European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Panel Description
2.2. Participating Laboratories
2.3. 2015 Sera Panel
2.4. 2018 and 2022 Sera Panel
2.5. EURL CFT Protocol
- -
- HI of 0 or trace: Negative serum (Neg),
- -
- HI of 1+: Suspicious serum (Susp),
- -
- HI of 2+, 3+, 4+: Positive serum (Pos),
- -
- serum with anti-complementary activity: Inconclusive serum (IC).
2.6. Analytical Methods
3. Results
3.1. Dourine CFT ILPT Organisation and Panel Samples
3.2. Analysis of Results from the Dourine CFT ILPT Participants
3.3. Analysis of Quantitative Results per Serum
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nbr of Repetitions per Panel | ||||||||
---|---|---|---|---|---|---|---|---|
Sample Origin | Serum ID | Dilution | Form | Dourine CFT Expected Result | Dourine CFT Titres from EURL Tests | 2015 | 2018 | 2022 |
Mix of negative horse sera | Neg 1 | - | Native | Negative | 0 | 2 | ||
Horse immunisation with T. b. equiperdum OVI antigens | HT | - | Native | Positive | 60 | 1 | ||
Horse immunisation with T. b. equiperdum OVI antigens | MT | - | Native | Positive | 8 | 1 | ||
LT | 3/5 dilution of MT serum | Native | Positive | 5 | 2 | |||
Horse immunisation with T. b. equiperdum Teva antigens | Teva N | - | Native | Positive | 80 | 1 | ||
Horse immunisation with T. b. evansi Rotat 1.2 antigens | Rotat 1.2 N | - | Native | Positive | 40 | 1 | ||
Horse experimental infection with T. b. equiperdum Ethiopian strain | Ethiopian | - | Native | Positive | 20 | 2 | ||
Mix of negative horse sera | Neg 2 | - | Freeze-dried | Negative | 0 | 7 | 3 | |
Horse immunisation with T. b. equiperdum Teva antigens | Teva | - | Freeze-dried | Positive | 80 | 3 | 2 | |
Teva (1/2) | 1/2 dilution of Teva serum | Freeze-dried | Positive | 40 | 2 | |||
Teva (1/4) | 1/4 dilution of Teva serum | Freeze-dried | Positive | 20 | 3 | 2 | ||
Horse immunisation with T. b. evansi Rotat 1.2 antigens | Rotat 1.2 | - | Freeze-dried | Positive | 60 | 1 | 1 | |
Rotat 1.2 (1/2) | 1/2 dilution of Rotat 1.2 serum | Freeze-dried | Positive | 30 | 2 | |||
Rotat 1.2 (1/4) | 1/4 dilution of Rotat 1.2 serum | Freeze-dried | Positive | 15 | 2 | |||
Horse experimental infection with T. b. equiperdum OVI strain | Trypeq | - | Freeze-dried | Positive | 80 | 2 | ||
Number of samples per ILPT: | 10 | 20 | 10 |
2015 | 2018 | 2022 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
# of Unexpected Results | Successfully Identified Samples (%) | Antigen b | Antigen Dilution | # of Unexpected Results | Successfully Identified Samples (%) | Antigen Provider | Antigen Dilution | # of Unexpected Results | Successfully Identified Samples (%) | Antigen Provider | Antigen Dilution | |
Participant 1 | 0 | 100 | A-1 | 60 | 0 | 100 | F-1 | 20 | 0 | 100 | F-2 | 16 |
Participant 2 | 0 | 100 | A-2 | 200 | 0 | 100 | A-11 | 100 | 0 | 100 | F-3 | 30 |
Participant 3 | 0 | 100 | A-3 | 100 | 0 | 100 | F-1 | 50 | 0 | 100 | F-2 | 40 |
Participant 4 | 0 | 100 | B-1 | 40 | 0 | 100 | B-1 | 30 | 0 | 100 | B-3 | 10 |
Participant 5 | 0 | 100 | A-4 | 64 | 0 | 100 | A-4 | 64 | 0 | 100 | F-3 | 48 |
Participant 6 | 0 | 100 | C-1 | 80 | 0 | 100 | C-2 | 70 | 0 | 100 | C-3 | 20 |
Participant 7 | 0 | 100 | A-1 | 100 | 0 | 100 | A-12 | 128 | 0 | 100 | A-15 | 200 |
Participant 8 | 0 | 100 | D-1 | 100 | 0 | 100 | D-2 | 180 | 0 | 100 | D-3 | 100 |
Participant 9 | 0 | 100 | A-5 | 100 | 0 | 100 | F-1 | 50 | 1 | 90 | A-16 | 100 |
Participant 10 | 0 | 100 | B-2 | 32 | 0 | 100 | B-2 | 32 | 3 | 70 | F-2 | 64 |
Participant 11 | 0 | 100 | A-6 | 128 | 7 | 65 | A-13 | 160 | 0 | 100 | F-3 | 10 |
Participant 12 | 0 | 100 | A-3 | 50 | 8 | 60 | F-1 | 1400 | - | - | - | - |
Participant 13 | 2 | 80 | A-7 | 50 | 0 | 100 | F-1 | 30 | 0 | 100 | F-2 | 30 |
Participant 14 | 2 | 80 | A-3 | 10 | 0 | 100 | F-1 | 40 | 0 | 100 | F-2 | 256 |
Participant 15 | 2 | 80 | A-8 | 100 | 0 | 100 | F-1 | 30 | 0 | 100 | F-2 | 30 |
Participant 16 | 3 | 70 | A-3 | 15 | 0 | 100 | F-1 | 60 | 0 | 100 | F-2 | 75 |
Participant 17 | 3 | 70 | A-9 | 40 | 0 | 100 | A-14 | 50 | 0 | 100 | F-2 | 20 |
Participant 18 | 3 | 70 | A-10 | 200 | 0 | 100 | F-1 | 30 | 0 | 100 | F-3 | 30 |
Participant 19 | 5 | 50 | E-1 | 128 | 0 | 100 | F-1 | 30 | 0 | 100 | A-17 | 20 |
Participant 20 | 5 | 50 | E-1 | 16 | - | - | - | - | - | - | - | - |
Participant 21 | 6 | 40 | E-1 | 32 | 0 | 100 | A-12 | 100 | 3 | 70 | A-18 | 9 |
Participant 22 | - a | - | - | - | - | - | - | - | 6 | 40 | F-3 | 10 |
% of laboratories obtaining 100% successful results | 57% | 90% | 80% |
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Hébert, L.; Froger, D.; Madeline, A.; Lecouturier, F.; Lemans, C.; Zientara, S. European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test. Vet. Sci. 2023, 10, 592. https://doi.org/10.3390/vetsci10100592
Hébert L, Froger D, Madeline A, Lecouturier F, Lemans C, Zientara S. European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test. Veterinary Sciences. 2023; 10(10):592. https://doi.org/10.3390/vetsci10100592
Chicago/Turabian StyleHébert, Laurent, Delphine Froger, Anthony Madeline, Fanny Lecouturier, Charlène Lemans, and Stephan Zientara. 2023. "European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test" Veterinary Sciences 10, no. 10: 592. https://doi.org/10.3390/vetsci10100592