Under Conditions of Amyloid Formation Bovine Carbonic Anhydrase B Undergoes Fragmentation by Acid Hydrolysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. BCAB Expression and Purification
2.2. BCAB Aggregation Conditions
2.3. Fluorescent Spectroscopy
2.4. Electron Microscopy
2.5. SDS-PAGE
2.6. Size-Exclusion Chromatography
2.7. Circular Dichroism (CD)
3. Results
3.1. BCAB Undergoes Hydrolysis under Conditions of Amyloid Formation
3.2. The Precursors of BCAB Amyloids Are Associated Peptides
3.3. Does Full-Length BCAB Form Aggregates?
4. Discussion
4.1. Precursor of BCAB Amyloids Is Protein Fragments
4.2. Acid Hydrolysis of Proteins: Features and the Need to Test
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Marchenkov, V.; Ryabova, N.; Balobanov, V.; Glukhov, A.; Ilyina, N.; Katina, N. Under Conditions of Amyloid Formation Bovine Carbonic Anhydrase B Undergoes Fragmentation by Acid Hydrolysis. Biomolecules 2021, 11, 1608. https://doi.org/10.3390/biom11111608
Marchenkov V, Ryabova N, Balobanov V, Glukhov A, Ilyina N, Katina N. Under Conditions of Amyloid Formation Bovine Carbonic Anhydrase B Undergoes Fragmentation by Acid Hydrolysis. Biomolecules. 2021; 11(11):1608. https://doi.org/10.3390/biom11111608
Chicago/Turabian StyleMarchenkov, Victor, Natalya Ryabova, Vitaly Balobanov, Anatoly Glukhov, Nelly Ilyina, and Natalya Katina. 2021. "Under Conditions of Amyloid Formation Bovine Carbonic Anhydrase B Undergoes Fragmentation by Acid Hydrolysis" Biomolecules 11, no. 11: 1608. https://doi.org/10.3390/biom11111608