Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains
2.2. Preparation of AuNPs and CIP-AuNPs
2.3. Characterization of AuNPs and CIP-AuNPs
2.4. Drug Loading Capacity and Encapsulation Efficiency
2.5. Drug Release Efficiency
2.6. Kinetic Analysis of the Drug Release
2.7. In Vitro Stability of CIP-AuNPs
2.8. In Vitro Antibacterial Potential of CIP-AuNPs
2.9. Hemolytic Activity of CIP-AuNPs
2.10. Colonization of E. faecalis in BALB/c Mice
2.11. In Vivo Antibacterial Activity of CIP-AuNPs
2.12. Statistical Analysis
3. Results
3.1. Synthesis of AuNPS and CIP-AuNPs
3.2. CIP Encapsulation Efficiency and CIP Loading Capacity
3.3. Particle Size and Zeta Potential of AuNPs and CIP-AuNPs
3.4. Surface Morphology and Elemental Chemical Composition of AuNPs by SEM–EDS
3.5. Structural Analysis of CIP-AuNPs by FTIR Spectroscopy
3.6. Kinetics of CIP Drug Release from AuNPs
3.7. Stability Tests on CIP-AuNPs
3.8. In Vitro Antibacterial Activity of CIP-AuNPs at the Optimized Dose
3.9. In Vivo Anticolonizing Potential of CIP-AuNPs in an Animal Model
3.10. Hemolytic Activity of CIP-AuNPs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CIP Concentration AuNPs | Encapsulation Efficiency (%) | Loading Capacity (%) |
---|---|---|
0.5 mM | 24.43 | 8.85 |
1.0 mM | 29.30 | 15.60 |
1.5 mM | 30.65 | 28.85 |
2.0 mM | 48.92 | 33.81 |
2.5 mM | 60.83 | 34.54 |
CIP-AuNPs | Z-Average (d. nm) | PDI | St Dev (d. nm) | Zeta Potential (mV) |
---|---|---|---|---|
0.5 mM | 24.43 | 0.26 | 6.21 | −32.1 |
1.0 mM | 24.09 | 0.301 | 6.044 | −33.3 |
1.5 mM | 41 | 0.68 | 10.21 | −19.7 |
2.0 mM | 88.2 | 1.000 | 57.4 | −13.4 |
2.5 mM | 128.2 | 0.48 | 79.18 | −2.12 |
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Nawaz, A.; Ali, S.M.; Rana, N.F.; Tanweer, T.; Batool, A.; Webster, T.J.; Menaa, F.; Riaz, S.; Rehman, Z.; Batool, F.; et al. Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment. Nanomaterials 2021, 11, 3152. https://doi.org/10.3390/nano11113152
Nawaz A, Ali SM, Rana NF, Tanweer T, Batool A, Webster TJ, Menaa F, Riaz S, Rehman Z, Batool F, et al. Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment. Nanomaterials. 2021; 11(11):3152. https://doi.org/10.3390/nano11113152
Chicago/Turabian StyleNawaz, Afrah, Syed Mohsin Ali, Nosheen Fatima Rana, Tahreem Tanweer, Amna Batool, Thomas J. Webster, Farid Menaa, Sundus Riaz, Zahra Rehman, Farhat Batool, and et al. 2021. "Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment" Nanomaterials 11, no. 11: 3152. https://doi.org/10.3390/nano11113152