Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon
Abstract
:1. Introduction
2. Materials and Methods
2.1. OMC Carboxyl Functionalization
2.2. Chemical Amination of Enzymes
2.3. Enzyme Immobilization on OMC-COOH
2.4. Inactivation of GOx/OMC-COOH and GOx-NH2/OMC-COOH
2.5. Fabrication of GOx/OMC-COOH and GOx-NH2/OMC-COOH Bioelectrodes
3. Results
3.1. The Effect of Amination on Enzyme Immobilization
3.2. Characterization of Aminase
3.2.1. 1H NMR Spectral Analysis
3.2.2. Element Composition Analysis
3.2.3. Fourier-Transform Infrared (FTIR) Spectrum Analysis
3.3. The Effect of Amination on Enzyme Immobilization
3.3.1. Temperature
3.3.2. Buffer pH
3.3.3. Time
3.4. Direct Electrochemistry of Nafion/GOx/OMC-COOH and Nafion/GOx-NH2/OMC-COOH Bioelectrodes
4. Discussion
4.1. Effects of GOx Amination
4.2. Improved Characteristics of the Bioelectrode
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Li, J.; Wang, Y.-B.; Qiu, J.-D.; Sun, D.-C.; Xia, X.-H. Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor. Anal. Bioanal. Chem. 2005, 383, 918–922. [Google Scholar] [CrossRef] [PubMed]
- Liu, Q.; Lu, X.; Li, J.; Yao, X.; Li, J. Direct electrochemistry of glucose oxidase and electrochemical biosensing of glucose on quantum dots/carbon nanotubes electrodes. Biosens. Bioelectron. 2007, 22, 3203–3209. [Google Scholar] [CrossRef] [PubMed]
- Tasis, D.; Tagmatarchis, N.; Bianco, A.; Prato, M. Chemistry of Carbon Nanotubes. Chem. Rev. 2006, 106, 1105–1136. [Google Scholar] [CrossRef] [PubMed]
- Zhang, D.; Chen, X.; Ma, W.; Yang, T.; Li, D.; Dai, B.; Zhang, Y. Direct electrochemistry of glucose oxidase based on one step electrodeposition of reduced graphene oxide incorporating polymerized l-lysine and its application in glucose sensing. Mater. Sci. Eng. C 2019, 104, 109880. [Google Scholar] [CrossRef] [PubMed]
- Zhao, H.-Z.; Sun, J.-J.; Song, J.; Yang, Q.-Z. Direct electron transfer and conformational change of glucose oxidase on carbon nanotube-based electrodes. Carbon 2010, 48, 1508–1514. [Google Scholar] [CrossRef]
- Beissenhirtz, M.K.; Scheller, F.W.; Stöcklein, W.F.M.; Kurth, D.G.; Möhwald, H.; Lisdat, F. Electroactive Cytochrome c Multilayers within a Polyelectrolyte Assembly. Angew. Chem. Int. Ed. 2004, 43, 4357–4360. [Google Scholar] [CrossRef]
- Marcus, R.A.; Sutin, N. Electron transfers in chemistry and biology. Biochim. Biophys. Acta (BBA)—Rev. Bioenerg. 1985, 811, 265–322. [Google Scholar] [CrossRef]
- Liu, Y.; Wang, M.; Zhao, F.; Xu, Z.; Dong, S. The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix. Biosens. Bioelectron. 2005, 21, 984–988. [Google Scholar] [CrossRef]
- Deng, C.; Chen, J.; Chen, X.; Xiao, C.; Nie, L.; Yao, S. Direct electrochemistry of glucose oxidase and biosensing for glucose based on boron-doped carbon nanotubes modified electrode. Biosens. Bioelectron. 2008, 23, 1272–1277. [Google Scholar] [CrossRef]
- Kang, X.; Mai, Z.; Zou, X.; Cai, P.; Mo, J. A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold–platinum alloy nanoparticles/multiwall carbon nanotubes. Anal. Biochem. 2007, 369, 71–79. [Google Scholar] [CrossRef]
- Ren, Q.; Feng, L.; Fan, R.; Ge, X.; Sun, Y. Water-dispersible triethylenetetramine-functionalized graphene: Preparation, characterization and application as an amperometric glucose sensor. Mater. Sci. Eng. C 2016, 68, 308–316. [Google Scholar] [CrossRef]
- Hitaishi, V.P.; Clement, R.; Bourassin, N.; Baaden, M.; De Poulpiquet, A.; Sacquin-Mora, S.; Ciaccafava, A.; Lojou, E. Controlling Redox Enzyme Orientation at Planar Electrodes. Catalysts 2018, 8, 192. [Google Scholar] [CrossRef] [Green Version]
- Abian, O.; Grazú, V.; Hermoso, J.; González, R.; García, J.L.; Fernández-Lafuente, R.; Guisán, J.M. Stabilization of penicillin G acylase from Escherichia coli: Site-directed mutagenesis of the protein surface to increase multipoint covalent attachment. Appl. Environ. Microbiol. 2004, 70, 1249–1251. [Google Scholar] [CrossRef] [Green Version]
- López-Gallego, F.; Montes, T.; Fuentes, M.; Alonso, N.; Grazu, V.; Betancor, L.; Guisán, J.M.; Fernández-Lafuente, R. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. J. Biotechnol. 2005, 116, 1–10. [Google Scholar] [CrossRef]
- Mazurenko, I.; Hitaishi, V.P.; Lojou, E. Recent advances in surface chemistry of electrodes to promote direct enzymatic bioelectrocatalysis. Curr. Opin. Electrochem. 2020, 19, 113–121. [Google Scholar] [CrossRef]
- Meder, F.; Daberkow, T.; Treccani, L.; Wilhelm, M.; Schowalter, M.; Rosenauer, A.; Mädler, L.; Rezwan, K. Protein adsorption on colloidal alumina particles functionalized with amino, carboxyl, sulfonate and phosphate groups. Acta Biomater. 2012, 8, 1221–1229. [Google Scholar] [CrossRef] [PubMed]
- Meder, F.; Hintz, H.; Koehler, Y.; Schmidt, M.M.; Treccani, L.; Dringen, R.; Rezwan, K. Adsorption and Orientation of the Physiological Extracellular Peptide Glutathione Disulfide on Surface Functionalized Colloidal Alumina Particles. J. Am. Chem. Soc. 2013, 135, 6307–6316. [Google Scholar] [CrossRef] [PubMed]
- Gessner, A.; Waicz, R.; Lieske, A.; Paulke, B.R.; Mäder, K.; Müller, R.H. Nanoparticles with decreasing surface hydrophobicities: Influence on plasma protein adsorption. Int. J. Pharm. 2000, 196, 245–249. [Google Scholar] [CrossRef]
- Olloqui-Sariego, J.L.; Calvente, J.J.; Andreu, R. Immobilizing Redox Enzymes at Mesoporous and Nanoestructured Electrodes. Curr. Opin. Electrochem. 2020, 26, 100658. [Google Scholar] [CrossRef]
- Fernandez-Lorente, G.; Godoy, C.A.; Mendes, A.A.; Lopez-Gallego, F.; Grazu, V.; de las Rivas, B.; Palomo, J.M.; Hermoso, J.; Fernandez-Lafuente, R.; Guisan, J.M. Solid-Phase Chemical Amination of a Lipase from Bacillus thermocatenulatus To Improve Its Stabilization via Covalent Immobilization on Highly Activated Glyoxyl-Agarose. Biomacromolecules 2008, 9, 2553–2561. [Google Scholar] [CrossRef]
- Ashjari, M.; Mohammadi, M.; Badri, R. Chemical amination of Rhizopus oryzae lipase for multipoint covalent immobilization on epoxy-functionalized supports: Modulation of stability and selectivity. J. Mol. Catal. B Enzym. 2015, 115, 128–134. [Google Scholar] [CrossRef]
- Rodrigues, R.C.; Godoy, C.A.; Volpato, G.; Ayub, M.A.Z.; Fernandez-Lafuente, R.; Guisan, J.M. Immobilization–stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination. Process Biochem. 2009, 44, 963–968. [Google Scholar] [CrossRef]
- Othman, A.M.; Wollenberger, U. Amperometric biosensor based on coupling aminated laccase to functionalized carbon nanotubes for phenolics detection. Int. J. Biol. Macromol. 2020, 153, 855–864. [Google Scholar] [CrossRef] [PubMed]
- Kjeang, E.; Michel, R.; Harrington, D.A.; Djilali, N.; Sinton, D. A Microfluidic Fuel Cell with Flow-Through Porous Electrodes. J. Am. Chem. Soc. 2008, 130, 4000–4006. [Google Scholar] [CrossRef]
- Yang, X.; Ma, X.; Wang, K.; Wu, D.; Lei, Z.; Feng, C. Eighteen-month assessment of 3D graphene oxide aerogel-modified 3D graphite fiber brush electrode as a high-performance microbial fuel cell anode. Electrochim. Acta 2016, 210, 846–853. [Google Scholar] [CrossRef]
- Aldalbahi, A.; Rahaman, M.; Almoiqli, M.; Hamedelniel, A.; Alrehaili, A. Single-Walled Carbon Nanotube (SWCNT) Loaded Porous Reticulated Vitreous Carbon (RVC) Electrodes Used in a Capacitive Deionization (CDI) Cell for Effective Desalination. Nanomaterials 2018, 8, 527. [Google Scholar] [CrossRef] [Green Version]
- Mishra, A.; Shetti, N.P.; Basu, S.; Reddy, K.R.; Aminabhavi, T.M. Carbon Cloth-based Hybrid Materials as Flexible Electrochemical Supercapacitors. ChemElectroChem 2019, 6, 5771–5786. [Google Scholar] [CrossRef]
- Mazurenko, I.; Monsalve, K.; Infossi, P.; Giudici-Orticoni, M.-T.; Topin, F.; Mano, N.; Lojou, E. Impact of substrate diffusion and enzyme distribution in 3D-porous electrodes: A combined electrochemical and modelling study of a thermostable H2/O2 enzymatic fuel cell. Energy Environ. Sci. 2017, 10, 1966–1982. [Google Scholar] [CrossRef]
- Mendes, T.P.P.; Lobón, G.S.; Lima, L.A.S.; Guerra, N.K.M.; Carvalho, G.A.; Freitas, E.M.M.; Pinto, M.C.X.; Pereira, I.; Vaz, B.G. Mass spectrometry-based biosensing using pencil graphite rods. Microchem. J. 2021, 164, 106077. [Google Scholar] [CrossRef]
- Arlyapov, V.A.; Khar’kova, A.S.; Abramova, T.N.; Kuznetsova, L.S.; Ilyukhina, A.S.; Zaitsev, M.G.; Machulin, A.V.; Reshetilov, A.N. A Hybrid Redox-Active Polymer Based on Bovine Serum Albumin, Ferrocene, Carboxylated Carbon Nanotubes, and Glucose Oxidase. J. Anal. Chem. 2020, 75, 1189–1200. [Google Scholar] [CrossRef]
- Wang, J. Nanomaterial-based electrochemical biosensors. Analyst 2005, 130, 421–426. [Google Scholar] [CrossRef] [PubMed]
- Kumar, R.; Bhuvana, T.; Mishra, G.; Sharma, A. A polyaniline wrapped aminated graphene composite on nickel foam as three-dimensional electrodes for enzymatic microfuel cells. RSC Adv. 2016, 6, 73496–73505. [Google Scholar] [CrossRef]
- You, H.; Mu, Z.; Zhao, M.; Zhou, J.; Chen, Y.; Bai, L. Voltammetric aptasensor for sulfadimethoxine using a nanohybrid composed of multifunctional fullerene, reduced graphene oxide and Pt@Au nanoparticles, and based on direct electron transfer to the active site of glucose oxidase. Microchim. Acta 2018, 186, 1. [Google Scholar] [CrossRef]
- Davis, J.J.; Green, M.L.H.; Allen, O.; Hill, H.; Leung, Y.C.; Sadler, P.J.; Sloan, J.; Xavier, A.V.; Tsang, S.C. The immobilisation of proteins in carbon nanotubes. Inorg. Chim. Acta 1998, 272, 261–266. [Google Scholar] [CrossRef]
- Zhang, K.; Zhou, H.; Hu, P.; Lu, Q. The direct electrochemistry and bioelectrocatalysis of nitrate reductase at a gold nanoparticles/aminated graphene sheets modified glassy carbon electrode. RSC Adv. 2019, 9, 37207–37213. [Google Scholar] [CrossRef] [Green Version]
- Meder, F.; Kaur, S.; Treccani, L.; Rezwan, K. Controlling Mixed-Protein Adsorption Layers on Colloidal Alumina Particles by Tailoring Carboxyl and Hydroxyl Surface Group Densities. Langmuir 2013, 29, 12502–12510. [Google Scholar] [CrossRef]
- Lopes, I.; Piao, L.; Stievano, L.; Lambert, J.-F. Adsorption of Amino Acids on Oxide Supports: A Solid-State NMR Study of Glycine Adsorption on Silica and Alumina. J. Phys. Chem. C 2009, 113, 18163–18172. [Google Scholar] [CrossRef]
- Thyparambil, A.A.; Wei, Y.; Latour, R.A. Determination of Peptide–Surface Adsorption Free Energy for Material Surfaces Not Conducive to SPR or QCM using AFM. Langmuir 2012, 28, 5687–5694. [Google Scholar] [CrossRef] [Green Version]
- Soliman, W.; Bhattacharjee, S.; Kaur, K. Adsorption of an Antimicrobial Peptide on Self-Assembled Monolayers by Molecular Dynamics Simulation. J. Phys. Chem. B 2010, 114, 11292–11302. [Google Scholar] [CrossRef]
- Lv, C.; Li, S.; Liu, L.; Zhu, X.; Yang, X. Enhanced Electrochemical Characteristics of the Glucose Oxidase Bioelectrode Constructed by Carboxyl-Functionalized Mesoporous Carbon. Sensors 2020, 20, 3365. [Google Scholar] [CrossRef]
- Vinu, A.; Hossian, K.Z.; Srinivasu, P.; Miyahara, M.; Anandan, S.; Gokulakrishnan, N.; Mori, T.; Ariga, K.; Balasubramanian, V.V. Carboxy-mesoporous carbon and its excellent adsorption capability for proteins. J. Mater. Chem. 2007, 17, 1819–1825. [Google Scholar] [CrossRef]
- Deng, S.; Jian, G.; Lei, J.; Hu, Z.; Ju, H. A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-doped carbon nanotubes. Biosens. Bioelectron. 2009, 25, 373–377. [Google Scholar] [CrossRef]
- Afshar, H.A.; Ghaee, A. Preparation of aminated chitosan/alginate scaffold containing halloysite nanotubes with improved cell attachment. Carbohydr. Polym. 2016, 151, 1120–1131. [Google Scholar] [CrossRef] [PubMed]
- de Morais Júnior, W.G.; Terrasan, C.R.F.; Fernández-Lorente, G.; Guisán, J.M.; Ribeiro, E.J.; de Resende, M.M.; Pessela, B.C. Solid-phase amination of Geotrichum candidum lipase: Ionic immobilization, stabilization and fish oil hydrolysis for the production of Omega-3 polyunsaturated fatty acids. Eur. Food Res. Technol. 2017, 243, 1375–1384. [Google Scholar] [CrossRef]
- Rodrigues, R.C.; Barbosa, O.; Ortiz, C.; Berenguer-Murcia, Á.; Torres, R.; Fernandez-Lafuente, R. Amination of enzymes to improve biocatalyst performance: Coupling genetic modification and physicochemical tools. RSC Adv. 2014, 4, 38350–38374. [Google Scholar] [CrossRef] [Green Version]
- Hernandez, K.; Fernandez-Lafuente, R. Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance. Enzym. Microb. Technol. 2011, 48, 107–122. [Google Scholar] [CrossRef] [PubMed]
- Rosenbohm, C.; Lundt, I.; Christensen, T.I.E.; Young, N.G. Chemically methylated and reduced pectins: Preparation, characterisation by 1H NMR spectroscopy, enzymatic degradation, and gelling properties. Carbohydr. Res. 2003, 338, 637–649. [Google Scholar] [CrossRef]
- Hoare, D.G.; Olson, A.; Koshland, D.E. The reaction of hydroxamic acids with water-soluble carbodiimides. A lossen rearrangement. J. Am. Chem. Soc. 1968, 90, 1638–1643. [Google Scholar] [CrossRef]
- Spalding, K.; Bonnier, F.; Bruno, C.; Blasco, H.; Board, R.; Benz-de Bretagne, I.; Byrne, H.J.; Butler, H.J.; Chourpa, I.; Radhakrishnan, P.; et al. Enabling quantification of protein concentration in human serum biopsies using attenuated total reflectance—Fourier transform infrared (ATR-FTIR) spectroscopy. Vib. Spectrosc. 2018, 99, 50–58. [Google Scholar] [CrossRef] [Green Version]
- Movasaghi, Z.; Rehman, S.; ur Rehman, D.I. Fourier transform infrared (FTIR) spectroscopy of biological tissues. Appl. Spectrosc. Rev. 2008, 43, 134–179. [Google Scholar] [CrossRef]
- Schmidt, M.P.; Martínez, C.E. Kinetic and Conformational Insights of Protein Adsorption onto Montmorillonite Revealed Using in Situ ATR-FTIR/2D-COS. Langmuir 2016, 32, 7719–7729. [Google Scholar] [CrossRef] [PubMed]
- Andrade, J.; Pereira, C.G.; de Almeida Junior, J.C.; Viana, C.C.R.; de Oliveira Neves, L.N.; da Silva, P.H.F.; Bell, M.J.V.; de Carvalho dos Anjos, V. FTIR-ATR determination of protein content to evaluate whey protein concentrate adulteration. LWT 2019, 99, 166–172. [Google Scholar] [CrossRef]
- Barth, A.; Zscherp, C. What vibrations tell about proteins. Q. Rev. Biophys. 2002, 35, 369–430. [Google Scholar] [CrossRef] [PubMed]
- Grdadolnik, J.; Maréchal, Y. Bovine serum albumin observed by infrared spectrometry. I. Methodology, structural investigation, and water uptake. Biopolymers 2001, 62, 40–53. [Google Scholar] [CrossRef]
- Shrestha, B.K.; Ahmad, R.; Mousa, H.M.; Kim, I.-G.; Kim, J.I.; Neupane, M.P.; Park, C.H.; Kim, C.S. High-performance glucose biosensor based on chitosan-glucose oxidase immobilized polypyrrole/Nafion/functionalized multi-walled carbon nanotubes bio-nanohybrid film. J. Colloid Interface Sci. 2016, 482, 39–47. [Google Scholar] [CrossRef] [PubMed]
- Tian, K.; Liu, H.; Dong, Y.; Chu, X.; Wang, S. Amperometric detection of glucose based on immobilizing glucose oxidase on g-C3N4 nanosheets. Colloids Surf. A Physicochem. Eng. Asp. 2019, 581, 123808. [Google Scholar] [CrossRef]
- Sağlam, Ö.; Kızılkaya, B.; Uysal, H.; Dilgin, Y. Biosensing of glucose in flow injection analysis system based on glucose oxidase-quantum dot modified pencil graphite electrode. Talanta 2016, 147, 315–321. [Google Scholar] [CrossRef]
- Wang, K.; Yang, H.; Zhu, L.; Ma, Z.; Xing, S.; Lv, Q.; Liao, J.; Liu, C.; Xing, W. Direct electron transfer and electrocatalysis of glucose oxidase immobilized on glassy carbon electrode modified with Nafion and mesoporous carbon FDU-15. Electrochim. Acta 2009, 54, 4626–4630. [Google Scholar] [CrossRef]
- Mazar, F.M.; Alijanianzadeh, M.; Molaeirad, A.; Heydari, P. Development of Novel Glucose oxidase Immobilization on Graphene/Gold nanoparticles/Poly Neutral red modified electrode. Process Biochem. 2017, 56, 71–80. [Google Scholar] [CrossRef]
- Terse-Thakoor, T.; Komori, K.; Ramnani, P.; Lee, I.; Mulchandani, A. Electrochemically Functionalized Seamless Three-Dimensional Graphene-Carbon Nanotube Hybrid for Direct Electron Transfer of Glucose Oxidase and Bioelectrocatalysis. Langmuir 2015, 31, 13054–13061. [Google Scholar] [CrossRef]
- Jędrzak, A.; Rębiś, T.; Klapiszewski, Ł.; Zdarta, J.; Milczarek, G.; Jesionowski, T. Carbon paste electrode based on functional GOx/silica-lignin system to prepare an amperometric glucose biosensor. Sens. Actuators B Chem. 2018, 256, 176–185. [Google Scholar] [CrossRef]
- Amatatongchai, M.; Sroysee, W.; Chairam, S.; Nacapricha, D. Amperometric flow injection analysis of glucose using immobilized glucose oxidase on nano-composite carbon nanotubes-platinum nanoparticles carbon paste electrode. Talanta 2017, 166, 420–427. [Google Scholar] [CrossRef]
- Saravanan, N.; Mayuri, P.; Kumar, A.S. Improved Electrical Wiring of Glucose Oxidase Enzyme with an in-Situ Immobilized Mn(1,10-Phenanthroline)2Cl2-Complex/Multiwalled Carbon Nanotube-Modified Electrode Displaying Superior Performance to Os-Complex for High-Current Sensitivity Bioelectrocatalytic and Biofuel Cell Applications. ACS Appl. Bio Mater. 2018, 1, 1758–1767. [Google Scholar]
- Laviron, E. General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems. J. Electroanal. Chem. Interfacial Electrochem. 1979, 101, 19–28. [Google Scholar] [CrossRef]
- Hess, A.; Roode-Gutzmer, Q.; Heubner, C.; Schneider, M.; Michaelis, A.; Bobeth, M.; Cuniberti, G. Determination of state of charge-dependent asymmetric Butler–Volmer kinetics for LixCoO2 electrode using GITT measurements. J. Power Sources 2015, 299, 156–161. [Google Scholar] [CrossRef]
- Mateo, C.; Abian, O.; Bernedo, M.; Cuenca, E.; Fuentes, M.; Fernandez-Lorente, G.; Palomo, J.M.; Grazu, V.; Pessela, B.C.C.; Giacomini, C.; et al. Some special features of glyoxyl supports to immobilize proteins. Enzym. Microb. Technol. 2005, 37, 456–462. [Google Scholar] [CrossRef]
- Fernandez-Lafuente, R.; Rosell, C.M.; Rodriguez, V.; Santana, C.; Soler, G.; Bastida, A.; Guisán, J.M. Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method. Enzym. Microb. Technol. 1993, 15, 546–550. [Google Scholar] [CrossRef]
- Lu, F.; Gu, L.; Meziani, M.J.; Wang, X.; Luo, P.G.; Veca, L.M.; Cao, L.; Sun, Y.-P. Advances in Bioapplications of Carbon Nanotubes. Adv. Mater. 2009, 21, 139–152. [Google Scholar] [CrossRef]
- Othman, A.M.; González-Domínguez, E.; Sanromán, Á.; Correa-Duarte, M.; Moldes, D. Immobilization of laccase on functionalized multiwalled carbon nanotube membranes and application for dye decolorization. RSC Adv. 2016, 6, 114690–114697. [Google Scholar] [CrossRef]
- González-Domínguez, E.; Comesaña-Hermo, M.; Mariño-Fernández, R.; Rodríguez-González, B.; Arenal, R.; Salgueiriño, V.; Moldes, D.; Othman, A.M.; Pérez-Lorenzo, M.; Correa-Duarte, M.A. Hierarchical Nanoplatforms for High-Performance Enzyme Biocatalysis under Denaturing Conditions. ChemCatChem 2016, 8, 1264–1268. [Google Scholar] [CrossRef]
- Lopez, R.J.; Babanova, S.; Ulyanova, Y.; Singhal, S.; Atanassov, P. Improved Interfacial Electron Transfer in Modified Bilirubin Oxidase Biocathodes. ChemElectroChem 2014, 1, 241–248. [Google Scholar] [CrossRef]
- González-Gaitán, C.; Ruiz-Rosas, R.; Morallón, E.; Cazorla-Amorós, D. Effects of the surface chemistry and structure of carbon nanotubes on the coating of glucose oxidase and electrochemical biosensors performance. RSC Adv. 2017, 7, 26867–26878. [Google Scholar] [CrossRef] [Green Version]
- Wang, L.; Bai, J.; Bo, X.; Zhang, X.; Guo, L. A novel glucose sensor based on ordered mesoporous carbon–Au nanoparticles nanocomposites. Talanta 2011, 83, 1386–1391. [Google Scholar] [CrossRef] [PubMed]
- Jin-Zhong, X.; Jun-Jie, Z.; Qiang, W.; Zheng, H.; Hong-Yuan, C. Direct Electron Transfer between Glucose Oxidase and Multi-walled Carbon Nanotubes. Chin. J. Chem. 2003, 21, 1088–1091. [Google Scholar] [CrossRef]
- Njoko, N.; Louzada, M.; Britton, J.; Khene, S.; Nyokong, T.; Mashazi, P. Bioelectrocatalysis and surface analysis of gold coated with nickel oxide/hydroxide and glucose oxidase towards detection of glucose. Colloids Surf. B Biointerfaces 2020, 190, 110981. [Google Scholar] [CrossRef]
- Li, J.; Liu, Y.; Tang, X.; Xu, L.; Min, L.; Xue, Y.; Hu, X.; Yang, Z. Multiwalled carbon nanotubes coated with cobalt(II) sulfide nanoparticles for electrochemical sensing of glucose via direct electron transfer to glucose oxidase. Microchim. Acta 2020, 187, 80. [Google Scholar] [CrossRef]
- Haghighi, N.; Hallaj, R.; Salimi, A. Immobilization of glucose oxidase onto a novel platform based on modified TiO2 and graphene oxide, direct electrochemistry, catalytic and photocatalytic activity. Mater. Sci. Eng. C 2017, 73, 417–424. [Google Scholar] [CrossRef] [PubMed]
- Lin, J.; He, C.; Zhao, Y.; Zhang, S. One-step synthesis of silver nanoparticles/carbon nanotubes/chitosan film and its application in glucose biosensor. Sens. Actuators B Chem. 2009, 137, 768–773. [Google Scholar] [CrossRef]
- Mani, V.; Govindasamy, M.; Chen, S.-M.; Chen, T.-W.; Kumar, A.S.; Huang, S.-T. Core-shell heterostructured multiwalled carbon nanotubes@reduced graphene oxide nanoribbons/chitosan, a robust nanobiocomposite for enzymatic biosensing of hydrogen peroxide and nitrite. Sci. Rep. 2017, 7, 11910. [Google Scholar] [CrossRef]
Type | Quantity of the Immobilized Enzyme (GOx mg/OMC g) | Immobilized Enzyme Activity (U/g) | Unit Free Enzyme Activity (U/mg) |
---|---|---|---|
GOx | / | / | 417.74 ± 22.22 |
GOx-NH2 | / | / | 364.41 ± 4.44 |
GOx/OMC-COOH | 44.72 ± 0.88 | 58,290.47 ± 3055.42 | 1303.52 ± 68.33 |
GOx-NH2/OMC-COOH | 124.01 ± 1.49 | 67,956.17 ± 4687.75 | 547.97 ± 37.80 |
Electrode | Anodic Peak Current | Reduction Peak Current | GOx Surface Coverage (Γ) | ks | References |
---|---|---|---|---|---|
Nafion/GOx-NH2/OMC-COOH | 0.324 mA | −0.394 mA | 2.91 × 10−9 mol·cm−2 | 2.54 s−1 | This study |
GC/TiO2-IOSL/GOD | 0.020 mA | −0.022 mA | 1.23 × 10−9 mol·cm−2 | 1.74 s−1 | [77] |
RGO-AuNPs/PNR/GOx | 0.006 mA | −0.012 mA | 3.06 × 10−9 mol·cm−11 | 1.73 s−1 | [59] |
GOD/HRP/Ag/CNT/ITO | 0.022 mA | −0.008 mA | 3.52 × 10−10 mol·cm−2 | 1.76 s−1 | [78] |
GCE/MnO2 -G/PTA/Frt/GOx | 0.120 mA | −0.180 mA | 9.7 × 10−10 mol·cm−2 | 1.96 s−1 | [79] |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Lv, C.; Yang, X.; Wang, Z.; Ying, M.; Han, Q.; Li, S. Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon. Nanomaterials 2021, 11, 3086. https://doi.org/10.3390/nano11113086
Lv C, Yang X, Wang Z, Ying M, Han Q, Li S. Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon. Nanomaterials. 2021; 11(11):3086. https://doi.org/10.3390/nano11113086
Chicago/Turabian StyleLv, Chuhan, Xuewei Yang, Zongkang Wang, Ming Ying, Qingguo Han, and Shuangfei Li. 2021. "Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon" Nanomaterials 11, no. 11: 3086. https://doi.org/10.3390/nano11113086