Effect of Environmental Concentration of Carbamazepine on the Behaviour and Gene Expression of Laboratory Rats
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Treatment
2.2. Behavioural Tests
2.2.1. Elevated plus Maze
2.2.2. Novel Object Recognition Task
2.2.3. Social Preference Test
First Phase: “Sociability”
Second Phase: “Preference of Social Novelty”
2.3. Euthanasia of Animals and Sampling
2.4. Primer Design, Isolation of RNA, and qPCR
Gene | Primers | Primer Sequences (5′–3′) | Amplicon (bp) | Ta 1 (°C) | R2 2 | PCR Efficiency (%) | Reference |
---|---|---|---|---|---|---|---|
Actb | F | ATCGCTGACAGGATGCAGAAG | 108 | 62 | 0.996 | 102.5 | [49] |
R | AGAGCCACCAATCCACACAGA | ||||||
Gadph | F | AGGGCTGCCTTCTCTTGTGAC | 101 | 58 | 0.997 | 99.4 | [49] |
R | TGGGTAGAATCATACTGGAACATGTAG | ||||||
Ppia | F | AAGGTGAAAGAAGGCATGAG | 76 | 56 | 0.997 | 101.2 | [50] |
R | CCGCAAGTCAAAGAAATTAGAG | ||||||
Ugt1a6 | F | GGGAGAATCCAAATACTACAGGAG | 100 | 60 | 0.999 | 101.8 | [53] |
R | CAGCAAAGTGGTTGTTCCCAAAGG | ||||||
Ugt1a7 | F | CAGACCCCGGTGACTATGACA | 73 | 61 | 0.997 | 97.7 | [53] |
R | CAACGTGAAGTCTGTGCGTAACA |
Statistical Analyses
3. Results
3.1. Elevated plus Maze
3.2. Novel Object Recognition Test
3.3. Social Chamber Test
3.4. The Effect of CBZ on Gene Expression
4. Discussion
4.1. Elevated plus Maze Anxiety Test
4.2. Novel Object Recognition Test
4.3. Social Preference Test
4.4. Effect of CBZ on Gene Expression in Rat Brain Tissue
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Bialer, M. Chemical properties of antiepileptic drugs (AEDs). Adv. Drug Deliv. Rev. 2012, 64, 887–895. [Google Scholar] [CrossRef]
- RxList. Available online: http://www.rxlist.com/tegretol-drug.htm (accessed on 19 September 2022).
- Lingamaneni, R.; Hemmings, H.C. Effects of anticonvulsants on veratridine and KCL evoked glutamate release from rat cortical synaptosomes. Neurosci. Let. 1999, 276, 127–130. [Google Scholar] [CrossRef] [PubMed]
- Mula, M.; Pini, S.; Cassano, G. The role of anticonvulsant drugs in anxiety disorders: A critical review of the evidence. J. Clin. Psychopharmacol. 2007, 27, 263–272. [Google Scholar] [CrossRef]
- Afeltowicz, Z.; Majkowicz, M.; Michalik, K. Carbamazepine in the treatment in psychiatric disorders based on retrospective clinical study. Psychiatr. Pol. 2002, 36 (Suppl. S6), 303–310. [Google Scholar] [PubMed]
- Ceron-Litvoc, D.; Soares, B.G.; Geddes, J.; Litvoc, J.; Lima, M.S.D. Comparison of carbamazepine and lithium in treatment of bipolar disorder: A systematic review of randomized controlled trials. Hum. Psychopharmacol. 2009, 24, 19–28. [Google Scholar] [CrossRef]
- Rezvanfard, M.; Zarrindast, M.R.; Bina, P. Role of ventral hippocampal GABAA and NMDA receptors in the anxiolytic effect of carbamazepine in rats using the Elevated Plus Maze test. Pharmacology 2009, 84, 356–366. [Google Scholar] [CrossRef]
- Yassa, R.; Dupont, D. Carbamazepine in the Treatment of Aggressive Behaviour in Schizophrenic Patients: A Case Report. Can. J. Psychiatry 1983, 28, 566–568. [Google Scholar] [CrossRef]
- Zhang, Y.; Geißen, S.; Gal, C. Carbamazepine and diclofenac: Removal in wastewater treatment plants and occurrence in water bodies. Chemosphere 2008, 73, 1151–1161. [Google Scholar] [CrossRef] [PubMed]
- Ternes, T.A. Occurence of drugs in German sewage treatment plants and rivers. Water Res. 1998, 32, 3245–3260. [Google Scholar] [CrossRef]
- Clara, M.; Strenn, B.; Kreuzinger, N. Carbamazepine as a possible anthropogenic marker in the aquatic environment: Investigations on the behaviour of carbamazepine in wastewater treatment and during groundwater infiltration. Water Res. 2004, 38, 947–954. [Google Scholar] [CrossRef]
- Gebhardt, W.; Schröder, H.F. Liquid chromatography-(tandem) mass spectrometry for the follow-up of the elimination of persistent pharmaceuticals during wastewater treatment applying biological wastewater treatment and advanced oxidation. J. Chromatogr. A 2007, 1160, 34–43. [Google Scholar] [CrossRef]
- Khetan, S.K.; Collins, T.J. Human pharmaceuticals in the aquatic environment: A challenge to green chemistry. Chem. Rev. 2007, 107, 2319–2364. [Google Scholar] [CrossRef]
- Fent, K.; Weston, A.A.; Caminada, D. Ecotoxicology of human pharmaceuticals. Aquat. Toxicol. 2006, 76, 122–159. [Google Scholar] [CrossRef]
- Klosterhaus, S.L.; Grace, R.; Hamilton, M.C.; Yee, D. Method validation and reconnaissance of pharmaceuticals, personal care products, and alkylphenols in surface waters, sediments, and mussels in an urban estuary. Environ. Int. 2013, 54, 92–99. [Google Scholar] [CrossRef] [PubMed]
- McEneff, G.; Barron, L.; Kelleher, B.; Paull, B.; Quinn, B. A year-long study of the spatial occurrence and relative distribution of pharmaceutical residues in sewage effluent, receiving marine waters and marine bivalves. Sci. Total Environ. 2014, 476, 317–326. [Google Scholar] [CrossRef]
- Herklotz, P.A.; Gurung, P.; Heuvel, B.V.; Kinney, C.A. Uptake of human pharmaceuticals by plants grown under hydroponic conditions. Chemosphere 2010, 78, 1416–1421. [Google Scholar] [CrossRef] [PubMed]
- Hurtado, C.; Domínguez, C.; Pérez-Babace, L.; Cañameras, N.; Comas, J.; Bayona, J.M. Estimate of uptake and translocation of emerging organic contaminants from irrigation water concentration in lettuce grown under controlled conditions. J. Hazard. Mater. 2016, 305, 139–148. [Google Scholar] [CrossRef]
- Wu, X.; Ernst, F.; Conkle, J.L.; Gan, J. Comparative uptake and translocation of pharmaceutical and personal care products (PPCPs) by common vegetables. Environ Int. 2013, 60, 15–22. [Google Scholar] [CrossRef] [PubMed]
- Pomati, F.; Castiglioni, S.; Zuccato, E.; Fanelli, R.; Vigetti, D.; Rossetti, C.; Calamari, D. Effects of a complex mixture of therapeutic drugs at environmental levels on human embryonic cells. Environ. Sci. Technol. 2006, 40, 2442–2447. [Google Scholar] [CrossRef]
- Calcagno, E.; Durando, P.; Valdés, M.E.; Franchioni, L.; de los Ángeles Bistoni, M. Effects of carbamazepine on cortisol levels and behavioural responses to stress in the fish Jenynsia multidentata. Physiol. Behav. 2016, 158, 68–75. [Google Scholar] [CrossRef]
- Nassef, M.; Matsumoto, S.; Seki, M.; Khalil, F.; Kang, I.J.; Shimasaki, Y.; Honjo, T. Acute effects of triclosan, diclofenac and carbamazepine on feeding performance of Japanese medaka fish (Oryzias latipes). Chemosphere 2010, 80, 1095–1100. [Google Scholar] [CrossRef] [PubMed]
- Nadeem, A.; Ahmad, S.F.; Al-Harbi, N.O.; Attia, S.M.; Bakheet, S.A.; Alsanea, S.; Alsaleh, N.B. Aggravation of autism-like behavior in BTBR T+ tf/J mice by environmental pollutant, di-(2-ethylhexyl) phthalate: Role of nuclear factor erythroid 2-related factor 2 and oxidative enzymes in innate immune cells and cerebellum. Int. Immunopharmacol. 2021, 91, 107323. [Google Scholar] [CrossRef]
- Albano, M.; Panarello, G.; Di Paola, D.; Capparucci, F.; Crupi, R.; Gugliandolo, E.; Spanò, N.; Capillo, G.; Savoca, S. The Influence of Polystyrene Microspheres Abundance on Development and Feeding Behavior of Artemia salina (Linnaeus, 1758). Appl. Sci. 2021, 11, 3352. [Google Scholar] [CrossRef]
- Qiang, L.; Cheng, J. Environmental concentration of carbamazepine accelerates fish embryonic development and disturbs larvae behaviour. Ecotoxicology 2016, 25, 1426–1437. [Google Scholar] [CrossRef]
- Rostock, A.; Hoffmann, W.; Siegemund, C. Effects of carbamazepine, valproate calcium, clonazepam and piracetam on behavioural test methods for evaluation of memory-enhancing drugs. Methods Find Exp. Clin. Pharmacol. 1989, 11, 547–553. [Google Scholar] [PubMed]
- Bernardi, R.B.; Barros, H.M. Carbamazepine enhances discriminative memory in a rat model of epilepsy. Epilepsia 2004, 45, 1443–1447. [Google Scholar] [CrossRef] [PubMed]
- Nowakowska, E.; Kus, K.; Czubak, A.; Glowacka, D.; Matschay, A. Some behavioural effects of carbamazepine-comparison with haloperidol. J. Physiol. Pharmacol. 2007, 58, 253. [Google Scholar]
- Martijena, I.D.; Lacerra, C.; Molina, V.A. Carbamazepine normalizes the altered behavioural and neurochemical response to stress in benzodiazepine-withdrawn rats. Eur. J. Pharmacol. 1997, 330, 101–108. [Google Scholar] [CrossRef]
- Okon, U.E.; Erigbali, P.P.; Osim, E.E. Comparative effects of antiepileptic agents Dichrostachys glomerata ethanol extract and carbamazepine on seizures and anxiety in mice. J. Adv. Med. Med. Res. 2017, 24, 1–10. [Google Scholar] [CrossRef]
- Zangrossi, H.; Leite, J.R.; Graeff, F.G. Anxiolytic effect of carbamazepine in the elevated plus-maze: Possible role of adenosine. Psychopharmacology 1992, 106, 85–89. [Google Scholar] [CrossRef]
- Reinvang, I.; Bjartveit, S.; Johannessen, O.P. Cognitive function and time-of-day variation in serum carbamazepine concentration in epileptic patients treated with monotherapy. Epilepsia 1991, 32, 116–121. [Google Scholar] [CrossRef]
- Sudha, S.; Lakshmana, M.K.; Pradhan, N. Changes in learning and memory, acetylcholinesterase activity and monoamines in brain after chronic carbamazepine administration. Epilepsia 1995, 36, 416–422. [Google Scholar] [CrossRef] [PubMed]
- Trimble, M.R. Anticonvulsant drugs and cognitive function: Review of the literature. Epilepsia 1988, 28 (Suppl. S3), 37–45. [Google Scholar] [CrossRef]
- Li, L.; Zhang, S.; Zhang, X.; Li, T.; Tang, Y.; Liu, H.; Le, W. Autophagy enhancer carbamazepine alleviates memory deficits and cerebral amyloid-β pathology in a mouse model of Alzheimer’s disease. Curr. Alzheimer Res. 2013, 10, 433–441. [Google Scholar] [CrossRef] [PubMed]
- Azouvi, P.; Jokic, C.; Attal, N.; Pierre, D.; Sabria, M.; Bussel, B. Carbamazepine in agitation and aggressive behaviour following severe closed-head injury: Results of an open trial. Brain Inj. 1999, 13, 797–804. [Google Scholar] [CrossRef] [PubMed]
- Sakakibara, Y.; Katoh, M.; Kondo, Y.; Nadai, M. Effects of Phenobarbital on Expression of UDP-Glucuronosyltransferase 1a6 and 1a7 in Rat Brain. Drug Metab. Dispos. 2016, 44, 370–377. [Google Scholar] [CrossRef] [Green Version]
- Allain, E.P.; Rouleau, M.; Levasque, E.; Guillemette, C. Emerging roles for UDP-glucuronosyltransferases in drug resistance and cancer progression. Br. J. Cancer 2020, 122, 1277–1287. [Google Scholar] [CrossRef] [Green Version]
- Tirona, R.G.; Kim, R.B. Nuclear receptors and drug disposition gene regulation. J. Pharm. Sci. 2005, 94, 1169–1186. [Google Scholar] [CrossRef]
- Mazerska, Z.; Mróz, A.; Pawlowska, M.; Augustin, E. The role of glucuronidation in drug resistence. Pharmacol. Ther. 2016, 159, 35–55. [Google Scholar] [CrossRef]
- Oscarson, M.; Zanger, U.M.; Rifki, O.F.; Klein, K.; Eichelbaum, M.; Meyer, U.A. Transcriptional profiling of genes induced in the livers of patients treated with carbamazepine. Clin. Pharmacol. Ther. 2006, 80, 440–456. [Google Scholar] [CrossRef]
- Kutsuno, Y.; Hirashima, R.; Sakamoto, M.; Ushikubo, H.; Michimae, H.; Itoh, T. Expression of UDP-glucuronosyltransferase 1 (UGT1) and glucuronidation activity toward endogenous substances in humanized UGT1 mouse brain. Drug Metab. Dispos. 2015, 43, 1071–1076. [Google Scholar] [CrossRef] [Green Version]
- Braun, A.A.; Skelton, M.R.; Vorhees, C.V.; Williams, M.T. Comparison of the elevated plus and elevated zero mazes in treated and untreated male Sprague–Dawley rats: Effects of anxiolytic and anxiogenic agents. Pharmacol. Biochemi. Behav. 2011, 97, 406–415. [Google Scholar] [CrossRef] [Green Version]
- Burke, S.N.; Wallace, J.L.; Nematollahi, S.; Uprety, A.R.; Barnes, C.A. Pattern separation deficits may contribute to age-associated recognition impairments. Behav. Neurosci. 2010, 124, 559–573. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Oliveira, A.M.M.; Hawk, J.D.; Abel, T.; Havekes, R. Post-training reversible inactivation of the hippocampus enhances novel object recognition memory. Learn Mem. 2010, 17, 155–160. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Silvers, J.M.; Harrod, S.B.; Mactutus, C.F.; Booze, R.M. Automation of the novel object recognition task for use in adolescent rats. J. Neurosci. Met. 2007, 166, 99–103. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Botton, P.H.; Costa, M.S.; Ardais, A.P.; Mioranzza, S.; Souza, D.O.; da Rocha, J.B.; Porciu´ncula, L.O. Caffeine prevents disruption of memory consolidation in the inhibitory avoidance and novel object recognition tasks by scopolamine in adult mice. Behav. Brain. Res. 2010, 214, 254–259. [Google Scholar] [CrossRef]
- Gaskin, S.; Tardif, M.; Cole, E.; Piterkin, P.; Kayello, L.; Mumby, D.G. Object familiarization and novel-object preference in rats. Behav. Proc. 2010, 83, 61–71. [Google Scholar] [CrossRef]
- Chen, J.; Rider, D.A.; Ruan, R. Identification of Valid Housekeeping Genes and Antiioxidant Enzymw Gene Expression Change in the Aging Rat Liver. J. Gerontol. Ser. A Biol. Sci. Med. Sci. 2006, 61, 20–27. [Google Scholar] [CrossRef] [Green Version]
- Feria-Romero, I.A.; Bribiesca-Cruz, I.; Coyoy-Salgado, A.; Segura-Uribe, J.J.; Bautista-Poblet, G.; Granados-Cervantes, A.; Guerra-Araiza, C. Validation of housekeeping genes as an internal control for gene expression studies in the brain of ovariectomized rats treated with tibolone. Gene 2021, 769, 145255. [Google Scholar] [CrossRef]
- Zadinová, K.; Stratil, A.; Van Poucke, M.; Peelman, L.; Čítek, J.; Okrouhlá, M.; Lebedová, N.; Pokorná, K.; Šprysl, M.; Stupka, R. Effect of dietary suppleementation with dried tuber of Jerusalem artichoke on skatole level in backfat and CYP2E1 mRNA expression in liver of boars. Ann. Anim. Sci. 2021, 4, 1475–1489. [Google Scholar] [CrossRef]
- Bustin, S.A.; Benes, V.; Garson, J.A.; Hellemans, J.; Huggett, J.; Kubista, M.; Mueller, R.; Nolan, T.; Pfaffl, M.W.; Shipley, G.L.; et al. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 2009, 55, 611–622. [Google Scholar] [CrossRef] [Green Version]
- Asai, Y.; Sakakibara, Y.; Nadai, M.; Katoh, M. Effect of carbamazepine on expression of UDP-glucuronosyltransferase 1A6 and 1A7 in rat brain. Drug Metab. Pharm. 2017, 32, 286–292. [Google Scholar] [CrossRef] [PubMed]
- Tukel, R. The efficacy and role of anticonvulsants as a treatment option in anxiety disorders/Anksiyete bozukluklarinda antikonvulzanlarin etkinligi ve bir tedavi secenegi olarak yeri. Arch. Neuropsychyatry 2009, 46, 19–24. [Google Scholar]
- Barnett, S.A. The Rat—A Study in Behaviour; University Chicago Press: Chicago, IL, USA, 1975. [Google Scholar]
- Pellow, S.; Chopin, P.; File, S.; Briley, M. Validation of open: Closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J. Neurosci. Methods 1985, 14, 149–167. [Google Scholar] [CrossRef] [PubMed]
- Lynch, J.J.; McCarthy, J.F. The effect of petting on a classically conditioned emotional response. Behav. Res. Ther. 1967, 5, 55–62. [Google Scholar] [CrossRef] [PubMed]
- Costa, R.; Tamascia, M.L.; Nogueira, M.D.; Casarini, D.E.; Marcondes, F.K. Handling of adolescent rats improves learning and memory and decreases anxiety. J. Am. Assoc. Lab. Anim. Sci. 2012, 51, 548–553. [Google Scholar] [PubMed]
- Smith, D.B.; Mattson, R.H.; Cramer, J.A.; Collins, J.F.; Novelly, R.A.; Craft, B.; Veterans Administration Epilepsy Cooperative Study Group. Results of a nationwide Veterans Administration Cooperative Study comparing the efficacy and toxicity of carbamazepine, phenobarbital, phenytoin, and primidone. Epilepsia 1987, 28, 50–58. [Google Scholar] [CrossRef]
- Meador, K.J.; Loring, D.W.; Ray, P.G.; Murro, A.M.; King, D.W.; Nichols, M.E.; Goff, W.T. Differential cognitive effects of carbamazepine and gabapentin. Epilepsia 1999, 40, 1279–1285. [Google Scholar] [CrossRef] [PubMed]
- Hawkins, C.A.; Mellanby, J.; Brown, J. Antiepileptic effect of carbamazepine in experimental limbic epilepsy. J. Neurol. Neurosurg. Psychiatry 1985, 48, 459–468. [Google Scholar] [CrossRef] [Green Version]
- Ennaceur, A. One-trial object recognition in rats and mice: Methodological and theoretical issues. Behav. Brain. Res. 2010, 215, 244–254. [Google Scholar] [CrossRef]
- Ennaceur, A.; Delacour, J. A new one-trial test for neurobiological studies of memory in rats. 1. Behavioural data. Behav. Brain Res. 1988, 31, 47–59. [Google Scholar] [CrossRef]
- Petr, T. Locomotor, emotional and cognitive skills of laboratory Norway rats with different early ontogenetic experience with social play [In Czech with English summary]. MSC Thesis, Univerzita Karlova, Prague, Czech Republic, 2022. [Google Scholar]
- Pulliainen, V.; Jokelainen, M. Effects of phenytoin and carbamazepine on cognitive functions in newly diagnosed epileptic patients. Acta. Neurol. Scand. 1994, 89, 81–86. [Google Scholar] [CrossRef]
- Calhoun, J.B. The Ecology and Sociology of the Norway Rat (No. 1008); US Department of Health Education and Welfare Public Health Service: Bethesda, MD, USA, 1963.
- Brodkin, E.S.; Hagemann, A.; Nemetski, S.M.; Silver, L.M. Social approach-avoidance behaviour of inbred mouse strains to DBA/2 mice. Brain. Res. 2004, 1002, 151–157. [Google Scholar] [CrossRef] [PubMed]
- Moy, S.S.; Nadler, J.J.; Young, N.B.; Nonneman, R.J.; Grossman, A.W.; Murphy, D.L. Social approach in genetically engineered mouse lines relevant to autism. Genes Brain Behav. 2009, 8, 129–142. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Nadler, J.J.; Moy, S.S.; Dold, G.; Trang, D.; Simmons, N.; Perez, A. Automated apparatus for rapid quantification of autism-like social deficits in mice. Genes Brain. Behav. 2004, 3, 303–314. [Google Scholar] [CrossRef]
- De Bruin, N.; Pouzet, B. Beneficial effects of galantamine on performance in the object recognition task in Swiss mice: Deficits induced by scopolamine and by prolonging the retention interval. Pharmacol. Biochem. Behav. 2006, 85, 253–260. [Google Scholar] [CrossRef]
- Chindo, B.A.; Howes, M.J.R.; Abuhamad, S.; Yakubu, M.I.; Ayuba, G.I.; Battison, A.; Chazot, P.L. New Insights Into the Anticonvulsant Effects of Essential Oil From Melissa officinalis L. (Lemon Balm). Front. Pharmacol. 2021, 12, 760674. [Google Scholar] [CrossRef] [PubMed]
- Boguszewski, P.; Zagrodzka, J. Emotional changes related to age in rats—A behavioral analysis. Behav. Brain Res. 2002, 133, 323–332. [Google Scholar] [CrossRef]
- Buragli, P.; Vitale, V.; Banti, L.; Sighieri, C. Effect of aging on behavioral and physiological responses to stressful stimulus in horses. Behaviour 2014, 151, 1513–1533. [Google Scholar] [CrossRef]
- Núñez, J.F.; Ferré, P.; Escorihuela, R.M.; Tobeña, A.; Fernández-Teruel, A. Effects of postnatal handling of rats on emotional, HPA-axis, and prolactin reactivity to novelty and conflict. Physiol. Behav. 1996, 60, 1355–1359. [Google Scholar] [CrossRef]
Variables | Definition |
---|---|
e1 | time spent in the zone of both objects in (phase 0) training session (5 min) |
e2 | time spent in the zone of both objects (new old) in (phase I) choice session (5 min) |
Teo | exploration time of the initial object in (phase I) choice session (5 min) |
Ten | exploration time of the new object in (phase I) choice session (5 min) |
VISo | number of visits to the starting object in phase I choice session (5 min) |
VISn | number of visits to the new object in phase I choice session (5 min) |
NOR_ih | habituation index of exploratory behaviour (phase 0) training session vs. (I) choice session NOR_ih = e1 − e2 |
NOR_D1 | discrimination index between new and familiar object NOR_D1 = TZn − Tzo |
NOR_DI | discrimination index between new and familiar object NOR_DI = D1/e2 |
NOR_RI | recognition index NOR_RI = TZn/e2 |
NOR_VI | visit index (new vs. familiar object) NOR_VI = VISn/VISn + VISo |
Item | Therapeutic | Environmental | Control | p-Value |
---|---|---|---|---|
OAE 1 | 0.317 ± 0.053 | 0.363 ± 0.054 | 0.325 ± 0.053 | Non-sign. |
OAT 2 | 0.241 ± 0.068 | 0.344 ± 0.070 | 0.293 ± 0.068 | Non-sign. |
OA 3 | 0.035 ± 0.007 | 0.044 ± 0.007 | 0.042 ± 0.007 | Non-sign. |
Item | Therapeutic | Environmental | Control | p-Value |
---|---|---|---|---|
NOR_ih 1 | 0.077 ± 0.022 | 0.061 ± 0.022 | 0.089 ± 0.022 | Non-sign. |
NOR_D1 2 | −0.015 ± 0.017 | −0.002 ± 0.017 | −0.004 ± 0.017 | Non-sign. |
NOR_DI 3 | −0.062 ± 0.274 | −0.057 ± 0.274 | 0.014 ± 0.274 | Non-sign. |
NOR_RI 4 | 0.469 ± 0.137 | 0.471 ± 0.137 | 0.507 ± 0.137 | Non-sign. |
NOR_VI 5 | 0.439 ± 0.086 | 0.432 ± 0.086 | 0.502 ± 0.086 | Non-sign. |
Variable | Measurement | Therapeutic | Environmental | Control | F-Value (df) |
---|---|---|---|---|---|
Contact with UNF 1 rat_1 | Total number | 11.22 ± 1.24 | 11.01 ± 1.24 | 12.14 ± 1.24 | 0.52 (2,35) |
Duration, s | 0.44 ± 0.04 | 0.44 ± 0.04 | 0.40 ± 0.04 | 0.41 (2,35) | |
Entry to the 1st Section | Total number | 3.85 ± 0.34 | 4.31 ± 0.34 | 4.08 ± 0.34 | 0.47 (2,35) |
Duration, s | 0.71± 0.05 | 0.70 ± 0.05 | 0.72 ± 0.05 | 0.07 (2,35) | |
Entry to the middle section | Total number | 4.00 ± 0.35 | 4.54 ± 0.35 | 4.46 ± 0.35 | 0.68 (2,35) |
Duration, s | 0.78 ± 0.07 | 0.89 ± 0.07 | 0.88 ± 0.07 | 0.76 (2,35) |
Variable | Measurement | Therapeutic | Environmental | Control | F-Value (df) |
---|---|---|---|---|---|
Repeated contact | Total number | 5.84 ± 0.75 | 5.85 ± 0.75 | 4.08 ± 0.75 | 1.88 (2,35) |
Duration, s | 0.23 ±0.06 | 0.22 ± 0.06 | 0.21 ± 0.06 | 0.05 (2,35) | |
Repeated entry to the 1st Section | Total number | 2.22 ± 0.38 | 2.92 ± 0.40 | 2.37 ± 0.38 | 1.24 (2,34) |
Duration, s | 0.45 ± 0.06 | 0.44 ± 0.06 | 0.34 ± 0.06 | 0.94 (2,34) | |
Repeated entry to the middle section | Total number | 4.60 ± 0.67 | 4.71 ± 0.67 | 5.06 ± 0.67 | 0.16 (2,35) |
Duration, s | 0.13 ± 0.03 | 0.20 ± 0.03 | 0.15 ± 0.03 | 1.79 (2,35) | |
Contact with UNF 1 rat_2 | Total number | 6.67 ± 0.92 | 7.20 ± 1.00 | 8.08 ± 0.88 | 0.63 (2,31) |
Duration, s | 0.17 ± 0.04 | 0.16 ± 0.04 | 0.24 ± 0.04 | 1.80 (2,31) | |
Entry to the 2nd Section | Total number | 2.33 ± 0.35 | 2.60 ± 0.38 | 2.54 ± 0.33 | 0.15 (2,31) |
Duration, s | 0.44 ± 0.05 | 0.40 ± 0.06 | 0.49 ± 0.05 | 0.77 (2,31) |
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Santariová, M.; Zadinová, K.; Vostrá-Vydrová, H.; Kolářová, M.F.; Kurhan, S.; Chaloupková, H. Effect of Environmental Concentration of Carbamazepine on the Behaviour and Gene Expression of Laboratory Rats. Animals 2023, 13, 2097. https://doi.org/10.3390/ani13132097
Santariová M, Zadinová K, Vostrá-Vydrová H, Kolářová MF, Kurhan S, Chaloupková H. Effect of Environmental Concentration of Carbamazepine on the Behaviour and Gene Expression of Laboratory Rats. Animals. 2023; 13(13):2097. https://doi.org/10.3390/ani13132097
Chicago/Turabian StyleSantariová, Milena, Kateřina Zadinová, Hana Vostrá-Vydrová, Martina Frühauf Kolářová, Sebnem Kurhan, and Helena Chaloupková. 2023. "Effect of Environmental Concentration of Carbamazepine on the Behaviour and Gene Expression of Laboratory Rats" Animals 13, no. 13: 2097. https://doi.org/10.3390/ani13132097