Silage Fermentation, Bacterial Community, and Aerobic Stability of Total Mixed Ration Containing Wet Corn Gluten Feed and Corn Stover Prepared with Different Additives
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. TMR Silage Making
2.2. Chemical Composition and Microbial Population Analysis
2.3. Microbial Diversity Analysis
2.4. Aerobic Stability Analysis
2.5. Statistical Analyses
3. Results
3.1. Characteristics of Fresh Materials and TMR before Ensiling
3.2. Chemical Compositions of the TMR Silages
3.3. Fermentation Quality of TMR Silages
3.4. Bacterial Community Analysis of Fresh TMR and TMR Silages
3.5. Aerobic Stability of TMR Silages
4. Discussion
4.1. Characteristics of TMR before Ensiling
4.2. Chemical Composition of TMR Silages
4.3. Fermentative Quality of TMR Silages
4.4. Bacterial Community Analysis of Fresh TMR and TMR Silages
4.5. Aerobic Stability of TMR Silages
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Items 1 | Corn Stover | WCGF | Corn Silage | TMR |
---|---|---|---|---|
DM, % of FW | 82.8 ± 0.32 | 61.4 ± 0.40 | 28.8 ± 0.31 | 44.6 ± 0.47 |
CP, % of DM | 9.68 ± 0.17 | 22.2 ± 0.21 | 6.92 ± 0.06 | 12.2 ± 0.31 |
aNDF, % of DM | 51.6 ± 0.67 | 36.5 ± 0.45 | 53.4 ± 1.90 | 48.5 ± 0.15 |
ADF, % of DM | 46.6 ± 0.83 | 9.56 ± 0.12 | 30.5 ± 0.60 | 24.9 ± 0.32 |
WSC, % of DM | 2.12 ± 0.09 | 4.26 ± 0.09 | 2.40 ± 0.26 | 5.53 ± 0.09 |
LAB (log cfu10/g FM) | 3.64 ± 0.09 | 2.66 ± 0.05 | 7.96 ± 0.07 | 4.74 ± 0.06 |
Yeast (log cfu10/g FM) | 6.74 ± 0.06 | 6.80 ± 0.08 | 2.97 ± 0.08 | 5.71 ± 0.07 |
Mold (log cfu10/g FM) | ND | ND | ND | 2.68 ± 0.09 |
Items 1 | Control | LAB | EN | LAB + EN |
---|---|---|---|---|
DM, % of FW | 42.1 ± 0.09 c | 43.4 ± 0.07 a | 42.6 ± 0.19 b | 43.5 ± 0.002 a |
CP, % of DM | 13.2 ± 0.04 | 13.6 ± 0.005 | 14.1 ± 0.42 | 13.4 ± 0.06 |
aNDF, % of DM | 43.5 ± 0.14 a | 42.5 ± 0.35 a | 40.6 ± 0.55 b | 39.6 ± 0.47 b |
ADF, % of DM | 22.8 ± 0.10 a | 22.1 ± 0.08 b | 21.4 ± 0.27 c | 20.3 ± 0.05 d |
WSC, % of DM | 2.06 ± 0.04 c | 2.59 ± 0.10 b | 2.78 ± 0.03 b | 3.82 ± 0.21 a |
Items 1 | Treatment 2 | Days of Ensiling | SEM 3 | p-Value 4 | |||||
---|---|---|---|---|---|---|---|---|---|
3 | 7 | 15 | 30 | T | D | T × D | |||
pH | control | 5.20 Aa | 4.87 Ba | 4.74 Ca | 4.29 Da | 0.02 | <0.0001 | <0.0001 | 0.0001 |
LAB | 4.89 Ab | 4.76 Bb | 4.39 Cc | 4.18 Dc | |||||
EN | 4.93 Ab | 4.79 Bab | 4.61Cb | 4.23 Db | |||||
LAB + EN | 4.85 Ab | 4.66 Bc | 4.35 Cc | 4.13 Dd | |||||
LA, % of DM | Control | 3.64 Dc | 3.86 Cc | 4.78 Bc | 6.83 Ac | 0.10 | <0.0001 | <0.0001 | <0.0001 |
LAB | 4.11 Dab | 4.94 Ca | 6.97 Ba | 8.53 Aa | |||||
EN | 3.87 Dbc | 4.55 Cb | 5.48 Bb | 7.99 Ab | |||||
LAB + EN | 4.29 Da | 5.06 Ca | 7.21 Ba | 8.74 Aa | |||||
AA, % of DM | Control | 0.67 Db | 0.92 Cb | 1.37 Bc | 1.65 Ab | 0.04 | <0.0001 | <0.0001 | <0.0001 |
LAB | 0.80 Da | 1.02 Ca | 2.11 Bb | 2.73 Aa | |||||
EN | 0.43 Dc | 0.86 Cb | 1.17 Bd | 1.33 Ac | |||||
LAB + EN | 0.85 Da | 1.11 Ca | 2.35 Ba | 2.89 Aa | |||||
AN, % of TN | Control | 1.98 Da | 2.27 Ca | 2.60 Ba | 2.77 Aa | 0.04 | <0.0001 | <0.0001 | 0.0003 |
LAB | 1.58 Bbc | 1.79 Ab | 1.89 Ac | 1.92 Ac | |||||
EN | 1.70 Cb | 2.05 Ba | 2.18 Bb | 2.46 Ab | |||||
LAB + EN | 14.3 Bc | 16.5 Ab | 17.1 Ad | 17.1 Ad | |||||
LAB (log cfu10/g FM) | Control | 8.70 Ac | 8.59 Bc | 8.30 Cc | 7.88 Dc | 0.20 | <0.0001 | <0.0001 | <0.0001 |
LAB | 8.95 Aa | 8.90 Aa | 8.72 Ba | 8.41 Ca | |||||
EN | 8.81 Ab | 8.75 Ab | 8.54 Bb | 8.25 Cb | |||||
LAB + EN | 8.93 Aa | 8.85 Ba | 8.71 Ca | 8.35 Dab | |||||
Yeast (logcfu10/g FM) | Control | 4.25 Aa | 3.27 Ba | 3.20 BCa | 3.15 Ca | 0.02 | <0.0001 | <0.0001 | <0.0001 |
LAB | 3.98 Ac | 3.00 Bc | 2.89 Cc | 2.70 Dc | |||||
EN | 4.18 Ab | 3.16 Bb | 3.15 Bb | 3.08 Cb | |||||
LAB + EN | 4.02 Ac | 3.03 Bc | 2.86 Cc | 2.71 Dc | |||||
Mold (logcfu10/g FM) | Control | 2.53 Aa | 2.40 Ba | 2.30 Ca | 2.15 Da | 0.02 | <0.0001 | <0.0001 | 0.66 |
LAB | 2.26 Ac | 2.15 Bc | 2.07 Cc | 1.92 Dc | |||||
EN | 2.42 Ab | 2.34 Bb | 2.23 Cb | 2.08 Db | |||||
LAB + EN | 2.23 Ac | 2.14 Bc | 2.08 Cc | 2.19 Dc |
Items 2 | Treatment 1 | SEM 3 | p-Value | ||||
---|---|---|---|---|---|---|---|
FM | control | LAB | EN | LAB + EN | |||
Reads | 79,928 | 79,935 | 80,021 | 80,002 | 80,004 | 2449.4 | 0.46 |
OTU | 385 a | 286 c | 353 ab | 313 bc | 261 c | 20.2 | 0.009 |
Chao1 | 432 | 361 | 409 | 345 | 340 | 30.2 | 0.20 |
Ace | 425 | 353 | 405 | 345 | 355 | 26.7 | 0.20 |
Coverage | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.0001 | 0.45 |
Shannon | 3.13 a | 3.17 a | 1.57 b | 2.58 a | 1.08 b | 0.233 | 0.0002 |
Simpson | 0.12 b | 0.1 b | 0.57 a | 0.2 b | 0.67 a | 0.06 | <0.0001 |
Items 1 | Treatment 2 | Days of Aerobic Exposure | SEM 3 | p-Value 4 | |||||
---|---|---|---|---|---|---|---|---|---|
0 | 3 | 6 | 9 | T | D | T × D | |||
pH | Control | 4.29 Da | 4.33 Cb | 4.59 Bb | 4.77 Ab | 0.009 | <0.0001 | <0.0001 | <0.0001 |
LAB | 4.18 Cc | 4.23 Bc | 4.24 Bc | 4.28 Ac | |||||
EN | 4.23 Db | 4.41 Ca | 4.69 Ba | 4.84 Aa | |||||
LAB + EN | 4.13 Bd | 4.12 Bd | 4.20 Ad | 4.21 Ad | |||||
LA, % of DM | Control | 6.83 Ac | 6.10 Bb | 5.55 Cc | 5.05 Dc | 0.13 | <0.0001 | <0.0001 | <0.0001 |
LAB | 8.53 Ca | 9.95 Aa | 9.60 Ab | 9.10 Bb | |||||
EN | 8.00 Ab | 5.75 Bb | 5.20 Cc | 4.45 Dd | |||||
LAB + EN | 8.74 Ca | 10.4 Ba | 11.4 Aa | 10.3 Ba | |||||
AA, % of DM | Control | 1.65 Ac | 1.55 Bc | 1.34 Cc | 1.18 Dc | 0.04 | <0.0001 | <0.0001 | 0.03 |
LAB | 3.39 ABb | 3.45 Ab | 3.23 BCb | 3.14 Cb | |||||
EN | 1.33 Ad | 1.17 Bd | 1.05 BCd | 0.99 Cd | |||||
LAB + EN | 3.83 Aa | 3.75 Aa | 3.52 Ba | 3.33 Ca | |||||
AN, % of TN | Control | 2.76 Da | 2.88 Ca | 3.31 Bb | 3.76 Ab | 0.05 | <0.0001 | <0.0001 | <0.0001 |
LAB | 1.92 Dc | 2.57 Cb | 3.04 Bbc | 3.36 Ac | |||||
EN | 2.46 Db | 2.95 Ca | 3.90 Ba | 4.33 Aa | |||||
LAB + EN | 1.71 Dd | 2.14 Cc | 2.81 Bc | 3.14 Ac | |||||
LAB (log10 cfu/g FM) | Control | 7.88 Ac | 7.65 Bc | 7.39 Cc | 7.25 Dc | 0.03 | <0.0001 | <0.0001 | <0.0001 |
LAB | 8.41 Aa | 8.26 Ba | 8.15 Ca | 8.04 Da | |||||
EN | 8.25 Ab | 8.11 Bb | 7.96 Cb | 7.90 Db | |||||
LAB + EN | 8.35 Aab | 8.23 Ba | 8.15 BCa | 8.08 Ca | |||||
Yeast (log10 cfu/g FM) | Control | 3.15 Da | 3.74 Cb | 5.69 Bb | 7.93 Ab | 0.02 | <0.0001 | <0.0001 | <0.0001 |
LAB | 2.70 Dc | 2.91 Cc | 3.51 Bc | 3.68 Ac | |||||
EN | 3.08 Db | 4.08 Ca | 6.44 Ba | 8.64 Aa | |||||
LAB + EN | 2.71 Bc | 2.71 Bd | 2.71 Bd | 2.75 Ac | |||||
Mold (log10 cfu/g FM) | Control | 2.15 Da | 2.80 Cb | 3.25 Bb | 4.39 Ab | 0.03 | <0.0001 | <0.0001 | <0.0001 |
LAB | 1.92 Bc | 1.97 Bc | 1.96 Bc | 2.04 Ac | |||||
EN | 2.08 Db | 2.94 Ca | 3.44 Ba | 4.79 Aa | |||||
LAB + EN | 1.92 Ca | 1.95 Bc | 1.96 Bc | 2.09 Ac |
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Zhang, G.; Fang, X.; Feng, G.; Li, Y.; Zhang, Y. Silage Fermentation, Bacterial Community, and Aerobic Stability of Total Mixed Ration Containing Wet Corn Gluten Feed and Corn Stover Prepared with Different Additives. Animals 2020, 10, 1775. https://doi.org/10.3390/ani10101775
Zhang G, Fang X, Feng G, Li Y, Zhang Y. Silage Fermentation, Bacterial Community, and Aerobic Stability of Total Mixed Ration Containing Wet Corn Gluten Feed and Corn Stover Prepared with Different Additives. Animals. 2020; 10(10):1775. https://doi.org/10.3390/ani10101775
Chicago/Turabian StyleZhang, Guangning, Xinpeng Fang, Guanzhi Feng, Yang Li, and Yonggen Zhang. 2020. "Silage Fermentation, Bacterial Community, and Aerobic Stability of Total Mixed Ration Containing Wet Corn Gluten Feed and Corn Stover Prepared with Different Additives" Animals 10, no. 10: 1775. https://doi.org/10.3390/ani10101775