Differential miRNA-Gene Expression in M Cells in Response to Crohn’s Disease-Associated AIEC
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strain
2.2. Cell Lines and Cell Culture
2.3. Bacterial Translocation across M Cells
2.4. Total RNA Isolation from M Cells and Caco-2 Monolayers, Microarray, and miRNA Array
2.5. In Silico Analysis
3. Results
3.1. AIEC Bacteria Are Able to Translocate across Human M-Cell Monolayers
3.2. M-Cell Monolayers Exhibit Distinct miRNA and Gene Profiles Compared to Intestinal Epithelial Caco-2-cl1 Cells
3.3. AIEC Infection Induces Dysregulation of miRNAs in IECs and M Cells
3.4. In Silico Analysis to Predict the Potential Target Genes of Dysregulated miRNAs in IECs and M Cells during AIEC Infection
3.5. AIEC Infection Induces Gene Dysregulation in IECs and M Cells
3.6. MiRNA-Mediated Gene Regulation in IECs and M Cells during AIEC Infection
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AIEC | Adherent-invasive E. coli |
ATG16L1 | Autophagy related 16 like 1 |
BMP2 | Bone morphogenetic protein 2 |
Caco-2-cl1 | Caco-2 clone 1 |
CD | Crohn’s disease |
CFUs | Colony-forming units |
EOMES | Eomesodermin |
FAE | Follicle associated epithelium |
IECs | Intestinal epithelial cells |
IGF | Insulin-like growth factor |
LB | Luria–Bertani |
LPF | Long polar fimbriae |
M cells | Microfold or membranous cells |
MEF2D | Myocyte enhancer factor 2d |
miRNA, miR | MicroRNA |
NAMPT | Nicotinamide phosphoribosyltransferase |
NOD2 | Nucleotide-binding oligomerization domain 2 |
SNP | Single nucleotide polymorphism |
TBX3 | T-box transcription factor 3 |
TEER | Transepithelial electrical resistance |
TLR | Toll-like receptors |
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Larabi, A.; Salesse, L.; Cordonnier, C.; Etienne-Mesmin, L.; Barnich, N.; Dalmasso, G.; Nguyen, H.T.T. Differential miRNA-Gene Expression in M Cells in Response to Crohn’s Disease-Associated AIEC. Microorganisms 2020, 8, 1205. https://doi.org/10.3390/microorganisms8081205
Larabi A, Salesse L, Cordonnier C, Etienne-Mesmin L, Barnich N, Dalmasso G, Nguyen HTT. Differential miRNA-Gene Expression in M Cells in Response to Crohn’s Disease-Associated AIEC. Microorganisms. 2020; 8(8):1205. https://doi.org/10.3390/microorganisms8081205
Chicago/Turabian StyleLarabi, Anaïs, Laurène Salesse, Charlotte Cordonnier, Lucie Etienne-Mesmin, Nicolas Barnich, Guillaume Dalmasso, and Hang Thi Thu Nguyen. 2020. "Differential miRNA-Gene Expression in M Cells in Response to Crohn’s Disease-Associated AIEC" Microorganisms 8, no. 8: 1205. https://doi.org/10.3390/microorganisms8081205