Evaluation of the Potential Entomopathogenic Fungi Purpureocillium lilacinum and Fusarium verticillioides for Biological Control of Forcipomyia taiwana (Shiraki)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Forcipomyia Taiwana Collection and Rearing
2.2. Fungal Isolation and Selection
2.3. Molecular Identification
2.4. Phylogenetic Analysis
2.5. Thermotolerance Assay
2.6. Conidia Productivity
2.7. Ranking of EPF Isolates by Effective Conidia Number (ECN)
2.8. Virulence Screening against F. taiwana Last Instar Larvae
2.9. Bioassay of EPF against Different Life Stages of F. taiwana
2.10. Statistical Analysis
3. Results
3.1. Isolation and Selection of Entomopathogenic Fungi
3.2. Molecular Identification
3.3. ECN Ranking of the Entomopathogenic Fungi
3.4. Virulence Screening against F. taiwana Larvae
3.5. Bioassay of EPF against Different Life Stages of F. taiwana
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ni, N.-T.; Wu, S.-S.; Liao, K.-M.; Tu, W.-C.; Lin, C.-F.; Nai, Y.-S. Evaluation of the Potential Entomopathogenic Fungi Purpureocillium lilacinum and Fusarium verticillioides for Biological Control of Forcipomyia taiwana (Shiraki). J. Fungi 2022, 8, 861. https://doi.org/10.3390/jof8080861
Ni N-T, Wu S-S, Liao K-M, Tu W-C, Lin C-F, Nai Y-S. Evaluation of the Potential Entomopathogenic Fungi Purpureocillium lilacinum and Fusarium verticillioides for Biological Control of Forcipomyia taiwana (Shiraki). Journal of Fungi. 2022; 8(8):861. https://doi.org/10.3390/jof8080861
Chicago/Turabian StyleNi, Nian-Tong, Sing-Shan Wu, Kuei-Min Liao, Wu-Chun Tu, Chuen-Fu Lin, and Yu-Shin Nai. 2022. "Evaluation of the Potential Entomopathogenic Fungi Purpureocillium lilacinum and Fusarium verticillioides for Biological Control of Forcipomyia taiwana (Shiraki)" Journal of Fungi 8, no. 8: 861. https://doi.org/10.3390/jof8080861