Serological and Molecular Survey on Domestic Dog Hepadnavirus in Household Dogs, Italy
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling
2.2. Expression of DCHSAg in the Baculovirus System and Development of Antibody Detection Enzyme-Linked Immunosorbent Assay (ELISA)
2.3. Serological Screening for Anti-DCHCAg and Anti-DCHSAg
2.4. Molecular Screening for Hepadnavirus
3. Results
3.1. Detection of Antibodies Anti-DCHCAg (DCHCAbs)
3.2. Molecular Detection and Sequence Analysis
3.3. Detection of Antibodies Anti-DCHSAg
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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DCHCAbs | Positive | Negative |
---|---|---|
IgM anti-DCHCAg | 13 (2.2%) | 587 (97.8%) |
IgG anti-DCHCAg | 45 (7.5%) | 555 (92.5%) |
IgM and IgG anti-DCHCAg | 2 (0.3%) | 598 (99.7%) |
Total | 60 (10.0%) | 540 (90.0%) |
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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Fruci, P.; Palombieri, A.; Sarchese, V.; Aste, G.; Friedrich, K.G.; Martella, V.; Di Martino, B.; Di Profio, F. Serological and Molecular Survey on Domestic Dog Hepadnavirus in Household Dogs, Italy. Animals 2023, 13, 729. https://doi.org/10.3390/ani13040729
Fruci P, Palombieri A, Sarchese V, Aste G, Friedrich KG, Martella V, Di Martino B, Di Profio F. Serological and Molecular Survey on Domestic Dog Hepadnavirus in Household Dogs, Italy. Animals. 2023; 13(4):729. https://doi.org/10.3390/ani13040729
Chicago/Turabian StyleFruci, Paola, Andrea Palombieri, Vittorio Sarchese, Giovanni Aste, Klaus G. Friedrich, Vito Martella, Barbara Di Martino, and Federica Di Profio. 2023. "Serological and Molecular Survey on Domestic Dog Hepadnavirus in Household Dogs, Italy" Animals 13, no. 4: 729. https://doi.org/10.3390/ani13040729