Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Standards
2.2. Plant Material
2.3. Chromatographic System
2.4. Extraction of AnAc from Cashew Peduncles
2.5. Structural Confirmation of AnAc by UPLC–QTOF-MSE
2.6. Validation Method
2.7. Selectivity
2.8. Linearity
2.9. Precision
2.10. Accuracy and Recovery
2.11. System Suitability Test
2.12. Limit of Detection and Quantification
3. Results
3.1. Method Development
3.2. Method Validation
3.3. Selectivity and Linearity
3.4. Precision and System Suitability Tests
3.5. LOD and LOQ
3.6. Accuracy or Recovery
3.7. Structural Confirmation of AnAc and Quantification of a Real Sample
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Analytical Curve | y = 2333.5x + 2956.2 | R2 = 0.998 | |||
---|---|---|---|---|---|
Angular Coefficient | A | Sa | tcal | ttab | t test |
2333.5 | 15.79 | 147.63 | 2.009 | Significant | |
Linear Coefficient | B | Sb | tcal | ttab | t test |
2956.2 | 830.91 | 3.56 | 2.009 | Significant | |
Fcalc | Ftab | F Test | |||
21,812.56 | 1.576 | Significant | |||
R2 | CV (%) | ||||
Intraday (n = 6) | 0.9993 | 0.20 | |||
Interday (n = 9) | 0.9979 | 0.29 | |||
Retention time (RSD) | Area (RSD) | ||||
System suitability test | 0.45 | 0.30 | |||
Concentration (µg·mL−1) | |||||
LOD | 0.18 | ||||
LOQ | 0.85 |
Sample | AnAc | Extraction (mg·100 g−1) | Total Amount (mg·100 g−1) | ||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | |||
CCP 09 | 15:3 | 22.07 ± 0.13 | 2.32 ± 0.01 | <LOQ | <LOQ | <LOQ | 24.40 ± 0.14 |
15:2 | 16.66 ± 0.02 | 1.81 ± 0.04 | <LOQ | <LOQ | <LOQ | 18.47 ± 0.02 | |
15:1 | 109.88 ± 0.42 | 14.63 ± 0.14 | 2.39 ± 0.09 | 0.81 ± 0.02 | <LOQ | 127.72 ± 0.48 | |
CCP 76 | 15:3 | 23.16 ± 0.06 | 6.34 ± 0.07 | 1.65 ± 0.01 | <LOQ | <LOQ | 31.16 ± 0.11 |
15:2 | 41.22 ± 0.41 | 12.01 ± 0.09 | 3.62 ± 0.05 | <LOQ | <LOQ | 56.87 ± 0.34 | |
15:1 | 79.98 ± 0.28 | 24.07 ± 0.33 | 7.74 ± 0.31 | 0.16 ± 0.04 | <LOQ | 111.96 ± 0.29 | |
BRS 265 | 15:3 | 39.88 ± 0.31 | 4.64 ± 0.06 | 0.23 ± 0.02 | <LOQ | <LOQ | 44.76 ± 0.32 |
15:2 | 33.29 ± 0.19 | 3.99 ± 0.03 | 0.10 ± 0.01 | <LOQ | <LOQ | 37.38 ± 0.22 | |
15:1 | 115.26 ± 0.65 | 16.14 ± 0.04 | 2.33 ± 0.10 | 1.12 ± 0.89 | <LOQ | 134.86 ± 1.14 | |
BRS 275 | 15:3 | 26.88 ± 0.25 | 2.95 ± 0.06 | <LOQ | <LOQ | <LOQ | 29.83 ± 0.29 |
15:2 | 17.51 ± 0.11 | 1.64 ± 0.10 | <LOQ | <LOQ | <LOQ | 19.15 ± 0.04 | |
15:1 | 81.26 ± 2.04 | 10.45 ± 0.19 | 1.42 ± 0.15 | 0.49 ± 0.18 | <LOQ | 93.63 ± 2.01 | |
Embrapa 51 | 15:3 | 16.56 ± 0.08 | 3.05 ± 0.01 | <LOQ | <LOQ | <LOQ | 19.62 ± 0.07 |
15:2 | 11.66 ± 0,05 | 2.05 ± 0.03 | <LOQ | <LOQ | <LOQ | 13.71 ± 0.06 | |
15:1 | 75.95 ± 0.82 | 16.56 ± 0.58 | 2.09 ± 0.03 | 0.40 ± 0.06 | <LOQ | 95.02 ± 1.10 |
Sample | AnAc | Recovery (%) | ||||
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | ||
CCP09 | 15:3 | 85.35 ± 0.49 | 11.42 ± 0.05 | 2.18 ± 0.16 | 0.93 ± 0.05 | 0.11 ± 0.05 |
15:2 | 83.88 ± 0.13 | 12.27 ± 0.22 | 2.56 ± 0.06 | 1.14 ± 0.06 | 0.13 ± 0.06 | |
15:1 | 84.51 ± 0.32 | 11.73 ± 0.11 | 2.18 ± 0.07 | 1.17 ± 0.02 | 0.19 ± 0.06 | |
CCP76 | 15:3 | 71.17 ± 0.19 | 21.05 ± 0.22 | 7.06 ± 0.04 | 0.66 ± 0.05 | 0.03 ± 0.02 |
15:2 | 70.48 ± 0.70 | 21.42 ± 0.15 | 7.30 ± 0.09 | 0.78 ± 0.10 | 0.02 ± 0.02 | |
15:1 | 70.25 ± 0.25 | 21.58 ± 0.29 | 7.36 ± 0.27 | 0.77 ± 0.03 | 0.02 ± 0.01 | |
BRS265 | 15:3 | 85.33 ± 0.66 | 11.26 ± 0.13 | 2.00 ± 0.05 | 1.33 ± 0.77 | 0.05 ± 0.04 |
15:2 | 84.97 ± 0.48 | 11.76 ± 0.08 | 2.03 ± 0.03 | 1.16 ± 0.74 | 0.06 ± 0.03 | |
15:1 | 84.15 ± 0.47 | 12.23 ± 0.03 | 2.21 ± 0.07 | 1.33 ± 0.65 | 0.06 ± 0.01 | |
BRS275 | 15:3 | 84.56 ± 0.79 | 11.33 ± 0.20 | 2.34 ± 0.10 | 1.54 ± 0.05 | 0.22 ± 0.05 |
15:2 | 84.41 ± 0.51 | 11.19 ± 0.47 | 1.90 ± 0.23 | 1.31 ± 0.07 | 0.18 ± 0.07 | |
15:1 | 84.73 ± 2.11 | 11.58 ± 0.19 | 2.24 ± 0.16 | 1.29 ± 0.19 | 0.15 ± 0.04 | |
Embrapa51 | 15:3 | 78.67 ± 0.36 | 17.24 ± 0.05 | 2.74 ± 0.03 | 1.17 ± 0.18 | 0.17 ± 0.02 |
15:2 | 78.24 ± 0.30 | 17.56 ± 0.18 | 2.73 ± 0.14 | 1.17 ± 0.20 | 0.28 ± 0.32 | |
15:1 | 78.13 ± 0.84 | 17.62 ± 0.59 | 2.88 ± 0.03 | 1.15 ± 0.06 | 0.20 ± 0.05 |
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Oiram Filho, F.; Mitri, M.P.; Zocolo, G.J.; Canuto, K.M.; de Brito, E.S. Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector. Foods 2023, 12, 2759. https://doi.org/10.3390/foods12142759
Oiram Filho F, Mitri MP, Zocolo GJ, Canuto KM, de Brito ES. Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector. Foods. 2023; 12(14):2759. https://doi.org/10.3390/foods12142759
Chicago/Turabian StyleOiram Filho, Francisco, Morgana Pereira Mitri, Guilherme Julião Zocolo, Kirley Marques Canuto, and Edy Sousa de Brito. 2023. "Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector" Foods 12, no. 14: 2759. https://doi.org/10.3390/foods12142759