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Communication
Peer-Review Record

Decontamination Strategies Used for AFB Culture Significantly Reduce the Viability of Mycobacterium abscessus Complex in Sputum Samples from Patients with Cystic Fibrosis

Microorganisms 2021, 9(8), 1597; https://doi.org/10.3390/microorganisms9081597
by Dominic Stephenson 1,2, Audrey Perry 1, Andrew Nelson 2, Ali E. Robb 1, Matthew F. Thomas 3, Stephen J. Bourke 4, John D. Perry 1,2,* and Amanda L. Jones 2
Reviewer 2: Anonymous
Microorganisms 2021, 9(8), 1597; https://doi.org/10.3390/microorganisms9081597
Submission received: 30 June 2021 / Revised: 19 July 2021 / Accepted: 23 July 2021 / Published: 27 July 2021
(This article belongs to the Special Issue Mycobacterial Infections: Diagnostics, Biomarkers and Treatment)

Round 1

Reviewer 1 Report

This is a highly significant and well designed contribution to the operation of clinical microbiology laboratories.

The following points should be addressed.

The reductions (line 131 etc) being not statistically significant is puzzling. Please provide an explanation.

Please provide a reference for the MALDI-TOF method for identifying the colony types.

Please separate 0.5 or 1 from "N". Also, 0.5 N is preferable to N/2

RGM medium does not appear to be available commercially. Please provide its composition.

In Table 1, please reduce the space between "decontaminant" and "ratio", change the font of the title of the last column, and use 1:1 instead of 1 : 1.

Author Response

Please see the attached file entitled "Response to reviewers"

Author Response File: Author Response.docx

Reviewer 2 Report

I found the article an interesting reading. I just have minor comments:

  • RGM medium: I think the readers would benefit from having more information about this medium. For instance, the abbreviation stands for, it is based on what, what makes it selective, etc.
  • Figure 1: Error bars should be added to the graph.

 

Author Response

Please see the attached file entitled "Response to reviewers".

Author Response File: Author Response.docx

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