). The content of other significant compounds (average content more than 1.5% according to 19 samples from literature, and average value from this study) are: linalool oxides (≤67.3%), 1,8-cineole (≤52.9%), camphor (≤19.6%), borneol (≤17.3%), α-terpineol (≤13.0%), terpinen-4-ol (≤9.4%), and geraniol (≤5.0%). The most abundant group of compounds are oxygenated monoterpenes, mainly monoterpene alcohols [28]. The hydrosols were not found to contain linalyl acetate or sesquiterpenes, which are present in the essential oil [28,49].
Moroccan hydrolate of L. officinalis contained linalool as the dominant compound, followed by camphor, 1,8-cineole, α-terpineol, and borneol [57]. The main component of the volatile fraction of L. angustifolia from Poland was linalool, followed by α-terpineol, borneol, and geraniol [55], while in another study from Poland, it was linalool, followed by α-terpineol and terpinen-4-ol [28,58]. Hydrolate obtained from L. angustifolia buds from Croatia had the most significant proportion of 1,8-cineole, linalool oxide, and linalool [56]. Turkish L. x intermedia hydrolate had linalool, borneol, and camphor as the main compounds [49], while Italian L. intermedia samples contained linalool, 1,8-cineole, and camphor in different proportions [29,30,31]. Serbian L. x intermedia hydrolate had a high percentage of linalool oxides. A higher abundance of monoterpene alcohols oxides (cis- and trans-linalool) in hydrolate compared to the corresponding oil is probably due to their better solubility in water [53].
According to the unrooted cluster tree (Figure 6), it could be said that in Lavandula sp. hydrolate linalool dominated in almost all samples (in different proportions, from 25.7% to 55.6%), while in one sample, the dominant compound was linalool oxide [53], and in three it was 1,8-cineole [29,56]. Moreover, there is no difference between the chemical compositions of L. angustifolia and L. x intermedia hydrolates.
5. Conclusions
This research has proved that Fruška Gora (Serbia) has good agro-ecological conditions for cultivating Lavandula sp. and producing acceptable quality essential oil and hydrolate. Growing this species in this region could be recommended as Fruška Gora is a protected area suitable for organic farming. Since Lavandula sp. is a pollen and nectar-producing plant, it can positively affect the ecosystem’s biodiversity. Honeybees are the most common visitors to Lavandula sp. fields and produce lavender honey. This type of unifloral honey has a high commercial value, which further supports the cultivation of this species. Tourism on Fruška Gora is quite developed due to many Orthodox monasteries, vineyards, and small wineries. Therefore, lavender fields could improve touristic content in this region following examples of other countries.
Author Contributions
Conceptualization, M.A. and B.L.; methodology, J.S.J.; software, L.P.; validation, M.C., M.P. and M.T.; formal analysis, J.S.J.; investigation, B.L.; resources, M.A.; data curation, L.P.; writing—original draft preparation, M.A.; writing—review and editing, V.T.; visualization, M.P.; supervision, B.L.; project administration, M.A.; funding acquisition, M.A., B.L. and M.P. All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia, grant number 451-03-68/2022-14/200032, 451-03-68/2022-14/200134, 451-03-68/2022-14/200168, 451-03-68/2022-14/ 200051, 337-00-21/2020-09/40.
.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Not applicable.
Acknowledgments
The authors are thankful to Branko Švonja for providing plant material, field photography, and initiating this investigation.
Conflicts of Interest
The authors declare no conflict of interest.
References
- Lesage-Meessen, L.; Bou, M.; Sigoillot, J.C.; Faulds, C.B.; Lomascolo, A. Essential oils and distilled straws of lavender and lavandin: A review of current use and potential application in white biotechnology. Appl. Microbiol. Biotechnol. 2015, 99, 3375–3385. [Google Scholar] [CrossRef]
- Szekely-Varga, Z.; Hitter, T.; Cantor, M. The healing power and the uses in landscape design of lavender (Lavandula angustifolia L.). Hop Med. Plants 2017, 25, 47–55. [Google Scholar]
- Pistelli, L.; Najar, B.; Giovanelli, S.; Lorenzini, L.; Tavarini, S.; Angelini, L.G. Agronomic and phytochemical evaluation of lavandin and lavender cultivars cultivated in the Tyrrhenian area of Tuscany (Italy). Ind. Crops Prod. 2017, 109, 37–44. [Google Scholar] [CrossRef]
- Kivrak, S. Essential oil composition and antioxidant activities of eight cultivars of lavender and lavandin from western Anatolia. Ind. Crops Prod. 2018, 117, 88–96. [Google Scholar] [CrossRef]
- Florina, G.E.; Camelia, M.; Teodor, G.V. Lavender-perfume and business in Romania. Agric. Res. Technol. 2019, 19, 140–144. [Google Scholar] [CrossRef]
- Detar, E.; Zambori-Nemeth, E.; Gosztola, B.; Harmath, A.; Ladanyi, M.; Pluhar, Z. Ontogenesis and harvest time are crucial for high quality lavender—Role of the flower development in essential oil properties. Ind. Crops Prod. 2021, 163, 113334. [Google Scholar] [CrossRef]
- Pecanha, D.A.; Freitas, M.S.M.; Vieira, M.E.; Cunha, J.M.; de Jesus, A.C. Phosphorus fertilization affects growth, essential oil yield and quality of true lavender in Brazil. Ind. Crops Prod. 2021, 170, 113803. [Google Scholar] [CrossRef]
- Stanković, I.; Vrandečić, K.; Ćosić, J.; Milojević, K.; Bulajić, A.; Krstić, B. The spreading of Alfalfa mosaic virus in lavandin in Croatia. Pestic. Phytomed. 2014, 29, 115–122. [Google Scholar] [CrossRef]
- Blažeković, B.; Yang, W.; Wang, Y.; Li, C.; Kindl, M.; Pepeljnjak, S.; Vladimir-Knežević, S. Chemical composition, antimicrobial and antioxidant activities of essential oils of Lavandula × intermedia ‘Budrovka’ and L. angustifolia cultivated in Croatia. Ind. Crops Prod. 2018, 123, 173–182. [Google Scholar] [CrossRef]
- Detar, E.; Zamborine Nemeth, E.; Gosztola, B.; Demjan, I.; Pluhar, Z. Effect of variety and growth year on the essential oil properties of lavender (Lavandula angustifolia Mill.) and lavandin (Lavandula × intermedia Emeric ex Liosel.). Biochem. Syst. Ecol. 2020, 90, 104020. [Google Scholar] [CrossRef]
- Karapandzova, M.; Cvetkovikj, I.; Stefkov, G.; Stoimenov, V.; Crvenov, M.; Kulevanova, S. The influence of duration of the distillation of fresh and dried flowers on the essential oil composition of lavandin cultivated in Republic Macedonia. Maced. Pharm. Bull. 2012, 58, 31–38. [Google Scholar] [CrossRef]
- Jianu, C.; Pop, G.; Gruia, A.T.; Horhat, F.G. Chemical composition and antimicrobial activity of essential oils of lavender (Lavandula angustifolia) and lavandin (Lavandula × intermedia) grown in Western Romania. Int. J. Agric. Biol. 2013, 15, 772–776. [Google Scholar]
- Prusinowska, R.; Śmigielsk, K.B. Composition, biological properties and therapeutic effects of lavender (Lavandula angustifolia L.). A review. Herba Pol. 2014, 60, 56–66. [Google Scholar] [CrossRef] [Green Version]
- Bajalan, I.; Pirbalouti, A.G. Variation in chemical composition of essential oil of populations of Lavandula × intermedia collected from Western Iran. Ind. Crops Prod. 2015, 69, 344–347. [Google Scholar] [CrossRef]
- Pokajewicz, K.; Bialon, M.; Svydenko, L.; Fedin, R.; Hudz, N. Chemical composition of the essential oil of the new cultivars of Lavandula angustifolia Mill. bread in Ukraine. Molecules 2021, 26, 5681. [Google Scholar] [CrossRef] [PubMed]
- Marincas, O.; Feher, I. A new cost-effective approach for lavender essential oils quality assessment. Ind. Crops Prod. 2018, 125, 241–247. [Google Scholar] [CrossRef]
- Giray, F.H. An analysis of world lavender oil markets and lessons for Turkey. J. Essent. Oil-Bear. Plants 2018, 21, 1612–1623. [Google Scholar] [CrossRef]
- Ozel, A. Determining leaf yield, some plant characters and leaf essential oil components of different cultivars of lavender and lavandin (Lavandula spp.) on the Harran plain ecological conditions. Appl. Ecol. Environ. Res. 2019, 17, 14087–14094. [Google Scholar] [CrossRef]
- Lane, A.W.; Mahmoud, S.S. Composition of essential oil from Lavandula angustifolia and L. intermedia varieties grown in British Columbia, Canada. Nat. Prod. Commun. 2008, 3, 1361–1366. [Google Scholar] [CrossRef] [Green Version]
- Stanojević, L.; Stanković, M.; Cakić, M.; Nikolić, V.; Nikolić, L.; Ilić, D.; Radulović, N. The effect of hydrodistillation techniques on yield, kinetics, composition and antimicrobial activity of essential oils from flowers of Lavandula officinalis L. Hem. Ind. 2011, 65, 455–463. [Google Scholar] [CrossRef]
- Liao, Z.; Huang, Q.; Cheng, Q.; Khan, S.; Yu, X. Seasonal variation in chemical composition of essential oils extracted from lavandin flowers in the Yun-Gui plateau of China. Molecules 2021, 26, 5639. [Google Scholar] [CrossRef]
- Bilia, A.R.; Guccione, C.; Isacchi, B.; Righeschi, C.; Firenzuoli, F.; Bergonzi, M.C. Essential oils loaded in nanosystems: A developing strategy for a successful therapeutic approach. J. Evid.-Based Complement. Altern. Med. 2014, 2014, 651593. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mariod, A.A. Chapter 13—Effect of essential oils on organoleptic (smell, taste, and texture) properties of food. In Essential Oils in Food Preservation, Flavor and Safety; Preedy, V.R., Ed.; Academic Press: Cambridge, MA, USA, 2016; pp. 131–137. [Google Scholar] [CrossRef]
- Aćimović, M.; Tešević, V.; Smiljanić, K.; Cvetković, M.; Stanković, J.; Kiprovski, B.; Sikora, V. Hydrolates—By-products of essential oil distillation: Chemical composition, biological activity and potential uses. Adv. Technol. 2020, 9, 54–70. [Google Scholar] [CrossRef]
- Jakubczyk, K.; Tuchowska, A.; Janda-Milczarek, K. Plant hydrolates—Antioxidant properties, chemical composition and potential applications. Biomed. Pharmacother. 2021, 142, 112033. [Google Scholar] [CrossRef]
- Pino-Otin, M.R.; Navarro, J.; Val, J.; Roig, F.; Mainar, A.M.; Ballestero, D. Spanish Satureja montana L. hydrolate: Ecotoxicological study in soil and water non-target organisms. Ind. Crops Prod. 2022, 178, 114553. [Google Scholar] [CrossRef]
- Ferraz, C.A.; Sousa, A.C.; Caramelo, D.; Delgado, F.; de Oliveira, A.P.; Pastorinho, R. Chemical profile and eco-safety evaluation of essential oils and hydrolates from Cistus ladanifer, Helichrysum italicum, Ocimum basilicum and Thymbra capitata. Ind. Crops Prod. 2022, 175, 114232. [Google Scholar] [CrossRef]
- Prusinowska, R.; Smigielski, K.B. Hydrosols from lavender (Lavandula angustifoia)—Determination of the chemical composition using Dispersive Liquid-Liquid Microextraction (DLLME). J. Essent. Oil-Bear. Plants. 2015, 18, 519–528. [Google Scholar] [CrossRef]
- Garzoli, S.; Petralito, S.; Ovidi, E.; Turchetti, G.; Masi, V.L.; Tiezzi, A.; Trilli, J.; Cesa, S.; Casadei, M.A.; Giacomello, P.; et al. Lavandula × intermedia essential oil and hydrolate: Evaluation of chemical composition and antibacterial activity before and after formulation in nanoemulsion. Ind. Crops Prod. 2022, 145, 1126068. [Google Scholar] [CrossRef]
- Politi, M.; Menghini, L.; Conti, B.; Bedini, S.; Farina, P.; Cioni, P.L.; Braca, A.; De Leo, M. Reconsidering hydrosols as main products of aromatic plants manufactory: The lavandin (Lavandula × intermedia) case study in Tuscany. Molecules 2020, 25, 2225. [Google Scholar] [CrossRef] [PubMed]
- Garzoli, S.; Masci, V.L.; Franceschi, S.; Tiezzi, A.; Giacomello, P.; Ovidi, E. Headspace/GC-MS analysis and investigation of antibacterial, antioxidant and cytotoxic activity of essential oils and hydrolates from Rosmarinus officinalis L. and Lavandula angustifolia Miller. Foods 2021, 10, 1768. [Google Scholar] [CrossRef] [PubMed]
- Vujičić, M.D.; Vasiljević, D.A.; Markovi, S.B.; Hose, T.A.; Lukić, T.; Hadžić, O.; Janićević, S. Preliminary geosite assessment model (GAM) and its application on Fruška Gora Mountain, potential geotourism destination of Serbia. Acta Geogr. Slov. 2011, 51, 361–376. [Google Scholar] [CrossRef]
- Renaud, E.; Charles, D. Essential oil quality and composition from 10 cultivars of organically grown lavender and lavandin. J. Essent. Oil Res. 2001, 13, 269–273. [Google Scholar] [CrossRef]
- Giannoulis, K.D.; Evangelopoulos, V.; Gougoilias, N.; Wogiatzi, E. Lavender organic cultivation yield and essential oil can be improved by using bio-stimulants. Acta Agric. Scand. Sect. B 2020, 70, 648–656. [Google Scholar] [CrossRef]
- Seidler-Lozykowska, K.; Mordalski, R.; Kucharski, W.; Kedzia, B.; Bocianowski, J. Yielding and quality of lavender flowers (Lavandula angustifolia Mill.) from organic cultivation. Acta Sci. Pol. Hortorum Cultus 2014, 13, 173–183. [Google Scholar]
- Salehi, B.; Mnayer, D.; Özçelik, B.; Altin, G.; Kasapoğlu, K.N.; Daskaya-Dikmen, C.; Sharifi-Rad, M.; Selamoglu, Z.; Acharya, K.; Sen, S.; et al. Plants of the genus Lavandula: From farm to pharmacy. Nat. Prod. Commun. 2018, 13, 1385–1402. [Google Scholar] [CrossRef] [Green Version]
- Blažeković, B.; Stanic, G.; Pepeljnjak, S.; Vladimir-Knezevic, S. In vitro antibacterial and antifungal activity of Lavandula × intermedia Emeric ex Loisel. ‘Budrovka’. Molecules 2011, 16, 4241–4253. [Google Scholar] [CrossRef]
- Vrandečić, K.; Ćosić, J.; Jurković, D.; Ilić, J. Bolesti lavande u istočnom delu Hrvatske. Glas. Biljn. Zaštite 2013, 5, 391–396. (In Croatian) [Google Scholar]
- Aćimović, M.; Pezo, L.; Zeremski, T.; Lončar, B.; Marjanović Jeromela, A.; Stanković Jeremić, J.; Cvetković, M.; Sikora, V.; Ignjatov, M. Weather conditions influence on hyssop essential oil quality. Processes 2021, 9, 1152. [Google Scholar] [CrossRef]
- Mészáros, M.; Markovlc, S.B.; Mijovkć, D.; Jovanovlć, M. Physical geographic characteristics and geo-heritage of Fruska gora mountain (Vojvodina, Serbia). Acta Geogr. Szeged. 2004, 38, 148–157. [Google Scholar]
- Aćimović, M.; Cvetković, M.; Stanković Jeremić, J.; Pezo, L.; Varga, A.; Čabarkapa, I.; Kiprovski, B. Biological activity and profiling of Salvia sclarea essential oil obtained by steam and hydrodistillation extraction methods via chemometrics tools. Flavour Fragr. J. 2022, 37, 20–32. [Google Scholar] [CrossRef]
- Aćimović, M.; Ivanović, S.; Simić, K.; Pezo, L.; Zeremski, T.; Ovuka, J.; Sikora, V. Chemical Characterization of Marrubium vulgare Volatiles from Serbia. Plants 2021, 10, 600. [Google Scholar] [CrossRef] [PubMed]
- Fernández-Sestelo, M.; Carrillo, J.M. Environmental effects on yield and composition of essential oil in wild populations of spike lavender (Lavandula latifolia Medik.). Agriculture 2020, 10, 626. [Google Scholar] [CrossRef]
- Salata, A.; Buczkowska, H.; Nurzynska-Wierdak, R. Yield, essential oil content, and quality performance of Lavandula angustifolia leaves, as affected by supplementary irrigation and drying methods. Agriculture 2020, 10, 590. [Google Scholar] [CrossRef]
- Najar, B.; Demasi, S.; Caser, M.; Gaino, W.; Cioni, P.L.; Pistelli, L.; Scario, V. Cultivation substrate composition influences morphology, volatilome and essential oil of Lavandula angustifolia Mill. Agronomy 2019, 9, 411. [Google Scholar] [CrossRef] [Green Version]
- Hassiotis, C.N.; Ntana, F.; Lazari, D.M.; Poulios, S.; Vlachonasios, K.E. Environmental and developmental factors affect essential oil production and quality of Lavandula angustifolia during flowering period. Ind. Crops Prod. 2014, 62, 359–366. [Google Scholar] [CrossRef]
- Porter, N.G.; Shaw, M.L.; Hurndell, L.C. Preliminary studies of lavender as an essential oil crop for New Zealand. N. Z. J. Agric. Res. 1982, 25, 389–394. [Google Scholar] [CrossRef]
- Desautels, A.; Biswas, K.; Lane, A.; Boeckelmann, A.; Mahmoud, S.S. Suppression of linalool acetate production in Lavandula × intermedia. Nat. Prod. Commun. 2009, 4, 1533–1536. [Google Scholar] [CrossRef] [Green Version]
- Baydar, H.; Kineci, S. Scent composition of essential oil, concrete, absolute and hydrosol from lavandin (Lavandula × intermedia Emeric ex Loisel.). J. Essent. Oil-Bear. Plants. 2009, 12, 131–136. [Google Scholar] [CrossRef]
- Kara, N.; Baydar, H. Determination of lavender and lavandin cultivars (Lavandula sp.) containing high quality essential oil in Isparta, Turkey. Turk. J. Field Crop. 2013, 18, 58–65. [Google Scholar]
- Karaman, R.; Erbas, S.; Baydar, H.; Kaya, M. Allelopathic effect of lavandin (Lavandula × intermedia Emeric ex Loilsel. Var Super A) oil on germination and seedling development of some weed and field crops. Harran Tarım ve Gıda Bilimleri Derg. 2014, 18, 35–41. [Google Scholar]
- Garzoli, S.; Turchetti, G.; Giacomello, P.; Tiezzi, A.; Masci, V.L.; Ovidi, E. Liquid and vapour phase of lavandin (Lavandula × intermedia) essential oil: Chemical composition and antimicrobial activity. Molecules 2019, 24, 2701. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Pljevljakušić, D.; Drinić, Z. GC/MS chemical analysis of lavandin (Lavandula × intermedia) hydrolat: Sucessive extraction fraction. Lek. Sirovine 2020, 40, 33–39. [Google Scholar] [CrossRef]
- D’Addabbo, T.; Laquale, S.; Argentieri, M.P.; Bellardi, M.G.; Avato, P. Neatocidal activity of essential oil from lavandin (Lavandula × intermedia Emeric ex Loisel.) as related to chemical profile. Molecules 2021, 26, 6448. [Google Scholar] [CrossRef] [PubMed]
- Kunicka-Styczynska, A.; Smigielski, K.; Prusinowska, R.; Rajkowska, K.; Kusmider, B.; Sikora, M. Preservative activity of lavender hydrosols in moisturizing body gels. Lett. Appl. Microbiol. 2014, 60, 27–32. [Google Scholar] [CrossRef]
- Silha, D.; Švarcova, K.; Bajer, T.; Kralovec, K.; Tesarova, E.; Mouckova, M.; Bajerova, P. Chemical composition of natural hydrolates and their antimicrobial activity on Arcobacter-like cells in comparison with other microorganisms. Molecules 2020, 25, 5654. [Google Scholar] [CrossRef]
- Azza, S.; Lyoussi, B.; Miguel, M. Antioxidant activity of some Morrocan hydrosols. J. Med. Plants Res. 2011, 5, 6688–6696. [Google Scholar] [CrossRef]
- Prusinowska, R.; Smigielski, K.; Kunicka-Styczynska, A. Hydrolates from lavender (Lavandula angustifolia)—Chemical composition, antimicrobial and antioxidant properties. PhD Interdiscip. J. 2013, 3, 33–39. [Google Scholar]
Figure 1.
Field location and lavender field in full blooming with honeybees.
Figure 2.
The weather conditions during the three successive growing seasons.
Figure 3.
Correlation between active compounds content in hydrolate and essential oil of L. x intermedia cv. ‘Budrovka’ samples from 2019, 2020, and 2021 (active compounds codes are listed in Table 3 and Table 4).
Figure 4.
The PCA biplot diagram depicting the relationships among hydrolate and essential oil compounds of L. x intermedia cv. ‘Budrovka’ samples from three growing years: (a) projection of variables and cases in the PC1 and PC2 plane, (b) projection of variables and cases in PC1 and PC3 plane (active compounds codes and samples codes are listed in Table 3 and Table 5).
Figure 4.
The PCA biplot diagram depicting the relationships among hydrolate and essential oil compounds of L. x intermedia cv. ‘Budrovka’ samples from three growing years: (a) projection of variables and cases in the PC1 and PC2 plane, (b) projection of variables and cases in PC1 and PC3 plane (active compounds codes and samples codes are listed in Table 3 and Table 5).
Figure 5.
Unrooted cluster tree for chemical composition of L. x intermedia essential oil (samples are coded according to Table 6.
Figure 5.
Unrooted cluster tree for chemical composition of L. x intermedia essential oil (samples are coded according to Table 6.
Figure 6.
Unrooted cluster tree for chemical composition of L. x intermedia hydrolate according to literature and this study (samples are coded according to Table 7).
Figure 6.
Unrooted cluster tree for chemical composition of L. x intermedia hydrolate according to literature and this study (samples are coded according to Table 7).
Table 1.
Basic chemical characteristics of the soil.
| pH | CaCO3 | Humus | Total Nitrogen | P2O5 | K2O | ||
|---|---|---|---|---|---|---|---|
| 1MKCl | H2O | (%) | (mg·100 g−1 Soil) | ||||
| Hill | 7.13 | 8.22 | 11.27 | 1.03 | 0.051 | 3.6 | 8.0 |
| Middle | 7.19 | 8.34 | 8.10 | 0.56 | 0.028 | 7.4 | 8.0 |
| Valley | 7.41 | 8.31 | 7.89 | 0.96 | 0.048 | 7.4 | 8.0 |
Table 2.
Date of harvest, fresh herb yield and essential oil content for L. x intermedia cv. ‘Budrovka’ cultivated in Fruška gora Mt. during 2019–2021.
Table 2.
Date of harvest, fresh herb yield and essential oil content for L. x intermedia cv. ‘Budrovka’ cultivated in Fruška gora Mt. during 2019–2021.
| 2019 | 2020 | 2021 | |
|---|---|---|---|
| Date of harvest | 11th–17th July | 27th June–2nd July | 9th–15th July |
| Fresh herb yield (kg/ha) | 4.840 | 5.040 | 5.670 |
| Essential oil content (%) | 1.26 | 1.19 | 1.03 |
Table 3.
L. x intermedia cv. ‘Budrovka’ essential oil composition during three growing years and related multiple correlation coefficients with the observed temperature and precipitation amounts.
Table 3.
L. x intermedia cv. ‘Budrovka’ essential oil composition during three growing years and related multiple correlation coefficients with the observed temperature and precipitation amounts.
| No | Compound | RI | 2019 | 2020 | 2021 | Temp. Coeff. | Prec. Coeff. |
|---|---|---|---|---|---|---|---|
| EO19 | EO20 | EO21 | |||||
| 6 | n-hexanol O | 861 | 0.2 | - | - | 0.70 | −0.25 |
| 7 | α-thujene MT | 924 | 0.1 | 0.1 | 0.1 | 0.70 | −0.25 |
| 8 | α-pinene MT | 931 | 0.9 | 0.6 | 1.4 | 0.71 | −0.23 |
| 9 | camphene MT | 945 | 0.4 | 0.7 | 0.5 | 0.71 | −0.24 |
| 12 | sabinene MT | 970 | 0.3 | 0.1 | 0.4 | 0.70 | −0.24 |
| 14 | β-pinene MT | 974 | 1.6 | 0.9 | 2.1 | 0.71 | −0.21 |
| 15 | 3-octanone O | 984 | 0.1 | - | - | 0.70 | −0.25 |
| 16 | dehydro-1,8-cineole OMT | 989 | - | 0.1 | - | 0.70 | −0.25 |
| 17 | myrcene MT | 990 | 0.5 | 0.1 | 0.7 | 0.70 | −0.24 |
| 18 | δ-3-carene MT | 1008 | 0.3 | 0.1 | 0.4 | 0.70 | −0.24 |
| 19 | hexyl acetate O | 1010 | 0.1 | - | - | 0.70 | −0.25 |
| 20 | α-terpinene MT | 1013 | - | - | 0.1 | 0.70 | −0.25 |
| 22 | p-cymene MT | 1022 | 0.4 | 0.8 | 0.3 | 0.71 | −0.24 |
| 23 | limonene MT | 1025 | 1.1 | 0.7 | 1.1 | 0.71 | −0.23 |
| 24 | 1,8-cineole OMT | 1028 | 12.9 | 16.4 | 19.0 | 0.82 | 0.11 |
| 25 | cis-β-ocimene MT | 1033 | 1.9 | - | 3.6 | 0.72 | −0.21 |
| 27 | trans-β-ocimene MT | 1044 | 0.2 | - | 0.4 | 0.70 | −0.24 |
| 28 | γ-terpinene MT | 1055 | 0.1 | - | 0.2 | 0.70 | −0.25 |
| 29 | cis-sabinene hydrate (IPP vs OH) OMT | 1063 | 0.2 | 0.2 | - | 0.70 | −0.25 |
| 30 | cis-linalool oxide (furanoid) OMT | 1069 | 0.1 | 2.4 | - | 0.71 | −0.23 |
| 32 | trans-linalool oxide (furanoid) OMT | 1085 | - | 2.1 | - | 0.71 | −0.23 |
| 33 | terpinolene MT | 1086 | 0.2 | - | 0.3 | 0.70 | −0.24 |
| 34 | linalool OMT | 1101 | 41.8 | 33.3 | 42.0 | 0.98 | 0.59 |
| 36 | allo-ocimene MT | 1126 | 0.3 | - | 0.6 | 0.70 | −0.24 |
| 38 | trans-pinocarveol OMT | 1134 | - | 0.3 | - | 0.70 | −0.25 |
| 40 | camphor OMT | 1141 | 3.7 | 4.6 | 3.9 | 0.73 | −0.16 |
| 41 | hexyl isobutanoate O | 1145 | 0.1 | 0.1 | - | 0.70 | −0.25 |
| 43 | borneol OMT | 1164 | 11.4 | 16.1 | 8.1 | 0.79 | 0.01 |
| 45 | trans-linalool oxide (pyranoid) OMT | 1168 | - | 0.2 | - | 0.70 | −0.25 |
| 46 | terpinen-4-ol OMT | 1175 | 6.6 | 3.5 | 5.3 | 0.74 | −0.14 |
| 48 | cryptone O | 1180 | - | 0.3 | - | 0.70 | −0.25 |
| 49 | α-terpineol OMT | 1188 | 0.8 | 0.5 | - | 0.70 | −0.24 |
| 50 | hexyl butanoate O | 1189 | 0.4 | 0.4 | 0.5 | 0.70 | −0.24 |
| 51 | myrtenal OMT | 1190 | - | 0.2 | - | 0.70 | −0.25 |
| 55 | hexyl 2-methyl butanoate O | 1234 | 0.2 | 0.3 | 0.1 | 0.70 | −0.24 |
| 56 | cumin aldehyde O | 1236 | - | 0.1 | - | 0.70 | −0.25 |
| 57 | carvone OMT | 1238 | - | 0.1 | - | 0.70 | −0.25 |
| 58 | hexyl isovalerate O | 1239 | 0.1 | - | - | 0.70 | −0.25 |
| 61 | linalyl acetate OMT | 1254 | 6.5 | 8.8 | 5.3 | 0.75 | −0.10 |
| 63 | bornyl acetate OMT | 1284 | 0.1 | 0.2 | - | 0.70 | −0.25 |
| 64 | lavandulyl acetate OMT | 1289 | 0.6 | 1.2 | 0.3 | 0.71 | −0.23 |
| 65 | neryl acetate OMT | 1363 | 0.1 | - | - | 0.70 | −0.25 |
| 66 | daucene ST | 1377 | 0.1 | - | - | 0.70 | −0.25 |
| 67 | geranyl acetate OMT | 1382 | 0.2 | - | - | 0.70 | −0.25 |
| 68 | hexyl hexanoate O | 1384 | 0.2 | 0.2 | - | 0.70 | −0.25 |
| 69 | 7-epi-sesquithujene ST | 1388 | 0.1 | - | - | 0.70 | −0.25 |
| 70 | sesquithujene ST | 1403 | 0.1 | - | - | 0.70 | −0.25 |
| 71 | α-santalene ST | 1414 | - | 0.3 | - | 0.70 | −0.25 |
| 72 | trans-caryophyllene ST | 1417 | 0.9 | - | 0.6 | 0.70 | −0.24 |
| 73 | trans-α-bergamotene ST | 1433 | 0.1 | - | - | 0.70 | −0.25 |
| 74 | trans-β-farnesene ST | 1455 | 2.0 | 0.3 | 1.1 | 0.71 | −0.23 |
| 75 | germacrene D ST | 1480 | 0.3 | - | 0.1 | 0.70 | −0.25 |
| 76 | lavandulyl isovalerate OST | 1508 | 0.3 | 0.4 | - | 0.70 | −0.24 |
| 77 | γ-cadinene ST | 1513 | tr | 0.1 | - | 0.70 | −0.25 |
| 79 | caryophyllene oxide OST | 1580 | 0.2 | 0.8 | - | 0.70 | −0.24 |
| 80 | epi-α-bisabolol OST | 1682 | 0.1 | - | - | 0.70 | −0.25 |
| Monoterpene hydrocarbons (MT) | 8.3 | 4.1 | 12.2 | ||||
| Oxygenated monoterpenes (OMT) | 85.0 | 90.2 | 83.9 | ||||
| Sesquiterpene hydrocarbons (ST) | 3.6 | 0.7 | 1.8 | ||||
| Oxygenated sesquiterpens (OST) | 0.6 | 1.2 | - | ||||
| Other (O) | 1.4 | 1.4 | 0.6 | ||||
| Total identified | 98.9 | 97.6 | 98.5 | ||||
RI—Retention index (relative to C8–C36 n-alkanes on HP-5MSI column).
Table 4.
L. x intermedia cv. ‘Budrovka’ hydrolate composition during three growing years and related multiple correlation coefficients with the observed temperature and precipitation amounts.
Table 4.
L. x intermedia cv. ‘Budrovka’ hydrolate composition during three growing years and related multiple correlation coefficients with the observed temperature and precipitation amounts.
| No | Compound | RI | 2019 | 2020 | 2021 | Temp. Coeff. | Prec. Coeff. |
|---|---|---|---|---|---|---|---|
| EO19 | EO20 | EO21 | |||||
| 1 | 3-methyl-2-butenal O | 772 | 0.1 | - | 0.4 | 0.70 | −0.24 |
| 2 | hexanal O | 798 | - | - | 0.2 | 0.70 | −0.25 |
| 3 | 2,2-dimethyl-3(2H)-furanone O | 829 | - | - | 0.1 | 0.70 | −0.25 |
| 4 | furfural O | 832 | - | - | 0.1 | 0.70 | −0.25 |
| 5 | cis-3-hexenol O | 847 | - | - | 0.3 | 0.70 | −0.25 |
| 6 | n-hexanol O | 858 | 1.0 | 0.6 | 2.2 | 0.71 | −0.22 |
| 10 | 4-methyl pent-2-enolide (impure) O | 949 | 0.1 | - | 0.3 | 0.70 | −0.25 |
| 11 | 2-ethenyltetrahydro-2,6,6-trimethyl-2H-pyran O | 968 | 0.1 | 0.1 | - | 0.70 | −0.25 |
| 13 | 1-octen-3-ol O | 974 | 0.9 | 0.6 | 0.9 | 0.71 | −0.23 |
| 15 | 3-octanone O | 982 | - | - | 0.1 | 0.70 | −0.25 |
| 16 | dehydro-1,8-cineole OMT | 990 | tr | - | 0.1 | 0.70 | −0.25 |
| 21 | 1,4-cineole OMT | 1014 | 0.1 | - | - | 0.70 | −0.25 |
| 24 | 1,8-cineole OMT | 1030 | 12.7 | 14.4 | 26.2 | 0.84 | 0.15 |
| 26 | lavender lactone O | 1036 | - | 0.2 | 0.1 | 0.70 | −0.25 |
| 30 | cis-linalool oxide (furanoid) OMT | 1071 | 1.4 | 11.5 | 2.7 | 0.74 | −0.13 |
| 31 | camphenilone NOMT | 1080 | - | - | 0.1 | 0.70 | −0.25 |
| 32 | trans-linalool oxide (furanoid) OMT | 1088 | 1.3 | 10.9 | 2.4 | 0.74 | −0.14 |
| 34 | linalool OMT | 1102 | 26.0 | 21.9 | 32.1 | 0.90 | 0.33 |
| 35 | hotrienol OMT | 1102 | - | 1.0 | - | 0.70 | −0.24 |
| 37 | nopinone NOMT | 1136 | 0.2 | 0.2 | 0.2 | 0.70 | −0.24 |
| 38 | trans-pinocarveol OMT | 1134 | - | 0.2 | 0.1 | 0.70 | −0.25 |
| 39 | trans-sabinol (trans for OH vs. IPP) OMT | 1136 | 0.1 | - | - | 0.70 | −0.25 |
| 40 | camphor OMT | 1143 | 7.1 | 4.5 | 6.3 | 0.74 | −0.12 |
| 42 | neoiso-3-thujanol OMT | 1146 | - | 0.2 | - | 0.70 | −0.25 |
| 43 | borneol OMT | 1166 | 24.4 | 16.8 | 10.6 | 0.81 | 0.10 |
| 44 | cis-linalool oxide (pyanoid) OMT | 1168 | 0.6 | 1.0 | 0.5 | 0.71 | −0.23 |
| 45 | trans-linalool oxide (pyanoid) OMT | 1173 | 0.4 | 0.8 | 0.4 | 0.71 | −0.24 |
| 46 | terpinen-4-ol OMT | 1177 | 12.2 | 6.4 | 9.7 | 0.77 | −0.05 |
| 47 | p-cymen-8-ol O | 1179 | - | 0.2 | 0.2 | 0.70 | −0.24 |
| 48 | cryptone O | 1180 | - | 1.0 | 0.3 | 0.71 | −0.24 |
| 49 | α-terpineol OMT | 1190 | 6.0 | 4.5 | 1.4 | 0.73 | −0.17 |
| 51 | myrtenol OMT | 1191 | - | 0.1 | - | 0.70 | −0.25 |
| 52 | verbenone OMT | 1208 | 0.2 | 0.3 | 0.1 | 0.70 | −0.24 |
| 53 | trans-carveol OMT | 1216 | - | 0.1 | - | 0.70 | −0.25 |
| 54 | nerol OMT | 1222 | - | 0.3 | - | 0.70 | −0.25 |
| 57 | carvone OMT | 1243 | 0.1 | 0.1 | - | 0.70 | −0.25 |
| 60 | geraniol OMT | 1248 | - | 0.9 | - | 0.70 | −0.24 |
| 61 | linalyl acetate OMT | 1248 | - | - | 0.2 | 0.70 | −0.25 |
| 64 | lavandulyl acetate OMT | 1286 | - | 0.1 | - | 0.70 | −0.25 |
| 78 | cis-nerolidol OST | 1526 | - | - | 0.2 | 0.70 | −0.25 |
| Nor oxygenated monoterpenes (NOMT) | 0.2 | 0.2 | 0.3 | ||||
| Oxygenated monoterpenes (OMT) | 92.6 | 96.0 | 92.8 | ||||
| Sesquiterpene hydrocarbons (ST) | - | - | - | ||||
| Oxygenated sesquiterpens (OST) | - | - | 0.2 | ||||
| Other (O) | 2.2 | 2.7 | 5.2 | ||||
| Total identified | 95.0 | 98.9 | 98.5 | ||||
RI—Retention index (relative to C8–C36 n-alkanes on HP-5MSI column).
Table 5.
Correlation matrix between active compounds content in hydrolate and essential oil of L. x intermedia cv. ‘Budrovka’ samples from 2019, 2020, and 2021.
Table 5.
Correlation matrix between active compounds content in hydrolate and essential oil of L. x intermedia cv. ‘Budrovka’ samples from 2019, 2020, and 2021.
| EO20 | EO21 | H19 | H20 | H21 | |
|---|---|---|---|---|---|
| EO19 | 0.967 | 0.987 | 0.855 | 0.795 | 0.908 |
| EO20 | 0.959 | 0.896 | 0.868 | 0.928 | |
| EO21 | 0.820 | 0.788 | 0.940 | ||
| H19 | 0.893 | 0.874 | |||
| H20 | 0.870 |
EO20—essential oil active compounds from 2020, EO21—essential oil active compounds from 2021, H19—hydrolate active compounds from 2019, H20—hydrolate active compounds from 2020, H21—hydrolate active compounds from 2021.
Table 6.
Comparison results of main L. x intermedia essential oil components and ISO 8902 standard.
| No. | Cultivar | Origin | Limonene | 1,8-cineole | Cis-Β-Ocimene | Trans-Β-Ocimene | Camphor | Linalool | Linalyl Acetate | Terpinene-4-Ol | Lavandulyl Acetate | Lavandulol | Reference |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Hidcote Giant | Canada | 1.4 | 17.1 | 7.8 | 12.2 | 13.3 | 23.8 | 0.3 | 4.1 | 0.0 | 1.6 | [19] |
| 2 | Grosso | Canada | 2.0 | 10.7 | 3.4 | 4.4 | 10.8 | 30.6 | 8.3 | 3.3 | 3.7 | 0.0 | [19] |
| 3 | Super | Canada | 2.7 | 13.1 | 3.5 | 6.2 | 5.5 | 28.3 | 10.0 | 0.0 | 3.4 | 0.0 | [19] |
| 4 | OK-Farms Super | Canada | 2.4 | 4.8 | 3.1 | 8.0 | 2.1 | 35.2 | 11.6 | 0.0 | 1.8 | 0.0 | [19] |
| 5 | French Super | Canada | 2.5 | 5.2 | 4.0 | 9.8 | 4.8 | 25.2 | 12.2 | 0.5 | 2.7 | 0.0 | [19] |
| 6 | Super | Turkey | 0.0 | 2.6 | 0.0 | 0.0 | 4.8 | 34.0 | 47.7 | 0.0 | 0.0 | 0.0 | [49] |
| 7 | n.s. | Macedonia | 0.0 | 6.7 | 2.3 | 0.8 | 6.6 | 39.0 | 2.1 | 5.1 | 1.9 | 1.7 | [11] |
| 8 | Dutch | Turkey | 1.1 | 7.6 | 0.0 | 0.0 | 11.3 | 44.8 | 8.2 | 1.5 | 0.0 | 0.1 | [50] |
| 9 | Giant Hidcote | Turkey | 1.3 | 12.0 | 0.0 | 0.0 | 6.6 | 41.6 | 3.9 | 1.5 | 0.0 | 0.2 | [50] |
| 10 | Super A | Turkey | 0.8 | 2.1 | 0.0 | 0.0 | 7.5 | 38.1 | 36.2 | 0.5 | 0.0 | 0.0 | [50] |
| 11 | Super A | Turkey | 0.4 | 3.2 | 1.5 | 6.6 | 43.7 | 24.6 | 5.4 | 0.0 | 0.0 | [51] | |
| 12 | Sumiens | Italy | 0.7 | 12.0 | 1.9 | 1.0 | 7.1 | 40.4 | 9.9 | 0.2 | 0.3 | 0.0 | [3] |
| 13 | Super A | Italy | 0.6 | 6.9 | 1.3 | 0.7 | 6.6 | 36.2 | 18.4 | 3.3 | 4.5 | 0.1 | [3] |
| 14 | Grosso | Italy | 0.5 | 8.1 | 1.2 | 0.8 | 8.1 | 38.4 | 15.7 | 3.6 | 4.1 | 0.1 | [3] |
| 15 | Super | Turkey | 0.7 | 0.0 | 2.6 | 1.5 | 5.3 | 36.8 | 33.1 | 0.0 | 1.2 | 0.1 | [4] |
| 16 | Grey Hedge | Turkey | 2.3 | 0.0 | 1.8 | 5.0 | 6.4 | 28.5 | 4.6 | 6.9 | 0.8 | 0.5 | [4] |
| 17 | Budrovka | Croatia | 4.0 | 8.4 | 0.0 | 0.4 | 0.1 | 57.1 | 9.8 | 2.3 | 0.2 | 1.1 | [9] |
| 18 | Grosso | Turkey | 2.5 | 33.1 | 0.0 | 0.0 | 21.8 | 0.2 | 0.0 | 1.0 | 0.2 | 0.4 | [18] |
| 19 | Dutch | Turkey | 2.9 | 35.8 | 0.0 | 0.0 | 22.2 | 0.2 | 0.0 | 0.8 | 0.1 | 0.2 | [18] |
| 20 | Abriel | Turkey | 2.5 | 35.9 | 0.0 | 0.0 | 22.8 | 0.1 | 0.0 | 1.0 | 0.2 | 0.1 | [18] |
| 21 | Grosso | Italy | 1.0 | 5.2 | 0.9 | 0.7 | 6.0 | 41.6 | 23.0 | 4.8 | 3.2 | 0.0 | [52] |
| 22 | n.s. | Serbia | 0.4 | 14.6 | 0.4 | 0.0 | 16.3 | 23.1 | 10.0 | 0.7 | 2.6 | 0.9 | [53] |
| 23 | Grappenhall | Hungary | 1.4 | 9.0 | 9.3 | 3.4 | 2.8 | 46.8 | 3.3 | 3.0 | 0.9 | 1.2 | [10] |
| 24 | Grosso | Hungary | 0.7 | 3.0 | 4.1 | 1.9 | 14.8 | 55.2 | 4.4 | 0.9 | 1.2 | 1.4 | [10] |
| 25 | n.s. | China | 0.0 | 44.8 | 0.0 | 0.5 | 12.2 | 7.6 | 0.0 | 0.6 | 0.0 | 0.0 | [21] |
| 26 | Abrialis | Italy | 0.5 | 7.0 | 3.0 | 8.3 | 9.4 | 40.3 | 18.4 | 0.6 | 1.4 | 0.8 | [54] |
| 27 | Rinaldi Cerioni | Italy | 0.4 | 10.0 | 0.0 | 0.0 | 11.5 | 65.8 | 0.5 | 2.9 | 0.0 | 0.6 | [54] |
| 28 | Sumiens | Italy | 0.7 | 12.1 | 3.0 | 0.4 | 6.8 | 48.0 | 14.9 | 0.3 | 0.0 | 0.0 | [54] |
| 29 | Budrovka | Serbia | 1.0 | 16.1 | 1.8 | 0.2 | 4.1 | 39.0 | 6.9 | 5.1 | 0.7 | 0.0 | TS |
| AVERAGE | 1.3 | 12.0 | 2.0 | 2.4 | 9.1 | 34.1 | 11.6 | 2.1 | 1.2 | 0.4 | |||
| RANGE | ≤4.0 | ≤44.8 | ≤9.3 | ≤12.2 | 0.1–22.8 | 0.1–65.8 | ≤47.7 | ≤6.9 | ≤4.5 | ≤1.7 | |||
| ISO STANDARD | 0.5–1.5 | 4.0–7.0 | 0.5–1.5 | tr-1.0 | 6.0–8.0 | 24.0–35.0 | 28.0–38.0 | 1.5–5.0 | 1.5–3.0 | 0.2–0.8 |
n.s.—not specified; TS—this study (average value for three investigated year).
Table 7.
Chemical composition of different Lavandula sp. hydrolates according to literature and this study.
Table 7.
Chemical composition of different Lavandula sp. hydrolates according to literature and this study.
| No. | Species/ Variety/ Cultivar | Origin | Extraction Technique/ Plant Material | Linalool | 1,8-cineole | Linalool Oxides | α-terpineol | Camphor | Borneol | Terpinen-4-Ol | Geraniol | Reference |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | LI cv “Super” | Turkey | f.f., SD | 55.6 | 9.8 | 6.0 | 0.0 | 13.4 | 13.5 | 0.0 | 1.6 | [49] |
| 2 | LO | Morocco | n.s. | 45.0 | 14.8 | 0.4 | 11.8 | 15.7 | 11.3 | 0.0 | 0.0 | [57] |
| 3 | LA | Poland | HV400 | 39.2 | 0.0 | 19.2 | 7.1 | 1.3 | 4.8 | 4.6 | 2.9 | [58] |
| 4 | LA | Poland | HV3200 | 29.0 | 0.0 | 19.1 | 12.7 | 3.0 | 9.3 | 6.9 | 0.0 | [58] |
| 5 | LA | Poland | f.h. | 53.0 | 2.9 | 0.4 | 8.3 | 0.9 | 5.3 | 3.9 | 4.0 | [55] |
| 6 | LA | Poland | d.h. | 48.0 | 2.7 | 0.6 | 8.8 | 1.5 | 5.8 | 6.6 | 5.0 | [55] |
| 7 | LA | Poland | f.f. | 43.6 | 4.4 | 1.0 | 7.5 | 0.0 | 6.6 | 5.6 | 3.4 | [55] |
| 8 | LA | Poland | d.f. | 39.2 | 0.0 | 19.2 | 7.0 | 1.3 | 4.8 | 4.6 | 2.9 | [55] |
| 9 | LA | Poland | d.f., VH400 | 43.6 | 4.4 | 0.0 | 7.5 | 0.0 | 6.6 | 5.6 | 0.0 | [28] |
| 10 | LA | Poland | d.f., VH800 | 44.9 | 2.0 | 0.0 | 8.5 | 1.3 | 5.2 | 5.4 | 3.4 | [28] |
| 11 | LA | Poland | d.f., VH1200 | 43.9 | 4.0 | 1.2 | 5.8 | 2.7 | 4.0 | 3.9 | 4.5 | [28] |
| 12 | LA | Poland | d.f., VH1600 | 25.7 | 2.7 | 1.4 | 4.1 | 1.0 | 4.6 | 3.7 | 1.1 | [28] |
| 13 | LI | Italy | f.f. | 43.8 | 25.4 | 0.1 | 1.8 | 12.8 | 4.3 | 4.5 | 0.0 | [30] |
| 14 | LI | Italy | f.s. | 34.4 | 28.9 | 0.0 | 2.2 | 15.4 | 4.0 | 2.7 | 0.2 | [30] |
| 15 | LI | Serbia | d.f. | 7.7 | 6.8 | 67.3 | 2.7 | 7.2 | 0.0 | 0.4 | 0.0 | [53] |
| 16 | LA | Croatia | d.f., SD | 7.9 | 20.6 | 21.0 | 10.4 | 0.4 | 0.0 | 1.1 | 1.0 | [56] |
| 17 | LA | Croatia | d.f., HD | 23.2 | 19.5 | 13.8 | 13.0 | 0.5 | 0.0 | 1.2 | 2.3 | [56] |
| 18 | LI cv „Grosso“ | Italy | n.s. | 12.6 | 52.9 | 0.7 | 4.8 | 19.6 | 3.0 | 5.4 | 0.0 | [29] |
| 19 | LA | Italy | f., SD | 42.9 | 11.8 | 0.1 | 12.6 | 18.4 | 5.8 | 8.4 | 0.0 | [31] |
| 20 | LI cv „Budrovka“ | Serbia | f.f., SD | 26.7 | 17.8 | 11.3 | 4.0 | 6.0 | 17.3 | 9.4 | 0.3 | TS |
| AVERAGE | 35.5 | 11.6 | 9.1 | 7.0 | 6.1 | 5.8 | 4.2 | 1.6 | ||||
| RANGE | 7.7–55.6 | ≤52.9 | ≤67.3 | ≤13.0 | ≤19.6 | ≤17.3 | ≤9.4 | ≤5.0 |
LI—Lavandula × intermedia; LO—Lavandula officinalis; LA—Lavandula angustifolia; TS—This Study (average values for all three years); VH—Volume of Hydrosols in ml; SD—Steam Distillation; HD—Hydro Distillation; n.s.—not specified; f.f.—fresh flowers; f.h.—fresh herb; d.h.—dry herb; d.f.—dry flower; f.s.—fresh stem; f.—flowers.
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Aćimović, M.; Lončar, B.; Stanković Jeremić, J.; Cvetković, M.; Pezo, L.; Pezo, M.; Todosijević, M.; Tešević, V. Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality. Horticulturae 2022, 8, 281. https://doi.org/10.3390/horticulturae8040281
AMA Style
Aćimović M, Lončar B, Stanković Jeremić J, Cvetković M, Pezo L, Pezo M, Todosijević M, Tešević V. Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality. Horticulturae. 2022; 8(4):281. https://doi.org/10.3390/horticulturae8040281
Chicago/Turabian StyleAćimović, Milica, Biljana Lončar, Jovana Stanković Jeremić, Mirjana Cvetković, Lato Pezo, Milada Pezo, Marina Todosijević, and Vele Tešević. 2022. "Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality" Horticulturae 8, no. 4: 281. https://doi.org/10.3390/horticulturae8040281
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