Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Antibodies
2.2. Animals
2.3. CL316,243 Treatment
2.4. Blood Glucose Determination
2.5. Blood Lipids Detection
2.6. Dorsal Shell Temperature Detection
2.7. RNA Isolation and Real-Time PCR
2.8. Immunoblotting
2.9. H&E Staining
2.10. Immunohistochemistry Staining
2.11. Lipid Extraction
2.12. Lipidomics Analysis
2.13. Statistical Analysis
3. Results
3.1. Adrenergic Stimulation-Induced Browning Heterogeneity of White Adipose Tissue and Ameliorated Systemic Metabolism
3.2. Total Composition of Lipid Classes in VAT and SAT Altered in Response to CL316,243 Treatment
3.3. Exposure to CL316,243 Differentially Affected the Abundance of Glycerides in VAT and SAT
3.4. Adrenergic Stimulation Boosted the Abundance of More Glycerophospholipids Species in VAT Than in SAT
3.5. CL316,243 Increased Sphingolipids in VAT without Changes in SAT
3.6. Cardiolipin was Elevated by CL316,243 in both VAT and SAT
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lipid Species | VAT | SAT | ||||
---|---|---|---|---|---|---|
Fold Changes | P Value | Fold Changes | P Value | |||
68:6(16:1) | 20.52699194 | 0.000475 | *** | 1.766938316 | 0.079321 | |
68:5(16:1) | 13.97259484 | 0.000564 | *** | 2.008876544 | 0.026587 | # |
70:8(16:1) | 20.77862561 | 8.64 × 10−05 | *** | 2.005579532 | 0.10948 | |
70:7(16:1) | 11.95160231 | 0.001351 | ** | 1.979040735 | 0.016 | # |
70:6(16:1) | 14.10403798 | 0.000329 | *** | 2.406633843 | 0.035636 | # |
70:6(18:2) | 20.88860213 | 0.000393 | *** | 2.789402423 | 0.023778 | # |
70:5(16:1) | 12.5429712 | 0.000212 | *** | 2.560187318 | 0.053366 | |
70:5(18:2) | 14.13490498 | 1.74 × 10−05 | *** | 3.72660512 | 0.034428 | # |
70:4(16:1) | 12.17132084 | 0.001126 | ** | 3.984337045 | 0.004524 | ## |
70:4(18:2) | 12.61352092 | 7.52 × 10−05 | *** | 3.035547901 | 0.055842 | |
72:10(18:2) | 5.013887345 | 0.004586 | ** | 1.243596576 | 0.601292 | |
72:9(16:1) | 10.73074822 | 0.028529 | * | 0.678223578 | 0.437706 | |
72:9(18:2) | 18.59530648 | 7.67 × 10−06 | *** | 2.16986299 | 0.050667 | |
72:8(18:2) | 29.73292901 | 8.01 × 10−06 | *** | 2.441405669 | 0.025102 | # |
72:8(20:3) | 3.598494043 | 0.059739 | 1.292607526 | 0.554198 | ||
72:7(16:1) | 18.62111688 | 0.000183 | *** | 2.667047606 | 0.023761 | # |
72:7(18:2) | 30.45759488 | 1.79 × 10−06 | *** | 2.86873778 | 0.02882 | # |
72:6(16:1) | 2.799529192 | 0.046693 | * | 1.369963332 | 0.485846 | |
72:6(18:2) | 7.291839788 | 0.001115 | ** | 3.364376204 | 0.021878 | # |
72:5(18:2) | — | — | 3.561477455 | 0.024244 | # | |
74:11(16:1) | 2.745578422 | 0.053239 | 2.345654479 | 0.084162 | ||
74:11(18:2) | 2.139752181 | 0.134496 | 1.248345849 | 0.627876 | ||
74:10(18:2) | 11.78634294 | 0.000284 | *** | 1.790233115 | 0.080245 | |
74:10(20:4) | 8.968951403 | 0.000418 | *** | 1.193409387 | 0.752919 | |
74:9(18:2) | 11.71784353 | 0.000263 | *** | 1.512566398 | 0.23822 | |
74:9(20:3) | 5.513657707 | 0.00148 | ** | 1.035975783 | 0.909981 | |
74:8(18:2) | 8.34535855 | 0.000278 | *** | 1.663407978 | 0.171988 | |
74:8(20:3) | 3.029370298 | 0.078375 | 1.115185366 | 0.774192 | ||
76:13(18:2) | 6.755548969 | 0.000149 | *** | 2.524297408 | 0.045155 | # |
76:12(16:1) | 6.154661722 | 2.55 × 10−06 | *** | 3.278898397 | 0.003226 | ## |
76:12(18:2) | 4.323385873 | 0.000256 | *** | 2.217419145 | 0.035696 | # |
76:10(18:2) | 4.457581419 | 0.020536 | * | 1.521167675 | 0.226874 | |
76:10(20:3) | 0.449088362 | 0.556864 | 0.778169257 | 0.503334 | ||
76:9(18:2) | 2.536667231 | 0.07915 | 0.752584244 | 0.496683 | ||
78:13(18:2) | 2.141719426 | 0.12503 | 2.206003273 | 0.001864 | ## |
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He, P.; Hou, B.; Li, Y.; Xu, C.; Ma, P.; Lam, S.M.; Gil, V.; Yang, X.; Yang, X.; Zhang, L.; et al. Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation. Biomolecules 2019, 9, 444. https://doi.org/10.3390/biom9090444
He P, Hou B, Li Y, Xu C, Ma P, Lam SM, Gil V, Yang X, Yang X, Zhang L, et al. Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation. Biomolecules. 2019; 9(9):444. https://doi.org/10.3390/biom9090444
Chicago/Turabian StyleHe, Ping, Biyu Hou, Yanliang Li, Chunyang Xu, Peng Ma, Sin Man Lam, Victoria Gil, Xinyu Yang, Xiuying Yang, Li Zhang, and et al. 2019. "Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation" Biomolecules 9, no. 9: 444. https://doi.org/10.3390/biom9090444