Analysis of Content Profiles, Antioxidant and Anticancer Properties in Endemic Hypericum salsolifolium
Round 1
Reviewer 1 Report
The manuscript can be accepted for publishing in the Journal. However, there are so many typos in the text, and the authors should polish the writing. Please find the attachment.
Comments for author File: 
Comments.pdf
Moderate editing of English language required
Author Response
Dear Reviewer
thank you ...
A1- We have corrected typos in the text and polished the writing. We made editing of English and also made editing according to your attachment.
Reviewer 2 Report
THE PAPER IS GOOD AND PRESENTS NEW STUDY WITH FEW NEW PERSPECTIVE. HOWEVER IT NEEDS EXTENSIVE ENGLISH EDITING FOR SYNTAX, GRAMMER AND BETTER UNDERSTANDING OF THE PAPER.
PROVIDE THE SUPPLEMENTARY FILES FOR HPLC METHOD AND LC MS MS
major revisions with english language
Author Response
Dear Reviewer
Thank you so much.
A-1- We have made editing extensive english language, syntax, grammer and BETTER UNDERSTANDING OF THE PAPER . We provided the supplementary files for HPLC method and LC MS MS, in Material and Method. (2.10 and 2.11; numaration)
Reviewer 3 Report
Dear authors,
After the review process, I have several comments: the paper should be arranged in the journal format - see the reference style; the introduction should be updated, you should include data related to the bioavailability of major functional compounds; from table 4, you should include statistical data; table 2 is not in the corrected format; figure 2 could be moved as supplementary files; comments related to the bioactive potential of functional extracts and bioavailability of phenolic compounds should be included in discussions; the relation with microbiota bioactivity could have a target at the future valorization of the paper; negative points could be included in discussions.
Best regards!
In general, it is ok. Only minor modifications.
Author Response
Dear Reviewer, Thank you so much.
Q1- After the review process, I have several comments: the paper should be arranged in the journal format - see the reference style;
A1- WE ARRANGED IN THE JOURNAL FORMAT
2-the introduction should be updated, you should include data related to the bioavailability of major functional compounds;
A2-WE MADE UPDATED AND RELATED TO THE BIOVAILABITY OF MAJOR FUNCTIONEL COMPOUNDS
3-from table 4, you should include statistical data;
A3-WE MADE IT
4-table 2 is not in the corrected format;
A4- WE CORRECTED IT
5-figure 2 could be moved as supplementary files;
A5-THAT IS CLEARLY
6-comments related to the bioactive potential of functional extracts and bioavailability of phenolic compounds should be included in discussions;
A6-WE EXPLAINED IN DISCUSSIONS
7-the relation with microbiota bioactivity could have a target at the future valorization of the paper;
A7- WE MADE IT
8-negative points could be included in discussions.
A8- WE MADE IT
Reviewer 4 Report
This is an interesting study important from practical point of view. However, the study contains some shortness’s which need to be answered. Numerous editorial issues also requires a lot of attention.
Line 2 - Hypericum salsolifolium should be wrtitten in italics
Line 3 – Please use superspcripts to identify the authors, please also remove orcid numbers from their affiliations
Line 9 – please expand all acronyms in Abstract
Line 31 and the rest of the manuscript – All the reference list and citation style must follow journal guidelines.
Line 56 – please present your hypothesis properly
Line 62 – please provide more details for all reagents used in the experiment (manufacturers’ codes, concentrations, etc.)
Line 83 – Please change to “oC”
Line 85 – please explain what is TPC?
Line 132 – what was a source of cell lines ?
Line 172 – what was post-hoc test used in ANOVA?
Figure 2 – please add scale bar
Author Response
Dear Reviewer, Thank you so much.
Q1-
Line 2 - Hypericum salsolifolium should be wrtitten in italics
Ok
Line 3 – Please use superspcripts to identify the authors, please also remove orcid numbers from their affiliations
Ok
Line 9 – please expand all acronyms in Abstract
Ok
Line 31 and the rest of the manuscript – All the reference list and citation style must follow journal guidelines.
Ok
Line 56 – please present your hypothesis properly
Ok
Line 62 – please provide more details for all reagents used in the experiment (manufacturers’ codes, concentrations, etc.)
ok
Line 83 – Please change to “oC”
Ok
Line 85 – please explain what is TPC?
Total phenolic content
Line 132 – what was a source of cell lines ?
We used cell lines purchased from America Type Culture Collection (Manassas, VA) stored in liquid nitrogen.
Line 172 – what was post-hoc test used in ANOVA?
Duncan test
Figure 2 – please add scale bar
In Figure 2, the scala bar could not be shown because it was a morphologically visual microscopic image. Quantitative information is shown in Figure 3.
Round 2
Reviewer 2 Report
The paper has been modified and is in satisfactory state.
Please change the paper's title as it appears ambiguous.
Put an appropriate title as per the research work carried out.
Author Response
Dear Reviewer
Thank you.
We change the paper's title and put an appropriate title as per the research work carried out.
Reviewer 3 Report
Dear authors,
The comments from the discussion are poor in details and no references. It is necessary to improve the section and conclusions.
Best regards!
Only minor modifications observed.
Author Response
Dear Reviewer
thank you,
The introduction part was supported and the relevant references were added.
In the discussion part, information about phenolic substances and microbiota was given with references. Necessary changes were made in the Conclusions section.
Round 3
Reviewer 3 Report
Dear authors,
Several modifications should be made for figures, for example, figure 1 - A549 is not necessary, and all details in the figure legend; figure 3, 5 - the same comment; what means the table under figure 5? Is not necessary this format, figure or table for the same data; the number of references in the comments from the discussion is very low, it should increase with new relevant references at past comments.
Best regards!
Author Response
Dear reviewer
1-figure 1 removed from the article on your suggestion
2-The difference between figure 3 and figure 5 is that. In figure 3 we explain apoptotic cell death, in figure 5 we explain that the cell cycle stops in S phase
3-Upon your suggestion, the tables under figure 3 and figure 5 were removed.
4-Upon your suggestion, the references in the discussion section have been increased on the subject.
Best regards
