Pulmonary Conventional Type 1 Langerin-Expressing Dendritic Cells Play a Role in Impairing Early Protective Immune Response against Cryptococcus neoformans Infection in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Cryptococcus neoformans
2.3. Pulmonary Infection and Langerin-Expressing Cell Depletion in LangEGFPDTR Mice
2.4. Histology
2.5. Fungal Burden
2.6. Cultures of Pulmonary Cells
2.7. Flow Cytometry Analysis
2.8. Cytokine Production
2.9. Fungal Growth Inhibition by Lung Cells
2.10. Arginase Activity Assay
2.11. Immunofluorescence
2.12. Statistical Analysis
3. Results
3.1. LangDC1 Depletion Favors the Pulmonary Clearance of C. neoformans at the Early Stage of Infection
3.2. LangDC1 Downregulate Cytokine Production and Antifungal Activity of Lung Cells during the Early Stage of Cryptococcal Infection
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Guasconi, L.; Beccacece, I.; Volpini, X.; Burstein, V.L.; Mena, C.J.; Silvane, L.; Almeida, M.A.; Musri, M.M.; Cervi, L.; Chiapello, L.S. Pulmonary Conventional Type 1 Langerin-Expressing Dendritic Cells Play a Role in Impairing Early Protective Immune Response against Cryptococcus neoformans Infection in Mice. J. Fungi 2022, 8, 792. https://doi.org/10.3390/jof8080792
Guasconi L, Beccacece I, Volpini X, Burstein VL, Mena CJ, Silvane L, Almeida MA, Musri MM, Cervi L, Chiapello LS. Pulmonary Conventional Type 1 Langerin-Expressing Dendritic Cells Play a Role in Impairing Early Protective Immune Response against Cryptococcus neoformans Infection in Mice. Journal of Fungi. 2022; 8(8):792. https://doi.org/10.3390/jof8080792
Chicago/Turabian StyleGuasconi, Lorena, Ignacio Beccacece, Ximena Volpini, Verónica L. Burstein, Cristian J. Mena, Leonardo Silvane, Mariel A. Almeida, Melina Mara Musri, Laura Cervi, and Laura S. Chiapello. 2022. "Pulmonary Conventional Type 1 Langerin-Expressing Dendritic Cells Play a Role in Impairing Early Protective Immune Response against Cryptococcus neoformans Infection in Mice" Journal of Fungi 8, no. 8: 792. https://doi.org/10.3390/jof8080792