Heat Stress Alters the Effect of Eimeria maxima Infection on Ileal Amino Acids Digestibility and Transporters Expression in Meat-Type Chickens
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Design and Sampling
2.2. Apparent Digestibility
2.3. Nucleic Acid Extraction
2.4. Gene Expression
2.5. Statistical Analysis
3. Results
4. Discussion
4.1. The Effect of HS and E. maxima Infection on the Amino Acid AID
4.2. The Effect of HS and E. maxima Infection on the Apical Amino Acid Transporters
4.3. The Effect of HS and E. maxima Infection on the Basolateral Amino Acid Transporters
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Nutrient Transporter | Transporter Gene | Gene Bank Accession Number | Size | Align. | Primers Sequences |
---|---|---|---|---|---|
Apical amino acids | B0AT1 (SLC6A19) | XM_419056.8 | 117 | Forward | 5′CTGCCTGGGTTTGTCATCTAT3′ |
Reverse | 5′GCGCAGACGATACCTGTAAT3′ | ||||
b0,+AT (SLC7A9) | NM_001199133.1 | 113 | Forward | 5′GATCCCTGGAGCCTGAATTAC3′ | |
Reverse | 5′CTCCTTTCTGTTGTCCTGTTCT3′ | ||||
rBAT (SLC3A1) | XM_004935370.3 | 119 | Forward | 5′CTGAGAGCATCACAGCCTATTC3′ | |
Reverse | 5′GCCAGGTTCACTGCTGTATT3′ | ||||
Basolateral amino acids | TAT1 (SLC16A10) | NM_001321736.1 | 119 | Forward | 5′GCACCATCGAACCTCTGTATT3′ |
Reverse | 5′CACTAGACCAAGGCGTTTCTT3′ | ||||
LAT4 (SLC43A2) | XM_415803.6 | 113 | Forward | 5′GACTCGCAGCATCCCTAAAT3′ | |
Reverse | 5′GTGTCAGAGAAGTGGACGATATG3′ | ||||
CAT1 (SLC7A1) | NM_001145490.1 | 111 | Forward | 5′CGAACAACAGAGGAGACAGATAA3′ | |
Reverse | 5′GGGACACAGTATGGCTTTGA3′ | ||||
LAT1 (SLC7A5) | NM_001030579.2 | 98 | Forward | 5′GCCTTCTCCAATGACATCTTCT3′ | |
Reverse | 5′TAACGCAGCCACATCATACC3′ | ||||
SNAT1 (SLC38A1) | NM_001199603.1 | 108 | Forward | 5′CGCTAAATGCAACATCACCTATC3′ | |
Reverse | 5′TGGTGGGCAAAGCATACA3′ | ||||
SNAT2 (SLC38A2) | NM_001305439.1 | 127 | Forward | 5′GAACAAGTAGGGCCCTGTAATC3′ | |
Reverse | 5′GGGCAGAGCTTGATGTTATCT3′ | ||||
SNAT7 (SLC38A7) | XM_025154307.1 | 93 | Forward | 5′CAAGTTCACCATCAGCATCAC3′ | |
Reverse | 5′CTCAGAGAGCTGGCGTATTT3′ | ||||
B-actin | NM_205518.2 | 125 | Forward | 5′AGACATCAGGGTGTGATGGTTGGT3′ | |
Reverse | 5′TCCCAGTTGGTGACAATACCGTGT3′ |
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Ghareeb, A.F.A.; Schneiders, G.H.; Foutz, J.C.; Milfort, M.C.; Fuller, A.L.; Yuan, J.; Rekaya, R.; Aggrey, S.E. Heat Stress Alters the Effect of Eimeria maxima Infection on Ileal Amino Acids Digestibility and Transporters Expression in Meat-Type Chickens. Animals 2022, 12, 1554. https://doi.org/10.3390/ani12121554
Ghareeb AFA, Schneiders GH, Foutz JC, Milfort MC, Fuller AL, Yuan J, Rekaya R, Aggrey SE. Heat Stress Alters the Effect of Eimeria maxima Infection on Ileal Amino Acids Digestibility and Transporters Expression in Meat-Type Chickens. Animals. 2022; 12(12):1554. https://doi.org/10.3390/ani12121554
Chicago/Turabian StyleGhareeb, Ahmed F. A., Gustavo H. Schneiders, James C. Foutz, Marie C. Milfort, Alberta L. Fuller, Jianmin Yuan, Romdhane Rekaya, and Samuel E. Aggrey. 2022. "Heat Stress Alters the Effect of Eimeria maxima Infection on Ileal Amino Acids Digestibility and Transporters Expression in Meat-Type Chickens" Animals 12, no. 12: 1554. https://doi.org/10.3390/ani12121554