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Article

Green Synthesis of Nitrogen–Doped Carbon Dots from Fresh Tea Leaves for Selective Fe3+ Ions Detection and Cellular Imaging

by
Guili Ge
1,
Lin Li
2,
Mingjian Chen
1,
Xu Wu
1,
Yuxin Yang
1,
Dan Wang
1,
Sicheng Zuo
1,
Zhaoyang Zeng
1,
Wei Xiong
1,* and
Can Guo
1,*
1
Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha 410008, China
2
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
*
Authors to whom correspondence should be addressed.
Nanomaterials 2022, 12(6), 986; https://doi.org/10.3390/nano12060986
Submission received: 14 February 2022 / Revised: 28 February 2022 / Accepted: 3 March 2022 / Published: 17 March 2022
(This article belongs to the Special Issue Advanced Carbon Chemistry and Its Applications)
Browse Figures
Versions Notes

Abstract

:
In this research, we successfully developed a green, economical and effective one–step hydrothermal method for the synthesis of fluorescent nitrogen–doped carbon dots (N–CDs) by utilizing fresh tea leaves and urea as the carbon and nitrogen sources, respectively. The obtained N–CDs were characterized by TEM, XPS and FT–IR. We found that the N–CDs were near–spherical with an average size of about 2.32 nm, and contained abundant oxygen and nitrogen functional groups. The N–CDs exhibited bright blue fluorescence under ultraviolet illumination, with the maximum emission at 455 nm. Meanwhile, the as–prepared N–CDs could be selectively quenched by Fe3+ ions. The quenching of N–CDs is linearly correlated with the concentration of Fe3+ in the range of 0.1–400 μM with a low detection limit of 0.079 μM. Significantly, the N–CDs present excellent biocompatibility and high photostability. The results also depict that multicolor fluorescence is displayed under a fluorescence microscope and successfully applied for the detection of intracellular Fe3+. To sum up, the fluorescent N–CDs are expected to be a sensitive detection probe for Fe3+ in biological systems.

1. Introduction

Carbon dots (CDs), are novel fluorescent carbon nanomaterials with a particle size of less than 10 nm. Since their accidental discovery in 2004 [1], they have attracted more and more attention because of their unique performance advantages and potential in biological imaging, biosensors, drug delivery and theranostics. Compared with traditional organic fluorescent dyes and inorganic quantum dots, CDs have good biocompatibility, water dispersion, photostability, environmental protection qualities, low cost, adjustable emission, easy modifiability and other unique properties. A variety of methods are being explored to prepare CDs; these methods are generally classified into two categories: “top–down” and “bottom–up” methods [2]. Top–down methods cut large size carbon precursors into small size carbon nanodots by physical or chemical methods, including arc discharge, laser etching, electrochemical oxidation and chemical oxidation. These methods are often limited by the high cost of equipment, complex conditions and high cost. The bottom–up method uses small molecules as precursors to obtain larger CDs through a series of chemical reactions, including the template method, microwave method, pyrolysis method and solvothermal/hydrothermal method. Among these methods, the hydrothermal method is considered a simple, green and effective synthesis method, and has become a common method for the preparation of CDs. The carbon sources of the hydrothermal method are very rich; natural products (such as milk [3,4], bark [5], juice [6,7], leaves [8], food residue [9] or biological waste [10,11,12]) or artificial materials (such as citric acid [13,14], glucose [15,16,17], protein [18] or phenylenediamine [19,20]) all can be used as raw materials to synthesize CDs. Because CDs in biomedical applications mostly require high fluorescence, good water solubility and low toxicity, natural products are more popular. However, most as–prepared CDs have low yield and poor fluorescence. To improve the fluorescence properties of CDs, surface passivation and heteroatom doping are often used [21]. However, surface passivation is complex and time–consuming and doping metal atoms has high cost and high toxicity, while doping nonmetallic elements (such as nitrogen) becomes an economical and effective method due to its low cost and low toxicity [22]. Although the fluorescence emission mechanism of CDs has not been fully elucidated, we know that nitrogen atoms have a similar atomic size and five valence electrons available for bonding with carbon atoms, effectively regulating the inherent properties of CDs and improving fluorescence [23]. Therefore, it is of great significance to find suitable natural precursors for the preparation of high–fluorescence nitrogen–doped CDs (N–CDs) to improve the application value of CDs in biomedicine.
Fe3+ is one of the necessary transition metal cations in the human body. It plays an important role in biological and environmental systems, and plays a crucial role in many physiological and pathological processes such as cell metabolism, enzyme catalysis, energy transport, DNA and RNA synthesis and repair [24,25,26]. Both high and low levels of Fe3+ can cause an imbalance of cellular homeostasis, leading to various diseases, such as anemia, liver and kidney dysfunction, heart failure, diabetes, Alzheimer’s disease, Parkinson’s disease and cancer [27]. Additionally, an excess of iron ion (Fe3+) in living cells can be generated by Fenton reaction catalytic reactive oxygen species, which can damage lipids, nucleic acids and proteins [28]. Studies have shown that there is a clear relationship between cell ferroptosis and iron excess [29,30]. Therefore, the determination of Fe3+ content is of great significance for the early clinical diagnosis of these diseases. At present, there have been several methods for determination of Fe3+, but the complicated procedure and high cost limit its practical application [24,31]. Fluorescence sensing platform based on fluorescence spectrum quenching technology is widely needed due to its simplicity, low cost and high sensitivity [32], and fluorescent carbon dots have become an important choice due to their unique advantages. Natural carbon sources have more obvious advantages: easy obtainment, environmental protection, low cost, and since biomedical application of CDs mostly requires high fluorescence, good water solubility and low toxicity. CDs obtained from natural products are widely used in biosensors and biological imaging. For instance, Yu et al. reported that fluorescent CDs extracted from Jinhua bergamot was used as a Fe3+ probe with a detection limit of 0.075 μM [33]. Song et al. prepared fluorescent CDs using black tea, achieving the detection limit of Fe3+ 0.25 μM [34]. Yu et al. used the CDs prepared from red lentils as Fe3+ with a detection limit of 0.10 μM [35]. Although some progress has been made in the detection of Fe3+ by CDs, some of these studies remain extracellular or the detection limit is not low enough. Therefore, it is very urgent to develop a green economy precursor with high fluorescence carbon dots to realize the highly sensitive detection of Fe3+ in cells.
In this research, we developed a very simple and ultra–green method for preparing N–CDs; we prepared high–fluorescence N–CDs using fresh tea and urea as raw materials by one–pot hydrothermal method (Scheme 1). The fresh tea has been cultivated in a large area in Hunan (China). As a natural raw material, it has no processing and low cost. This is not only environmentally friendly, but also contains a lot of catechin, folic acid, pantothenic acid and other ingredients, and can improve human health. The prepared N–CDs show good water solubility, stable fluorescence and excellent compatibility, and the cytotoxicity to human cells was negligible. N–CDs can respond to Fe3+ with high sensitivity and can be used as an effective fluorescent probe to selectively detect Fe3+ by fluorescence quenching behavior. In addition, different from the previously reported CDs from tea, the N–CDs were evaluated as fluorescent probes for the intracellular multicolor imaging and sensing of Fe3+, achieving the detection of Fe3+ at the cellular level.

2. Experimental Section

2.1. Materials

Fresh tea leaves were collected from Hunan Agricultural University. AlCl3, Co(NO3)2, CuSO4, FeCl3, GdCl2, KCl, MgCl2, MnCl2, NaCl, Ni(NO3)2, AgNO3, CaCl2 and ZnSO4 were acquired from Sinopharm Chemical Reagent. All reagents were of analytical reagent grade and used without further purification. RPMI–640 culture medium, 10% Fetal Bovine Serum (FBS), 100 U/mL penicillin and 100 μg·mL−1 streptomycin were purchased from Gibco. Ethylenediaminetetraacetic acid (EDTA), cell counting kit–8 (CCK–8) were purchased from APExBIO. Ultrapure distilled water was prepared through a Millipore system and used in whole experiments.

2.2. Synthesis of N–CDs

The N–CDs were prepared by a one–pot hydrothermal method using fresh tea. Firstly, fresh tea leaves were dried in the electric oven and then ground into small pieces. Next, 5 g urea was dissolved in 100 mL ultrapure distilled water and 5 g tea fragments were added. The mixture was transferred into a 150 mL Teflon–lined stainless–steel autoclave. After heating at 200 °C for 10 h and cooling down naturally to room temperature, dark–brownish solutions were formed. The product was centrifuged (10,000 rpm, 20 min), and further purified into a supernate via a dialysis membrane (Cut off = 500 Da) for 2 days with ultrapure water. Finally, the dark brown powders were collected by drying under vacuum and stored at 4 °C for further studies.

2.3. Characterizations

Fluorescence spectra were measured on a RF–6000 spectrophotometer (Shimadzu, Tokyo, Japan). The ultraviolet–visible (UV–Vis) absorption spectrum was measured on a UV–2600 spectrophotometer (Shimadzu, Japan). Transmission electron microscopy (TEM) pictures were acquired from the JEM–2100F (JEOL, Tokyo, Japan). X-ray photoelectron spectroscopy (XPS) analysis was carried out with an EscaLab 250Xi (Thermo Fisher, Waltham, MA, USA). Fourier transform infrared spectroscopy (FTIR) spectra were recorded on a Nicolet 6700 (Thermo Fisher, Waltham, MA, USA). The zeta potential measurements were finished with a Malvern Nano ZS90 instrument. All pH values were measured by a FiveEasy Plus FE28 (METTLER TOLEDO, Shanghai, China). The fluorescence imaging was observed with a fluorescence microscope (BX51, Olympus, Tokyo, Japan) with a 40× objective lens.

2.4. Sensitivity and Selectivity Detection of Fe3+ Ions

To evaluate the sensitivity towards Fe3+, 500 μL of 100 μg·mL−1 N–CDs solution was mixed with different amounts of 10 mM Fe3+ ions, so that the final concentration of N–CDs was 50 μg·mL−1 (the optimization of the N–CDs concentration was shown in Figure S1, Supporting Information) and the concentration of Fe3+ was 0 to 1 mM, and the volume was raised to 1 mL by adding water. The mixture solution was maintained for 10 min at room temperature; all of the fluorescent emission spectra were recorded under the excitation wavelength of 360 nm. The limit of lower detection (LOD) was calculated based on the following equation.
LOD = 3σ/K
where K is the slope for the range of the linearity, σ is the standard deviation of the blank (n = 11).
The selectivity of N–CDs was detected by the interfered metal ions such as Al3+, Co2+, Cu2+, Gd2+, K+, Mg2+, Mn2+, Na+, Ni2+, Ag+, Ca2+ and Zn2+ ions according to steps similar to those depicted above. The fluorescence intensity of all these solutions was recorded at a 360 nm excitation wavelength. Relative fluorescence intensity (F/F0) was calculated on addition with different metal ions, where F0 and F are the fluorescence intensity of the N–CD solution in the absence and presence of different metal ions, respectively.

2.5. pH Stable Fluorescence Properties

A quantity of 200 μL prepared N–CDs solution (1 mg·mL−1) was taken and mix with 3800 μL hepes solution. A small amount of strong alkali NaOH and strong acid HCl solution was added to adjust the pH of the mixture solution to 1, 3, 5, 7, 9, 11 and 13. The fluorescence properties at various pH values were evaluated by fluorescence spectroscopy.

2.6. Cytotoxicity Assay

The cytotoxicity of N–CDs was evaluated by CCK–8 assay, and A549 cells were selected as model cells to value the biocompatibility of N–CDs. Firstly, the cells were cultured in RPMI–1640 media supplemented with 10% heat–inactivated fetal bovine serum. The cells were seeded at a density of 1 × 104 cells per well into 96–well plates and incubated for 24 h at 37 °C with 5% CO2. After that, the cells plates were added in different concentrations of N–CD solutions (0, 25, 50, 100, 200, 400 μg·mL−1) and cultured for 24 h. Following this, 10 μL of CCK–8 solution (dissolved in 100 uL serum–free culture) was introduced to each well under dark conditions, then culturing was continued for 2 h in 37 °C. Finally, the absorbance was measured at 450 nm with a microplate reader and cell viability was calculated.

2.7. Cellular Imaging

The A549 cells were seeded into a 24–well plate in which circular glass slides were placed, and then cultured in 5% CO2 atmosphere at 37 °C for 24 h. Next, 50 μL of 1 mg·mL−1 N–CDs solution mixed with 450 μL RPMI–1640 medium was added into the dish containing cells, and further cultured for 6 h at 37 °C. The culture medium was discarded and washed three times with PBS buffer (pH = 7.4). Instead, 10 μL of 50 mM Fe3+ was added to the cells and incubated for further 1 h. The glass slides were taken out and, after washing three times with PBS, the cells were fixed with 4% paraformaldehyde for 30 min. Lastly, a fluorescence microscope was used to observe the cell imaging under a 40× eyepiece.

3. Results and Discussion

3.1. Structural Characterizations of N–CDs

Aiming at obtaining highly fluorescent CDs by introducing nitrogen atoms, we synthesized a kind of water–soluble N–CD by a facile one–pot hydrothermal method with fresh tea as carbon source and urea as nitrogen source, respectively. After filtrating, centrifugating, dialysing and drying, well monodispersed N–CDs were obtained. Subsequently, we characterized the N–CDs by TEM, XPS and FTIR. The morphology and structure of the prepared N–CDs were characterized by TEM. As shown in Figure 1A, the N–CDs were near–spherical and monodispersed in an aqueous solution without apparent aggregation, which is consistent with other CDs reported in the previous literature [36,37]. The corresponding size–distribution histogram revealed that the average size of the N–CDs mainly ranged from 2.0 to 2.5 nm and the average diameter was 2.32 nm (Figure 1B). The nanoscale small size and good water solubility lay a good foundation for the effective uptake of N–CDs in cells.
The functional groups and element composition of the N–CDs were determined by XPS. The full–survey XPS was depicted in Figure 2A, revealing that the three peaks of C1s, N1s and O1s at 285.08 eV, 399.08 eV and 532.08 eV, respectively, indicating that the N–CDs are primarily composed of three types of elements including carbon (65.49 at%), nitrogen (6.02 at%), oxygen (28.49 at%). The successful doping of the N atom is proved. Further high resolution spectra of C1S in Figure 2B gathered six peaks at 288.49, 287.57, 286.75, 285.79, 284.83 and 284.23 eV, which were assigned to O–C=O, C=O, C–O, C–N, C=C and C–C, respectively [38,39]. The three peaks at 401.39, 399.40 and 397.68 eV in the N 1s specctrum in Figure 2C were attributed to the graphitic N, pyrrolic N and pyridinic N, respectively [40]. The above analysis demonstrates that the N–CDs are have rich oxygen and nitrogen functional groups, which is consistent with the typical characteristics of N–CDs. The Fourier transform infrared (FI–IR) spectra further confirmed the surface functional groups of the obtained N–CDs. As illustrated in Figure 2D, the characteristic absorption peak around 3362 cm−1 is assigned to stretching vibrations of N–H and –OH bonds [39]. The peak at 2932 cm−1 is related to stretching of C–H [39]. The peak at 1640 cm−1 is ascribed to the bending vibration of N–H and the stretching vibration of the double bond of C=C [24], whereas the peak occured at 1402 cm−1 could be associated to the stretching vibration of CH2/C–N [41]. Furthermore, due to C–O extension, an absorption peaked at 1038 cm−1 is observed [41]. Thus, the surface of the synthesized N–CDs is also full of functional groups of carboxyl, hydroxyl and amino, which are in good agreement with the results of XPS measurement. Furthermore, the zeta potential of the N–CDs was measured to investigate the functional groups with charges on the surface of N–CDs; the value in ultrapure water was −13.80 mV, indicating that there were more oxygen–containing functional groups such as negatively charged carboxyl groups and hydroxyl groups than positively charged amino groups on the surface of N–CDs [42,43]. The existence of different hydrophilic functional groups on the surface of the N–CDs means N–CDs have good water solubility and biological application potential.

3.2. Optical Properties of N–CDs

The UV–vis absorption and fluorescence spectra were investigated to evaluate the optical properties of the as–prepared N–CDs. As shown in Figure 3A, the absorption peak at 280 nm, which is mainly derived from the electron π–π* transition of C=C bonds [44]. The strongest emission intensity at approximately 455 nm is obtained with an excitation at 360 nm. In addition, we can also observe that the N–CD aqueous solutions are dark brown, clear and transparent under daylight, and yet exhibit a bright blue fluorescence under UV light (365 nm) from the top–right inset of Figure 3A. We can confirm that the obtained N–CDs have excellent fluorescence properties and are closely related to the doping of N atoms. In order to get a better understanding of the properties, further measurement was made; the emission spectra of N–CDs were measured at various excitation wavelengths ranging from 340 nm to 500 nm, and the result is shown in Figure 3B. It is noticed that emission peaks of N–CDs are gradually red–shifted as the excitation wavelength increases, which provides a foundation for N–CDs to realize intracellular multicolor imaging. Fluorescence properties of N–CDs in sodium chloride solution were measured to eliminate the interference of chloride ions, as shown in Figure 3C. When the concentration of sodium chloride increases from 10−4 M to 1.0 M, the fluorescence emission intensity of N–CDs changes little, indicating that the prepared N–CDs can be protected from the effects of high ionic strength. Figure 3D exhibits that fluorescent spectra of the N–CDs at different pH values. It is noteworthy that the fluorescence emission peak of the N–CDs is red–shifted to some extent as pH value increases from 1 to 13, which might be related to the functional groups on the N–CD surfaces [10]. More importantly, the fluorescence intensity of the N–CDs increases along with increasing pH up to 6 and then decreases. The optimal pH to gain maximum fluorescence intensity observed for the N–CDs is at pH ≈ 6, indicating that the fluorescence properties of the N–CDs are closely related to pH value. These may be attributed to the presence of phenols (–OH) and carbonyl groups (C=O) on the surface of N–CDs. These behaviors indicate that the fluorescence of N–CDs could be quenching in strong acid and alkali environments, yet in contrary in near neutral environment, are conducive to the application of N–CDs in metal ion sensing and biological imaging [42]. Additionally, the stability of the fluorescence of N–CDs was evaluated, as displayed in Figure S2 (Supporting Information). The results show that the fluorescence intensity did not change significantly after the N–CD aqueous solution was left for one month. Since aggregation is one of the most common problems for application of nanomaterials in biomedical fields, the stability of N–CD dispersion in water, phosphate–buffered saline (PBS) and RPMI–1640 cell culture medium (containing serum) was evaluated and no aggregation was found to occur even after standing for 30 days (Figure S3, Supporting Information).

3.3. Fluorescence Response of N–CDs toward Fe3+

We conducted a preliminary study on the feasibility of the synthesized N–CDs as a Fe3+ sensor, and the results are shown in Figure 4A. As can be seen from the black curve, when the excitation wavelength was 360 nm, N–CDs showed a strong fluorescence signal (corresponding to the left illustration), indicating that N–CDs have fluorescence properties, while Fe3+ solution, as reported in the literature [34] does not show fluorescence properties (blue curve, corresponding to the right illustration). Surprisingly, when 1 mM Fe3+ was added to the N–CD solution, the fluorescence signal was significantly reduced in the red curve (corresponding to the middle illustration), indicating that Fe3+ did have fluorescence quenching effect on N–CDs. This indicated that the N–CD method is feasible to detect Fe3+. In Figure 4B, the absorption peak of N–CDs was significantly enhanced in the presence of Fe3+, and a new absorption band appeared at about 290 nm, which was consistent with that of Fe3+ ions, which shows that the absorption band is caused by the complexation of Fe3+. The quenching may be caused by effective coordination or chelation between Fe3+ ions and some groups on the surface of N–CDs. The previous analysis of XPS and FTIR data showed that N–CDs contains abundant carboxyl, hydroxyl and amino functional groups, which can act as the electron donors, while Fe3+ has a half–filled 3d orbital, which can play as electron acceptor. This results in effective coordination interaction between Fe3+ and the N–CD solution, which breaks the radiative jump, and causes the excited electrons of N–CDs to transfer to the three–dimensional orbit of Fe3+, resulting in fluorescence quenching [23,35,45,46]. Thus, we speculated on the possible mechanism of N–CD fluorescence quenching by Fe3+, as shown in Figure 4C.

3.4. Fluorescent Sensing for Fe3+

In order to study the sensitivity of the prepared fluorescent N–CDs towards the detection of Fe3+ ions, the fluorescence emission spectra of N–CDs aqueous solution with the addition of different concentrations of Fe3+ at an excitation wavelength of 360 nm were measured. The concentration of Fe3+ ranged from 0 to 1000 μM. It was noted from Figure 5A that the maximum fluorescence emission intensity of the N–CDs gradually decreased as the concentration of Fe3+ increased, indicating that the fluorescence quenching that occurred should be ascribed to the addition of Fe3+ ions. Figure 5B exhibits the relationship between maximum fluorescence intensity with the concentration of Fe3+ ions. It could be found that 80% fluorescence of N–CDs could be quenched at 1 mM concentration of Fe3+, indicating that the obtained N–CDs could be used for quantitative detection of Fe3+ ions. Figure 5C shows a good linear correlation (R2 = 0.9948) in a wide range from 0.1 μM to 400 μM and the detection limit was calculated to be 0.079 μM, indicating that the N–CDs can be used as a sensing probe for Fe3+ ions. Table 1 lists the sensing performance of different CD–based fluorescent probes for Fe3+ detection. Compared with many other CDs for the detection of Fe3+ reported in previous literature, our N–CDs probe has many advantages, such as higher sensitivity (rapid response), relatively wider linear range (0.1–400 μM) and lower detection limit (0.079 μM). More importantly, the N–CD probe has more advantages. Our probe preparation method is simple, efficient, made from abundant raw materials, low cost and environmentally friendly. For the sake of further exploring the selectivity of the N–CDs as an bio–sensor for Fe3+, we evaluated the change in fluorescence intensity of N–CDs after adding different metal ions, including Al3+, Co2+, Cu2+, Fe3+, Gd2+, K+, Mg2+, Mn2+, Na+, Ni2+, Ag+, Ca2+ and Zn2+ ions. After the metal ions were added to the N–CD aqueous solution, it was left standing for 10 min, the final concentration of all the metal ion solution was corresponding (1 mM). The fluorescence intensity of the mixture measured under the excitation wavelength of 360 nm was recorded. The relative fluorescence intensity (F/F0) was shown in Figure 5D; it was observed that there was only a slight and negligible change in emission intensity of the N–CDs on adding various metal ions except Fe3+ ions, while the addition of Fe3+ ion exhibited noticeable quenching. This indicates that Fe3+ has a stronger affinity with N–CDs than other metal ions. N–CDs can be used as a specific probe for the detection of Fe3+.

3.5. Cytotoxicity and Cell Imaging

N–CDs have a series of attractive characteristics, such as their small size, fluorescence stability and good biocompatibility, and have great application potential in biosensors, biological imaging, drug delivery and other fields. In order to evaluate the application potential of N–CDs in cell imaging, it is necessary to evaluate their cytotoxicity. Firstly, we determined the effect of N–CDs on the cell viability of A549 cells by CCK–8 assay. When N–CDs of different concentrations were co–incubated with A549 cells for 24 h, the cell viability results were shown in Figure 6. As can be seen, with the gradual increasing of N–CD concentration, the cell viability almost did not change significantly. Even when the concentration of N–CDs reached 400 μg·mL−1, the cell viability was still above 85%, indicating that N–CDs had very low cytotoxicity, which lays a good foundation for N–CDs’ application in cell imaging and other biological applications. The low toxicity of the as–prepared N–CDs enables them to be used as a fluorescent probe in cell imaging. Next, A549 cells were incubated with RPMI–1640 culture containing 100 μg·mL−1 N–CDs for 6 h. The images were obtained under bright field and two different excitation light sources (405 nm, and 488 nm). As shown in Figure 7, there was no significant change or damage in cell morphology after N–CDs treatment. Under the excitation of different wavelengths, blue and green fluorescence appeared in the cytoplasm of the cells, respectively, indicating that N–CDs could be effectively absorbed by A549 cells after 6 h of culture, and their fluorescence characteristics remained unchanged in the cells (Figure 7A–C). However, fluorescence was not observed in N–CDs and Fe3+ co–incubated cells (Figure 7D–F), which is due to the extra Fe3+ entering the cell quenching the fluorescence of N–CDs. Compared with the CDs prepared by the green pathway reported in most literature, the N–CDs could be able to display multicolor fluorescence in cell imaging. Therefore, this illustrates that CDs extracted from natural products and synthesized by this green method exhibit low toxicity, good cell biocompatibility and cell imaging ability.

4. Conclusions

In this study, N–doped high–fluorescence CDs were successfully prepared by a one–step hydrothermal approach using fresh tea leaves as a carbon source and urea as a nitrogen source, respectively. The preparation method was simple, effective, green, economic and environmentally friendly. The N–CDs exhibited obvious excitation wave–dependent fluorescence, bright blue fluorescence under ultraviolet light and good stability in long–term storage. The fluorescence of N–CDs can be spontaneously quenched by Fe3+ ions, and can be used as a fluorescence probe for Fe3+ ions with high sensitivity and good selectivity with a detection limit as low as 0.079 μM. At the same time, N–CDs were proved to show very low cytotoxicity and good biocompatibility at the cellular level, and can also realize multicolor imaging and intracellular Fe3+ sensing. Therefore, the development of green economic raw materials to prepare N–CDs is a very promising production strategy, and provides an effective method for the successful application of multicolor imaging and iron level detection in vivo.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/nano12060986/s1, Figure S1: Optimization of the concentration of N–CDs; Figure S2: Fluorescent stability of the N–CDs; Figure S3: The photos of N–CDs dispersed in H2O, PBS and 1640 cell culture medium during 30 days.

Author Contributions

Conceptualization, G.G., L.L. and X.W.; methodology, G.G. and M.C.; software, G.G. and Y.Y.; validation, G.G. and S.Z.; formal analysis, G.G. and D.W.; investigation, M.C., S.Z. and D.W.; resources, X.W., C.G., W.X. and Z.Z.; data curation, X.W., C.G., W.X. and Z.Z.; writing—original draft preparation, G.G. and L.L.; writing—review and editing, G.G., L.L. and M.C.; visualization, G.G. and S.Z.; supervision, X.W., C.G., W.X. and Z.Z.; project administration, C.G., W.X.; funding acquisition, X.W., C.G., W.X. and Z.Z. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China [82072374, and U21A20382] and the Fundamental Research Funds for Central Universities of the Central South University [2021zzts0937].

Data Availability Statement

All data are available upon request.

Conflicts of Interest

There are no conflicts to declare.

References

  1. Xu, X.Y.; Ray, R.; Gu, Y.L.; Ploehn, H.J.; Gearheart, L.; Raker, K.; Scrivens, W.A. Electrophoretic analysis and purification of fluorescent single–walled carbon nanotube fragments. J. Am. Chem. Soc. 2004, 126, 12736–12737. [Google Scholar] [CrossRef] [PubMed]
  2. Jaleel, J.A.; Pramod, K. Artful and multifaceted applications of carbon dot in biomedicine. J. Control. Release 2018, 269, 302–321. [Google Scholar] [CrossRef] [PubMed]
  3. Wang, D.; Zhu, L.; McCleese, C.; Burda, C.; Chen, J.-F.; Dai, L. Fluorescent carbon dots from milk by microwave cooking. RSC Adv. 2016, 6, 41516–41521. [Google Scholar] [CrossRef]
  4. Wang, L.; Zhou, H.S. Green Synthesis of Luminescent Nitrogen–Doped Carbon Dots from Milk and Its Imaging Application. Anal. Chem. 2014, 86, 8902–8905. [Google Scholar] [CrossRef] [PubMed]
  5. Qin, X.; Lu, W.; Asiri, A.M.; Al-Youbi, A.O.; Sun, X. Green, low–cost synthesis of photoluminescent carbon dots by hydrothermal treatment of willow bark and their application as an effective photocatalyst for fabricating Au nanoparticles–reduced graphene oxide nanocomposites for glucose detection. Catal. Sci. Technol. 2013, 3, 1027–1035. [Google Scholar] [CrossRef]
  6. Sahu, S.; Behera, B.; Maiti, T.K.; Mohapatra, S. Simple one–step synthesis of highly luminescent carbon dots from orange juice: Application as excellent bio–imaging agents. Chem. Commun. 2012, 48, 8835–8837. [Google Scholar] [CrossRef]
  7. De, B.; Karak, N. A green and facile approach for the synthesis of water soluble fluorescent carbon dots from banana juice. RSC Adv. 2013, 3, 8286–8290. [Google Scholar] [CrossRef]
  8. Hu, Z.; Jiao, X.-Y.; Xu, L. The N,S co–doped carbon dots with excellent luminescent properties from green tea leaf residue and its sensing of gefitinib. Microchem. J. 2020, 154, 104588. [Google Scholar] [CrossRef]
  9. Hu, Y.; Yang, J.; Tian, J.; Jia, L.; Yu, J.-S. Waste frying oil as a precursor for one–step synthesis of sulfur–doped carbon dots with pH–sensitive photoluminescence. Carbon 2014, 77, 775–782. [Google Scholar] [CrossRef]
  10. Shi, Y.X.; Liu, X.; Wang, M.; Huang, J.B.; Jiang, X.Q.; Pang, J.H.; Xu, F.; Zhang, X.M. Synthesis of N–doped carbon quantum dots from bio–waste lignin for selective irons detection and cellular imaging. Int. J. Biol. Macromol. 2019, 128, 537–545. [Google Scholar] [CrossRef]
  11. Kang, C.; Huang, Y.; Yang, H.; Yan, X.F.; Chen, Z.P. A Review of Carbon Dots Produced from Biomass Wastes. Nanomaterials 2020, 10, 2316. [Google Scholar] [CrossRef] [PubMed]
  12. Kurian, M.; Paul, A. Recent trends in the use of green sources for carbon dot synthesis–A short review. Carbon Trends 2021, 3, 100032. [Google Scholar] [CrossRef]
  13. Zhang, Y.; Hu, L.; Sun, Y.; Zhu, C.; Li, R.; Liu, N.; Huang, H.; Liu, Y.; Huang, C.; Kang, Z. One–step synthesis of chiral carbon quantum dots and their enantioselective recognition. RSC Adv. 2016, 6, 59956–59960. [Google Scholar] [CrossRef]
  14. Ludmerczki, R.; Mura, S.; Carbonaro, C.M.; Mandityk, I.M.; Carraro, M.; Senes, N.; Garroni, S.; Granozzi, G.; Calvillo, L.; Marras, S.; et al. Carbon Dots from Citric Acid and its Intermediates Formed by Thermal Decomposition. Chem. Eur. J. 2019, 25, 11963–11974. [Google Scholar] [CrossRef] [PubMed]
  15. Ma, Z.; Ming, H.; Huang, H.; Liu, Y.; Kang, Z. One–step ultrasonic synthesis of fluorescent N–doped carbon dots from glucose and their visible–light sensitive photocatalytic ability. New J. Chem. 2012, 36, 861–864. [Google Scholar] [CrossRef]
  16. Yang, Z.-C.; Wang, M.; Yong, A.M.; Wong, S.Y.; Zhang, X.-H.; Tan, H.; Chang, A.Y.; Li, X.; Wang, J. Intrinsically fluorescent carbon dots with tunable emission derived from hydrothermal treatment of glucose in the presence of monopotassium phosphate. Chem. Commun. 2011, 47, 11615–11617. [Google Scholar] [CrossRef]
  17. Dou, Q.; Fang, X.; Jiang, S.; Chee, P.L.; Lee, T.-C.; Loh, X.J. Multi–functional fluorescent carbon dots with antibacterial and gene delivery properties. RSC Adv. 2015, 5, 46817–46822. [Google Scholar] [CrossRef]
  18. Zhang, Z.; Hao, J.; Zhang, J.; Zhang, B.; Tang, J. Protein as the source for synthesizing fluorescent carbon dots by a one–pot hydrothermal route. RSC Adv. 2012, 2, 8599–8601. [Google Scholar] [CrossRef]
  19. Vedamalai, M.; Periasamy, A.P.; Wang, C.W.; Tseng, Y.T.; Ho, L.C.; Shih, C.C.; Chang, H.T. Carbon nanodots prepared from o–phenylenediamine for sensing of Cu(2+) ions in cells. Nanoscale 2014, 6, 13119–13125. [Google Scholar] [CrossRef]
  20. Li, J.; Zuo, G.; Pan, X.; Wei, W.; Qi, X.; Su, T.; Dong, W. Nitrogen–doped carbon dots as a fluorescent probe for the highly sensitive detection of Ag(+) and cell imaging. Luminescence 2018, 33, 243–248. [Google Scholar] [CrossRef]
  21. Bai, L.; Yan, H.; Feng, Y.; Feng, W.; Yuan, L. Multi–excitation and single color emission carbon dots doped with silicon and nitrogen: Synthesis, emission mechanism, Fe3+ probe and cell imaging. Chem. Eng. J. 2019, 373, 963–972. [Google Scholar] [CrossRef]
  22. Huang, S.; Yang, E.; Yao, J.; Liu, Y.; Xiao, Q. Red emission nitrogen, boron, sulfur co–doped carbon dots for “on–off–on” fluorescent mode detection of Ag+ ions and l–cysteine in complex biological fluids and living cells. Anal. Chim. Acta 2018, 1035, 192–202. [Google Scholar] [CrossRef] [PubMed]
  23. Liu, W.; Diao, H.; Chang, H.; Wang, H.; Li, T.; Wei, W. Green synthesis of carbon dots from rose–heart radish and application for Fe3+ detection and cell imaging. Sens. Actuators B Chem. 2017, 241, 190–198. [Google Scholar] [CrossRef]
  24. Sun, X.; He, J.; Yang, S.; Zheng, M.; Wang, Y.; Ma, S.; Zheng, H. Green synthesis of carbon dots originated from Lycii Fructus for effective fluorescent sensing of ferric ion and multicolor cell imaging. J. Photochem. Photobiol. B 2017, 175, 219–225. [Google Scholar] [CrossRef] [PubMed]
  25. Li, S.; Li, Y.; Cao, J.; Zhu, J.; Fan, L.; Li, X. Sulfur–Doped Graphene Quantum Dots as a Novel Fluorescent Probe for Highly Selective and Sensitive Detection of Fe3+. Anal. Chem. 2014, 86, 10201–10207. [Google Scholar] [CrossRef]
  26. Shangguan, J.; Huang, J.; He, D.; He, X.; Wang, K.; Ye, R.; Yang, X.; Qing, T.; Tang, J. Highly Fe3+–Selective Fluorescent Nanoprobe Based on Ultrabright N/P Codoped Carbon Dots and Its Application in Biological Samples. Anal. Chem. 2017, 89, 7477–7484. [Google Scholar] [CrossRef] [PubMed]
  27. Torti, S.V.; Manz, D.H.; Paul, B.T.; Blanchette-Farra, N.; Torti, F.M. Iron and Cancer. Annu. Rev. Nutr. 2018, 38, 97–125. [Google Scholar] [CrossRef]
  28. Hamishehkar, H.; Ghasemzadeh, B.; Naseri, A.; Salehi, R.; Rasoulzadeh, F. Carbon dots preparation as a fluorescent sensing platform for highly efficient detection of Fe(III) ions in biological systems. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2015, 150, 934–939. [Google Scholar] [CrossRef]
  29. Liu, T.; Liu, W.; Zhang, M.; Yu, W.; Gao, F.; Li, C.; Wang, S.-B.; Feng, J.; Zhang, X.-Z. Ferrous–Supply–Regeneration Nanoengineering for Cancer–Cell–Specific Ferroptosis in Combination with Imaging–Guided Photodynamic Therapy. ACS Nano 2018, 12, 12181–12192. [Google Scholar] [CrossRef]
  30. Tang, H.; Chen, D.; Li, C.; Zheng, C.; Wu, X.; Zhang, Y.; Song, Q.; Fei, W. Dual GSH–exhausting sorafenib loaded manganese–silica nanodrugs for inducing the ferroptosis of hepatocellular carcinoma cells. Int. J. Pharm. 2019, 572, 118782. [Google Scholar] [CrossRef]
  31. Arnold, G.L.; Weyer, S.; Anbar, A.D. Fe Isotope Variations in Natural Materials Measured Using High Mass Resolution Multiple Collector ICPMS. Anal. Chem. 2004, 76, 322–327. [Google Scholar] [CrossRef] [PubMed]
  32. Lu, W.; Gong, X.; Nan, M.; Liu, Y.; Shuang, S.; Dong, C. Comparative study for N and S doped carbon dots: Synthesis, characterization and applications for Fe(3+) probe and cellular imaging. Anal. Chim. Acta 2015, 898, 116–127. [Google Scholar] [CrossRef] [PubMed]
  33. Yu, J.; Song, N.; Zhang, Y.-K.; Zhong, S.-X.; Wang, A.-J.; Chen, J. Green preparation of carbon dots by Jinhua bergamot for sensitive and selective fluorescent detection of Hg2+ and Fe3+. Sens. Actuators B Chem. 2015, 214, 29–35. [Google Scholar] [CrossRef]
  34. Song, P.; Zhang, L.; Long, H.; Meng, M.; Liu, T.; Yin, Y.; Xi, R. A multianalyte fluorescent carbon dots sensing system constructed based on specific recognition of Fe(iii) ions. RSC Adv. 2017, 7, 28637–28646. [Google Scholar] [CrossRef]
  35. Khan, Z.M.S.H.; Rahman, R.S.; Shumaila; Islam, S.; Zulfequar, M. Hydrothermal treatment of red lentils for the synthesis of fluorescent carbon quantum dots and its application for sensing Fe3+. Opt. Mater. 2019, 91, 386–395. [Google Scholar] [CrossRef]
  36. Kundu, A.; Lee, J.; Park, B.; Ray, C.; Sankar, K.V.; Kim, W.S.; Lee, S.H.; Cho, I.J.; Jun, S.C. Facile approach to synthesize highly fluorescent multicolor emissive carbon dots via surface functionalization for cellular imaging. J. Colloid Interface Sci. 2018, 513, 505–514. [Google Scholar] [CrossRef]
  37. Li, J.; Zuo, G.; Qi, X.; Wei, W.; Pan, X.; Su, T.; Zhang, J.; Dong, W. Selective determination of Ag+ using Salecan derived nitrogen doped carbon dots as a fluorescent probe. Mater. Sci. Eng. C 2017, 77, 508–512. [Google Scholar] [CrossRef]
  38. Shi, L.; Li, L.; Li, X.; Zhang, G.; Zhang, Y.; Dong, C.; Shuang, S. Excitation–independent yellow–fluorescent nitrogen–doped carbon nanodots for biological imaging and paper–based sensing. Sens. Actuators B Chem. 2017, 251, 234–241. [Google Scholar] [CrossRef]
  39. Sun, D.; Ban, R.; Zhang, P.-H.; Wu, G.-H.; Zhang, J.-R.; Zhu, J.-J. Hair fiber as a precursor for synthesizing of sulfur– and nitrogen–co–doped carbon dots with tunable luminescence properties. Carbon 2013, 64, 424–434. [Google Scholar] [CrossRef]
  40. Yin, H.; Gao, D.; Qiu, Y.; Yi, G.; Li, J.; Dong, Y.; Zhang, K.; Xia, Z.; Fu, Q. Carbon source self–heating: Ultrafast, energy–efficient and room temperature synthesis of highly fluorescent N, S–codoped carbon dots for quantitative detection of Fe(iii) ions in biological samples. Nanoscale Adv. 2020, 2, 1483–1492. [Google Scholar] [CrossRef]
  41. Zhao, S.; Wu, S.; Jia, Q.; Huang, L.; Lan, M.; Wang, P.; Zhang, W. Lysosome–targetable carbon dots for highly efficient photothermal/photodynamic synergistic cancer therapy and photoacoustic/two–photon excited fluorescence imaging. Chem. Eng. J. 2020, 388, 124212. [Google Scholar] [CrossRef]
  42. Pandey, S.C.; Kumar, A.; Sahu, S.K. Single Step Green Synthesis of Carbon Dots from Murraya koenigii leaves; A Unique Turn–off Fluorescent contrivance for Selective Sensing of Cd (II) ion. J. Photochem. Photobiol. A Chem. 2020, 400, 112620. [Google Scholar] [CrossRef]
  43. White, B.; Banerjee, S.; O’Brien, S.; Turro, N.J.; Herman, I.P. Zeta–Potential Measurements of Surfactant–Wrapped Individual Single–Walled Carbon Nanotubes. J. Phys. Chem. C 2007, 111, 13684–13690. [Google Scholar] [CrossRef]
  44. Yu, L.; Qu, Y.; Chai, F.; Chen, L. Facile preparation of highly sensitive and selective fluorescent paper sensor for the visual and cyclic detection of Cu2+ and Hg2+. New J. Chem. 2018, 42, 17478–17485. [Google Scholar] [CrossRef]
  45. Dong, G.; Lang, K.; Ouyang, H.; Zhang, W.; Bai, L.; Chen, S.; Zhang, Z.; Gao, Y.; Mu, Z.; Zhao, X. Facile synthesis of N, P–doped carbon dots from maize starch via a solvothermal approach for the highly sensitive detection of Fe3+. RSC Adv. 2020, 10, 33483–33489. [Google Scholar] [CrossRef]
  46. Atchudan, R.; Edison, T.; Aseer, K.R.; Perumal, S.; Karthik, N.; Lee, Y.R. Highly fluorescent nitrogen–doped carbon dots derived from Phyllanthus acidus utilized as a fluorescent probe for label–free selective detection of Fe(3+) ions, live cell imaging and fluorescent ink. Biosens. Bioelectron. 2018, 99, 303–311. [Google Scholar] [CrossRef]
  47. Pang, S.; Liu, S. Dual–emission carbon dots for ratiometric detection of Fe(3+) ions and acid phosphatase. Anal. Chim. Acta 2020, 1105, 155–161. [Google Scholar] [CrossRef]
  48. Qi, H.; Teng, M.; Liu, M.; Liu, S.; Li, J.; Yu, H.; Teng, C.; Huang, Z.; Liu, H.; Shao, Q.; et al. Biomass–derived nitrogen–doped carbon quantum dots: Highly selective fluorescent probe for detecting Fe3+ ions and tetracyclines. J. Colloid Interface Sci. 2019, 539, 332–341. [Google Scholar] [CrossRef]
  49. Jia, J.; Lin, B.; Gao, Y.; Jiao, Y.; Li, L.; Dong, C.; Shuang, S. Highly luminescent N–doped carbon dots from black soya beans for free radical scavenging, Fe3+ sensing and cellular imaging. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2019, 211, 363–372. [Google Scholar] [CrossRef]
  50. Zhao, L.; Wang, Y.; Zhao, X.; Deng, Y.; Xia, Y. Facile Synthesis of Nitrogen–Doped Carbon Quantum Dots with Chitosan for Fluorescent Detection of Fe3+. Polymers 2019, 11, 1731. [Google Scholar] [CrossRef]
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Scheme 1. Schematic illustration of the preparation of N–CDs and applications for Fe3+ detection.
Scheme 1. Schematic illustration of the preparation of N–CDs and applications for Fe3+ detection.
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Figure 1. (A) TEM image of N–CDs, the scale bar is 20 nm. (B) The corresponding size–distribution histogram.
Figure 1. (A) TEM image of N–CDs, the scale bar is 20 nm. (B) The corresponding size–distribution histogram.
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Figure 2. (A) XPS survey spectrum of NCDs. High–resolution spectra C1s (B) and N1s (C) spectra of N–CDs; (D) FT–IR spectra of N–CDs.
Figure 2. (A) XPS survey spectrum of NCDs. High–resolution spectra C1s (B) and N1s (C) spectra of N–CDs; (D) FT–IR spectra of N–CDs.
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Nanomaterials 12 00986 g003 550
Figure 3. (A) UV–vis absorption spectra (black), excitation (blue) and emission (red) spectra of N–CDs. Inset: photographs of aqueous solutions under visible light (left) and 365 nm light UV (right); (B) Fluorescent emission spectra of N–CD aqueous solution under various excitation wavelengths ranging from 340 nm to 500 nm; (C) Fluorescent spectra of N–CDs in different concentrations of sodium chloride; (D) Fluorescent spectra of N–CDs at different pH values.
Figure 3. (A) UV–vis absorption spectra (black), excitation (blue) and emission (red) spectra of N–CDs. Inset: photographs of aqueous solutions under visible light (left) and 365 nm light UV (right); (B) Fluorescent emission spectra of N–CD aqueous solution under various excitation wavelengths ranging from 340 nm to 500 nm; (C) Fluorescent spectra of N–CDs in different concentrations of sodium chloride; (D) Fluorescent spectra of N–CDs at different pH values.
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Figure 4. (A) Fluorescence spectra of N–CDs in the absence and presence of 1 mM Fe3+ at the excitation of 360 nm; insets show the photographs of the N–CD solutions in the absence and presence of Fe3+ under UV light (365 nm); (B) Change in UV–vis absorption spectra of N–CDs upon addition of Fe3+; (C) Speculation on the fluorescence quenching mechanism of N–CDs by Fe3+.
Figure 4. (A) Fluorescence spectra of N–CDs in the absence and presence of 1 mM Fe3+ at the excitation of 360 nm; insets show the photographs of the N–CD solutions in the absence and presence of Fe3+ under UV light (365 nm); (B) Change in UV–vis absorption spectra of N–CDs upon addition of Fe3+; (C) Speculation on the fluorescence quenching mechanism of N–CDs by Fe3+.
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Figure 5. (A) Fluorescent emission spectra of N–CDs solutions with various concentrations of Fe3+ (0–1000 μM); (B) The fluorescence intensity change of N–CDs upon addition of various concentrations of Fe3+ (from top to bottom: 0, 0.1, 1, 10, 25, 50, 100, 150, 200, 250, 400, 500, 750, 1000 μM); (C) The linear calibration plot for fluorescence intensity versus different concentrations of Fe3+ from 0.1 to 400 μM; (D) The difference of fluorescence response (F/F0) of N–CD solution in the presence of various representative metal ions.
Figure 5. (A) Fluorescent emission spectra of N–CDs solutions with various concentrations of Fe3+ (0–1000 μM); (B) The fluorescence intensity change of N–CDs upon addition of various concentrations of Fe3+ (from top to bottom: 0, 0.1, 1, 10, 25, 50, 100, 150, 200, 250, 400, 500, 750, 1000 μM); (C) The linear calibration plot for fluorescence intensity versus different concentrations of Fe3+ from 0.1 to 400 μM; (D) The difference of fluorescence response (F/F0) of N–CD solution in the presence of various representative metal ions.
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Figure 6. Cell viability of A549 cells after incubation with N–CDs with different concentrations. (Untreated cells served as the control, whose viability was set as 100%).
Figure 6. Cell viability of A549 cells after incubation with N–CDs with different concentrations. (Untreated cells served as the control, whose viability was set as 100%).
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Figure 7. Fluorescence microscope images of A549 cells incubated with N–CDs (100 μg·mL−1) in the absence and presence of Fe3+ under bright field (A,D), excitation wavelengths of 405 nm (B,E), 488 nm (C,F) for each horizontal channel, respectively (scale bar: 100 μm).
Figure 7. Fluorescence microscope images of A549 cells incubated with N–CDs (100 μg·mL−1) in the absence and presence of Fe3+ under bright field (A,D), excitation wavelengths of 405 nm (B,E), 488 nm (C,F) for each horizontal channel, respectively (scale bar: 100 μm).
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Table
Table 1. Comparison of sensing performance of different CD–based fluorescent probes for Fe3+ detection.
Table 1. Comparison of sensing performance of different CD–based fluorescent probes for Fe3+ detection.
Fluorescence ProbesSynthetic MethodLinear RangeDetection LimitRef.
SiN–CDsHydrothermal4–500 μM1.68 μM[21]
Lycii Fructus CDsHydrothermal0–30 μM0.021 μM[24]
Phyllanthus acidus N–CDsHydrothermal2–25 μM0.9 μM.[46]
Gallic acid and o–phenylenediamine CDsHydrothermal0 to 50 μM0.8 μM.[47]
Rice residue and glycine N–CQDsHydrothermal3.32 to 32.26 μM0.7462 μM[48]
Black soya beans N–CDs.Pyrolysis0.2–300 μM0.09687 μM[49]
Chitosan N–CQDsHydrothermal0–500 μM0.15 μM[50]
Tea N–CDsHydrothermal0.1–400 μM0.079 μMThis work
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Ge, G.; Li, L.; Chen, M.; Wu, X.; Yang, Y.; Wang, D.; Zuo, S.; Zeng, Z.; Xiong, W.; Guo, C. Green Synthesis of Nitrogen–Doped Carbon Dots from Fresh Tea Leaves for Selective Fe3+ Ions Detection and Cellular Imaging. Nanomaterials 2022, 12, 986. https://doi.org/10.3390/nano12060986

AMA Style

Ge G, Li L, Chen M, Wu X, Yang Y, Wang D, Zuo S, Zeng Z, Xiong W, Guo C. Green Synthesis of Nitrogen–Doped Carbon Dots from Fresh Tea Leaves for Selective Fe3+ Ions Detection and Cellular Imaging. Nanomaterials. 2022; 12(6):986. https://doi.org/10.3390/nano12060986

Chicago/Turabian Style

Ge, Guili, Lin Li, Mingjian Chen, Xu Wu, Yuxin Yang, Dan Wang, Sicheng Zuo, Zhaoyang Zeng, Wei Xiong, and Can Guo. 2022. "Green Synthesis of Nitrogen–Doped Carbon Dots from Fresh Tea Leaves for Selective Fe3+ Ions Detection and Cellular Imaging" Nanomaterials 12, no. 6: 986. https://doi.org/10.3390/nano12060986

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