Resistance Management through Brassica Crop–TuMV–Aphid Interactions: Retrospect and Prospects

Round 1

Reviewer 1 Report

The manuscript ‘Resistance Management through Brassica crop-TuMV-Aphid Interactions: Retrospect and Prospects’, by Lu and collaborators, is a review that addresses virus-vector-host interactions. However, as proposed in the title, it deals very little with the resistance management of brassicas.

Furthermore, the authors do not propose management measures based on current knowledge of these interactions, using techniques such as RNAi and CRISPR-Cas9, for example. Another important issue that was missed was the aphid effectors. They can trigger molecular responses in the plant favoring (or not) insect feeding. In addition, many texts do not address the theme that is proposed in their title, containing only loose information without connection. The manuscript also has a lot of repeated information, for example, p. 3, lines 118-120 and 140-142. This is repeated elsewhere in the text.

Lastly, the manuscript needs to undergo complete English proofreading, as it has many meaningless sentences, wrong verb conjugations, and sentences with no connection to the previous ones. For these reasons, this manuscript should not be accepted in its current form.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript “Resistance Management through Brassica crop-TuMV-Aphid interactions: Retrospect and Prospects” provides a review of Brassica crop-TuMV-Aphid interactions. This review provides somewhat an extension to previous reviews on Brassica crop-TuMV and TuMV-Aphid interactions. Although the Authors did a better job on some parts of this review, there are more sections that need further improvements. I have seen many sentences/paragraphs with less clear and not have a better flow in your writing. Authors also need to pay special attention to citing more recent literature as well.

In the following, I will point out specific comments.

Please use Turnip mosaic virus when first mentioned.

Abstract:

Line 10: Delete “our country” here. Just mentioned “serious losses to the world which include China”. 

Line 10: Include order and family for aphids; please use the same format if you first find an insect species in your text

Line 11: revise your sentence for clarity, not clear how difficult to control aphids because of TuMV

Line 13: Use pesticides instead of chemical control

Line 14: what are other problems here . . . Be specific about this. .

Line 15: delete would

Line 16: you already mentioned these interactions. So delete “and clear the link among brassica crop, TuMV, and aphid”

Line 19: you already mentioned pest management, deleting control from aphids and TuMV

 

Introduction:

Line 26-28: Revise your sentence for clarity.

Line 33: should be one of the most effective measures not most effective measure

Line 35: delete many

Line 39: please provide more examples for hemipteran and other virus vectors such as thrips, mites, mealybugs, leafhoppers, planthoppers, etc.

Line 56-58: Incomplete sentence

Line 58: Please provide the year of TuMV first identification

Line 66: what is eIF4E stand for?

Line 68: which plant transport tissue? Is it phloem or xylem? Be specific

Line 59-68: If possible illustrate this mechanism in a schematic diagram, so that reader can easily get it.

Line 70-71: you have to provide a clear explanation of A and B genomes  

Line 80: Introduce eIF4F complex first; and then give all other details . . .

Line 83: what is the difference between eIF4E and eIF(iso)4E? Not clear to me

Line 87: what are these TuMV C4/CDN1/UK1/CHN2/CHN3 isolates stand for? Please provide the importance of these interactions

Line 70-90: You need to organize this all information in a logical way. Please revise this paragraph for clarity.

Line 98-99: revise this sentence for clarity.

Line 100-101: how about green leaf volatiles (C6)

Line 102-103: when leaves are mechanically wounded, injured by pathogens or damaged by herbivores . . .

Line 103: delete GLV at the end of this sentence

Line 104: what are those C9 GLVs? I never heard of . . .

Line 104-107: You are trying to introduce some facts on the biosynthesis of GLVs but not clear to me how they synthesize based on your explanation

Line 108: Delete “Normally “ here.

Any green plant will produce a sufficient amount of GLVs but this can enhance by biotic stressors. These volatile cues are benefited by natural enemies of herbivores.

Line 98-110: Brassica crops have significant production of glucosinolates. You didn’t provide any information on that. Is glucosinolates production enhanced by plant viruses? Did you find any relevant literature on that? Please provide details

Line 112-113: Not clear about how this works “Aphid infection increases the susceptibility of plants”

Line 120: what do you mean by high sweetness? Please cite this information

 

Look at these papers for more information on aphid olfaction:

Identification and functional characterization of an odorant receptor in pea aphid, Acyrthosiphon pisum

by Rui-Bin Zhang et al 2019

 

The role of olfaction in aphid host location

by Ben et al 2012

 

Orco mediates olfactory behaviors and winged morph differentiation induced by alarm pheromone in the grain aphid, Sitobion avenae

By Fan et al 2015

 

Line 128: delete kind of

Line 131: what is phytoprotectin? Define this before introducing a new term.

Line 133: use volatile organic compounds (VOCs) instead of volatile substances throughout this manuscript

Line 134-135: specify those secondary metabolites and proteins

Line 136-137: provide an example for indirect defense

Line 140: Are you referring to alate and apterous aphids?

Line 142: provide examples and cite that information

Line 143: should be constitutive and induced VOCs

Line 139: The title should focus mainly on aphids not generally insects

Line 147: plant VOCs could influence searching mates, host selection, foraging, and egg-laying decisions.

For example, a low number of eggs were laid on clubroot infected canola plants due to the change in VOC profile.

Recently published reference:

Infection of canola by the root pathogen Plasmodiophora brassicae increases resistance to aboveground herbivory by bertha armyworm, Mamestra configurata Walker (Lepidoptera: Noctuidae)

Weeraddana et al 2020; plant science; https://doi.org/10.1016/j.plantsci.2020.110625

Line 148: is it a VOC mixture?

Line 150-151: revise your sentence for clarity; should be plant volatile organic compounds (VOCs)

Line 153: delete healthy and insert uninfested or insect-free plants

Line 155: Not only plants were previously infested with herbivores, but also pathogens. For example, there is information on insects choosing uninoculated over pathogen inoculated canola for oviposition.

Reference:

Weeraddana et al 2020; plant science; https://doi.org/10.1016/j.plantsci.2020.110625

Line 156: In the first mention, please provide a common name (order: family) for any insect species you described

Example, diaeretiella rapae

Line 157: which aphid? Is it a specialist or generalist?

Line 160-169: this paragraph should be placed before speaking about the attraction of natural enemies in 157-158.

Line 175-176: delete eat and insert feed

Line 183-185: another way can be reducing the aphid piercing damage. For example, brown seaweed extract treated plants had a lower amount of piercing damage compared to control.

Reference:

Weeraddana, C.D., Kandasamy, S., Cutler, G.C. et al. An alkali-extracted biostimulant prepared from Ascophyllum nodosum alters the susceptibility of Arabidopsis thaliana to the green peach aphid. J Appl Phycol 33, 3319–3329 (2021). https://doi.org/10.1007/s10811-021-02534-9

 

Line 175-176: Cite this paper for emission of VOCs following TuMV in Brassica

Lu, X.; Zhang, L.; Huang, W.; Zhang, S.; Zhang, S.; Li, F.; Zhang, H.; Sun, R.; Zhao, J.; Li, G. Integrated Volatile Metabolomics and Transcriptomics Analyses Reveal the Influence of Infection TuMV to Volatile Organic Compounds in Brassica rapa. Horticulturae 2022, 8, 57. https://doi.org/10.3390/horticulturae8010057

 

Line 191: please focus mainly on aphids as there are so many papers on this topic

Line 205: Brassicaceae, formerly is Cruciferae

Line 228: Please provide full names and then use these acronyms within brackets (AUX, GA, CTK, BR and ABA)

Line 236: SA could influence specifically for biotrophic pathogens. For example, biotrophic pathogen, Plasmodiophora brassicae infection induced higher SA defenses in canola.

Reference:

Weeraddana et al 2020; plant science; https://doi.org/10.1016/j.plantsci.2020.110625

Line 255: delete proved

Line 241-262: I see you provided a better description on hormone signalling involvement in virus-host plant interaction

Line 271-300: you need to provide more descriptions on different mechanisms involved with environmental factors that could affect brassica crop-TuMV-aphid interactions

See this paper:

Honjo, M.N., Emura, N., Kawagoe, T. et al. Seasonality of interactions between a plant virus and its host during persistent infection in a natural environment. ISME J 14, 506–518 (2020). https://doi.org/10.1038/s41396-019-0519-4

 

Line 303: delete “drugs”; just use chemicals instead

Line 305: use sustainable aphid management instead green aphid control

Line 301-334: you did a great job on the prospects section. I have no comments on that.

 

References:

 

I see many formatting errors in the reference section. Check all references and format according to this Journal.

I have seen many errors, here I mentioned a few for your attention

Line 357: The first letter of the title capitalized some are not like that; space between initials

Line 369: scientific names are not italicized throughout the manuscript

Line 389: Journal abbreviation should be Acta Hortic. Sin.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 3 Report

General remarks

The work, if published, may constitute a serious impulse to intensify research on the dangerous phytosanitary problem, which is the Brasica crop-Turic mosaic virus (TuMV) -Aphids biosystem, not only in China, but all over the world. The problem is related to food issues, among which Brassica spp. is an important crop. On the other hand, we have a very dangerous pathogen and a dangerous vector - Aphids.

An in-depth study of these relationships is the basis for the control of pathogens and pests in modern crop protection. Recently, it has been emphasized that the greatest successes in this field can be achieved through the so-called management of crop resistance to pathogens and pests.

Detailed comments

TuMV virus causes serious diseases of cabbage cultivation in the Far East, including China and the Northern Hemisphere. Not only are the vectors of this disease important - aphids (approx. 90 species), but also the influence of the environment (including anthropogenic factors). Chemical control - temporarily effective, cannot be the only solution here, if only because of the harmfulness to the environment, as well as pesticide residues in food products.

Author Response

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Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

The manuscript “Resistance Management through Brassica crop-TuMV-Aphid interactions: Retrospect and Prospects” provide a review on Brassica crop-TuMV-Aphid interactions. This review provides extension to previous reviews on Brassica crop-TuMV and TuMV-Aphid interactions.

The authors have responded to nicely revised the manuscript according to the reviewer comments. I still have few more minor changes to include prior to publication. Specific questions/changes are stated below.

Line 54: there are too many “and: within a sentence. Maybe use semicolon ; between each statement

Line 72: define CP protein before use acronym

Line 190: KUŚNIERCZYK et al., is capitalized here

Line 213-214: Reference 67 in the revised manuscript: this work is belongs to a noctuid moth species, bertha armyworm NOT aphids. You could change aphid to insect here OR delete this from text OR cite it here

“It could influence searching mates, host selection, foraging, and egg-laying decisions” (cite this here as the outcome of this work was influence on egg laying decisions)

Line 223: Revise this sentence for clarity

Line 231-245: Over use “ on the other hand. Delete one of them

Line 409-410: revise this sentence for clarity

Line 415-416: revise this sentence for clarity; “without doing anything”

Line 435: replace “ hot topic” with major topic

Line 436: change this similar to this: some questions need further investigation:

Author Response

Please see the attachment

Author Response File: Author Response.pdf