The Biogenically Efficient Synthesis of Silver Nanoparticles Using the Fungus Trichoderma harzianum and Their Antifungal Efficacy against Sclerotinia sclerotiorum and Sclerotium rolfsii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Causal Agent and Bioagent
2.2. Extracellular Synthesis and Characterization of AgNs
2.3. Antifungal Activity of AgNs against S. sclerotiorum and S. rolfsii
2.3.1. Crude Culture Filtrate (CF)
2.3.2. Effect of AgNs on Some Growth Parameters of S. sclerotiorum and S. rolfsii
2.3.3. Effect of AgNs on the Mycelial Dry Weight of S. sclerotiorum and S. rolfsii
2.3.4. Effects of AgNs on Root and Crown Rot Severity Caused by S. sclerotiorum
2.3.5. Disease Assessment
2.4. In Vivo Effects of AgNs Foliar Spraying on Sunflower Plants Infected by S. rolfsii
2.5. Statistical Analysis
3. Results
3.1. Biosynthesis and Characterization of AgNs
3.2. Antifungal Activity of AgNs
3.2.1. In Vitro Effect of AgNs on the Growth of Sclerotinia sclerotiorum and Sclerotium rolfsii
3.2.2. Mycelial Dry Weight (DW)
3.2.3. Number of Sclerotia
3.2.4. In Vivo Effect of AgNs on the Severity of Root Rot Disease
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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El-Ashmony, R.M.S.; Zaghloul, N.S.S.; Milošević, M.; Mohany, M.; Al-Rejaie, S.S.; Abdallah, Y.; Galal, A.A. The Biogenically Efficient Synthesis of Silver Nanoparticles Using the Fungus Trichoderma harzianum and Their Antifungal Efficacy against Sclerotinia sclerotiorum and Sclerotium rolfsii. J. Fungi 2022, 8, 597. https://doi.org/10.3390/jof8060597
El-Ashmony RMS, Zaghloul NSS, Milošević M, Mohany M, Al-Rejaie SS, Abdallah Y, Galal AA. The Biogenically Efficient Synthesis of Silver Nanoparticles Using the Fungus Trichoderma harzianum and Their Antifungal Efficacy against Sclerotinia sclerotiorum and Sclerotium rolfsii. Journal of Fungi. 2022; 8(6):597. https://doi.org/10.3390/jof8060597
Chicago/Turabian StyleEl-Ashmony, Ranya M. S., Nouf S. S. Zaghloul, Marija Milošević, Mohamed Mohany, Salim S. Al-Rejaie, Yasmine Abdallah, and Anwar A. Galal. 2022. "The Biogenically Efficient Synthesis of Silver Nanoparticles Using the Fungus Trichoderma harzianum and Their Antifungal Efficacy against Sclerotinia sclerotiorum and Sclerotium rolfsii" Journal of Fungi 8, no. 6: 597. https://doi.org/10.3390/jof8060597