Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Acanthamoeba Strain
2.2. Stock Suspension of Acanthamoeba Cysts
2.3. Stock Suspension of Escherichia coli
2.4. Contact Lenses
2.5. Plasma Generation
2.6. Experimental Design
2.7. Exposure of Acanthamoeba Cysts
2.7.1. Cysts in Suspension
2.7.2. Cyst-Contaminated Contact Lenses
2.8. Evaluation of Cysts Viability
2.8.1. Cysts in Suspension
2.8.2. Cyst-Contaminated Contact Lenses
2.9. Physical Parameters of Contact Lenses
2.10. Infrared and Raman Spectroscopy
3. Results
3.1. Inactivation of Cysts
3.2. Physical Parameters of Lenses
3.3. Spectral Properties
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Khan, N.A. Acanthamoeba: Biology and increasing importance in human health. FEMS Microbiol. Rev. 2006, 30, 564–595. [Google Scholar] [CrossRef] [Green Version]
- Mazur, T.; Hadaś, E.; Iwanicka, I. The duration of the cyst stage and the viability and virulence of Acanthamoeba isolates. Trop. Med. Parasitol. 1995, 46, 106–108. [Google Scholar] [PubMed]
- Sriram, R.; Shoff, M.; Booton, G.; Fuerst, P.; Visvesvara, G.S. Survival of Acanthamoeba cysts after desiccation for more than 20 years. J. Clin. Microbiol. 2008, 46, 4045–4048. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Coulon, C.; Collignon, A.; McDonnell, G.; Thomas, V. Resistance of Acanthamoeba cysts to disinfection treatments used in health care settings. J. Clin. Microbiol. 2010, 48, 2689–2697. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Niederkorn, J.Y. The biology of Acanthamoeba keratitis. Exp. Eye Res. 2021, 202, 108365. [Google Scholar] [CrossRef] [PubMed]
- Marciano-Cabral, F.; Cabral, G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev. 2003, 16, 273–307. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Lorenzo-Morales, J.; Khan, N.A.; Walochnik, J. An update on Acanthamoeba keratitis: Diagnosis, pathogenesis and treatment. Parasite 2015, 22, 10. [Google Scholar] [CrossRef] [Green Version]
- Fears, A.C.; Metzinger, R.C.; Killeen, S.Z.; Reimers, R.S.; Roy, C.J. Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii. J. Ophthalmic. Inflamm. Infect. 2018, 8, 19. [Google Scholar] [CrossRef] [PubMed]
- Kobayashi, T.; Gibbon, L.; Mito, T.; Shiraishi, A.; Uno, T.; Ohashi, Y. Efficacy of commercial soft contact lens disinfectant solutions against Acanthamoeba. Jpn. J. Ophthalmol. 2011, 55, 547–557. [Google Scholar] [CrossRef]
- Lakhundi, S.; Khan, N.A.; Siddiqui, R. Inefficacy of marketed contact lens disinfection solutions against keratitis-causing Acanthamoeba castellanii belonging to the T4 genotype. Exp. Parasitol. 2014, 141, 122–128. [Google Scholar] [CrossRef]
- Gabriel, M.M.; McAnally, C.; Bartell, J.; Walters, R.; Clark, L.; Crary, M.; Shannon, S. Biocidal Efficacy of a Hydrogen Peroxide Lens Care Solution Incorporating a Novel Wetting Agent. Eye Contact Lens. 2019, 45, 164–170. [Google Scholar] [CrossRef] [PubMed]
- Padzik, M.; Hendiger, E.B.; Żochowska, A.; Szczepaniak, J.; Baltaza, W.; Pietruczuk-Padzik, A.; Olędzka, G.; Chomicz, L. Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains. Ann. Agric. Environ. Med. 2019, 26, 198–202. [Google Scholar] [CrossRef]
- Hiti, K.; Walochnik, J.; Faschinger, C.; Haller-Schober, E.M.; Aspöck, H. Microwave treatment of contact lens cases contaminated with Acanthamoeba. Cornea 2001, 20, 467–470. [Google Scholar] [CrossRef]
- Heaselgrave, W.; Patel, N.; Kilvington, S.; Kehoe, S.C.; McGuigan, K.G. Solar disinfection of poliovirus and Acanthamoeba polyphaga cysts in water—A laboratory study using simulated sunlight. Lett. Appl. Microbiol. 2006, 43, 125–130. [Google Scholar] [CrossRef] [PubMed]
- Heaselgrave, W.; Shama, G.; Andrew, P.W.; Kong, M.G. Inactivation of Acanthamoeba spp. and Other Ocular Pathogens by Application of Cold Atmospheric Gas Plasma. Appl. Environ. Microbiol. 2016, 82, 3143–3148. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ehlbeck, J.; Schnabel, U.; Polak, M.; Winter, J.; von Woedtke, T.; Brandenburg, R.; von dem Hagen, T.; Weltmann, K.D. Low temperature atmospheric pressure plasma sources for microbial decontamination. J. Phys. D Appl. Phys. 2011, 44, 013002. [Google Scholar] [CrossRef] [Green Version]
- Khun, J.; Scholtz, V.; Hozák, P.; Fitl, P.E.; Julák, J. Various DC-driven point-to-plain discharges as non-thermal plasma sources and their bactericidal effects. Plasma Sources Sci. Technol. 2018, 27, 065002. [Google Scholar] [CrossRef]
- Laroussi, M. Low-Temperature Plasmas for Medicine? IEEE Trans. Plasma Sci. 2009, 37, 714–726. [Google Scholar] [CrossRef]
- Laroussi, M.; Akan, T. Arc-Free Atmospheric Pressure Cold Plasma Jets: A Review. Plasma Process. Polym. 2007, 4, 777–788. [Google Scholar] [CrossRef]
- Laroussi, M.; Lu, X.; Keidar, M. Perspective: The physics, diagnostics, and applications of atmospheric pressure low temperature plasma sources used in plasma medicine. J. Appl. Phys. 2017, 122, 020901. [Google Scholar] [CrossRef]
- Šimončicová, J.; Kryštofová, S.; Medvecká, V.; Ďurišová, K.; Kaliňáková, B. Technical applications of plasma treatments: Current state and perspectives. Appl. Microbiol. Biotechnol. 2019, 103, 5117–5129. [Google Scholar] [CrossRef] [PubMed]
- Yousfi, M.; Merbahi, N.; Sarrette, J.-P.; Eichwald, O.; Ricard, A.; Gardou, J.-P.; Ducasse, O.; Benhenni, M. Non Thermal Plasma Sources of Production of Active Species for Biomedical Uses: Analyses, Optimization and Prospect. In Biomedical Engineering—Frontiers and Challenges; Fazel-Rezai, R., Ed.; IntechOpen: London, UK, 2011; pp. 99–124. [Google Scholar]
- Bourke, P.; Ziuzina, D.; Han, L.; Cullen, P.J.; Gilmore, B.F. Microbiological interactions with cold plasma. J. Appl. Microbiol. 2017, 123, 308–324. [Google Scholar] [CrossRef] [Green Version]
- Julák, J.; Scholtz, V. The potential for use of non-thermal plasma in microbiology and medicine. Epidemiol. Mikrobiol. Imunol. 2020, 69, 29–37. [Google Scholar] [PubMed]
- Tendero, C.; Tixier, C.; Tristant, P.; Desmaison, J.; Leprince, P. Atmospheric pressure plasmas: A review. Spectrochim. Acta Part B At. Spectrosc. 2006, 61, 2–30. [Google Scholar] [CrossRef]
- Weltmann, K.D.; von Woedtke, T. Plasma medicine—Current state of research and medical application. Plasma Phys. Control. Fusion 2016, 59, 014031. [Google Scholar] [CrossRef]
- Woedtke, T.V.; Emmert, S.; Metelmann, H.-R.; Rupf, S.; Weltmann, K.-D. Perspectives on cold atmospheric plasma (CAP) applications in medicine. Phys. Plasmas 2020, 27, 070601. [Google Scholar] [CrossRef]
- Metelmann, H.-R.; von Woedtke, T.; Weltmann, K.-D. (Eds.) Comprehensive Clinical Plasma Medicine: Cold Physical Plasma for Medical Application; Springer International Publishing: Cham, Switzerland, 2018; p. 526. [Google Scholar]
- Gherardi, M.; Tonini, R.; Colombo, V. Plasma in Dentistry: Brief History and Current Status. Trends Biotechnol. 2017, 36, 583–585. [Google Scholar] [CrossRef] [PubMed]
- Gweon, B.; Kim, K.; Choe, W.; Shin, J.H. Therapeutic Uses of Atmospheric Pressure Plasma: Cancer and Wound. In Biomedical Engineering: Frontier Research and Converging Technologies; Jo, H., Jun, H.-W., Shin, J., Lee, S., Eds.; Springer International Publishing: Cham, Switzerland, 2016; pp. 357–385. [Google Scholar]
- Keidar, M.; Yan, D.; Beilis, I.I.; Trink, B.; Sherman, J.H. Plasmas for Treating Cancer: Opportunities for Adaptive and Self-Adaptive Approaches. Trends Biotechnol. 2018, 36, 586–593. [Google Scholar] [CrossRef]
- Graves, D.B. The emerging role of reactive oxygen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology. J. Phys. D Appl. Phys. 2012, 45, 263001. [Google Scholar] [CrossRef]
- Kelly, S.; Turner, M. Atomic oxygen patterning from a biomedical needle-plasma source. J. Appl. Phys. 2013, 114, 123301. [Google Scholar] [CrossRef] [Green Version]
- Sysolyatina, E.; Mukhachev, A.; Yurova, M.; Grushin, M.; Karalnik, V.; Petryakov, A.; Trushkin, N.; Ermolaeva, S.; Akishev, Y. Role of the Charged Particles in Bacteria Inactivation by Plasma of a Positive and Negative Corona in Ambient Air. Plasma Process. Polym. 2014, 11, 315–334. [Google Scholar] [CrossRef]
- Liu, D.X.; Liu, Z.C.; Chen, C.; Yang, A.J.; Li, D.; Rong, M.Z.; Chen, H.L.; Kong, M.G. Aqueous reactive species induced by a surface air discharge: Heterogeneous mass transfer and liquid chemistry pathways. Sci. Rep. 2016, 6, 23737. [Google Scholar] [CrossRef]
- Al-sharify, Z.T.; Alsharify, T.A.; Al-Obaidy, B.; Al-Azawi, A. Investigative Study on the Interaction and Applications of Plasma Activated Water(PAW). IOP Conf. Ser. Mater. Sci. Eng. 2020, 870, 012042. [Google Scholar] [CrossRef]
- Julák, J.; Hujacová, A.; Scholtz, V.; Khun, J.; Holada, K. Contribution to the Chemistry of Plasma-Activated Water. Plasma Phys. Rep. 2018, 44, 125–136. [Google Scholar] [CrossRef]
- Zhou, R.; Zhou, R.; Wang, P.; Xian, Y.; Mai-Prochnow, A.; Lu, X.; Cullen, P.J.; Ostrikov, K.; Bazaka, K. Plasma-activated water: Generation, origin of reactive species and biological applications. J. Phys. D Appl. Phys. 2020, 53, 303001. [Google Scholar] [CrossRef]
- Terrier, O.; Essere, B.; Yver, M.; Barthélémy, M.; Bouscambert-Duchamp, M.; Kurtz, P.; VanMechelen, D.; Morfin, F.; Billaud, G.; Ferraris, O.; et al. Cold oxygen plasma technology efficiency against different airborne respiratory viruses. J. Clin. Virol. 2009, 45, 119–124. [Google Scholar] [CrossRef] [PubMed]
- Xia, T.; Kleinheksel, A.; Lee, E.M.; Qiao, Z.; Wigginton, K.R.; Clack, H.L. Inactivation of airborne viruses using a packed bed non-thermal plasma reactor. J. Phys. D Appl. Phys. 2019, 52, 255201. [Google Scholar] [CrossRef]
- Aman Mohammadi, M.; Ahangari, H.; Zabihzadeh Khajavi, M.; Yousefi, M.; Scholtz, V.; Hosseini, S.M. Inactivation of viruses using nonthermal plasma in viral suspensions and foodstuff: A short review of recent studies. J. Food Saf. 2021, e12919. [Google Scholar] [CrossRef]
- Julak, J.; Scholtz, V.; Vaňková, E. Medically important biofilms and non-thermal plasma. World J. Microbiol. Biotechnol. 2018, 34, 1–15. [Google Scholar] [CrossRef]
- Misra, N.N.; Yadav, B.; Roopesh, M.S.; Jo, C. Cold Plasma for Effective Fungal and Mycotoxin Control in Foods: Mechanisms, Inactivation Effects, and Applications. Compr. Rev. Food Sci. Food Saf. 2019, 18, 106–120. [Google Scholar] [CrossRef] [Green Version]
- Julák, J.; Soušková, H.; Scholtz, V.; Kvasničková, E.; Savická, D.; Kříha, V. Comparison of fungicidal properties of non-thermal plasma produced by corona discharge and dielectric barrier discharge. Folia Microbiol. 2018, 63, 63–68. [Google Scholar] [CrossRef]
- Paldrychová, M.; Vaňková, E.; Scholtz, V.; Julák, J.; Sembolová, E.; Matátková, O.; Masák, J. Effect of non-thermal plasma on AHL-dependent QS systems and biofilm formation in Pseudomonas aeruginosa: Difference between non-hospital and clinical isolates. AIP Adv. 2019, 9, 055117. [Google Scholar] [CrossRef] [Green Version]
- Hayes, J.; Kirf, D.; Garvey, M.; Rowan, N. Disinfection and toxicological assessments of pulsed UV and pulsed-plasma gas-discharge treated-water containing the waterborne protozoan enteroparasite Cryptosporidium parvum. J. Microbiol. Methods 2013, 94, 325–337. [Google Scholar] [CrossRef] [PubMed]
- Rowan, N. Defining Established and Emerging Microbial Risks in the Aquatic Environment: Current Knowledge, Implications, and Outlooks. Int. J. Microbiol. 2011, 2011, 462832. [Google Scholar] [CrossRef] [Green Version]
- Wang, X.-Q.; Wang, F.-P.; Chen, W.; Huang, J.; Bazaka, K.; Ostrikov, K. Non-equilibrium plasma prevention of Schistosoma japonicum transmission. Sci. Rep. 2016, 6, 35353. [Google Scholar] [CrossRef] [Green Version]
- Hejzlarová, S.; Chanová, M.; Khun, J.; Julák, J.; Scholtz, V. Inactivation of Schistosoma Using Low-Temperature Plasma. Microorganisms 2021, 9, 32. [Google Scholar] [CrossRef] [PubMed]
- Cerva, L. Some further characteristics of the growth of Naegleria fowleri and N. gruberi in axenic culture. Folia Parasitol. 1978, 25, 1–8. [Google Scholar]
- Booton, G.C.; Kelly, D.J.; Chu, Y.W.; Seal, D.V.; Houang, E.; Lam, D.S.; Byers, T.J.; Fuerst, P.A. 18S ribosomal DNA typing and tracking of Acanthamoeba species isolates from corneal scrape specimens, contact lenses, lens cases, and home water supplies of Acanthamoeba keratitis patients in Hong Kong. J. Clin. Microbiol. 2002, 40, 1621–1625. [Google Scholar] [CrossRef] [Green Version]
- De Jonckheere, J.F. Growth characteristics, cytopathic effect in cell culture, and virulence in mice of 36 type strains belonging to 19 different Acanthamoeba spp. Appl. Environ. Microbiol. 1980, 39, 681–685. [Google Scholar] [CrossRef] [Green Version]
- Johnston, S.P.; Sriram, R.; Qvarnstrom, Y.; Roy, S.; Verani, J.; Yoder, J.; Lorick, S.; Roberts, J.; Beach, M.J.; Visvesvara, G. Resistance of Acanthamoeba cysts to disinfection in multiple contact lens solutions. J. Clin. Microbiol. 2009, 47, 2040–2045. [Google Scholar] [CrossRef] [Green Version]
- Scholtz, V.; Julák, J.; Kříha, V. The Microbicidal Effect of Low-Temperature Plasma Generated by Corona Discharge: Comparison of Various Microorganisms on an Agar Surface or in Aqueous Suspension. Plasma Process. Polym. 2010, 7, 237–243. [Google Scholar] [CrossRef]
- Soušková, H.; Scholtz, V.; Julák, J.; Kommová, L.; Savická, D.; Pazlarová, J. The survival of micromycetes and yeasts under the low-temperature plasma generated in electrical discharge. Folia Microbiol. 2011, 56, 77–79. [Google Scholar] [CrossRef] [PubMed]
- Julák, J.; Scholtz, V.; Kotúčová, S.; Janoušková, O. The persistent microbicidal effect in water exposed to the corona discharge. Phys. Med. 2012, 28, 230–239. [Google Scholar] [CrossRef] [PubMed]
- Scholtz, V.; Kommová, L.; Julák, J. The Influence of Parameters of Stabilized Corona Discharge on its Microbicidal Effect. Acta Phys. Pol. A 2011, 119, 803–806. [Google Scholar] [CrossRef]
- Hughes, R.; Kilvington, S. Comparison of hydrogen peroxide contact lens disinfection systems and solutions against Acanthamoeba polyphaga. Antimicrob. Agents Chemother. 2001, 45, 2038–2043. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hughes, R.; Andrew, P.W.; Kilvington, S. Enhanced killing of Acanthamoeba cysts with a plant peroxidase-hydrogen peroxide-halide antimicrobial system. Appl. Environ. Microbiol. 2003, 69, 2563–2567. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kilvington, S.; Winterton, L. Fibrous Catalyst-Enhanced Acanthamoeba Disinfection by Hydrogen Peroxide. Opt. Vis. Sci. 2017, 94, 1022–1028. [Google Scholar] [CrossRef] [PubMed]
Exposure (Min) | Positive Discharge | Negative Discharge |
---|---|---|
20 | 0 | 10 |
25 | 0 | 20 |
30 | 20 | 60 |
35 | 10 | 80 |
40 | 60 | 100 |
45 | 80 | 100 |
50 | 80 | 100 |
55 | 60 | 100 |
Exposure (Min) | Soflens | Biofinity |
---|---|---|
35 | 60 | 20 |
40 | 80 | 40 |
45 | 80 | 80 |
50 | 100 | 100 |
55 | 100 | 100 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Měřínská, T.; Scholtz, V.; Khun, J.; Julák, J.; Nohýnková, E. Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma. Microorganisms 2021, 9, 1879. https://doi.org/10.3390/microorganisms9091879
Měřínská T, Scholtz V, Khun J, Julák J, Nohýnková E. Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma. Microorganisms. 2021; 9(9):1879. https://doi.org/10.3390/microorganisms9091879
Chicago/Turabian StyleMěřínská, Tereza, Vladimír Scholtz, Josef Khun, Jaroslav Julák, and Eva Nohýnková. 2021. "Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma" Microorganisms 9, no. 9: 1879. https://doi.org/10.3390/microorganisms9091879