Verification of Blood-Brain Barrier Disruption Based on the Clinical Validation Platform Using a Rat Model with Human Skull
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Subjects and the Human Skull
2.2. Transducer Characteristics
2.3. BBBD System
2.4. Ultrasound Acoustic Characteristics Measurement
2.5. BBBD Experiment
2.6. MRI
2.7. Acoustic Cavitation
2.8. Histology
2.9. Statistical Analysis
2.10. Numerical Simulations of Acoustic Model
3. Results
3.1. Transducer Characteristics
3.2. Ultrasound Acoustic Characteristic Analysis According to Existence of Human Skull
3.3. BBBD
3.4. Passive Acoustic Cavitation
3.5. Histological Analysis
3.6. Acoustic Simulation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Experiments | Pilot Study | Evans Blue for BBBD Confirmation | Cavitation Acquisition | MRI and Histology |
---|---|---|---|---|
Animal number | 4 | 10 | 2 | 4 |
Speed of Sound (m s−1) | Density (kg m−3) | Attenuation (dB MHz−1.43 cm−1) |
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Park, C.Y.; Seo, H.; Lee, E.-H.; Han, M.; Choi, H.; Park, K.-S.; Yoon, S.-Y.; Chang, S.H.; Park, J. Verification of Blood-Brain Barrier Disruption Based on the Clinical Validation Platform Using a Rat Model with Human Skull. Brain Sci. 2021, 11, 1429. https://doi.org/10.3390/brainsci11111429
Park CY, Seo H, Lee E-H, Han M, Choi H, Park K-S, Yoon S-Y, Chang SH, Park J. Verification of Blood-Brain Barrier Disruption Based on the Clinical Validation Platform Using a Rat Model with Human Skull. Brain Sciences. 2021; 11(11):1429. https://doi.org/10.3390/brainsci11111429
Chicago/Turabian StylePark, Chan Yuk, Hyeon Seo, Eun-Hee Lee, Mun Han, Hyojin Choi, Ki-Su Park, Sang-Youl Yoon, Sung Hyun Chang, and Juyoung Park. 2021. "Verification of Blood-Brain Barrier Disruption Based on the Clinical Validation Platform Using a Rat Model with Human Skull" Brain Sciences 11, no. 11: 1429. https://doi.org/10.3390/brainsci11111429