Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Standards
2.2. Fish Protein Extraction
2.3. Simulated In Vitro Gastrointestinal Digestion
2.4. Hydrolyzed Peptide Purification
2.4.1. Medium-Sized Peptides Purification
2.4.2. Short-Sized Peptide Purification
2.5. Chromatography–Mass Spectrometry Analysis of Peptide Samples
2.5.1. Analysis of Medium-Sized Peptides by NanoHPLC–MS/MS
2.5.2. Analysis of Short-Sized Peptides by UHPLC–MS/MS
2.6. Qualitative Antimicrobial Assay
2.7. Quantitative Antimicrobial Assay—Minimum Inhibiting Concentration (MIC) Determination
2.8. Peptide Identification
2.8.1. Medium-Sized Peptide Identification
2.8.2. Short Peptide Identification
3. Results and Discussion
3.1. Antimicrobial Activity of C18 and GCB Digested Fractions
3.2. Characterization of Hydrolyzed Medium-Sized Peptides in Fish
3.3. Characterization of Hydrolyzed Short-Sized Peptides in Fish
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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C18 Extract | GCB Extract | |||
---|---|---|---|---|
Concentration (mg mL−1) | MIC (cfu mL−1) | OD600 | MIC (cfu mL−1) | OD600 |
7.0 | 0 | 0.000 | 0 | 0.000 |
3.5 | 0 | 0.000 | 0 | 0.000 |
1.0 | 0 | 0.000 | 1 | 0.021 |
0.5 | 3 | 0.018 | 3 | 0.023 |
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Cerrato, A.; Capriotti, A.L.; Capuano, F.; Cavaliere, C.; Montone, A.M.I.; Montone, C.M.; Piovesana, S.; Zenezini Chiozzi, R.; Laganà, A. Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion. Foods 2020, 9, 1185. https://doi.org/10.3390/foods9091185
Cerrato A, Capriotti AL, Capuano F, Cavaliere C, Montone AMI, Montone CM, Piovesana S, Zenezini Chiozzi R, Laganà A. Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion. Foods. 2020; 9(9):1185. https://doi.org/10.3390/foods9091185
Chicago/Turabian StyleCerrato, Andrea, Anna Laura Capriotti, Federico Capuano, Chiara Cavaliere, Angela Michela Immacolata Montone, Carmela Maria Montone, Susy Piovesana, Riccardo Zenezini Chiozzi, and Aldo Laganà. 2020. "Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion" Foods 9, no. 9: 1185. https://doi.org/10.3390/foods9091185