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Article
Peer-Review Record

Performance of the OncomineTM Lung cfDNA Assay for Liquid Biopsy by NGS of NSCLC Patients in Routine Laboratory Practice

Appl. Sci. 2020, 10(8), 2895; https://doi.org/10.3390/app10082895
by Giuseppa De Luca 1, Sonia Lastraioli 1, Romana Conte 1, Marco Mora 2, Carlo Genova 3, Giovanni Rossi 3, Marco Tagliamento 3, Simona Coco 3, Maria Giovanna Dal Bello 3, Simona Zupo 1 and Mariella Dono 1,*
Reviewer 1: Anonymous
Reviewer 2:
Reviewer 3: Anonymous
Appl. Sci. 2020, 10(8), 2895; https://doi.org/10.3390/app10082895
Submission received: 31 March 2020 / Revised: 18 April 2020 / Accepted: 20 April 2020 / Published: 22 April 2020
(This article belongs to the Special Issue Big Data Analytics for Cancer Research and Precision Medicine)

Round 1

Reviewer 1 Report

Beside the use of the reference DNA with serial dilutions of allele frequencies, it would have been also interesting to check a serial dilution of input amounts with defined reference DNA. This could help to gain information concerning the relation of input amounts and mutation detection. A statement on that is difficult when only using patient material because the real amount of ctDNA in the cfDNA is, as the authors mention, unclear.

Furthermore, repeated determination to determine the reproducibility or a comparison to another assay would have been of interest.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

The authors described the performance of the OLcfA quite well in the aspect of technology and the clinical aspect. And It would have been better to compare the tumor results. 

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 3 Report

This study highlights the use of cfDNA in lung cancer using an NGS approach with molecular barcodes - an important study in the field of liquid biopsy. 

Figure 1 - this has been screenshot and contains the error markings in red underneath - please correct.

3.2  - the plasma concentration range of 2.6ng/ml is below the minimum input of 20ng needed by the NGS platform? How have the authors addressed this? line 153 - 1ng but line 93 shows 20ng minimum?? which is it?

How has the data been validated? Has another approach been used?

line 355-359 - I think this speaks more to assay sensitivity and given that most samples were under the minimum input - what are the authors concerns here?

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 3 Report

The authors have now addressed my comments/concerns.

Author Response

We thank the Reviewer for suggestions.  

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