Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site Description and Soil Sampling
2.2. Aerobic CH4 Oxidation Experiment and DNA-SIP Gradient Fractionation
2.3. Analyses of Active Methanotrophic Communities
2.4. Statistical Analyses
3. Results
4. Discussion
4.1. Composition and Adaptation of Methanotrophs in Field Mangrove Soils
4.2. Active Methanotrophs Identified with 13CH4 DNA-SIP
4.3. pmoA and 16S rRNA Sequences
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Site | Season | Surface Water Salinity (psu) | pH | Soil Redox (mV) | Soil Texture | TOC | TN | SbOC | NH4+ | NO3− | SbON | TDN | PMN |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(%) | (mg C kg−1 Soil) | (mg N kg−1 Soil) | |||||||||||
Upstream (Guandu) | Winter | 20 | 3.6 ± 0.0 | −208 ± 73 | silty clay | 4.0 ± 0.8 | 0.3 ± 0.0 | 21.4 ± 1.8 | 10.0 ± 1.2 | 0.3 ± 0.1 | 21.0 ± 9.0 | 31.3 ± 7.9 | 14.3 ± 1.3 |
Summer | 17 | 5.7 ± 0.0 | 3.4 ± 0.0 | 0.3 ± 0.0 | 12.7 ± 2.5 | 7.7 ± 0.2 | 0.3 ± 0.0 | 12.8 ± 4.8 | 20.7 ± 4.9 | 11.9 ± 1.0 | |||
Downstream (Bali) | Winter | 20 | 5.6 ± 0.0 | −196 ± 16 | clay loam | 2.0 ± 0.1 | 0.3 ± 0.0 | 9.6 ± 2.0 | 6.5 ± 1.7 | 0.4 ± 0.1 | 18.3 ± 5.0 | 25.1 ± 6.2 | 32.6 ± 3.1 |
Summer | 21 | 6.2 ± 0.0 | 2.9 ± 0.0 | 0.3 ± 0.0 | 6.4 ± 3.8 | 54.2 ± 0.7 | 0.3 ± 0.0 | 27.7 ± 15.3 | 82.2 ± 15.4 | 20.7 ± 3.7 |
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Shiau, Y.-J.; Lin, C.-W.; Cai, Y.; Jia, Z.; Lin, Y.-T.; Chiu, C.-Y. Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan. Microorganisms 2020, 8, 1248. https://doi.org/10.3390/microorganisms8081248
Shiau Y-J, Lin C-W, Cai Y, Jia Z, Lin Y-T, Chiu C-Y. Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan. Microorganisms. 2020; 8(8):1248. https://doi.org/10.3390/microorganisms8081248
Chicago/Turabian StyleShiau, Yo-Jin, Chiao-Wen Lin, Yuanfeng Cai, Zhongjun Jia, Yu-Te Lin, and Chih-Yu Chiu. 2020. "Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan" Microorganisms 8, no. 8: 1248. https://doi.org/10.3390/microorganisms8081248