Choline-Based Ionic Liquids as Media for the Growth of Saccharomyces cerevisiae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Synthesis of ILs
2.2.2. Microorganism and Culture Conditions
2.2.3. Minimum Inhibitory Concentration (MIC)
2.2.4. Screening of ILs Concentration
3. Results and Discussion
3.1. Toxicity of ILs
3.2. Growth Profile
3.3. Glucose Utilization Profile
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviation
emim[Cl] | 1-ethyl-3-methylimidazolium chloride |
bmim[Cl] | 1-butyl-3-methylimidazolium chloride |
emim[OAc] | 1-ethyl-3-methylimidazolium acetate |
emim[CH3PO4] | 1-ethyl-3-methylimidazolium methylphosphonate |
DMEAA | N,N-dimethylethanolammonium acetate |
[EtOHNMe3][Me2PO4] | 2-hydroxyethyl trimethyl ammonium dimethyl phosphate |
emim[MeO(H)PO2] | 1-ethyl-3-methylimidazolium methylphosphonate |
omimPF6 | 1-methyl-3-octylimidazolium hexafluorophosphate |
YPD | Yeast Extract-Peptone-Dextrose |
FDA | Food and Drug Administration |
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ILs | Molecular Weight, (g mol−1) | Physical Appearance | Thermal Properties | |||
---|---|---|---|---|---|---|
Cation | Anion | Abbreviation | TGA Decomposition Temperature, Tonset (°C) | DSC Glass Transition Temperature, Tg (°C) | ||
Choline | Tryptophanate | CT | 307.39 | Brown, viscous liquid | 187.55 | −26.88 |
Saccharinate | CS | 286.35 | Light yellow, viscous liquid | 248.82 | −62.33 | |
Phosphate | CDP | 201.16 | White, viscous liquid | 229.85 | −24.63 |
Experiment | Media Constituent | Ratio |
---|---|---|
1 | CT + CDHP + CS | 2:1:2 |
2 | Yeast Extract + CDHP + CS | 1:1:2 |
3 | CT + Peptone + CS | 2:2:2 |
4 | CT + CDHP + Glucose | 2:1:2 |
5 | Yeast Extract + PEPTONE + CS | 1:2:2 |
6 | Yeast Extract + CDHP + Glucose | 1:1:2 |
7 | CT + Peptone + Glucose | 2:2:2 |
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Sivapragasam, M.; Wilfred, C.D.; Jaganathan, J.R.; Krishnan, S.; Ab Wan Karim Ghani, W.A.W. Choline-Based Ionic Liquids as Media for the Growth of Saccharomyces cerevisiae. Processes 2019, 7, 471. https://doi.org/10.3390/pr7070471
Sivapragasam M, Wilfred CD, Jaganathan JR, Krishnan S, Ab Wan Karim Ghani WAW. Choline-Based Ionic Liquids as Media for the Growth of Saccharomyces cerevisiae. Processes. 2019; 7(7):471. https://doi.org/10.3390/pr7070471
Chicago/Turabian StyleSivapragasam, Magaret, Cecilia Devi Wilfred, Joshua Raj Jaganathan, Sooridarsan Krishnan, and Wan Azlina Wan Ab Wan Karim Ghani. 2019. "Choline-Based Ionic Liquids as Media for the Growth of Saccharomyces cerevisiae" Processes 7, no. 7: 471. https://doi.org/10.3390/pr7070471