PdStuA Is a Key Transcription Factor Controlling Sporulation, Hydrophobicity, and Stress Tolerance in Penicillium digitatum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Culture Conditions
2.2. Targeted Gene Disruption and Complementation
2.3. Phylogenetic Analysis
2.4. Phenotypic Analysis and Subcellular Organelle Observation
2.5. Stress Tolerance Assays
2.6. Virulence Assays
2.7. Quantitative Real-Time PCR Assays (RT-qPCR)
2.8. Statistical Analysis
3. Results
3.1. Summary of PdStuA in P. digitatum
3.2. Construction and Verification of PdStuA-Deletion and PdStuA-Complementation Mutants
3.3. PdStuA Affects Spore Production but Does Not Affect Hyphal Development
3.4. PdStuA Controls the Occurrence of Conidiophores
3.5. PdStuA Is Involved in Hyphal Hydrophobicity in P. digitatum
3.6. PdStuA Takes Part in Cell Wall Integrity
3.7. PdStuA Is Essential for Stress Responses
3.8. Expression Change for Transcription
3.9. PdStuA Does Not Affect the Pathogenicity of P. digitatum
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Du, Y.; Zhu, J.; Tian, Z.; Long, C. PdStuA Is a Key Transcription Factor Controlling Sporulation, Hydrophobicity, and Stress Tolerance in Penicillium digitatum. J. Fungi 2023, 9, 941. https://doi.org/10.3390/jof9090941
Du Y, Zhu J, Tian Z, Long C. PdStuA Is a Key Transcription Factor Controlling Sporulation, Hydrophobicity, and Stress Tolerance in Penicillium digitatum. Journal of Fungi. 2023; 9(9):941. https://doi.org/10.3390/jof9090941
Chicago/Turabian StyleDu, Yujie, Jinfan Zhu, Zhonghuan Tian, and Chaoan Long. 2023. "PdStuA Is a Key Transcription Factor Controlling Sporulation, Hydrophobicity, and Stress Tolerance in Penicillium digitatum" Journal of Fungi 9, no. 9: 941. https://doi.org/10.3390/jof9090941