Avirulent Isolates of Penicillium chrysogenum to Control the Blue Mold of Apple Caused by P. expansum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolate Propagation, Media, and Storage
2.2. Virulence Assays in Apple Fruit
2.3. Fungal Germination and Mycelial Growth Assessment
2.4. Evaluation of Antimicrobial Resistance
2.5. Fungal DNA Extraction and Sequencing
2.6. Fungal Genome Assembly and Characterization
2.7. Statistical Analyses
2.8. Phylogenetic Tree
3. Results
3.1. Isolates 404 and 413 Are Identified as Penicillium chrysogenum
3.2. Penicillium chrysogenum Isolates Are Non-Pathogenic in Apple Fruit
3.3. Penicillium chrysogenum Isolates Have Comparable Growth Rate to P. expansum In Vitro
3.4. Penicillium chrysogenum Isolates Reduce Penicillium expansum Development in Apple Fruit
3.5. Penicillium chrysogenum Isolates Do Not Inhibit the Growth of Penicillium expansum In Vitro
3.6. Penicillium chrysogenum Isolates Are Resistant to Hygromycin and Patulin
3.7. P. chrysogenum Isolates Do Not Possess an Intact Canonical Patulin Biosynthetic Cluster
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Fungal Isolate | Patulin | Hygromycin | Pathogenicity in Apple Fruit |
---|---|---|---|
Penicillium expansum R19 | Resistant | Susceptible | Yes |
Penicillium expansum Pe21 | Resistant | Susceptible | Yes |
Penicillium chrysogenum isolate 404 | Resistant | Resistant | No |
Penicillium chrysogenum isolate 413 | Resistant | Resistant | No |
Penicillium chrysogenum DTO 275-D7 | Not tested | Resistant | No |
Penicillium rubens DTO 346-D4 | Not tested | Resistant | No |
Penicillium chrysogenum DTO 382-H5 | Not tested | Resistant | No |
Penicillium rubens DTO 397-I3 | Not tested | Resistant | No |
Penicillium chrysogenum DTO 402-A9 | Not tested | Resistant | No |
Penicillium rubens DTO 405-A6 | Not tested | Resistant | No |
Penicillium chrysogenum DTO 431-E3 | Not tested | Resistant | No |
Sequenced Isolate | Raw Reads | % GC | Reads Mapped to Genome Reference a | Barcode Loci b | Identification |
---|---|---|---|---|---|
Penicillium isolate 404 | 14,127,414 | 48.0 | 12,628,224 | BenA, ITS, rpb1, rpb2, CaM | P. chrysogenum |
Penicillium isolate 413 | 17,801,280 | 47.9 | 15,883,217 | BenA, ITS, rpb1, rpb2, CaM | P. chrysogenum |
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Bartholomew, H.P.; Luciano-Rosario, D.; Bradshaw, M.J.; Gaskins, V.L.; Peng, H.; Fonseca, J.M.; Jurick, W.M., II. Avirulent Isolates of Penicillium chrysogenum to Control the Blue Mold of Apple Caused by P. expansum. Microorganisms 2023, 11, 2792. https://doi.org/10.3390/microorganisms11112792
Bartholomew HP, Luciano-Rosario D, Bradshaw MJ, Gaskins VL, Peng H, Fonseca JM, Jurick WM II. Avirulent Isolates of Penicillium chrysogenum to Control the Blue Mold of Apple Caused by P. expansum. Microorganisms. 2023; 11(11):2792. https://doi.org/10.3390/microorganisms11112792
Chicago/Turabian StyleBartholomew, Holly P., Dianiris Luciano-Rosario, Michael J. Bradshaw, Verneta L. Gaskins, Hui Peng, Jorge M. Fonseca, and Wayne M. Jurick, II. 2023. "Avirulent Isolates of Penicillium chrysogenum to Control the Blue Mold of Apple Caused by P. expansum" Microorganisms 11, no. 11: 2792. https://doi.org/10.3390/microorganisms11112792