Outdoor Finishing of Intact Male Portuguese Alentejano Pigs on a Sustainable High-Fiber Diet: Impacts on Blood, Growth, Carcass, Meat Quality and Boar Taint Compounds
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Experimental Design
2.2. Serum Biochemistry
2.3. Carcass Traits and Tissue Sampling
2.4. Diets and Muscle Analyses
2.5. Calculations and Data Analyses
3. Results
3.1. Growth Data
3.2. Fasting Serum Parameters
3.3. Carcass Characteristics and Cut Proportions
3.4. Muscle Physical–Chemical Composition
3.5. Fatty Acids Profile of Muscle Tissues
3.6. Androstenone and Skatole Content in Neck Subcutaneous Fat Tissue
4. Discussion
4.1. Growth Data
4.2. Blood Parameters
4.3. Carcass Characteristics and Cut Proportions
4.4. Muscle Physical–Chemical Composition
4.5. Fatty Acids Profile of Muscle Tissues
4.6. Androstenone and Skatole in Fat Tissues
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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C | I | IExp | p-Value | ||||
---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | ||
Initial weight (kg) | 41.1 | 0.8 | 42.6 | 0.8 | 41.8 | 1.1 | 0.510 |
Final weight (kg) | 158.1 | 1.5 | 156.2 | 2.7 | 157.2 | 2.4 | 0.830 |
Total weight gain (kg) | 117.7 | 1.8 | 114.7 | 3.4 | 115.7 | 2.4 | 0.708 |
Days on trial (d) | 192.5 a | 4.7 | 175.3 b | 2.0 | 167.2 b | 4.0 | <0.001 |
Average total feed intake (ATFI) (kg) | 543.6 a | 3.8 | 479.6 b | 5.4 | 450.2 c | 3.0 | <0.0001 |
ATFI ~40–60 kg LW (kg) | 77.4 | 6.3 | 66.8 | 3.0 | 68.1 | 3.4 | 0.206 |
ATFI ~60–120 kg LW (kg) | 202.4 | 4.5 | 200.0 | 7.4 | 189.1 | 5.3 | 0.250 |
ATFI ~120–130 kg LW (kg) | 116.0 a | 7.6 | 53.9 b | 6.2 | 62.2 b | 10.8 | <0.0001 |
ATFI ~130–160 kg LW (kg) | 148.4 | 6.9 | 159.1 | 12.2 | 131.5 | 16.4 | 0.304 |
Average total feed rejection (ATFR) (kg) | 4.5 b | 1.6 | 26.4 a | 4.8 | 3.9 b | 1.6 | <0.0001 |
ATFR ~40–60 kg LW (kg) | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
ATFR ~60–120 kg LW (kg) | 0.31 | 0.27 | 4.88 | 2.74 | 0.65 | 0.26 | 0.097 |
ATFR ~120–130 kg LW (kg) | 0.35 b | 0.19 | 5.35 a | 1.90 | 0.46 b | 0.22 | 0.005 |
ATFR ~130–160 kg LW (kg) | 3.82 b | 1.38 | 16.20 a | 2.10 | 2.78 b | 1.63 | <0.0001 |
Feed conversion ratio (FCR) (kg/kg) | 4.63 a | 0.08 | 4.21 b | 0.13 | 3.90 c | 0.06 | <0.0001 |
FCR ~40–60 kg LW | 3.83 | 0.35 | 3.21 | 0.13 | 3.23 | 0.07 | 0.097 |
FCR ~60–120 kg LW | 3.94 a | 0.05 | 3.91 a | 0.11 | 3.61 b | 0.09 | 0.022 |
FCR ~120–130 kg LW | 5.32 a | 0.27 | 3.70 b | 0.32 | 4.03 b | 0.61 | 0.029 |
FCR ~130–160 kg LW | 6.74 | 0.21 | 6.42 | 0.72 | 5.33 | 0.32 | 0.119 |
Average daily gain (ADG) (g/d) | 609.6 b | 12.2 | 649.2 ab | 21.6 | 691.2 a | 15.4 | 0.008 |
ADG ~40–60 kg | 482.6 b | 12.9 | 558.5 a | 14.9 | 556.0 a | 12.6 | <0.001 |
ADG ~60–120 kg | 689.5 | 11.8 | 717.8 | 21.9 | 741.6 | 16.6 | 0.124 |
ADG ~120–130 kg | 686.5 b | 29.3 | 936.4 a | 80.8 | 935.7 a | 74.6 | 0.016 |
ADG ~130–160 kg | 591.1 b | 21.6 | 595.0 b | 53.5 | 732.2 a | 46.8 | 0.048 |
C | I | IExp | p-Value | ||||
---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | ||
Blood collected at ~120 kg LW | |||||||
Total protein (g/L) | 64.6 | 1.1 | 62.2 | 1.5 | 64.0 | 1.5 | 0.454 |
Urea (mmol/L) | 4.96 a | 0.13 | 4.05 b | 0.22 | 3.33 c | 0.16 | <0.0001 |
Glucose (mmol/L) | 4.07 | 0.11 | 4.35 | 0.15 | 4.26 | 0.15 | 0.366 |
Triacylglycerols (mmol/L) | 0.28 | 0.01 | 0.29 | 0.02 | 0.33 | 0.02 | 0.163 |
Total cholesterol (mmol/L) | 2.49 | 0.08 | 2.17 | 0.08 | 2.47 | 0.20 | 0.215 |
Cortisol (nmol/L) † | 177.2 | 54.8 | 125.4 | 18.1 | 127.3 | 12.6 | 0.382 |
Testosterone (nmol/L) † | 0.21 c | 0.03 | 10.70 b | 0.89 | 19.03 a | 2.88 | <0.0001 |
Blood collected at ~160 kg LW | |||||||
Total protein (g/L) | 65.3 | 1.6 | 64.3 | 1.8 | 66.2 | 2.7 | 0.809 |
Urea (mmol/L) | 4.01 a | 0.21 | 3.36 ab | 0.30 | 3.11 b | 0.17 | 0.034 |
Glucose (mmol/L) | 4.12 | 0.11 | 4.20 | 0.12 | 4.15 | 0.10 | 0.876 |
Triacylglycerols (mmol/L) | 0.53 a | 0.04 | 0.37 b | 0.04 | 0.30 b | 0.03 | <0.001 |
Total cholesterol (mmol/L) | 2.41 | 0.12 | 2.12 | 0.21 | 2.10 | 0.19 | 0.418 |
Cortisol (nmol/L) † | 204.4 a | 22.4 | 232.0 a | 31.4 | 78.9 b | 7.8 | <0.001 |
Testosterone (nmol/L) † | 0.26 c | 0.03 | 16.34 b | 2.98 | 25.02 a | 2.34 | <0.0001 |
C | I | IExp | p-Value | ||||
---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | ||
Hot carcass weight (kg) | 124.7 | 1.3 | 117.7 | 2.2 | 121.9 | 2.1 | 0.051 |
Carcass yield (%) | 78.8 a | 0.2 | 75.4 c | 0.5 | 77.6 b | 0.2 | <0.001 |
Commercial yield (%) 1 | 46.6 b | 0.5 | 48.8 a | 0.3 | 48.9 a | 0.3 | <0.001 |
Untrimmed shoulder (%) | 17.1 b | 0.3 | 19.4 a | 0.3 | 20.1 a | 0.2 | <0.0001 |
Loin (%) | 4.59 | 0.13 | 4.91 | 0.08 | 4.53 | 0.14 | 0.068 |
Untrimmed ham (%) | 24.5 | 0.4 | 24.0 | 0.1 | 23.7 | 0.3 | 0.168 |
Tenderloin (%) | 0.49 b | 0.01 | 0.57 a | 0.02 | 0.52 ab | 0.02 | 0.028 |
Primal cuts (%) 2 | 46.1 b | 0.5 | 48.3 a | 0.3 | 48.4 a | 0.3 | <0.001 |
Bone cuts (%) 3 | 7.7 b | 0.2 | 9.0 a | 0.2 | 8.7 a | 0.2 | 0.011 |
Neck (bone-in; %) | 4.05 b | 0.16 | 4.96 a | 0.20 | 4.98 a | 0.17 | 0.001 |
Ribs (%) | 3.65 | 0.13 | 4.00 | 0.17 | 3.72 | 0.08 | 0.145 |
Fat cuts (%) 4 | 28.7 a | 0.3 | 25.0 b | 0.4 | 24.7 b | 0.4 | <0.0001 |
Belly (%) | 12.6 a | 0.4 | 10.0 b | 0.4 | 10.9 b | 0.4 | <0.001 |
Backfat (%) | 16.1 a | 0.4 | 15.0 a | 0.3 | 13.8 b | 0.5 | 0.002 |
Lean to fat cuts ratio | 1.63 b | 0.03 | 1.96 a | 0.04 | 1.98 a | 0.04 | <0.0001 |
Lean to bone cuts ratio | 6.1 a | 0.2 | 5.5 b | 0.1 | 5.7 b | 0.1 | 0.024 |
Backfat thickness (mm) 5 | 68.2 a | 1.1 | 47.8 b | 3.0 | 50.5 b | 2.4 | <0.0001 |
10th rib backfat thickness | 72.8 a | 1.7 | 50.2 b | 2.8 | 52.4 b | 2.4 | <0.0001 |
Last rib backfat thickness | 63.7 a | 1.1 | 45.3 b | 3.3 | 48.5 b | 2.6 | <0.0001 |
Fatness: ZP fat depth (mm) 6 | 62.7 a | 1.9 | 46.4 b | 2.2 | 48.6 b | 1.4 | <0.0001 |
Last rib LL thickness (mm) | 54.8 | 1.5 | 60.1 | 2.0 | 57.8 | 1.5 | 0.102 |
Leanness: ZP muscle depth (mm) 7 | 64.4 | 1.1 | 67.8 | 1.3 | 64.7 | 1.1 | 0.092 |
C | I | IExp | p-Value | ||||
---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | ||
Moisture (g/100 g) | 71.4 b | 0.2 | 73.5 a | 0.3 | 73.0 a | 0.2 | <0.0001 |
Total protein (g/100 g) | 22.3 | 0.4 | 21.8 | 0.2 | 21.5 | 0.6 | 0.503 |
Total intramuscular fat (g/100 g) | 4.12 a | 0.36 | 2.72 b | 0.11 | 2.96 b | 0.31 | 0.004 |
Total ashes (g/100 g) | 1.17 | 0.03 | 1.11 | 0.01 | 1.14 | 0.03 | 0.174 |
pH (24 h post mortem) | 5.68 | 0.04 | 5.60 | 0.02 | 5.61 | 0.03 | 0.064 |
Drip loss (g/100 g) | 0.27 | 0.03 | 0.53 | 0.15 | 0.46 | 0.11 | 0.212 |
Thawing loss (g/100 g) | 1.57 | 0.24 | 0.94 | 0.18 | 1.09 | 0.18 | 0.100 |
Cooking loss (g/100 g) | 14.8 | 0.5 | 15.0 | 0.3 | 14.2 | 0.8 | 0.535 |
Myoglobin content (mg/g) | 1.41 | 0.09 | 1.24 | 0.06 | 1.25 | 0.08 | 0.229 |
Lightness (CIE L*) | 44.3 | 0.8 | 45.7 | 1.0 | 46.9 | 0.7 | 0.125 |
Redness (CIE a*) | 13.5 | 0.6 | 13.0 | 0.7 | 12.2 | 0.4 | 0.254 |
Yellowness (CIE b*) | 9.1 | 0.6 | 9.3 | 0.7 | 9.1 | 0.3 | 0.926 |
Chroma (C) | 16.3 | 0.8 | 16.0 | 0.9 | 15.2 | 0.4 | 0.566 |
Hue angle (H°) | 33.6 | 1.1 | 35.3 | 1.1 | 36.7 | 0.8 | 0.111 |
Saturation | 0.37 | 0.02 | 0.35 | 0.02 | 0.33 | 0.01 | 0.169 |
Total collagen (mg/g DM) | 17.9 b | 0.5 | 20.6 a | 0.6 | 20.4 a | 0.5 | 0.002 |
Soluble collagen (mg/g DM) | 5.9 | 0.3 | 6.8 | 0.3 | 6.6 | 0.4 | 0.110 |
Warner–Bratzler shear force (N) | 48.7 | 2.7 | 52.7 | 2.5 | 47.2 | 1.3 | 0.182 |
C | I | IExp | p-Value | ||||
---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | ||
g/100 g of Total Fatty Acids Identified | |||||||
C14 | 1.25 a | 0.02 | 1.05 c | 0.02 | 1.18 b | 0.03 | <0.0001 |
C16 | 25.3 a | 0.2 | 23.6 b | 0.2 | 24.1 b | 0.2 | <0.0001 |
C18 | 10.2 | 0.2 | 9.9 | 0.3 | 10.1 | 0.2 | 0.620 |
C20 | 0.12 | 0.01 | 0.12 | 0.01 | 0.11 | 0.01 | 0.280 |
ΣSFA | 37.2 a | 0.4 | 35.0 b | 0.4 | 35.9 b | 0.4 | 0.001 |
C16:1 n-7 | 3.47 a | 0.09 | 3.04 b | 0.13 | 3.10 b | 0.12 | 0.023 |
C16:1 n-9 | 0.25 b | 0.01 | 0.38 a | 0.02 | 0.35 a | 0.02 | <0.0001 |
C18:1 n-7 | 3.97 a | 0.08 | 3.67 b | 0.13 | 3.57 b | 0.08 | 0.018 |
C18:1 n-9 | 47.7 | 0.3 | 47.0 | 0.3 | 46.6 | 0.5 | 0.196 |
ΣMUFA | 55.5 a | 0.3 | 54.3 ab | 0.5 | 53.8 b | 0.6 | 0.034 |
C18:2 n-6 | 5.1 b | 0.1 | 8.0 a | 0.3 | 7.8 a | 0.3 | <0.0001 |
C18:3 n-3 | 0.87 b | 0.02 | 0.98 a | 0.01 | 0.98 a | 0.02 | <0.001 |
C20:2 n-6 | 0.15 b | 0.01 | 0.22 a | 0.01 | 0.22 a | 0.01 | <0.0001 |
C20:4 n-6 | 0.73 | 0.05 | 1.00 | 0.10 | 0.82 | 0.11 | 0.110 |
C20:5 n-3 | 0.029 | 0.003 | 0.044 | 0.006 | 0.035 | 0.005 | 0.099 |
C22:5 n-3 | 0.070 | 0.008 | 0.079 | 0.006 | 0.077 | 0.10 | 0.703 |
C22:6 n-3 | 0.008 | 0.002 | 0.006 | 0.003 | 0.003 | 0.002 | 0.407 |
ΣPUFA | 7.3 b | 0.2 | 10.8 a | 0.4 | 10.3 a | 0.4 | <0.0001 |
ΣUFA | 62.8 b | 0.4 | 65.0 a | 0.4 | 64.1 a | 0.4 | 0.001 |
ΣUFA/SFA | 1.69 b | 0.03 | 1.87 a | 0.04 | 1.79 a | 0.03 | 0.002 |
ΣPUFA/SFA | 0.20 b | 0.01 | 0.31 a | 0.01 | 0.29 a | 0.01 | <0.0001 |
Σn-3 | 1.03 b | 0.03 | 1.18 a | 0.02 | 1.16 a | 0.02 | <0.0001 |
Σn-6 | 6.2 b | 0.2 | 9.5 a | 0.4 | 9.1 a | 0.4 | <0.0001 |
Σn-3/n-6 | 0.17 a | 0.01 | 0.13 b | 0.01 | 0.13 b | 0.01 | <0.0001 |
Σn-6/n-3 | 6.0 b | 0.2 | 8.1 a | 0.3 | 7.9 a | 0.3 | <0.0001 |
Σn-9 | 48.0 | 0.3 | 47.4 | 0.4 | 47.0 | 0.5 | 0.262 |
SAT index † | 0.59 a | 0.01 | 0.53 b | 0.01 | 0.55 b | 0.01 | 0.001 |
ATH index ‡ | 0.49 a | 0.01 | 0.43 c | 0.01 | 0.45 b | 0.01 | <0.0001 |
C | I | IExp | p-Value | ||||
---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | ||
Androstenone (ng/g) | 28.4 b | 17.1 | 669.5 a | 89.9 | 725.0 a | 98.4 | <0.0001 |
Skatole (ng/g) | 1.08 | 0.00 | 2.34 | 1.26 | 7.56 | 6.48 | 0.454 |
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Martins, J.M.; Varino, R.; Charneca, R.; Albuquerque, A.; Garrido, N.; Neves, J.; Freitas, A.; Costa, F.; Marmelo, C.; Ramos, A.; et al. Outdoor Finishing of Intact Male Portuguese Alentejano Pigs on a Sustainable High-Fiber Diet: Impacts on Blood, Growth, Carcass, Meat Quality and Boar Taint Compounds. Animals 2023, 13, 2221. https://doi.org/10.3390/ani13132221
Martins JM, Varino R, Charneca R, Albuquerque A, Garrido N, Neves J, Freitas A, Costa F, Marmelo C, Ramos A, et al. Outdoor Finishing of Intact Male Portuguese Alentejano Pigs on a Sustainable High-Fiber Diet: Impacts on Blood, Growth, Carcass, Meat Quality and Boar Taint Compounds. Animals. 2023; 13(13):2221. https://doi.org/10.3390/ani13132221
Chicago/Turabian StyleMartins, José Manuel, Ricardo Varino, Rui Charneca, André Albuquerque, Nicolás Garrido, José Neves, Amadeu Freitas, Filipa Costa, Carla Marmelo, Amélia Ramos, and et al. 2023. "Outdoor Finishing of Intact Male Portuguese Alentejano Pigs on a Sustainable High-Fiber Diet: Impacts on Blood, Growth, Carcass, Meat Quality and Boar Taint Compounds" Animals 13, no. 13: 2221. https://doi.org/10.3390/ani13132221