MiR-191 as a Key Molecule in Aneurysmal Aortic Remodeling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Controls
2.2. Assessment of Circulating miR-191 Concentration
2.3. Cell Cultures
2.4. Transfection of Cultured Cells
2.5. RNA Isolation from Endothelial Cells
2.6. Library Construction and RNA-Sequencing
2.7. Bioinformatic Analysis of RNA-Seq Reads
2.8. Pathway Analysis
3. Results
3.1. Comparison of Circulating miR-191 between Patients and Controls
3.2. Differences in Gene Expression Profiles of Endothelial Cells after Transfection with miR-191
3.3. Comparison between Potential Targets of miR-191 and Differentially Expressed Genes
3.4. Pathway Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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AAA [n = 205] | Control Group [n = 180] | p Value | |||||
---|---|---|---|---|---|---|---|
Age [years, mean ± SD] | 68.98 | ± | 7.85 | 67.57 | ± | 7.32 | 0.3212 |
Male sex [n, %] | 170 | 82.93% | 160 | 80.00% | 0.4484 | ||
Hypertension [n, %] | 158 | 77.07% | 156 | 78.00% | 0.8232 | ||
Coronary artery disease [n, %] | 96 | 46.83% | 97 | 48.50% | 0.7367 | ||
Cardiac failure [n, %] | 21 | 10.24% | 32 | 16.00% | 0.8593 | ||
Aortic stenosis [n, %] | 7 | 3.41% | 6 | 3.00% | 0.4812 | ||
Deep vein thrombosis [n, %] | 6 | 2.93% | 7 | 3.50% | 0.7435 | ||
Pulmonary embolism [n, %] | 2 | 0.98% | 3 | 1.50% | 0.6327 | ||
Stroke [n, %] | 9 | 4.39% | 16 | 8.00% | 0.1312 | ||
TIA [n, %] | 2 | 0.98% | 2 | 1.00% | 0.9747 | ||
Obstructive sleep apnoea [n, %] | 7 | 3.41% | 6 | 3.00% | 0.8129 | ||
Gastric ulcer [n, %] | 4 | 1.95% | 2 | 1.00% | 0.4280 | ||
Analgetic therapy [n, %] | 11 | 5.37% | 15 | 7.50% | 0.3810 | ||
Nicotine [n, %] | 130 | 63.41% | 124 | 62.00% | 0.7680 | ||
Kidney failure [n, %] | 3 | 1.46% | 2 | 1.00% | 0.6730 |
AAA [n = 205] | |||
---|---|---|---|
Age [years, mean ± SD] | 68.98 | ± | 7.85 |
Age at the moment of diagnosis [years, mean ± SD] | 66.67 | ± | 8.45 |
Diameter of aneurysm [cm, mean ± SD] | 5.85 | ± | 1.43 |
Length of aneurysm [cm, mean ± SD] | 11.38 | ± | 3.99 |
Dissecting aneurysm [n, %] | 0 | 0.00% | |
Ruptured aneurysm [n, %] | 6 | 2.93% | |
Symptomatic aneurysm [n, %] | 48 | 23.41% |
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Lichołai, S.; Studzińska, D.; Plutecka, H.; Gubała, T.; Szczeklik, W.; Sanak, M. MiR-191 as a Key Molecule in Aneurysmal Aortic Remodeling. Biomolecules 2021, 11, 1611. https://doi.org/10.3390/biom11111611
Lichołai S, Studzińska D, Plutecka H, Gubała T, Szczeklik W, Sanak M. MiR-191 as a Key Molecule in Aneurysmal Aortic Remodeling. Biomolecules. 2021; 11(11):1611. https://doi.org/10.3390/biom11111611
Chicago/Turabian StyleLichołai, Sabina, Dorota Studzińska, Hanna Plutecka, Tomasz Gubała, Wojciech Szczeklik, and Marek Sanak. 2021. "MiR-191 as a Key Molecule in Aneurysmal Aortic Remodeling" Biomolecules 11, no. 11: 1611. https://doi.org/10.3390/biom11111611