Effects of Effective Microorganisms on Meat Quality, Microstructure of the Longissimus Lumborum Muscle, and Electrophoretic Protein Separation in Pigs Fed on Different Diets
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Maintenance and Feeding of Animals
2.2. Quality of Meat
2.3. Histochemical Analysis
2.4. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.4.1. Sample Preparation
2.4.2. Gel Electrophoresis
2.4.3. Image Analysis
2.5. Statistical Analysis
3. Results and Discussion
3.1. Quality of Meat
3.2. Microstructure of the Longissimus Lumborum Muscle
3.3. Percentage of Selected Muscle Fiber Proteins in Longissimus Lumborum Muscle
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
Ethics Approval
References
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Dietary Value | Control | E1 | E2 | |||
---|---|---|---|---|---|---|
Phase I (30–70 kg) | Phase II (70–115 kg) | Phase I (30–70 kg) | Phase II (70–115 kg) | Phase I (30–70 kg) | Phase II (70–115 kg) | |
Dry matter, g | 877 | 875 | 877 | 875 | 877 | 875 |
Metabolic energy, MJ | 13.39 | 13.11 | 13.32 | 13.09 | 13.32 | 13.12 |
Total protein, g | 170 | 159 | 171 | 157 | 171 | 160 |
Fat, g | 27 | 20 | 29 | 20 | 29 | 20 |
Lysine, g | 10.6 | 9.7 | 10.6 | 9.7 | 10.6 | 9.7 |
Calcium, g | 5.9 | 5.8 | 5.9 | 5.8 | 5.9 | 5.8 |
Phosphorus, g | 5.3 | 5.2 | 5.3 | 5.2 | 5.3 | 5.2 |
Sodium, g | 1.7 | 1.7 | 1.7 | 1.7 | 1.7 | 1.7 |
Vitamin A, IU | 10.000 | 10.000 | 10.000 | 10.000 | 10.000 | 10.000 |
Vitamin D, IU | 2.200 | 2.200 | 2.200 | 2.200 | 2.200 | 2.200 |
Vitamin E, IU | 80 | 80 | 80 | 80 | 80 | 80 |
Composition of the fodder, %: | ||||||
Soy meal 46% BO | 16 | 12 | 10 | 4 | 10 | - |
Wheat 12% | 20 | 20 | 20 | 20 | 20 | 20 |
Barley 12% | 35 | 45 | 30 | 41 | 30 | 35 |
Triticale 10% | 25.3 | 20 | 26 | 20 | 26 | 20 |
Soybean oil | 1 | 0.3 | 1.3 | 0.3 | 1.3 | 0.3 |
Lupine 37% | - | - | 7 | 9 | 7 | 12 |
Pea 21% | - | - | 3 | 3 | 3 | 10 |
PORKOVITAL T PEA 2.5% | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
SELACID GG DRY 25 BR | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 |
Total | 100 | 100 | 100 | 100 | 100 | 100 |
Control Group (C) | Experimental Group (E1) | Experimental Group (E2) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
C | C + EM | E1 | E1 + EM | E2 | E2 + EM | ||||||
n = 25 | n = 25 | n = 25 | n = 25 | n = 25 | n = 25 | ||||||
♂ 13♀ 12 | ♂ 12 ♀ 13 | ♂ 12 ♀ 13 | ♂ 13 ♀ 12 | ♂ 13 ♀ 12 | ♂ 12 ♀ 13 | ||||||
Body Weight at Slaughter, kg | |||||||||||
♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ |
111.27 ± 5.70 | 107.70 ± 8.45 | 113.42 ± 4.94 | 112.04 ± 3.07 | 115.63 ± 4.67 | 109.00 ± 7.29 | 115.86 ± 4.43 | 112.50 ± 4.21 | 113.82 ± 4.88 | 111.55 ± 4.57 | 115.86 ± 4.64 | 115.56 ± 4.30 |
Traits | C | E1 | E2 | |||
---|---|---|---|---|---|---|
C | C + EM | E1 | E1 + EM | E2 | E2 + EM | |
pH48 | 5.14 ± 0.12 | 5.09 ± 0.09 | 5.13 ± 0.06 | 5.19 ± 0.10 | 5.11 ± 0.05 | 5.13 ± 0.07 |
WHC, loose water % | 15.57 a ± 2.49 p = 0.016 | 18.16 b ± 2.50 p = 0.016 | 17.77 ± 2.13 | 17.01 ± 1.70 | 18.89 ± 3.72 | 16.28 ± 1.88 |
Thermal leakage, % | 24.39 ± 1.50 | 24.25 ± 2.54 | 24.44 ± 0.63 | 24.80 * ± 1.96 | 24.80 a ± 2.50 p = 0.024 | 22.38 b* ± 1.25 p = 0.024 |
L*—Lightness | 56.30 ± 1.92 | 57.59 ± 3.48 | 56.49 ± 2.36 | 55.65 ± 2.55 | 56.13 ± 1.15 | 56.39 ± 2.52 |
a*—Redness | 13.91 ± 0.66 | 14.13 ± 0.86 | 13.99 ± 1.04 | 14.41 ± 0.56 | 14.21 ± 0.78 | 14.00 ± 0.98 |
b *—Yelowness | 4.50 ± 0.64 | 4.25 ± 0.77 | 4.27 ± 0.96 | 4.51 ± 0.80 | 4.89 ± 0.55 | 4.56 ± 0.91 |
Marbling, score | 1.87 ± 0.79 | 1.47 ± 0.50 | 1.59 ± 0.49 | 1.70 ± 0.60 | 1.39 ± 0.48 | 1.55 ± 0.51 |
Tenderness, N | 47.94 ± 2.80 | 46.83 * ± 5.74 | 47.27 a ± 7.62 p = 0.030 | 56.27 b* ± 7.28 p = 0.030 | 53.57 ± 5.78 | 51.34 * ± 5.03 |
Loin eye area, cm2 | 53.10 ± 5.67 | 55.76 ± 6.15 | 53.41 ± 4.45 | 52.62 * ± 2.21 | 57.59 ± 4.96 | 57.93 * ± 3.31 |
Traits | C | E1 | E2 | ||||
---|---|---|---|---|---|---|---|
C | C + EM | E1 | E1 + EM | E2 | E2 + EM | ||
Percentage of muscle fibers, % | STO | 13.60 ± 2.50 | 16.33 ± 6.60 | 14.36 ± 4.68 | 14.51 ± 3.60 | 16.10 ± 4.41 | 15.41 ± 4.77 |
FTO | 13.69 ± 2.82 | 13.52 ± 3.57 | 13.80 ± 2.40 | 14.05 ± 3.27 | 14.70 ± 3.76 | 15.13 ± 2.20 | |
FTG | 72.71 ± 3.21 | 70.15 ± 9.25 | 71.84 ± 6.35 | 71.44 ± 3.49 | 69.20 ± 16.14 | 69.46 ± 20.62 | |
Fiber diameter, µm | STO | 35.03 ± 4.34 | 35.86 ± 4.69 | 38.86 ± 5.61 | 40.82 ± 5.21 | 37.11 ± 3.38 | 39.53 ± 2.65 |
FTO | 35.44 ± 6.52 | 40.66 ± 6.63 | 35.78 a ± 3.88 p = 0.003 | 43.17 b ± 3.51 p = 0.003 | 37.24 a ± 5.46 p = 0.028 | 39.42 b ± 2.26 p = 0.028 | |
FTG | 47.14 ± 4.50 | 52.30 ± 8.71 | 44.68 a* ± 5.16 p = 0.012 | 50.57 b ± 3.46 p = 0.012 | 51.26 * ± 2.63 | 52.27 ± 3.26 | |
Muscle fiber density (fiber number/1,5 mm2) | Sum | 244 ± 48 | 228 ± 67 | 243 ± 60 | 205 ± 22 | 222 ± 20 | 232 ± 23 |
STO | 35 ± 10 | 34 ± 9 | 33 ± 7 | 30 ± 8 | 34 ± 10 | 28 ± 10 | |
FTO | 34 ± 10 | 31 ± 11 | 33 ± 7 | 29 ± 7 | 27 ± 8 | 31 ± 7 | |
FTG | 175 ± 34 | 163 ± 65 | 177 ± 52 | 146 ± 16 | 161 ± 13 | 173 ± 21 | |
Total number of muscle fibers (×1000) (TNF) | 744 a ± 368 p = 0.044 | 694 b ± 488 p = 0.044 | 743 ± 371 | 617 ± 254 | 660 ± 406 | 652 ± 439 | |
Intramuscular fat content, (histochemically) % | 1.66 ± 1.55 | 2.27 ± 1.13 | 1.84 ± 1.21 | 1.67 ± 0.96 | 1.66 ± 2.05 | 2.45 ± 1.79 | |
Normal fibers, % | 97.22 ± 2.02 | 97.36 ± 1.97 | 95.95 ± 2.72 | 98.27 ± 1.02 | 96.72 ± 1.71 | 96.60 ± 1.86 | |
Giant fibers, % | 1.99 ± 1.84 | 1.29 ± 1.29 | 1.76 ± 1.80 | 1.10 ± 1.17 | 1.87 ± 1.16 | 1.95 ± 1.50 | |
Fiber necrosis, % | 0.11 ± 0.18 | 0.34 ± 0.53 | 0.84 ± 3.03 | 0.80 ± 0.77 | 0.60 ± 0.82 | 0.30 ± 0.46 | |
Fiber splitting, % | 0.68 a* ± 0.25 p = 0.041 | 1.01 b ± 0.92 p = 0.041 | 1.45 a* ± 1.1 p = 0.024 | 0.83 b ± 0.81 p = 0.024 | 0.81 a* ± 0.77 p = 0.040 | 1.15 b ± 0.82 p = 0.040 |
Proteins Molecular Weight (kDa) | C | E1 | E2 | |||
---|---|---|---|---|---|---|
C | C + EM | E1 | E1 + EM | E2 | E2 + EM | |
3000–3700 titin | 7.407 *± 0.428 | 7.385 * ± 0.431 | 6.152 * ± 0.836 | 5.785 * ± 1.051 | 6.708 ± 1.173 | 6.832 ± 0.760 |
205 myosin and titin degradation products | 18.118 a ± 0.385 p = 0.027 | 17.102 b ± 0.883 p = 0.027 | 17.118 ± 1.171 | 15.807 ± 1.789 | 18.060 ± 0.892 | 17.912 ± 2.195 |
105 alpha actinin | 5.402 ± 1.75 | 6.022 ± 0.406 | 5.732 ± 1.654 | 5.365 ± 1.496 | 6.627 ± 0.624 | 6.382 ± 1.017 |
100–43(kDa) | 6.070 ± 0.492 | 6.305 ± 0.896 | 6.112 ± 0.552 | 7.047 ± 0.905 | 6.588 ± 0.796 | 6.920 ± 0.578 |
42 actin | 32.207 ± 3.048 | 31.270 ± 1.596 | 33.010 ± 2.380 | 33.885 * ± 3.045 | 30.063 ± 2.294 | 30.002 * ± 2.476 |
36 troponin-T | 10.057 ± 0.790 | 10.783 ± 0.646 | 10.568 b ± 0.389 p = 0.015 | 11.172 a ± 1.296 p = 0.015 | 9.700 ± 1.196 | 9.918 ± 1.142 |
35- 18 including troponin-T degradation products | 9.092 ± 0.877 | 9.287 ± 0.776 | 9.563 ± 0.727 | 9.525 ± 1.599 | 9.492 ± 2.204 | 9.392 ± 1.215 |
17 myoglobin | 5.478 ± 2.378 | 5.282 ± 0.823 | 5.365 ± 0.936 | 5.4383 ± 1.189 | 6.110 ± 0.834 | 6.347 ± 1.513 |
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Reszka, P.; Cygan-Szczegielniak, D.; Jankowiak, H.; Cebulska, A.; Mikołajczak, B.; Bogucka, J. Effects of Effective Microorganisms on Meat Quality, Microstructure of the Longissimus Lumborum Muscle, and Electrophoretic Protein Separation in Pigs Fed on Different Diets. Animals 2020, 10, 1755. https://doi.org/10.3390/ani10101755
Reszka P, Cygan-Szczegielniak D, Jankowiak H, Cebulska A, Mikołajczak B, Bogucka J. Effects of Effective Microorganisms on Meat Quality, Microstructure of the Longissimus Lumborum Muscle, and Electrophoretic Protein Separation in Pigs Fed on Different Diets. Animals. 2020; 10(10):1755. https://doi.org/10.3390/ani10101755
Chicago/Turabian StyleReszka, Patrycja, Dorota Cygan-Szczegielniak, Hanna Jankowiak, Aleksandra Cebulska, Beata Mikołajczak, and Joanna Bogucka. 2020. "Effects of Effective Microorganisms on Meat Quality, Microstructure of the Longissimus Lumborum Muscle, and Electrophoretic Protein Separation in Pigs Fed on Different Diets" Animals 10, no. 10: 1755. https://doi.org/10.3390/ani10101755