Quantitative Phase Imaging Using Digital Holographic Microscopy to Assess the Degree of Intestinal Inflammation in Patients with Ulcerative Colitis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design and Study Population
2.2. Histopathological Preparation
2.3. Setup for Digital Holographic Microscopy, Sample Preparation, and Quantitative Phase Imaging
2.4. Determination of the Refractive Index in Mucosal Biopsies
2.5. Statistical Analysis
3. Results
3.1. Study Population
3.2. Microscopically Analyzed Biopsies
3.3. Correlation of Refractive Index with Histological Scorings and Endoscopic Findings
3.4. Capability of Digital Holographic Microscopy to Distinguish Ulcerative Colitis in Remission from Active Disease
3.5. Correlations of the Refractive Index with Laboratory Inflammation Markers and Clinical Findings
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variables | Patients (N = 21) |
---|---|
Age in years (Mean ± SEM) | 47.3 ± 3.3 |
Female | 6 (29%) |
Male | 15 (71%) |
Patients undergoing a colonoscopy | 13 (62%) |
Patients undergoing a sigmoidoscopy | 8 (38%) |
Duration of disease in months (Mean ± SEM) | 127.4 ± 26.9 |
Indication for examination | |
Colorectal dysplasia screening | 5 (24%) |
Control examination after therapy change | 7 (33%) |
Exacerbation of clinical symptoms | 9 (43%) |
Disease activity | |
Average Mayo endoscopic subscore at most distal biopsy site (Mean ± SEM) | 1.0 ± 0.2 |
Average Mayo score (Mean ± SEM) | 4.1 ± 0.8 |
Average Nancy index at most distal biopsy site (Mean ± SEM) | 1.8 ± 0.4 |
Disease localization according to Montreal Classification | |
Extensity 1; ulcerative proctitis | 3 (14%) |
Extensity 2; left-sided UC | 5 (24%) |
Extensity 3; pancolitis | 13 (62%) |
Medical treatment | |
Biologics | |
Antitumor necrosis factor agents (Adalimumab, Infliximab, and Golimumab) | 9 (43%) |
Integrin α4β7 antagonist (Vedolizumab) | 5 (24%) |
Immunosuppressants | |
Azathioprine | 2 (10%) |
Cyclosporine A | 1 (5%) |
Small molecules | |
Tofacitinib | 1 (5%) |
Corticosteroids | |
Oral | 1 (5%) |
Rectal | 2 (19%) |
Aminosalicylates | |
Mesalazine oral | 15 (71%) |
Mesalazine rectal | 4 (19%) |
Sulfasalazine oral | 1 (5%) |
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Bokemeyer, A.; Buskermolen, J.; Ketelhut, S.; Tepasse, P.-R.; Vollenberg, R.; Trebicka, J.; Schmidt, H.H.; Vieth, M.; Bettenworth, D.; Kemper, B. Quantitative Phase Imaging Using Digital Holographic Microscopy to Assess the Degree of Intestinal Inflammation in Patients with Ulcerative Colitis. J. Clin. Med. 2023, 12, 4067. https://doi.org/10.3390/jcm12124067
Bokemeyer A, Buskermolen J, Ketelhut S, Tepasse P-R, Vollenberg R, Trebicka J, Schmidt HH, Vieth M, Bettenworth D, Kemper B. Quantitative Phase Imaging Using Digital Holographic Microscopy to Assess the Degree of Intestinal Inflammation in Patients with Ulcerative Colitis. Journal of Clinical Medicine. 2023; 12(12):4067. https://doi.org/10.3390/jcm12124067
Chicago/Turabian StyleBokemeyer, Arne, Joost Buskermolen, Steffi Ketelhut, Phil-Robin Tepasse, Richard Vollenberg, Jonel Trebicka, Hartmut H. Schmidt, Michael Vieth, Dominik Bettenworth, and Björn Kemper. 2023. "Quantitative Phase Imaging Using Digital Holographic Microscopy to Assess the Degree of Intestinal Inflammation in Patients with Ulcerative Colitis" Journal of Clinical Medicine 12, no. 12: 4067. https://doi.org/10.3390/jcm12124067